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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Neurosci. Author manuscript; available in PMC 2010 November 12.
Published in final edited form as:
PMCID: PMC2881304
NIHMSID: NIHMS204169

Altered GABAA receptor-mediated synaptic transmission disrupts the firing of gonadotropin-releasing hormone neurons in male mice under conditions that mimic steroid abuse

Abstract

Gonadotropin–releasing hormone (GnRH) neurons are the central regulators of reproduction. GABAergic transmission plays a critical role in pubertal activation of pulsatile GnRH secretion. Self-administration of excessive doses of anabolic androgenic steroids (AAS) disrupts reproductive function and may have critical repercussions for pubertal onset in adolescent users. Here, we demonstrate that chronic treatment of adolescent male mice with the AAS, 17α-methyltestosterone (17αMT), significantly decreased action potential frequency in GnRH neurons, reduced the serum gonadotropin levels, and decreased testes mass. AAS treatment did not induce significant changes in GABAA receptor subunit mRNA levels or alter the amplitude or decay kinetics of GABAA receptor-mediated spontaneous postsynaptic currents (sPSC) or tonic currents in GnRH neurons. However, AAS treatment significantly increased action potential frequency in neighboring medial preoptic area (mPOA) neurons and GABAA receptor-mediated sPSC frequency in GnRH neurons. In addition, physical isolation of the more lateral aspects of the mPOA from the medially-localized GnRH neurons abrogated the AAS-induced increase in GABAA receptor-mediated sPSC frequency and the decrease in action potential firing in the GnRH cells. Our results indicate that AAS act predominantly on steroid-sensitive presynaptic neurons within the mPOA to impart significant increases in GABAA receptor-mediated inhibitory tone onto downstream GnRH neurons resulting in diminished activity of these pivotal mediators of reproductive function. These AAS-induced changes in central GABAergic circuits of the forebrain may significantly contribute to the disruptive actions of these drugs on pubertal maturation and the development of reproductive competence in male steroid abusers.

Keywords: GnRH neurons, medial preoptic area, anabolic androgenic steroids, 17α methyl-testosterone, GABAA receptor, luteinizing hormone, androgen receptor, adolescence

Introduction

The AAS are synthetic derivatives of testosterone whose therapeutic use has been overshadowed by illicit self-administration of excessive doses of these drugs resulting in adverse effects on reproduction and sexual behaviors (Kam and Yarrow, 2005). AAS abuse by teenagers (Irving et al., 2002; Kanayama et al., 2008) may present even greater risks than for adults, given the greater hormone sensitivity of the brain during adolescence (Sato et al., 2008). Administration of supraphysiological levels of AAS in rodents alters pubertal onset and reproductive behaviors and is associated with suppression of gonadotropin secretion and disruption of the hypothalamic-pituitary-gonadal (HPG) axis (Clark and Henderson, 2003). Activity of GnRH neurons, which constitute the epicenter of HPG control, is highly sensitive to changes in physiological gonadal steroids (Kelly and Wagner, 2002; Moenter et al., 2003; Pielecka and Moenter, 2006; Clarkson and Herbison, 2009), and AAS-dependent changes in their activity may disrupt reproductive function in adolescence.

The AAS comprise a chemically diverse group of steroids that can signal via androgen receptors (AR), and, following aromatization, via estrogen receptor (ER) α and β. While ERβ mRNA and protein has been measured in GnRH neurons of male rodents (Hrabovszky et al., 2001), ERα mRNA is minimally expressed in these cells (Hu et al., 2008) and AR has not been detected. In contrast, neighboring non-GnRH neurons of the mPOA, the majority of which are GABAergic (Gao and Moore, 1996; Sagrillo and Selmanoff, 1997), express robust levels of AR and ERα and moderate levels of ERβ (Lu et al., 1998; Mitra et al., 2003; Nomura et al., 2003; Kudwa et al., 2004; Shah et al., 2004). GnRH neuronal somata in male mice are nestled within a dense surround of GABAergic neurons (Turi et al., 2003; Chen and Moenter, 2009), and neurons within the mPOA and neighboring bed nucleus of the stria terminalis (BnST) are likely sources of these GABAergic afferents in male rodents (Simerly and Swanson, 1988; Hutton et al., 1998). These prior studies have led to the consensus that the effects of gonadal steroids on GnRH function are predominantly indirect (Scott et al., 2000; Grattan et al., 2007; Herbison, 2008), with GABAergic neurons that supply afferents to the GnRH neurons being among the prime candidates as the targets for steroid action. While important links between gonadal steroids, GABAergic tone in the mPOA, GnRH neuronal activity, and HPG function have been established in adult male rodents (Grattan et al., 1996; Mitsushima et al., 1999, 2003; Tin-Tin-Win-Shwe et al., 2002, 2004; Pielecka and Moenter, 2006; Chen and Moenter, 2009), the impact of exposure to supraphysiological levels of synthetic androgens during adolescence is unknown. Here we demonstrate that chronic treatment of adolescent male mice with AAS imparts significant effects on GABAergic inputs to GnRH neurons and on the activity of these final effector cells. Such changes are likely to play an important role in the ability of the AAS to disrupt pubertal onset and reproductive maturation during adolescent male development.

Methods

Drugs and reagents

17α-Methyltestosterone (17αMT) was purchased from Steraloids. All other drugs and reagents were from Sigma Chemical Corp. Salts used for making biological buffers and solutions were mainly from Fisher Scientific and Sigma Chemical Corp.

Animal care and use

Male transgenic mice in which the green fluorescent protein is driven from the gonadotropin releasing hormone promoter (GFP-GnRH mice) (Suter et al., 2000) were obtained from an in-house breeding colony at Dartmouth Medical School. GFP expression in this transgenic line allows accurate identification of GnRH neurons in the living tissue by fluorescence microscopy. In particular, 84 to 94% of GFP fluorescent-GnRH neurons have been shown to be immunopositive for GnRH labeling and are predominantly found within the medial and lateral portions of the mPOA (Suter et al., 2000). All animal care procedures were approved by Institutional Animal Care and Use Committee at Dartmouth, in agreement to the guidelines and recommendations of the National Institutes of Health and American Veterinary Medical Association. All animals were housed in a temperature controlled and 12 light cycle facility with lights on starting at 7 AM.

Drug treatment paradigm

The median age of steroid initiation in the human population is reported to be 15 years old, with appreciable initiation noted as early as 12 years of age (Bahrke et al., 2000); adolescent ages that precede completion of reproductive maturation. Assessments of the development of the HPG axis in male mice of comparable strain background to the GFP- GnRH animals used in this study suggest that changes associated with reproductive maturation occur as early as the 3rd–4th week of postnatal development. Specifically, LH levels increase from ~10 ng/ml at birth to ~90 ng/ml by PN24 (Selmanoff et al., 1977) and balanopreputial separation occurs by PN 27–28 (Keene et al., 2002). To determine the effects of AAS exposure during adolescence on reproductive maturation, male GFP-GnRH mice in this study were administered 7.5 mg/kg/day 17αMT in sesame oil 6 days a week beginning on day PN25–28 for a period of 4 weeks. This dosage reflects a high human abuse regime, alters the onset of puberty and inhibits reproductive behaviors in both male and female rats (Clark et al., 2006), and significantly alters testes and seminal vesicle weights in adolescent male mice (McIntyre et al., 2002). Control subjects were administered the same volume (10–30 µl based on body weight) of sesame oil alone. 17αMT was chosen as it is predominantly an androgenic AAS (AAS that are α-alkylated at C17 cannot be aromatized to 17β-estradiol) (Winters, 1990; Kochakian and Yesalis, 2000) and these compounds may partially inhibit aromatase in vitro (de Gooyer et al., 2003) and in vivo (Penatti et al., 2009b).

LH and FSH measurements

Quantification of serum levels of luteinizing hormone (LH) and follicle stimulating hormone (FSH) was made according to protocols described previously (Gay et al., 1970; Fallest et al., 1995). Sera from 3 different cohorts of control and AAS-treated animals were collected from animals also used for electrophysiological recording and were assayed in singlet via radioimmunoassay by the University of Virginia Ligand Assay Core Laboratory (http://www.healthsystem.virginia.edu/internet/crr/). Lower concentrations limits for the assays were 20 pg/ml for LH and 0.8 ng/ml for FSH and intra-assay CV was 4.9%.

Slice Preparation

Coronal sections were prepared using an Electron Microscopy Sciences OTS-4000® vibroslicer as described previously (Penatti et al., 2005; 2009a,b). Briefly, whole brains were quickly removed and placed in ice-cold oxygenated low-sodium solution consisting of (in mM): 250 sucrose, 1.2 CaCl2, 10 glucose, 4 KCl, 7 MgSO4, 26 NaHCO3, 1.25 NaH2PO4, and 1 ascorbic acid (pH 7.35) and 300 µm sections were cut that included the mPOA as defined by Franklin and Paxinos (1997). This area encompasses nearly all of the population of GFP-GnRH neurons, which are mainly localized in its anterior and medial limits. Single identified GFP-GnRH neurons within the mPOA from individual subjects were also harvested and pooled for reverse transcription coupled with quantitative real time polymerase chain reaction (qRT-PCR).

Electrophysiological recordings

Patch-clamp recordings were performed as previously described according to Penatti et al. (2005, 2009a,b). All recordings were made between 1400 and 1800 hrs at room temperature. Recordings of spontaneous action potential currents (AP) were made in the loose-patch on-cell configuration (Rseal = ~50 – 100MΩ). Slices were superfused with 95%O2/5%CO2-saturated artificial cerebrospinal fluid (aCSF; in mM): 125 NaCl, 1.2 CaCl2, 10 glucose, 4 KCl, 1 MgCl2, 26 NaHCO3, 1.25 NaH2PO4, and 1 of the antioxidant, ascorbic acid (pH 7.35; 20–22°C), and aCSF was also present in the pipette. APs were recorded for a minimum of 3 min for each experimental condition, resulting in an average total time of recording of 12–20 min for GnRH neurons. Data acquisition was started only after baseline parameters (Ihold, Rseal) were stable. Data were recorded to tape and subsequently digitized using Acquire 4.0 software. AP frequency and patterning were analyzed using software written locally by Brian L. Jones (Oregon Health& Science University, Portland OR) in MatLab 6.5 R13 (The Mathworks). Frequency analysis was derived from direct assessment of inter-spike interval and patterning was determined using autocorrelation analysis (Penatti et al., 2009a,b) and classified according to Bar-Gad et al. (2001) as regular, irregular or “bursty”. Assignment of autocorrelational profiles to these three groups was found to be correlated with the coefficient of variation of AP firing: the bursty pattern was characterized by high CVs (≥ 4), the irregular pattern with CVs between 0.5 and 2.5, and the regular pattern with CVs of ≤ 0.5. The designations of patterning based upon the autocorrelogram classifications were found to be in universal agreement of classifications of firing patterns as regular, irregular or bursty that were independently made from the raw data by an independent observer.

For recordings of GABAA receptor-mediated spontaneous postsynaptic currents (sPSCs) and tonic currents, recordings were made in aCSF supplemented with 2 mM kynurenic acid to block receptors for excitatory transmission in the whole-cell configuration at a holding potential (Vhold) of −70 mV (20–22°C) (Yang et al., 2002; Penatti et al., 2005; 2009a,b). The pipette solution consisted of (in mM): 153 CsCl, 1 MgCl2, 5 EGTA, and 10 HEPES, to which 2 MgATP was added just prior to each experiment. The identity of synaptic currents as GABAergic was confirmed in some experiments by demonstrating blockade of events by the selective GABAA receptor competitive antagonist, bicuculline (20 µM). To record miniature PSC (mPSCs), 1 µM tetrodotoxin (TTX) was added to the kynurenic acid-containing aCSF bath solution to block AP-dependent GABA release (Nusser et al., 1997; Hájos et al., 2000). Digitized data were analyzed using MiniAnalysis software (Synaptosoft). Averaged PSCs were analyzed for peak current amplitude (Ipeak), frequency, and decay kinetics (biphasic and fitted with two time constants, τ1 and τ2) or with a single weighted time constant (τw) as previously described (Yang et al., 2002; Penatti et al., 2005, 2009a,b). Average membrane capacitance was 20 pF, average holding current (Ihold) was 50 pA and the average series resistance (Rseries) was 19 mΩ. Recordings were only accepted for analysis if the seal resistance (> 1GΩ), access resistance (<25 MΩ) and Ihold (in the absence of drug application) did not change more than ± ~10% during the recording. The magnitude of tonic GABAA-receptor-mediated currents (Itonic) was estimated from the difference in the amplitude of the baseline holding current before and after addition of a saturating concentration of the competitive GABAA receptor antagonist, picrotoxin (PTX; 100 µM) (Farrant and Nusser, 2005; Jones et al., 2006). Acquisition of data in the presence of 100 µM PTX was initiated ~1 min following the perfusion of this drug into the bath and data were acquired for 3 to 4 min.

For physiological recordings during which we physically isolated GnRH neurons from neurons in the more lateral aspects of the mPOA neurons, a unilateral linear and longitudinal cut was made under fluorescence optics using a stainless steel fine-point micro-scalpel with a 4 mm edge (Fine Science Tools). The cut was made parallel to the third ventricle border, ran throughout the mPOA lateral to the pool of the GnRH neurons, and ended dorsally at the limits of the anterior commissure (this area is called the “disrupted mPOA” throughout the text). A minimum distance of ~50 µm between the lateral-most GnRH neurons and the cut was maintained in order to preserve the viability/health of the GnRH neurons within the more medial aspect of the slice. Recordings from non-GnRH neurons in the mPOA were made from the central region of the medial preoptic nucleus (MPN) corresponding to the dorsal aspect of the MPN-medial and encompassing the MPN-central, as defined by Franklin and Paxinos (1997: Figures 29–32; see also Penatti et al., 2005). For assessment of the effects of lateral isolation on AP firing frequency, recordings were made from the disrupted mPOA and the intact, contralateral mPOA of the same slice for both control (oil-injected) and AAS-treated animals. Thus, the contralateral intact side served as an internal control for the effects of the cut irrespective of treatment condition. Data were analyzed only when recordings were successfully made from GnRH neurons from both the disrupted and the corresponding intact sides from a given mPOA slice. The low probability of meeting acceptable criteria inherent in this procedure resulted in one “pair” of successful recordings (i.e., one GnRH neuron on the cut side and one on the uncut side) per animal per condition. For experiments in which we assessed the effects of PTX on AP activity, independent recordings were made on a disrupted mPOA in one slice and an intact mPOA in a separate slice in order to avoid potential artifacts arising from the prolonged duration of these recordings or inefficient washout of PTX.

Single cell qRT-PCR of GnRH neurons

GFP-GnRH neurons were harvested between 1400 and 1800 hrs from coronal brain slices using borosilicate glass patch pipettes (Sutter Instrument Co.; Novato, CA) with tip diameters of approximately 7 to 10 µm. The pipettes were filled with aCSF containing 0.5U/µl RNase inhibitor (Ambion). The GnRH neurons were identified based on their fluorescence in the brain slice under a BX50 fluorescence microscope. Cell debris and tissue contaminants were avoided by aiming for superficial and healthy GnRH neurons (i.e., cells for which fluorescence was stable until the pipette approach and from which processes were partially visible as emerging from the soma). The GnRH neuron harvest was done for matched pairs of control and AAS-treated animals harvested on the same day. For each PCR sample, 5 GnRH neurons from one animal (typically from the same slice although in some instances two slices per animal were required) were collected into a 0.5 ml microcentrifuge tube containing 49.5µl cell lysis buffer + 0.5µl DNaseI provided in Ambion’s TaqMan®PreAmp Cells-to-CT™ Kit, followed by cDNA reverse transcription, cDNA pre-amplification (PreAmp) and quantitative real time PCR for the GABAA receptor subunit mRNAs. cDNA synthesis and qRT-PCR were carried out using minor modifications of procedures described by Li et al. (2008) using Ambion’s TaqMan®PreAmp Cells-to-CT™ Kit.. Briefly, the collected cells were lysed at room temperature for 5 min, the reaction was stopped by the addition of 5 µl Stop Solution and the mixture was incubated at room temperature for 2 min. RNA (22.5µl) was reverse transcribed for 60 min. at 37 °C in a total reaction volume of 50 µl that contained 1X RT buffer and 1X RT Enzyme Mix followed by enzyme inactivation for 5 min 95 °C. In a 50 µl reaction volume, 12.5 µl of cDNA from 5 single cells was pre-amplified in a reaction solution containing 25 µl TaqMan PreAmp mix, 0.2X pooled primer/probe sets (TaqMan Gene Expression Assays) commercially available for the GABAA receptor subunits α1 (Mm00439040_m1), α2 (Mm00433435_m1), α5 (Mm00621092_m1), β1 (Mm00433461_m1), β2 (Mm00549788_s1), β3 (Mm00433473_m1), δ (Mm00433476_m1), e (Mm00489932_m1), γ1 (Mm00439047_m1) and γ2 (Mm00433489_m1), GnRH (Mm01315605_m1) and β-actin as the endogenous control (Mm00607939_s1) (ABI). The reaction was pre-amplified with an initial denaturation step for 10 min 95 °C followed by 10 cycles of PCR at 95 °C 15 sec, 60 °C 4 min. Preamplified product was diluted 1:5 with 1X TE and qRT-PCR was performed for each TaqMan Gene Expression Assay individually. 20µl reaction containing 1X TaqMan Gene Expression Master Mix, 1X TaqMan Gene Expression Assay and 5 µl diluted PreAmp product was run on an ABI 7500 real-time instrument with an initial denaturation step for 10 min 95°C followed by 40 cycles at 92°C 15 sec, 60° 1 min. The 2−ΔΔCT method (Livak and Schmittgen, 2001; Peirson et al., 2003) was used for quantification of subunit mRNA. All primers used were demonstrated according to protocols in the TaqMan®PreAmp Cells-to-CT kit to amplify with equal efficiencies. Samples with reverse transcriptase omitted were used to control for genomic DNA contamination and a no template control to control for any reagent contamination. GnRH transcripts were always detected in samples of GnRH neurons and these transcripts were not detected in single cell harvests made from non-GnRH neurons. Similarly, GnRH transcripts were not amplified from mock harvests in which the pipette was placed on the surface of the GnRH neuron or in samples of aCSF solution superfusing the slices.

Statistical analyses

Values are presented as means ± standard error. To test for normality, Shapiro-Wilks or Kolmogorov-Smirnov tests were applied on the raw data. For serum LH and FSH assays, normally distributed values were accepted that fell within the 1st and 3rd quartile of all analyzed samples from each group. For qRT-PCR analysis, CT values were defined as outliers when they lay outside ± 3 standard deviations from the mean. Results were qualitatively the same whether or not outliers were included in the final analysis. Differences in the relative abundance of each mRNA between control and AAS-treated subjects were assessed using Pair-Wise Fixed Reallocation Randomization© t-test using the excel-based Relative Expression Software Tool (REST©; Pfaffl 2001; Pfaffl et al., 2002). For mRNA analysis, only positive error bars are depicted in the results; positive and negative error bars differed by ≤ 20%. For electrophysiological experiments, non-normally distributed data were log-transformed prior to statistical assessment. Significance for electrophysiological data and gonadotropin (LH/FSH) measurements was determined by one-way analysis of variance (ANOVA) followed by post hoc analyses using either Tukey or Fisher tests for means comparison (Origin8Pro; OriginLab). The same statistical tests were directly applied on all normally distributed data as well. For all data, the alpha level was set at p < 0.05. Except where indicated to the contrary, n values indicate the number of neurons per condition.

Results

I. AAS treatment decreases electrical activity in GnRH neurons and lowers serum LH and FSH of male mice

On-cell recordings from GnRH neurons within the mPOA of male mice were performed to assess spontaneous AP currents. Following AAS treatment, the average AP frequency in GnRH neurons was significantly decreased (p = 2.03 × 10−4) from 1.15 ± 0.25 Hz in control animals to 0.36 ± 0.06 Hz in AAS-treated mice (Figure 1). Autocorrelational analysis demonstrated bursty firing patterns were evident in ~61% and irregular firing patterns in 39% of the GnRH neurons from control subjects. AAS treatment did not significantly alter the relative proportion of cells with bursty versus irregular firing (73% and 27%, respectively). While the average frequency of AP firing was decreased with AAS treatment for cells with both patterns of firing, the decrease attained significance only for cells with bursty patterning (p = 4.19 × 10−5). The AAS-dependent decrease in firing frequency in bursty GnRH neurons correlated with a diminution in the number of APs per burst from 10.4 ± 3.0 in GnRH neurons from control subjects to 6.6 ± 0.8 in GnRH neurons from AAS-treated subjects. Consistent with prior reports, intra- and inter-burst intervals were variable (Kelly and Wagner, 2002), and there was no effect of AAS treatment on these parameters.

Figure 1
AAS-dependent effects on AP firing in GnRH neurons from control and AAS-treated mice

The pulsatile secretion of LH and FSH and its regulation of reproductive function are dependent on the frequency and patterning of electrical activity in GnRH neurons (Rosweir et al., 2009). Concomitant with the decrease in AP frequency in GnRH neurons, we observed lower levels of LH and FSH levels in AAS-treated versus control mice (Table 1). The observed decrease in FSH attained significance (p = 0.005). While the mean value of LH in AAS-treated animals was approximately 33% that observed in control animals (Table 1), these differences were not significant. The absence of statistical significance for serum LH levels arises from a large degree of inter-animal variability in both treated and control groups. This variability likely reflects the fact that blood was collected as a single point assay, but LH release in male rodents is pulsatile. Continuous serum sampling indicates that LH peaks occur with a frequency of tens of minutes to a few hours and that both peak amplitude and pulse frequency depend on the level of serum testosterone (Bartke et al., 1973; Steiner et al., 1982; Pierroz et al., 1999). Sampling at a single point from individual subjects in different phases of this pulsatile pattern will therefore introduce variability in the determination of mean LH levels. AAS treatment did result in a significant decrease in testes mass (Table 1; p = 7.506 × 10−4), consistent with an AAS-dependent diminution in the levels of both gonadotropins.

Table1
Serum gonadotropins and testes mass in control and AAS-treated male mice

II. AAS treatment alters presynaptic GABAergic inputs but not postsynaptic GABAA receptors in GnRH neurons

GnRH neurons are ubiquitously sensitive to GABA and express a broad repertoire of GABAA receptor subunit genes (Clarkson and Herbison, 2006). The most prevalent GABAA receptor subunit mRNAs expressed by GnRH neurons are reported to be α1, α2, α5, β1, β2, β3, γ1, γ2 (Sim et al., 2000; Pape et al., 2001, Todman et al., 2005), In addition to these transcripts, we also assessed levels of ε and δ subunit mRNAs in identified GnRH neurons. While levels of these ε and δ subunit mRNAs have not been previously assessed in GnRH neurons, immunocytochemical studies have indicated preferential expression of ε subunits in GnRH neurons and a limited number of other peptidergic cells (Moragues et al., 2003). Moreover, both ε- and δ-containing receptors may play important roles in receptors that mediated extrasynaptic tonic currents (Henderson, 2007). All of the GABAA receptor subunit mRNAs assayed were detected in identified GnRH neurons in both control and AAS-treated male mice. AAS treatment did not significantly alter the levels of any of the individual subunit mRNAs (Figure 2). A parallel cohort of control and AAS-treated animals revealed that GnRH mRNA was robustly expressed in the GFP-positive cells, but, as with the GABAA receptor subunit mRNAs, AAS treatment did not significantly alter the levels of GnRH transcripts (Figure 2).

Figure 2
AAS-dependent effects on GABAA receptor subunit and GnRH mRNA levels in GnRH neurons from control and AAS-treated mice

AAS treatment may promote post-translational modifications or alter the association of receptors with other postsynaptic components that may impose significant differences in receptor function independent of any effects on subunit composition. To further assess the effects of AAS treatment on GABAergic transmission to GnRH neurons, recordings were made of GABAA receptor-mediated sPSCs and tonic currents in identified GnRH cells. These recordings indicated that AAS treatment was without effect on the amplitude (Ipeak = 23.8 ± 1.5 pA for control and Ipeak = 24.3 ± 1.7 pA for AAS-treated) or the decay kinetics (τw = 38.9 ± 4.4 ms for control and 39.4 ± 2.9 ms for AAS-treated) of sPSCs (Figure 3A). Assessment of mPSCs also indicated that AAS treatment was without effect on either Ipeak (23.8 ± 1.7 pA for control and Ipeak = 24.9 ± 2.9 pA for AAS-treated) or τw (39.0 ± 4.1 ms for control and 40.2 ± 4.2 ms for AAS-treated). Finally, the magnitude of GABAA receptor-mediated tonic currents was also unchanged by AAS treatment: 3.83 ± 0.97 pA for control (n = 6 cells) versus 3.64 ± 0.63 pA for AAS-treated (n = 8 cells) with no differences in holding currents in either group. Taken together, these data indicate that AAS treatment was without an appreciable effect on the complement or function of postsynaptic GABAA receptors expressed in GnRH neurons.

Figure 3
AAS-dependent effects on GABAA receptor-mediated miniature and spontaneous PSCs in GnRH neurons

AAS treatment did, however, result in a significant (p = 0.014) increase in the frequency of sPSCs (0.10 ± 0.01 Hz) compared to control (0.062 ± 0.02Hz) (Figure 3B). The increase in the number of GABAA receptor-mediated synaptic events was evident only for sPSCs; AAS treatment did not alter the frequency of mPSCs (~ 0.06 Hz; Figure 3B), suggesting that AAS treatment may alter the activity, but not the number, of presynaptic GABAergic inputs to these GnRH targets.

III. AAS alters AP firing of non-GnRH mPOA neurons

The data presented above are consistent with the prevailing models that indicate that the effects of physiological steroids on GnRH neurons are likely to occur via the steroid-sensitive neurons within the basal forebrain/anterior hypothalamus that make synaptic inputs on these cells (Scott et al., 2000; Herbison, 2008). In this coronal slice orientation, neuronal networks encompassing steroid-sensitive mPOA neurons are likely candidates for the source of GABAergic afferents to GnRH neurons in the male rodent (Gao and Moore, 1996; Simerly and Swanson, 1988; Hutton et al., 1998). These neurons are also likely, due to their high level of expression of AR (Lu et al., 1998; Shah et al., 2004), to be primary targets for the direct action of 17αMTresulting in the observed changes in GABAergic tone to postsynaptic GnRH cells. Recordings were therefore also made to determine the effects of chronic exposure to 17αMT on non-GnRH neurons within the MPN of the mPOA.

Treatment of adolescent male mice with 17αMT significantly (p = 0.015) increased AP firing by these mPOA neurons from 2.6 ± 0.33 Hz for control to 3.4 ± 0.35 Hz for AAS-treated mice (Figure 4). Autocorrelational analysis indicated that AAS treatment also decreased the percentage of cells displaying irregular firing patterns and concomitantly increased the percentage displaying regular firing in mPOA neurons (Figure 4); although this trend did not attain significance (p = 0.090 for the decrease in irregular firing).

Figure 4
AAS-dependent effects on AP firing in non-GnRH mPOA neurons

IV. Disruption of inputs from mPOA neurons abrogates AAS-induced changes in presynaptic GABAergic tone and AP frequency in GnRH neurons

Data presented above suggest that long-term treatment with 17αMT increased the activity of presynaptic GABAergic mPOA neurons and thus their inhibitory drive onto GnRH neurons. In order to confirm that the AAS-mediated increase in sPSC frequency in GnRH neurons indeed arose from mPOA neuron afferents, whole-cell recordings were made from GnRH neurons that were isolated from their putative lateral presynaptic partners (e.g., ipsilateral mPOA projections) within a “disrupted mPOA” (see Methods) (Figure 5A). AP recordings from the uncut (unmanipulated) mPOA on the contralateral side of the slice were made in tandem with recordings from the disrupted side to be able to compare the effects of this surgical deafferentation in a single control or single AAS-treated animal.

Figure 5
Effects of acute interruption of inputs from the more lateral regions of the mPOA on GABAA receptor-mediated sPSC and AP firing frequencies in GnRH neurons in control and AAS-treated mice

Acute interruption of inputs from the more lateral regions of the mPOA (inclusive of the MPN) completely abrogated the AAS-induced increase in GABAA receptor-mediated sPSC frequency from a value of 0.10 ± 0.01 Hz in the intact side to 0.05 ± 0.01 Hz in the disrupted mPOA (p = 0.023; Figure 5B); a value that was not significantly different from that observed in the intact mPOA of control subjects (0.063 ± 0.02 Hz; p = 0.718). As important, disruption of the more lateral mPOA elicited mirrored changes in AP frequency in GnRH neurons: the AP frequency within the disrupted mPOA of AAS-treated mice (0.88 ± 0.15 Hz) was significantly higher (p = 0.020) than that observed in the intact mPOA (0.33 ± 0.05 Hz). Thus, as with sPSCs, acute interruption of lateral mPOA afferents restored the average AP frequency to a level not significantly different from that observed in GnRH neurons in the intact mPOA of control subjects (1.11 ± 0.17 Hz; p = 0.834) (Figure 5C). Interruption of inputs from the lateral mPOA had no effect on either peak amplitude or decay kinetics of PSCs, corroborating the conclusion noted above that the effects of AAS treatment are predominantly presynaptic. While interruption of connections from the more lateral regions of the mPOA elicited changes of comparable direction in control subjects (i.e., a decrease in sPSC frequency and a concomitant increase in AP frequency), in neither case was the effect significant (Figure 5B and C).

V. GABAergic projections from the mPOA are the critical source of increased inhibition for GnRH neuron activity in male mice under chronic AAS abuse conditions

GnRH neurons belong to a complex network comprising not only local GABAergic afferents from the mPOA, but also neurochemically heterogeneous inputs from other steroid-sensitive hypothalamic/forebrain nuclei that are likely to be retained in this coronal slice, including the anteroventral periventricular area (AVPV) (Hahn and Coen, 2006; Herbison, 2008), BNST (Simerly et al., 1990; Pompolo et al., 2002) and the suprachiasmatic nucleus (SCN) (Gu and Simerly, 1997; Van der Beek et al., 1997). Therefore, to examine the importance of GABAergic projections in mediating the actions of AAS on GnRH neuron activity, we next assessed the impact of pharmacological blockade of GABAergic transmission on AP frequency in GnRH neurons and its dependence on the physical integrity of mPOA.

On-cell recordings from GnRH neurons were made in the presence of the GABAA receptor antagonist, PTX (100 µM). Consistent with data discussed above indicating that interruption of GABAergic afferents from the more lateral mPOA did not significantly alter sPSC or AP frequency in control animals, pharmacological inhibition of GABAA receptors did not significantly alter AP frequency in either the intact or the disrupted mPOA of control subjects (Figure 6A) Conversely, in AAS-treated subjects blockade of GABAA receptors by PTX significantly increased AP frequency in GnRH neurons in the intact mPOA (p = 0.019; Figure 6B), but was without effect in the disrupted mPOA. Moreover, as predicted if the action of the AAS is to enhance inhibitory GABAergic inputs from more lateral mPOA afferents, pharmacological blockade of GABAA receptors resulted in AP firing rates in treated animals that were not statistically different in the intact versus the disrupted sides (Figure 6B).

Figure 6
Blocking GABAA receptor function enhances GnRH neuron AP firing rate only in the intact mPOA from AAS-treated male mice

Discussion

Self-administration of high doses of AAS in men and boys has been associated with changes in libido and a hypogonadal state characterized by diminished levels of serum LH and FSH, testicular atrophy, and decreased sperm production (Kam and Yarrow, 2005). Effects of exposure to AAS on pubertal onset in human subjects has not been assessed, however exposure to 17αMT decreased testes weight in adolescent male mice (McIntyre et al., 2002) and advanced vaginal opening, delayed the day of first estrus, and suppressed estrous cyclicity in adolescent female rats (Clark et al., 2006).

The mechanisms by which chronic AAS exposure disrupts the HPG axis are unknown, and may reflect both peripheral effects on gonadal tissues and central effects on neuronal circuits in the forebrain. The mPOA is a key site for the regulation of behaviors in male rodents (Hull and Dominguez, 2007), and GABAergic activity from the mPOA exerts critical control over reproductive function in males (Fernandez-Guasti et al., 1985; Seltzer and Donoso, 1992; Ojeda et al., 2006). Here we show that chronic exposure of adolescent male mice to high doses of 17αMT significantly enhanced AP firing frequency of non-GnRH neurons within the mPOA. The increase in the activity of mPOA neurons occurred in tandem with enhanced GABAA receptor-mediated sPSC frequency in putatively downstream GnRH neurons, diminished AP firing in these neuroendocrine effectors, and decreased the concentration of gonadotropins in the serum of treated mice.

Previous studies have shown that the anterorostral portion of the mPOA is composed of a dense population of GABAergic neurons (Gao and Moore, 1996; Sagrillo and Selmanoff, 1997). These GABAergic neurons within the mPOA, unlike GnRH neurons themselves, are markedly steroid-sensitive and express high levels of AR (Lu et al., 1998; Shah et al., 2004). The presence of the C17 methyl group precludes aromatization of 17αMT to17β-estradiol (Winters, 1990; Kochakian and Yesalis, 2000) suggesting that signaling by this AAS is heavily weighted to AR, versus ER. Our data indicate that the most likely mechanism by which 17αMT exerts its action on the HPG axis is via enhancement of the activity of presynaptic GABAergic neurons within the mPOA, which, in turn, promote decreased activity of downstream GnRH neurons. The mechanism by which this AAS alters presynaptic activity is not known, but may involve changes in the expression and/or function of voltage-gated conductances. An attractive candidate for such regulation may be small conductance calcium-activated potassium (SK) channels, since inhibition of these channels decreases afterhyperpolarization (AHP) and increases AP frequency in GABAergic neurons within the mPOA (Wagner et al., 2000, 2001). SK channel activity is also of critical importance in regulating the AHP and AP firing in GnRH neurons themselves (Liu and Herbison, 2008). Our data, however, demonstrate only a modest (and not significant) effect of AAS effect on AP patterning in GnRH neurons in the intact slice and abrogation of AAS-mediated effects on GnRH firing by disruption of lateral afferents. These results are most consistent with a presynaptic effect of this AAS on GABAergic afferents, rather than a direct action on the GnRH neurons themselves.

Effects of chronic steroid exposure on AP firing are likely to involve AR-mediated signaling since comparable effects were also observed in wild-type, but not AR-deficient, male mice treated with a mixture of AAS (Penatti et al., 2009b). However, alternative actions are also possible. Specifically, some of the AAS, including17αMT, can inhibit aromatase activity (de Gooyer et al., 2003; Penatti et al., 2009b) and thus may interfere with endogenous estrogenic signaling in the mPOA; signaling that provides negative feedback on GnRH neuronal activity (Pielecka and Moenter, 2006). AAS may also allosterically modulate GABAA receptors in these neurons (Henderson, 2007) and may have the potential, as yet untested, to signal through membrane AR. Estrogens have been shown to have important nongenomic actions on GnRH neuron function (Ábrahám et al., 2004; Abe and Terasawa, 2005; Ábrahám and Herbison, 2005; Abe et al., 2008; Chu et al., 2009). The nongenomic actions of estrogens in GnRH neurons are mediated via cell signaling pathways that are used by membrane AR in other cell systems (e.g., Nguyen et al., 2005), suggesting a point of potential convergence for nongenomic androgen and estrogen actions. Of particular note, estrogens have been shown to act via a nongenomic mechanism to regulate AP-independent release of GABA from presynaptic afferents to GnRH neurons (Romanó et al., 2008).

The critical role played by GABAergic neurons within the mPOA in mediating AAS effects on GnRH neuronal function is supported by experiments demonstrating that the AAS-dependent suppression of GnRH neuronal activity and AAS-dependent enhancement of GABAA receptor-mediated sPSC frequency was evident only when the mPOA circuitry lateral to the GnRH neurons was intact. Disruption of these lateral afferents restored both sPSC frequency and AP firing frequency in GnRH neurons of treated animals to levels observed in control subjects. Similarly, pharmacological antagonism of GABAA receptors in AAS-treated subjects significantly increased AP frequency in the intact mPOA, but not the disrupted mPOA. Thus, our data are consistent with the hypothesis that AAS treatment increases AP firing in GABAergic mPOA neurons, which in turn provides enhanced inhibitory tone that suppresses the firing rate of GnRH neurons. While this is the most parsimonious interpretation of our data, mPOA GABAergic afferents may also diminish GnRH neuronal firing by providing inhibitory inputs to excitatory glutamatergic or kisspeptin neurons in the AVPV or arcuate nucleus.

GnRH neurons are developmentally atypical in that GABA depolarizes these neurons well beyond early postnatal development. Whether GnRH neurons continue to be excited by GABA following puberty has prompted a lively scholarly debate (see DeFazio and Moenter, 2002; Han et al. 2002, 2004; Moenter and DeFazio, 2005; Christian and Moenter, 2007; Yin et al., 2008; Chen and Moenter, 2009), and data from dissociated adult male rat neurons suggests there may be heterogeneity within the GnRH population with respect to GABA’s action (Watanabe et al, 2009). It is also interesting to consider complexities arising from the fact that dendrites are the primary site for AP generation in GnRH neurons (Roberts et al., 2008, 2009). Thus, GABAA receptor-mediated conductance changes, while depolarizing, may nonetheless shunt more distally generated active responses and result in diminished firing frequency recorded at the soma. While further studies are required to determine whether GABA depolarizes or hyperpolarizes GnRH neurons in AAS-treated subjects, the results presented here contrast with prior studies in gonadectomized male mice indicating coordinate enhancement of GABAA receptor-mediated PSC frequency (Chen and Moenter, 2009) and AP firing (Pielecka and Moenter, 2006) in GnRH neurons. These data further underscore that actions of supraphysiological levels of synthetic steroids may significantly diverge from effects imposed by castration or by replacement in castrates with physiological androgens or estrogens.

Results presented here indicate that chronic exposure to 17αMT did not elicit significant effects on the expression of GABAA receptor subunit mRNAs, on GABAA receptor-mediated sPCS amplitude or kinetics of current decay, on tonic GABAA receptor-mediated currents, or on the level of GnRH mRNA in GnRH neurons themselves. The lack of changes in either subunit mRNAs or in properties of GABAA receptor-mediated currents suggests that the AAS do not alter the complement of GABAA receptors expressed in these cells or posttranslational modifications that would alter their function. The absence of AAS-dependent effects on these postsynaptic molecules is consistent with data demonstrating an absence of AR and a paucity of ER expression in these cells. Data demonstrating a lack of effect on GnRH mRNA levels and yet a decrease in serum gonadotropin levels are not irreconcilable as prior studies indicate a lack of correlation between these levels and GnRH message in male rodents (Gore et al., 2000; Richardson et al., 2002, 2004), except under conditions of persistent absence of steroids following gonadectomy (Spratt and Herbison, 1997; Thanky et al., 2003).

Currently ~2% of high school-age boys are estimated to have used anabolic steroids (Johnston et al., 2009). McGinnis and colleagues have shown that treatment of adolescent male rats at the onset of puberty until adulthood with a high dose (5 mg/kg) of the 17α-alkylated AAS, stanozolol, decreased testes weight, ejaculation frequency, scent marking, and vocalizations (Wesson and McGinnis, 2006). Treatment of male Syrian hamsters with a mixture of three AAS (none 17α-alkylated) augmented sexual behavior in adolescents, but suppressed it in adults (Salas-Ramirez et al., 2008), underscoring a pivotal distinction in neural templates between youth and adulthood. Gonadal steroids have been shown to have significant organizational actions on the neural circuits that give rise to male social behaviors not only during early perinatal development, but also during adolescence (Sisk et al., 2003). While our data point to central substrates through which the AAS may impair reproduction in adolescence, a key question that remains to be addressed is whether AAS exposure during this developmental period imparts permanent organizational changes on hypothalamic physiology and reproductive behaviors. Such data are particularly pertinent as studies of men (Kanayama et al., 2008) suggest that AAS users may be subject to long-term effects arising from steroid use after cessation of exposure.

Acknowledgements

This work was supported by the NIH (DA14137 to LPH). The University of Virginia Ligand Assay Core Laboratory is supported by SCCPIR U54 HD28934 from the NIH. We thank Dr. Suzanne Moenter for generously providing the original GnRH transgenic mice breeding pairs, for initial assays of serum gonadotropins and for critical review of this manuscript.

Literature cited

  • Abe H, Keen KL, Terasawa E. Rapid action of estrogens on intracellular calcium oscillations in primate luteinizing hormone-releasing hormone-1 neurons. Endocrinology. 2008;49(3) 1155-1112. [PMC free article] [PubMed]
  • Abe H, Terasawa E. Firing pattern and rapid modulation of activity by estrogen in primate luteinizing hormone releasing hormone-1 neurons. Endocrinology. 2005;146(10):4312–4320. [PMC free article] [PubMed]
  • Ábrahám IM, Herbison AE. Major sex differences in non-genomic estrogen actions on intracellular signaling in mouse brain in vivo. Neuroscience. 2005;131:945–951. [PubMed]
  • Ábrahám IM, Todman MG, Korach KS, Herbison AE. Critical in vivo Roles for Classical estrogen receptors in rapid estrogen actions on intracellular signaling in mouse brain. Endocrinology. 2004;145:3055–3061. [PubMed]
  • Bahrke MS, Yesalis CE, Kopstein AN, Stephens JA. Risk factors associated with anabolic-androgenic steroid use among adolescents. Sports Med. 2000;29:397–405. [PubMed]
  • Bar-Gad I, Ritov Y, Bergman H. The neuronal refractory period causes a short-term peak in the autocorrelation function. J Neurosci Methods. 2001;104:155–163. [PubMed]
  • Bartke A, Steele RE, Musto N, Caldwell BV. Fluctuation in plasma T levels in adult male rats and mice. Endocrinology. 1973;92:1223–1228. [PubMed]
  • Chen P, Moenter SM. GABAergic transmission to gonadotropin-releasing hormone (GnRH) neurons is regulated by GnRH in a concentration-dependent manner engaging multiple signaling pathways. J Neurosci. 2009;29:9809–9818. [PMC free article] [PubMed]
  • Christian CA, Moenter SM. Estradiol induces diurnal shifts in GABA transmission to gonadotropin-releasing hormone neurons to provide a neural signal for ovulation. J Neurosci. 2007;27:1913–1921. [PubMed]
  • Chu Z, Andrade J, Shupnik MA, Moenter SM. Differential regulation of gonadotropin-releasing hormone neuron activity and membrane properties by acutely applied estradiol: dependence on dose and estrogen receptor subtype. J Neurosci. 2009;29(17):5616–5627. [PMC free article] [PubMed]
  • Clark AS, Costine BA, Jones BL, Kelton-Rehkopf MC, Meerts SH, Nutbrown-Greene LL, Penatti CAA, Porter DM, Yang P, Henderson LP. Sex- and age-specific effects of anabolic androgenic steroids on reproductive behaviors and on GABAergic transmission in neuroendocrine control regions. Brain Res. 2006;1126:122–138. [PubMed]
  • Clark AS, Henderson LP. Behavioral and physiological responses to anabolic-androgenic steroids. Neurosci Biobehav Rev. 2003;27:413–436. [PubMed]
  • Clarkson J, Herbison AE. Development of GABA and glutamate signaling at the GnRH neuron in relation to puberty. Mol Cell Endocrinol. 2006;254–255:32–38. [PubMed]
  • Clarkson J, Herbison AE. Oestrogen, kisspeptin, GPR54 and the pre-ovulatory luteinising hormone surge. J Neuroendocrinol. 2009;21(4):305–311. [PubMed]
  • DeFazio RA, Moenter SM. Estradiol feedback alters potassium currents and firing properties of gonadotropin-releasing hormone neurons. Mol Endocrinol. 2002;16(10):2255–2265. [PubMed]
  • de Gooyer ME, Oppers-Tiemissen HM, Leysen D, Verheul HA, Kloosterboer HJ. Tibolone is not converted by human aromatase to 7α-methyl-17α-ethynylestradiol (7α-MEE): Analyses with sensitive bioassays for estrogens and androgens and with LC-MSMS. Steroids. 2003;68:235–243. [PubMed]
  • Fallest PC, Trader Gl, Darrow JM, Shupnik MA. Regulation of rat luteinizing hormone B gene expression in transgenic mice by steroids and a gonadotropin-releasing hormone antagonist. Biol Reprod. 1995;53:103–109. [PubMed]
  • Farrant M, Nusser Z. Variations on an inhibitory theme: phasic and tonic activation of GABAA receptors. Nat Rev Neurosci. 2005;6:215–229. [PubMed]
  • Fernandez-Guasti A, Larrson K, Beyer C. Comparison of the effects of different isomers of bicuculline infused in the preoptic area on male rat sexual behavior. Experientia. 1985;41:1414–1416. [PubMed]
  • Franklin KBJ, Paxinos G. The Mouse Brain in Stereotaxic Coordinates. San Diego CA: Academic Press; 1997.
  • Gao B, Moore RY. The sexually dimorphic nucleus of the hypothalamus contains GABA neurons in rat and man. Brain Res. 1996;742:163–171. [PubMed]
  • Gay VL, Midgley AR, Jr, Niswender GD. Patterns of gonadotropin secretion associated with ovulation. Fed Proc. 1970;29:1880–1887. [PubMed]
  • Gore AC, Wersinger SR, Rissman EF. Effects of female pheromones on gonadotropin-releasing hormone gene expression and luteinizing hormone release in male wild-type and oestrogen receptor-alpha knockout mice. J Neuroendocrinol. 2000;12(12):1200–1204. [PubMed]
  • Grattan DR, Jasoni CL, Liu X, Anderson GM, Herbison AE. Prolactin regulation of gonadotropin-releasing hormone neurons to suppress luteinizing hormone secretion in mice. Endocrinology. 2007;148(9):4344–4351. [PubMed]
  • Grattan DR, Rocca MS, Sagrillo CA, McCarthy MM, Selmanoff M. Antiandrogen microimplants into the rostral medial preoptic area decrease γ-aminobutyric acidergic neuronal activity and increase luteinizing hormone secretion in the intact male. Endocrinology. 1996;137:4167–4173. [PubMed]
  • Gu GB, Simerly RB. Projections of the sexually dimorphic anteroventral periventricular nucleus in the female rat. J Comp Neurol. 1997;384(1):142–164. [PubMed]
  • Hahn JD, Coen CW. Comparative study of the sources of neuronal projections to the site of gonadotrophin-releasing hormone perikarya and to the anteroventral periventricular nucleus in female rats. J Comp Neurol. 2006;494:190–214. [PubMed]
  • Hájos N, Nusser Z, Rancz EA, Freund TF, Mody I. Cell type- and synapse-specific variability in synaptic GABAA receptor occupancy. Eur J Neurosci. 2000;12(3):810–818. [PubMed]
  • Han SK, Abraham IM, Herbison AE. Effect of GABA on GnRH neurons switches from depolarization to hyperpolarization at puberty in the female mouse. Endocrinology. 2002;143:1459–1466. [PubMed]
  • Han SK, Todman MG, Herbison AE. Endogenous GABA release inhibits the firing of adult gonadotropin-releasing hormone neurons. Endocrinology. 2004;145:495–499. [PubMed]
  • Henderson LP. Steroid modulation of GABAA receptor-mediated transmission in the hypothalamus: Effects on reproductive function. Neuropharmacology. 2007;52:1439–1453. [PMC free article] [PubMed]
  • Herbison AE. Estrogen positive feedback to gonadotropin-releasing hormone (GnRH) neurons in the rodent: The case for the rostral periventricular area of the third ventricle (RP3V) Brain Res Rev. 2008;57(2):277–287. [PubMed]
  • Hrabovszky E, Steinhauser A, Barabás K, Shughrue PJ, Petersen SL, Merchenthaler I, Liposits Z. Estrogen receptor-β immunoreactivity in luteinizing hormone-releasing hormone neurons of the rat brain. Endocrinology. 2001;142:3261–3264. [PubMed]
  • Hu L, Gustofson RL, Feng H, Leung PK, Mores N, Krsmanovic LZ, Catt KJ. Converse regulatory functions of estrogen receptor-alpha and -beta subtypes expressed in hypothalamic gonadotropin-releasing hormone neurons. Mol Endocrinol. 2008;22(10):2250–2259. [PubMed]
  • Hull EM, Dominguez JM. Sexual behavior in male rodents. Horm Behav. 2007;52(1):45–55. [PMC free article] [PubMed]
  • Hutton LA, Gu G, Simerly RB. Development of a sexually dimorphic projection from the bed nucleus of the stria terminalis to the anteroventral periventricular nucleus in the rat. J Neurosci. 1998;18:3003–3013. [PubMed]
  • Irving LM, Wall M, Neumark-Sztainer D, Story M. Steroid use among adolescents: Findings from Project EAT. J Adol Health. 2002;30:243–252. [PubMed]
  • Johnston LD, O'Malley PM, Bachman JG, Schulenberg JE. Bethesda, MD: National Institute on Drug Abuse; 2009. Monitoring the Future national results on adolescent drug use: Overview of key findings, 2008 (NIH Publication No. 09–7401) p. 73.
  • Jones BL, Whiting PJ, Henderson LP. Mechanisms of anabolic androgenic steroid inhibition of mammalian ε-subunit-containing GABAA receptors. J Physiol (Lond) 2006;573.3:571–593. [PubMed]
  • Kam PCA, Yarrow M. Anabolic steroid abuse: physiological and anaesthetic considerations. Anaesthesia. 2005;60:685–692. [PubMed]
  • Kanayama G, Hudson JI, Pope HG., Jr Long-term psychiatric and medical consequences of anabolic-androgenic steroid abuse: a looming public health concern? Drug Alcohol Depend. 2008;98(1–2):1–12. [PMC free article] [PubMed]
  • Keene DE, Suescun MO, Bostwick MG, Chandrashekar V, Bartke A, Kopchick JJ. Puberty is delayed in male growth hormone receptor gene-disrupted mice. J Androl. 2002;23(5):661–668. [PubMed]
  • Kelly MJ, Wagner EJ. GnRH neurons and episodic bursting activity. Trends Endocrinol Metab. 2002;13(10):409–410. [PubMed]
  • Kochakian C, Yesalis CE. Anabolic-androgenic steroids: a historical perspective and definition. In: Yesalis CE, editor. Anabolic Steroids in Sport and Exercise. Champaign: Human Kinetics; 2000. pp. 4–33.
  • Kudwa AE, Gustafsson J-Å, Rissman EF. Estrogen receptor β modulates estradiol induction of progestin receptor immunoreactivity in male, but not in female, mouse medial preoptic area. Endocrinology. 2004;145:4500–4506. [PubMed]
  • Li J, Smyth P, Cahill S, Denning K, Flavin R, Aherne S, Pirotta M, Guenther SM, O'Leary JJ, Sheils O. Improved RNA quality and TaqMan Pre-amplification method (PreAmp) to enhance expression analysis from formalin fixed paraffin embedded (FFPE) materials. BMC Biotechnol. 2008;8(10):1–11. [PMC free article] [PubMed]
  • Liu X, Herbison AE. Small-conductance calcium-activated potassium channels control excitability and firing dynamics in gonadotropin-releasing hormone (GnRH) neurons. Endocrinology. 2008;149:3598–3604. [PubMed]
  • Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT Method. Methods. 2001;25:402–408. [PubMed]
  • Lu S-F, McKenna SE, Cologer-Clifford A, Nau EA, Simon NG. Androgen receptor in mouse brain: sex differences and similarities in autoregulation. Endocrinology. 1998;139:1594–1601. [PubMed]
  • McIntyre KL, Porter DM, Henderson LP. Anabolic androgenic steroids induce age-, sex-, and dose dependent changes in GABAA receptor subunit mRNAs in the mouse forebrain. Neuropharmacology. 2002;43:634–645. [PubMed]
  • Mitra SW, Hoskin E, Yudkovitz J, Pear L, Wilkinson HA, Hayashi S, Pfaff DW, Ogawa S, Rohrer SP, Schaeffer JM, McEwen BS, Alves SE. Immunolocalization of estrogen receptor β in the mouse brain: comparison with estrogen receptor α Endocrinology. 2003;144:2055–2067. [PubMed]
  • Mitsushima D, Funabashi T, Kimura F. Fos expression in gonadotropin-releasing hormone neurons by naloxone or bicuculline in intact male rats. Brain Res. 1999;839(1):209–212. [PubMed]
  • Mitsushima D, Tin-Tin-Win-Shwe, Kimura F. Sexual dimorphism in the GABAergic control of gonadotropin release in intact rats. Neurosci Res. 2003;46(4):399–405. [PubMed]
  • Moenter SM, DeFazio RA. Endogenous γ-aminobutyric acid can excite gonadotropin-releasing hormone neurons. Endocrinology. 2005;146:5374–5379. [PubMed]
  • Moenter SM, DeFazio RA, Pitts GR, Nunemaker CS. Mechanisms underlying episodic gonadotropin-releasing hormone secretion. Front Neuroendocrinol. 2003;24:79–93. [PubMed]
  • Moragues N, Cioffi P, Lafon P, Tramu G, Garret M. GABAA receptor ε-subunit expression in identified peptidergic neurons of the rat hypothalamus. Brain Res. 2003;967:285–289. [PubMed]
  • Nguyen T-VV, Yao M, Pike CJ. Androgens activate mitogen-activated protein kinase signaling: Role in neuroprotection. J Neurochem. 2005;94:1639–1651. [PubMed]
  • Nomura M, Korach KS, Pfaff DW, Ogawa S. Estrogen receptor β (ERβ) protein levels in neurons depend on estrogen receptor α (ERα) gene expression and on its ligand in a brain region-specific manner. Brain Res Mol Brain Res. 2003;110:7–14. [PubMed]
  • Nusser Z, Cull-Candy S, Farrant M. Differences in synaptic GABAA receptor number underlie variation in GABA mini amplitude. Neuron. 1997;19(3):697–709. [PubMed]
  • Ojeda SR, Lomniczi A, Mastronardi C, Heger S, Roth C, Parent A-S, Matagne V, Mungenast AE. Minireview: The neuroendocrine regulation of puberty: Is the time ripe for a systems biology approach? Endocrinology. 2006;147:1166–1174. [PubMed]
  • Pape JR, Skynner MJ, Sim JA, Herbison AE. Profiling γ-aminobutyric acid (GABAA) receptor subunit mRNA expression in postnatal gonadotropin-releasing hormone (GnRH) neurons of the male mouse with single cell RT-PCR. Neuroendocrinology. 2001;74:300–308. [PubMed]
  • Peirson SN, Butler JN, Foster RG. Experimental validation of novel and conventional approaches to quantitative real-time PCR data analysis. Nucleic Acids Res. 2003;31(14):e73. [PMC free article] [PubMed]
  • Penatti CAA, Costine BA, Porter DM, Henderson LP. Effects of chronic exposure to an anabolic androgenic steroid cocktail on α5-receptor mediated GABAergic transmission and neural signaling in the forebrain of female mice. Neuroscience. 2009a;161:526–537. [PMC free article] [PubMed]
  • Penatti CAA, Porter DM, Henderson LP. Chronic exposure to anabolic androgenic steroids alters neuronal function in the mammalian forebrain via androgen receptor- and estrogen receptor-mediated mechanisms. J Neurosci. 2009b;29(40):12484–12496. [PMC free article] [PubMed]
  • Penatti CAA, Porter DM, Jones BL, Henderson LP. Sex-specific effects of chronic anabolic androgenic steroid treatment on GABAA receptor expression and function in adolescent mice. Neuroscience. 2005;135:533–543. [PubMed]
  • Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001;29(9):e45. 2001. [PMC free article] [PubMed]
  • Pfaffl MW, Horgan GW, Dempfle L. Relative expression software tool (REST©) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res. 2002;30(9):e36. [PMC free article] [PubMed]
  • Pielecka J, Moenter SMM. Effect of steroid milieu on gonadotropin-releasing hormone-1 neuron firing pattern and luteinizing hormone levels in male mice. Biol Reprod. 2006;74:931–937. [PubMed]
  • Pierroz DD, Aebi AC, Huhtaniemi IT, Aubert ML. Many LH peaks are needed to physiologically stimulate testosterone secretion: modulation by fasting and NPY. Am J Physiol Endocrinol Metab. 1999;276:603–610. [PubMed]
  • Pompolo S, Scott CJ, Clarke IJ. Selective regulation of glutamic decarboxylase isoform 65, but not isoform 67, in the bed nucleus of the stria terminalis and the preoptic area of the ewe brain across the estrous cycle. Endocrinology. 2002;143:544–550. [PubMed]
  • Richardson HN, Gore AC, Venier J, Romeo RD, Sisk CL. Increased expression of forebrain GnRH mRNA and changes in testosterone negative feedback following pubertal maturation. Mol Cell Endocrinol. 2004;214(1–2):63–70. [PubMed]
  • Richardson HN, Parfitt DB, Thompson RC, Sisk CL. Redefining gonadotropin-releasing hormone (GnRH) cell groups in the male Syrian hamster: testosterone regulates GnRH mRNA in the tenia tecta. J Neuroendocrinol. 2002;(5):375–383. [PubMed]
  • Roberts CB, Campbell RE, Herbison AE, Suter KJ. Dendritic action potential initiation in hypothalamic gonadotropin-releasing hormone neurons. Endocrinology. 2008;149:3355–3360. [PubMed]
  • Roberts CB, O’Boyle MP, Suter KJ. Dendrites determine the contribution of after depolarization potentials (ADPs) to generation of repetitive action potentials in hypothalamic gonadotropin releasing-hormone (GnRH) neurons. J Comput Neurosci. 2009;26:39–53. [PubMed]
  • Romanó N, Lee K, Ábrahám IM, Jasoni C, Herbison AE. Nonclassical estrogen modulation of presynaptic GABA terminals modulates calcium dynamics in gonadotropin-releasing hormone neurons. Endocrinology. 2008;149:5335–5344. [PubMed]
  • Rosweir AK, Kauffman AS, Smith JT, Guerriero KA, Morgan K, Pielecka-Fortuna J, Pineda R, Gottsch ML, Tena-Sempere M, Moenter SM, Terasawa E, Clarke IJ, Steiner RA, Millar RA. Discovery of potent kisspeptin antagonists delineate physiological mechanisms of gonadotropin regulation. J Neurosci. 2009;29:3920–3929. [PMC free article] [PubMed]
  • Sagrillo CA, Selmanoff M. Castration decreases single cell levels of mRNA encoding glutamic acid decarboxylase in the diagonal band of Broca and the sexually dimorphic nucleus of the preoptic area. J Neuroendocrinol. 1997;9:699–706. [PubMed]
  • Salas-Ramirez KY, Montalto PR, Sisk CL. Anabolic androgenic steroids differentially affect social behaviors in adolescent and adult male Syrian hamsters. Horm Behav. 2008;53:378–385. [PMC free article] [PubMed]
  • Sato SM, Schulz KM, Sisk CL, Wood RI. Adolescents and androgens, receptors and rewards. Horm Behav. 2008;53:647–658. [PMC free article] [PubMed]
  • Scott CJ, Tilbrook AJ, Rawson JA, Clarke IJ. Gonadal steroid receptors in the regulation of GnRH secretion in farm animals. Anim Reprod Sci. 2000;60–61:313–326. [PubMed]
  • Selmanoff KM, Goldman BD, Ginsburg BE. Developmental changes in serum luteinizing hormone, follicle stimulating hormone and androgen levels in males of two inbred mouse strains. Endocrinology. 1977;100:122–127. [PubMed]
  • Seltzer AM, Donoso AO. Restraining action of GABA on estradiol-induced LH surge in the rat: GABA activity in brain nuclei and effects of GABA mimetics in the medial preoptic nucleus. Neuroendocrinology. 1992;55(1):28–34. [PubMed]
  • Shah NM, Pisapia DJ, Maniatis S, Mendelsohn MM, Nemes A, Axel R. Visualizing sexual dimorphism in the brain. Neuron. 2004;43:313–319. [PubMed]
  • Sim JA, Skynner MJ, Pape JR, Herbison AE. Late postnatal reorganization of GABAA receptor signalling in native GnRH neurons. Eur J Neurosci. 2000;12:3497–3504. [PubMed]
  • Simerly RB, Chang C, Muramatsu M, Swanson LW. Distribution of androgen and estrogen receptor mRNA-containing cells in the rat brain: an in situ hybridization study. J Comp Neurol. 1990;294:76–95. [PubMed]
  • Simerly RB, Swanson LW. Projections of the medial preoptic nucleus: a Phaseolus vulgaris leucoagglutinin anterograde tract-tracing study in the rat. J Comp Neurol. 1988;270:209–242. [PubMed]
  • Sisk CL, Schulz KM, Zehr JL. Puberty: a finishing school for male social behavior. Ann N Y Acad Sci. 2003;1007:189–198. [PubMed]
  • Spratt DP, Herbison AE. Regulation of preoptic area gonadotrophin-releasing hormone (GnRH) mRNA expression by gonadal steroids in the long-term gonadectomized male rat. Brain Res Mol Brain Res. 1997;47(1–2):125–133. [PubMed]
  • Steiner RA, Bremner WJ, Clifton DK. Regulation of luteinizing hormone pulse frequency and amplitude by T in the adult male rat. Endocrinology. 1982;111:2055–2061. [PubMed]
  • Suter KJ, Song WJ, Sampson TL, Wuarin JP, Saunders JT, Dudek FE, Moenter SM. Genetic targeting of green fluorescent protein to gonadotropin-releasing hormone neurons: characterization of whole-cell electrophysiological properties and morphology. Endocrinology. 2000;141(1):412–419. [PubMed]
  • Thanky NR, Slater R, Herbison AE. Sex differences in estrogen-dependent transcription of gonadotropin-releasing hormone (GnRH) gene revealed in GnRH transgenic mice. Endocrinology. 2003;144(8):3351–3358. [PubMed]
  • Tin-Tin-Win-Shwe, Mitsushima D, Kimura F. Profiles of in vivo gamma-aminobutyric acid release in the medial preoptic area of intact and castrated male rats. Neuroendocrinology. 2002;76(5):290–296. [PubMed]
  • Tin-Tin-Win-Shwe, Mitsushima D, Shinohara K, Kimura F. Sexual dimorphism of GABA release in the medial preoptic area and luteinizing hormone release in gonadectomized estrogen-primed rats. Neuroscience. 2004;127(1):243–250. [PubMed]
  • Todman MG, Han SK, Herbison AE. Profiling neurotransmitter receptor expression in mouse gonadotropin-releasing hormone neurons using green fluorescent protein-promoter transgenics and microarrays. Neuroscience. 2005;132(3):703–712. [PubMed]
  • Turi GF, Liposits Z, Moenter SM, Fekete C, Hrabovszky E. Origin of Neuropeptide Y-containing afferents to gonadotropin-releasing hormone neurons in male mice. Endocrinology. 2003;144:4967–4974. [PubMed]
  • Van der Beek EM, Horvath TL, Wiegant VM, Van den Hurk R, Buijs RM. Evidence for a direct neuronal pathway from the suprachiasmatic nucleus to the gonadotropin-releasing hormone system: combined tracing and light and electron microscopic immunocytochemical studies. J Comp Neurol. 1997;384(4):569–579. [PubMed]
  • Wagner EJ, Reyes-Vazquez C, Rønnekleiv OK, Kelly MJ. the role of intrinsic and agonist-activated conductances in determining the firing patterns of preoptic area neurons in the guinea pig. Brain Res. 2000;879:29–41. [PubMed]
  • Wagner EJ, Rønnekleiv OK, Kelly MJ. The noradgrenergic inhibition of an apamine-sensitive small conductance Ca2+-activated K+ channel in hypothalamic γ-aminobutyric acid neurons: pharmacology, estrogen sensitivity and relevance to the control of the reproductive axis. J Pharmacol Exp Ther. 2001;299:21–30. [PubMed]
  • Watanabe M, Sakuma Y, Kato M. GABAA receptors mediate excitation in adult rat GnRH neurons. Biol Reprod. 2009;81:327–332. [PubMed]
  • Wesson DW, McGinnis MY. Stacking anabolic androgenic steroids (AAS) during puberty in rats: A neuroendocrine and behavioral assessment. Pharmacol Biochem Behav. 2006;83:410–419. [PubMed]
  • Winters SJ. Androgens: endocrine physiology and pharmacology. NIDA Res Monogr. 1990;102:113–130. [PubMed]
  • Yang P, Jones B, Henderson LP. Mechanisms of anabolic androgenic steroid modulation of α1β3γ2L GABAA receptors. Neuropharmacology. 2002;43:619–633. [PubMed]
  • Yin C, Ishii H, Tanaka N, Sakuma Y, Kato M. Activation of A-type γ-aminobutyric acid receptors excites gonadotrophin-releasing hormone neurones isolated from adult rats. J Neuroendocrinol. 2008;20:566–575. [PubMed]