Melanopsin and rod–cone photoreceptive systems account for all major accessory visual functions in mice

doi:10.1038/nature01761

Nature. Author manuscript; available in PMC 2010 June 15.

Published in final edited form as:

Nature. 2003 July 3; 424(6944): 76–81.

Published online 2003 June 15. doi: 10.1038/nature01761

PMCID: PMC2885907

NIHMSID: NIHMS204472

Melanopsin and rod–cone photoreceptive systems account for all major accessory visual functions in mice

S. Hattar,^* R. J. Lucas,^† N. Mrosovsky,^‡ S. Thompson,^† R. H. Douglas,^§ M. W. Hankins,^† J. Lem,^‖ M. Biel,^¶ F. Hofmann,^# R. G. Foster,^† and K.-W. Yau^*

^*Howard Hughes Medical Institute and Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA

^†Department of Integrative and Molecular Neuroscience, Division of Neuroscience and Psychological Medicine, Faculty of Medicine, Imperial College London, Charing Cross Campus, London W6 8RF, UK

^‡Departments of Zoology, Physiology and Psychology, University of Toronto, Toronto, Ontario M5S 3G5, Canada

^§Applied Vision Research Centre, Department of Optometry and Visual Science, City University, Northampton Square, London EC1V 0HB, UK

^‖Department of Ophthalmology, Tufts University School of Medicine, Boston, Massachusetts 02111, USA

^¶Lehrstuhl Pharmakologie für Naturwissenschaften, Zentrum für Pharmaforschung, Ludwig-Maximilians Universität München, 81377 München, Germany

^#Institut für Pharmakologie und Toxikologie, Technische Universität München, 80802 München, Germany

Correspondence and requests for materials should be addressed to K.-W.Y. (Email: kwyau/at/mail.jhmi.edu)

The publisher's final edited version of this article is available at Nature

See the article"Targeted Mutation of the Calbindin D_28K Gene Disrupts Circadian Rhythmicity and Entrainment" in Eur J Neurosci, volume 5 on page 2907.

See other articles in PMC that cite the published article.

Abstract

In the mammalian retina, besides the conventional rod–cone system, a melanopsin-associated photoreceptive system exists that conveys photic information for accessory visual functions such as pupillary light reflex and circadian photo-entrainment¹^–⁷. On ablation of the melanopsin gene, retinal ganglion cells that normally express melanopsin are no longer intrinsically photosensitive⁸. Furthermore, pupil reflex⁸, light-induced phase delays of the circadian clock⁹^,¹⁰ and period lengthening of the circadian rhythm in constant light⁹^,¹⁰ are all partially impaired. Here, we investigated whether additional photoreceptive systems participate in these responses. Using mice lacking rods and cones, we measured the action spectrum for phase-shifting the circadian rhythm of locomotor behaviour. This spectrum matches that for the pupillary light reflex in mice of the same genotype¹¹, and that for the intrinsic photosensitivity of the melanopsin-expressing retinal ganglion cells⁷. We have also generated mice lacking melanopsin coupled with disabled rod and cone photo-transduction mechanisms. These animals have an intact retina but fail to show any significant pupil reflex, to entrain to light/dark cycles, and to show any masking response to light. Thus, the rod–cone and melanopsin systems together seem to provide all of the photic input for these accessory visual functions.

Rods and cones have long been thought to be the exclusive photoreceptors in the retina. This hypothesis is now known to be untrue. An opsin-like protein called melanopsin, originally identified in Xenopus skin melanophores¹, is present in a small subset of mammalian retinal ganglion cells (RGCs)¹^–⁶, and these cells are intrinsically photosensitive⁶^,⁷. The axons of these RGCs project predominantly to the suprachiasmatic nucleus (SCN), the intergeniculate leaflet (IGL) and the olivary pretectal nucleus (OPN) of the brain⁶, which are key centres for circadian photo-entrainment and the pupillary light reflex. In melanopsin knockout mice (Opn4^−/−, formerly referred to as mop^−/−; see ref. ⁸), those RGCs that would normally express melanopsin lose their intrinsic photosensitivity⁸. Opn4^−/− mice also have an incomplete pupillary light reflex at high illuminations⁸. In independently produced melanopsin-knockout mice, others have found that the ability of light to phase-delay and lengthen the period of the circadian rhythm is also diminished⁹^,¹⁰. For the pupil reflex, this photic response can be quantitatively accounted for by a functional complementarity between the rod–cone system and the melanopsin system, without the need to invoke any additional light-detection system⁸. Nonetheless, the proposal has persisted that cryptochromes—flavoproteins reported to have a direct light-detecting role in Drosophila¹²^,¹³—may have the same function in mammals¹⁴^–¹⁶ despite earlier evidence to the contrary¹⁷. To settle this question, we first examined the action spectrum for phase-shifting the circadian rhythm in mice lacking rod and cone photoreceptors (rd/rd cl)¹⁸. Next, we generated triple-knockout mice lacking all confirmed photodetection systems—Opn4^−/− Gnat1^−/− Cnga3^−/− (melanopsin (also known as opsin 4), guanine nucleotide-binding protein α-transducin 1 (also known as rod transducin α-subunit, or Trα) and cyclic GMP-gated channel A-subunit 3, respectively)—and tested these animals for pupil reflex, circadian photo-entrainment and the masking response to light.

Irradiance–response relations for the light-induced phase shifting of the circadian rhythm of locomotor behaviour in rd/rd cl mice were measured at various wavelengths (Fig. 1a). The irradiance for half-maximal phase shift at each wavelength was then plotted to give the action spectrum (Fig. 1b). This spectrum is best fitted by the predicted absorption spectrum of a vitamin A₁-based photopigment with a wavelength of maximum absorbance (λ_max) ≈ 481 nm, similar to that for the pupil reflex in this genotype (λ_max ≈ 479 nm)¹¹ and, more specifically, that for the intrinsically photosensitive, melanopsin-expressing RGCs in the rat (λ_max ≈ 484 nm)⁷. Thus in the absence of rods and cones, circadian photo-entrainment is apparently determined by the melanopsin system. Previously, an action spectrum with a λ_max of 480 nm has been reported for circadian phase shifting by light in one strain of rd mice that has lost rods and perhaps most cones³¹. However, in part because the same experiments on a different strain of rd mice gave a λ_max between 500 nm and 515 nm (ref. ³²) and interpreted to reflect the action spectrum of mouse middle-wavelength-sensitive (M)-cones, the significance of the first study was never settled. With the rd/rd cl mouse line described here in which the loss of rods and cones is essentially complete, however, the signal from residual cones is no longer an issue.

Figure 1

Irradiance–response relations (a) and action spectrum (b) for circadian phase shifting in rd/rd cl mice by monochromatic light between 420 nm and 580 nm, assayed by wheel-running (n = 4–7 animals per irradiance at each wavelength). The (more ...)

To obtain more conclusive evidence that no other independent photoreceptive systems exist for the various accessory visual functions, we generated triple-knockout mice in which the rod–cone system and the melanopsin system were both silenced. Notably, the silencing of rod–cone functions in this case was achieved not by inducing degeneration of these cells (as occurs in rd/rd cl mice) but by combining targeted deletions of the genes for rod Gnat1 (ref. ¹⁹) and cone Cnga3 (refs ²⁰^,²¹). Gnat1 and Cnga3 are critically involved in the G-protein-coupled cGMP signalling pathway that mediates rod–cone phototransductions. Therefore, in triple-knockout mice, the melanopsin system and the rod–cone system are both unable to signal light. We investigated whether these animals still had any residual response to light, as assayed by their pupil reflex and locomotor behaviour.

The Opn4^−/− Gnat1^−/− Cnga3^−/− mice were generated by first producing triple heterozygotes (Opn4^+/− Gnat1^+/− Cnga3^+/−) and then mating these to homozygosity. These mice had a normal-looking retina (Fig. 2a). Because Opn4^−/− was produced by replacing the melanopsin gene with the tau-LacZ construct⁶^,⁸, the RGCs that would normally express melanopsin could be visualized by 5-bromo-4-chloro-3-indolyl-β-d-galactoside (X-gal) labelling. In the triple-knockout mice, these cells were still present (Fig. 2b) in numbers (about 600 per retina) comparable to wild type⁶^,⁸, and their axons still projected predominantly to the SCN, IGL and OPN of the brain (Fig. 2c–e). Thus, the absence of melanopsin and of functional rod–cone phototransductions does not affect the genesis, survival and central connectivity of the melanopsin-associated RGCs.

Figure 2

Normal retinal morphology and presence and central connectivity of melanopsin-expressing RGCs in triple-knockout (Opn4^−/− Gnat1^−/− Cnga3^−/−) mice. a, Retinal cross-sections from Opn4^+/− and triple-knockout (more ...)

When tested for the pupillary light reflex with steady, intense exposure of light at 480 nm, the triple-heterozygous mice gave a response resembling wild type in amplitude and time course (Fig. 3a). The double-knockout mice (Gnat1^−/− Cnga3^−/−) showed the same response (Fig. 3a), similar to what we found previously¹¹ for rd/rd cl mice, which have complete degeneration of rods and cones. Triple knockouts, however, hardly gave any response to the 480-nm stimulus (Fig. 3a), nor to monochromatic light at other wavelengths (360–660 nm) or intense white light (data not shown). The same general results were obtained when these animals were tested at various times over a 24-h period. On close inspection, there was a very transient, barely detectable pupil response (with a mean of 5% reduction in pupil area at peak response) in two out of six tested triple-knockout mice under exposure to bright 480-nm light. However, even in animals where this residual response was detected, it was not consistently present on repeated stimulus trials with extensive dark adaptation (up to 3 days) in between (see Fig. 3 legend). The response was unlikely to be caused by heat associated with the illumination, because it largely disappeared after replacement of the 480-nm interference filter with a band-pass filter transmitting only infrared light (≥850 nm) (Fig. 3a). A possible source of the signal is the early receptor potential of rods and cones, which consists of a very small, transient membrane hyperpolarization caused by charge movements associated with conformational changes of the visual pigments after photo-isomerization²²^,²³. This hyperpolarization should persist regardless of whether the transduction steps downstream of the pigment are disabled or not. Another possibility is a very small, transient hyperpolarization generated by intense light in rods and cones via a pathway apparently independent of transducin²⁴. In any case, the smallness of the residual pupil response, its transient nature even in steady, intense light, and the inconsistency of its occurrence all suggest that it is unlikely to be of physiological significance. Finally, the failure of the Opn4^−/− Gnat1^−/− Cnga3^−/− pupil to constrict was not due to a defect in the constriction mechanism because carbachol, a parasympathetic agonist, was able to activate maximum pupil constriction when applied to the cornea (Fig. 3b).

Figure 3

Disabling of rods, cones and melanopsin-positive RGCs essentially eliminates the pupillary light reflex. a, Pupil area (mean ± s.e.m.) as a percentage of dark-adapted aperture area (recorded just before light exposure, time 0 on graph) for Opn4 (more ...)

To assess photo-entrainment, Opn4^+/− Gnat1^+/− Cnga3^+/− and Opn4^−/− Gnat1^−/− Cnga3^−/− animals separate from those used in the pupil-reflex studies were kept in an 16/8-h light/dark cycle (800 lx white light in the light period), and their locomotor activity was monitored (see Methods). The triple-heterozygous mice showed normal photo-entrainment, whereas the triple-knockout mice did not show any entrainment (Fig. 4; see also Supplementary Information). Actograms indicated that the triple-heterozygous mice had an average period length of 24.0 ± 0 h (mean ± s.e.m., n = 6; individually all 24.0 h), as expected from stable photo-entrainment. In contrast, the triple homozygous mice had a period length of 23.3 ± 0.2 h (n = 5; individually 23.8, 23.3, 23.2, 22.5 and 23.5 h), similar to the 23.6 h reported for the same strain (B6/129) of wild-type mice in constant darkness²⁵, indicating that these animals free-ran even under light/dark conditions. Of note, at the light intensity used in these experiments, the pupils of wild-type or triple-heterozygous mice would have constricted considerably (see Fig. 3a), whereas those of the triple-knockout mice would have stayed unchanged. Thus, the equivalent light intensity at which the triple-knockout mice failed to show photo-entrainment was actually much higher than that capable of entraining wild-type or triple-heterozygous mice.

Figure 4

Actograms of wheel running for mice under a 16/8-h light/dark cycle, double-plotted on a 24-h timescale. The illumination was approximately 800 lx white light. The numbered lines represent successive days. Activity levels (in total number of revolutions (more ...)

We have also examined another accessory visual function, namely the masking of locomotion of nocturnal rodents by light, which involves a fast and direct effect of light independent of the circadian pacemaker²⁶^–²⁸. To examine masking by light, the mice tested for photo-entrainment were subsequently placed on a 3.5/3.5-h light/dark cycle (800 lx white light as before, although the equivalent intensity for the triple-knockout mice would again be considerably higher than for the triple-heterozygous mice when the lack of pupil reflex in the triple-knockout animals is taken into account). This ultradian cycle is useful for assessing masking because it is difficult to entrain circadian rhythms to cycles with periods of 7 h or multiples thereof. In this way, it was ensured that masking could be measured at different phases of the endogenous clock²⁹. When kept in this cycle, the triple-heterozygous mice showed two effects. Four of the six tested animals were negatively masked (that is, their locomotor activity was diminished) by light (Fig. 5a). The average percentage of activity in the dark period for these ‘light avoiders’ was 87.1 ± 8.8% (mean ± s.e.m.; individually 99.5%, 66.3%, 85.0% and 97.7%), similar to what was seen with B6/129 wild-type mice (n = 12; N.M. and S.H., unpublished data). The remaining two animals, paradoxically, developed more wheel-running activity in the light period (Fig. 5b), possibly due to the presence of the 129-strain background associated with the triple-heterozygous genotype (this activity reversal has previously been observed in other mouse lines with the 129-strain background; R.J.L., unpublished data). Although the reason for this reversal of activity with respect to the light/dark cycle is unclear, undoubtedly light still had an effect (in this case, positive masking). The percentage of activity in the dark period for these two ‘light preferers’ was 32.7% (Fig. 5b) and 26.0%, respectively. The triple-knockout mice, on the other hand, were hardly affected by this ultradian light/dark cycle (Fig. 5c, d). The average percentage of activity in the dark period was 43.2 ± 3.4% (mean ± s.e.m., n = 4; individually 44.7%, 50.5%, 40.8% and 36.6%), much closer to that expected from randomness (50%).

Figure 5

Actograms of wheel running plotted on a 7-h timescale. The mice were subjected to an ultradian 3.5/3.5-h light/dark cycle (see text). Illumination was approximately 800 lx white light. The numbered lines represent successive 7-h cycles. The activity quantiles (more ...)

Given the fact that RGCs normally expressing melanopsin still project to the appropriate brain targets in triple-knockout mice, the conclusion from our experiments is that the rod–cone system and the melanopsin system are the exclusive light-detecting systems in the eye for producing the normal pupillary light reflex, photo-entrainment and masking response to light. These experiments go beyond our previous conclusion that the rod–cone and melanopsin systems are fully complementary to each other in the pupil reflex⁸, by demonstrating an essentially complete loss of this and other functions when the two systems are absent or disabled. It was recently reported¹⁶ that the pupil reflex of mice lacking rods as well as Cry1 and Cry2 (rd/rd Cry1^−/− Cry2^−/−) is 100-fold less photosensitive than normal (and 10-fold less sensitive than mice only lacking rods; rd/rd), proposed to support the idea that the cryptochromes may participate as a photodetective system. Nonetheless, other interpretations of these earlier data are possible. Furthermore, the pupil reflex of mice only lacking Cry1 and Cry2 is normal (as is their masking response to light³⁰). A potential complicating factor in interpreting the results from mice lacking Cry1 and Cry2 is that these animals are reported to show frequent ocular inflammation¹⁵, which may cause subtle changes in retinal function. In any case, we know from immunocytochemistry of our Opn4^−/− Gnat1^−/− Cnga3^−/− mice that at least Cry2 is still widely expressed in the inner retina (including the RGCs expressing melanopsin), comparable to wild type (data not shown)—the antibodies for Cry1 did not give informative labelling; see Methods. At the same time, real-time polymerase chain reaction with reverse transcription (RT–PCR) indicated that the message levels for Cry1 and Cry2 in Opn4^−/− Gnat1^−/− Cnga3^−/− eyes are comparable to those in wild-type mice (Supplementary Information). Thus, the disappearance of the accessory visual functions in the triple-knockout mice cannot be attributed to secondary loss of cryptochromes. Even if mammalian cryptochromes should turn out to function as direct light detectors, either they obligatorily depend on the presence of melanopsin for their light-detecting function or they do not signal light at least for the diverse photic responses studied here.

Methods

Animals

The rd/rd cl mice were bred at Imperial College London, and the circadian phase-shifting experiments were carried out there. The triple-knockout and the corresponding triple-heterozygous mice were bred at Johns Hopkins. The pupil reflex experiments were done at Hopkins, whereas the wheel-running experiments were done at the University of Toronto. At the time of experimentation, the Hopkins animals were 1–4 months old for the pupil reflex measurements and 1–9 months old (1–9 months for triple-heterozygous mice, 1–2 months for triple-knockout mice; all were males except for one female triple knockout, which was used for photo-entrainment but not for masking experiments) for the wheel-running measurements. For morphological, X-gal labelling, immunocytochemical and RT–PCR experiments, the animals were 2–3 months old.

Giemsa staining of retinal cross-sections

An animal anaesthetized by intraperitoneal injection of avertin (0.2 ml g⁻¹) was circulationally perfused with 25 ml cold 4% paraformaldehyde in PBS for 15 min. The eyes were isolated, corneas removed, and fixed for an additional 3 h in cold 4% paraformaldehyde in PBS. The eyes were cryo-protected overnight in 30% sucrose in PBS at 4 °C, then sectioned at 10 µm thickness on a cryostat. The sections were allowed to dry and stained with the nucleus-labelling Giemsa solution (Sigma).

Assays for Cry1 and Cry2 in triple-knockout eyes

We used two assays. The first was immunocytochemistry⁶, with antibodies PA1-527 (Affinity Bioreagents) and CRY11-A (Alpha Diagnostic International) against Cry1, and CRY21-A (Alpha Diagnostic International) against Cry2. The two antibodies against Cry1 did not give specific labelling in the retina, but Cry2 gave labelling specific enough for comparison with wild-type retina. The second assay was with RT–PCR using Cry1- and Cry2-specific primers. The samples were whole eyes without the lens, and RNA was extracted using the trizol method (Sigma). The controls were RNA samples without reverse transcriptase. Real-time RT–PCR was carried out with SYBR green detection on an ABI Prism 7700 machine (Applied Biosystems). The identity of the product was checked by melting-curve analysis, size confirmation on an agarose gel and sequencing of the products.

X-gal staining

For X-gal labelling of the brain sections or flat-mount retina of the triple-knockout mice, an animal was anaesthetized and circulationally perfused with fixative as above, and the brain/eyes were isolated. An eye-cup was prepared by removing the anterior half of the eye, while the brain was frozen on dry ice and sectioned at 50-µm thickness with a sliding microtome (Leica SM2000R). Brain sections were mounted on microscope slides (Fisherbrand, superfrost-plus) and allowed to dry overnight. To stain for β-galactosidase activity, both preparations were washed twice in buffer B (100 mM phosphate buffer, pH 7.4, 2 mM MgCl₂, 0.01% Na-desoxycholate and 0.02% (octylphenoxy) polyethoxyethanol (IGEPAL)), and then incubated in the staining solution (buffer B plus 5 mM K-ferricyanide, 5 mM K-ferrocyanide and 1 mg ml⁻¹ X-gal) for 6–10 h at room temperature in darkness. Afterwards, the retina was isolated from the eye-cup and flat mounted. Both brain sections and the retina were mounted in glycerol. Images were obtained using a Zeiss Axiophot microscope and AxioCam HRc digital camera.

Phase shifting of locomotion in rd/rd cl mice

Singly housed male C3H/He rd/rd cl mice aged between 80 and 250 days were maintained in constant darkness. A single 15-min monochromatic light pulse (half bandwidth = 10 nm) of defined irradiance was applied after 7 days at circadian time 16 (4 h after activity onset) at 420, 460, 471, 506, 540, 560 and 580 nm, respectively. To correct for lens transmission at different wavelengths, lenses were extracted from rd/rd cl mice (n = 4) and wavelength-scanned in air using an integrating sphere (see Supplementary Information). The irradiances indicated have been corrected for lens transmission. The magnitude of phase shift in free-running locomotor activity rhythm by light at each wavelength was plotted against irradiance and fitted to a sigmoidal function. The irradiances that produced half-maximum phase shifts were plotted against wavelength to produce the action spectrum. This spectrum was fitted with the absorption spectrum of a vitamin A₁-based pigment with a best-fit λ_max determined by least squares.

Pupillometry

Consensual pupillary constriction was measured in response to an adirectional light stimulus. Light was provided by a PTI xenon arc-lamp, filtered to remove wavelengths <299 nm and most infrared, and transmitted by means of a liquid light guide to a diffusing sphere. This sphere was constructed from a ping-pong ball and designed to render the light stimulus nearly adirectional at the location of the mouse cornea. An adult mouse, dark-adapted for 1 h and unanaesthetized, was positioned and oriented with the help of an infrared image-converter such that one eye was situated at the exit of the reflective sphere and the other eye was in focus, and oriented as perpendicular as possible, to a charge-coupled device (CCD) camera fitted with a × 10 macro lens. Illumination of the videoed eye was with infrared LEDs (>850 nm) throughout the experiment. Pupil measurements from the video images of the eye were made using TINA 3.1 image-analysis software and a Matrox meteor-II frame-grabber card (Matrox Imaging). The intensity and wavelength of the light stimulus were controlled with calibrated neutral-density and interference (10-nm bandwidth) filters. Irradiance measurements (W cm⁻²) were made with a radiometer (Macam Photometrics). Unless indicated otherwise, all experiments reported here were conducted during the light period of a 14/10-h light/dark cycle (between 9 and 3 h before lights off)⁸.

Carbachol application

Carbachol (carbamylcholine chloride) was obtained from Sigma. Solution (100 mM) was prepared in × 1 PBS, pH 7.4, and filtered through a 0.22-µm filter. Samples were topically applied to the cornea of the mouse eye with a microtip transfer pipette. The pupil size in darkness was assessed immediately before and at 5 min after carbachol application. We found that a carbachol concentration of 100 mM was able to fully constrict the eyes of both triple-heterozygous and triple-knockout mice, and therefore we did not use higher concentrations (see ref. ⁸).

Photo-entrainment and masking experiments

The mice were placed individually in cages (44 × 23 × 20 cm) equipped with running wheels (17.5 cm in diameter). Room temperature was 19.5–23.5 °C. Revolutions were recorded with Dataquest III hardware and software in 10-min bins. The light/dark cycle was 16/8-h light/dark or 3.5/3.5-h light/dark, with approximately 800 lx in the light period (Hagner EX2 luxmeter) illuminated by Sylvania Octron 32 W 4100K fluorescent tubes. For photo-entrainment, the animals were studied for 19 days on the 16/8-h light/dark cycle, with data from the last 10 days used for period analysis based on the algorithm of Clocklab (Actimetrics) for the Bushell and Sokolove χ² periodogram. For masking, the animals were kept on the ultradian 3.5/3.5-h light/dark cycle for 11 days. Data from the last 7 days, which covered light and dark periods spanning the entire circadian cycle, were used for calculating the percentages of wheel-running activity in darkness.

Supplementary Material

Fig 1

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Fig 2

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Figure Legends, Methods

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Acknowledgements

We thank H.-W. Liao and J. Lai for help in performing the immunocytochemical experiments with antibodies against cryptochromes; J. Butler for help with real-time RT–PCR; Y. Liang for help in genotyping the animals; P. Salmon for help in the wheel-running experiments; and D. M. Berson, H. R. Matthews, G. L. Fain and members of the Yau laboratory for scientific discussions. This work was supported by the US National Eye Institute (K.-W.Y.), the UK Biotechnology and Biological Sciences Research Council and the Wellcome Trust (R.J.L. and R.G.F.), and the Canadian Institutes of Health Research (N.M.).

Footnotes

Supplementary Information accompanies the paper on www.nature.com/nature.

Competing interests statement The authors declare that they have no competing financial interests.

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