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We examined the effects of chronic metals ingestion on social behavior in the normally highly social prairie vole to test the hypothesis that metals may interact with central dopamine systems to produce the social withdrawal characteristic of autism. Relative to water-treated controls, ten weeks of chronic ingestion of either Hg++ or Cd++ via drinking water significantly reduced social contact by male voles when they were given a choice between isolation or contact with an unfamiliar same-sex conspecific. The effects of metals ingestion were specific to males: no effects of metals exposure were seen in females. Metals ingestion did not alter behavior of males allowed to choose between isolation or their familiar cagemates, rather than strangers. We also examined the possibility that metals ingestion affects central dopamine functioning by testing the voles’ locomotor responses to peripheral administration of amphetamine. As with the social behavior, we found a sex-specific effect of metals on amphetamine responses. Males that consumed Hg++ did not increase their locomotor activity in response to amphetamine, whereas similarly-treated females and males that ingested only water significantly increased their locomotor activities. Thus, an ecologically relevant stimulus, metals ingestion, produced two of the hallmark characteristics of autism – social avoidance and a male-oriented bias. These results suggest that metals exposure may contribute to the development of autism, possibly by interacting with central dopamine function, and support the use of prairie voles as a model organism in which to study autism.
The autism spectrum disorders are widespread in the developed world and the incidence of autism may be increasing. It is clear from several decades of study that autism is a complex (of) disorder(s) involving both genetic and environmental factors, but there is far from consensus as to the relative influence of each, or even if the same factors affect all autism sufferers in the same fashion [23, 27, 55, 58, 69]. Significant effort has been expended toward identifying genetic underpinnings of autism. However, observations such as the lack of total concordance of autism in monozygotic twins , suggest that genetic causes cannot account for all aspects of autism. Thus, it may be just as important to identify environmental triggers as it is to identify genetic vulnerabilities (see also ).
One reason for the slow progress in gaining an understanding of autism is the relative lack of ecologically relevant animal models in which to study potential causes of the disorder (but see [73, 74, 78]). Any animal model that is intended as a platform on which to base studies of autism must, at minimum, address the broad aspects and symptoms of autism: social dysfunction, restricted or repetitive behaviors and/or perseveration, language impairments, and the fact that sex differences are apparent in the incidence of autism [76, 87, 91]. Unfortunately, many of the current animal models are limited in their ability to address the broader aspects of autism . Consequently, rather than modeling autism as a disorder, many animal studies focus on specific symptoms of autism. For example, lesion studies typically are targeted toward brain regions that may logically be implicated in autism. When successful, the brain lesions produce behaviors characteristic of autism, and thereby provide important evidence supporting involvement of specific brain regions in autism [7, 21]. However, such techniques do not model the disorder itself, which makes determination of causal factors and effective treatments problematic. As an alternative to the currently used animal models, we propose a unique animal model in which to study autism and a novel mechanism by which autism may be manifested.
Although there often are cognitive deficits associated with the disorder, at its core autism may best be described as a socialization disorder, and the social impairments may persist throughout life. In a comparison of autism symptoms in adults and adolescents, Seltzer et al.  found that deficits in social behaviors were least likely to improve with age. Thus, examining social behavior may be the most direct route to understanding autism. For the past two decades, prairie voles (Microtus ochrogaster) have been the dominant animal model in which to study the formation and maintenance of social affiliations [6, 14] and, more recently, have gained the attention of researchers interested in autism [44, 93]. In contrast to more traditional laboratory animals, prairie voles display social behaviors remarkably similar to those of humans, even displaying characteristics of monogamy such as long-term pair-bonding, care of offspring by both parents, and sharing of a nest even beyond the breeding season . Further, autonomic responses in voles are more similar to those of humans than they are to other rodent species . These parallels have led to the extensive use of voles to study quantitatively the behavioral, neural, and physiological bases of social attachment [5, 16, 20, 24, 29, 36, 38, 39, 45, 52, 88, 89]. As a result, both the behavioral repertoire and the physiology of voles are well documented, so there is a strong literature base upon which future studies can rest. Moreover, voles likely possess much greater genetic variability than do the inbred strains of rats and mice typically used as rodent models. For a disorder such as autism that may have multiple genetic vulnerabilities, an animal model with more genetic variability may confer significant advantages, including more closely paralleling the genetic variability of humans and serving to reduce the possibility of false negatives.
A number of studies have implicated exposure to various metals in the development of autism [1, 26, 32, 62, 71, 90] but, to date, mechanisms by which metals exposure may cause autism have been difficult to ascertain. Recently, two independent lines of research have converged to provide a possible link between metals exposure and autism. First, there is evidence that low-level exposure to metals (mercury, lead, copper and other multivalent cations) can alter dopamine transporter (DAT) functioning [51, 54] including that in cells derived from the ventral striatum . In vivo, one outcome of altered DAT functioning could be altered synaptic dopamine concentrations, which may, in turn, result in abnormal dopamine receptor activation patterns in people with metals exposure. A second line of research has shown that changes in patterns of dopamine receptor activation in the ventral striatum can fundamentally alter the behavioral responses of prairie voles , such that exposure to a novel individual produces an aversive response rather than an affiliative response during social interactions . These findings have led us to hypothesize that changes in dopamine functioning due to metals exposure may contribute substantially to the symptoms associated with autism. As a first test of this hypothesis, we provided sexually naïve adult prairie voles of both sexes with solutions containing metals as their sole drinking water sources, while a second group of voles was treated identically except that they received unadulterated de-ionized water to drink. After several weeks of metals consumption, group comparisons of a variety of social and non-social measures were performed.
Animals were housed in USDA approved facilities with general animal care provided by Laboratory Animal Resources personnel. Animals were monitored daily and veterinary staff was available for consultation regarding animal health and welfare. All experimental manipulations, animal handling procedures, and behavioral testing were approved by the Oklahoma State University Center for Health Sciences Institutional Animal Care and Use Committee.
Subjects were sexually-naïve adult prairie voles of both sexes from a laboratory breeding-colony descended from an Illinois population and were of the F3 and F4 generations relative to the most recent out-crossing with wild stock. Breeding pairs (F2 and F3 generations) were housed in plastic cages (20×25×45 cm) containing corncob bedding with hay as nesting material. Ad libitum food (rabbit chow supplemented with sunflower seeds) and water were available. Offspring were weaned at 19–21 days of age and were housed in same-sex pairs in plastic cages (10×17×28 cm) maintained at 21°C with a 14:10 light:dark cycle. After weaning, males were maintained in a separate room from females and the breeder stock until used in experiments. However, during experimental manipulations, subjects and control animals of both sexes were housed in a single room.
Same-sex pairs were randomly assigned to treatment groups that received dilute (60 ppm) mercuric chloride (HgCl2) or cadmium chloride (CdCl2) solutions as their sole drinking water sources. These concentrations are near the low end of the range of concentrations found during a survey of the toxicology literature. Control pairs received unadulterated drinking water. Groups otherwise were treated identically. Fresh solutions were supplied as part of normal cage maintenance, although solutions were replaced if they became contaminated with bedding outside of the normal cage maintenance regimen. Fresh metals solutions were prepared weekly. In sub-groups of animals of each sex, water and metal ingestion by sibling pairs were monitored by weighing bottles each time solutions were changed. Since animals required multiple housing, ingestion was assumed to be equally distributed between the two individuals in each same-sex pair. For most animals, behavioral testing took place around the tenth week of exposure. A separate group of males was subjected to behavioral testing after about 4 weeks of Hg ingestion to examine whether treatment effects would be present after shorter metals exposure.
Social interactions were examined using an apparatus consisting of two parallel cages (10×17×28 cm) connected by a tube (7.5×16 cm). One cage was always empty and, depending on the goal of the test, the other cage contained either an unfamiliar vole of the same sex and of similar age and size as the subject, or the subject’s familiar same-sex cage-mate, as a stimulus animal. The stimulus animals were tethered loosely to confine them to one cage while the subject had unfettered access to both cages. Subjects were released into the empty cage and their behavior was monitored for 3 hours. Movements between cages were counted using a customized computer program (R. Henderson, Florida State University) that monitored light-beams across the tube connecting the cages. All social interaction testing was digitally recorded for later detailed analysis of social behavior. The primary dependent measure was time spent by the subject in quiet direct contact (with or without auto- or allo-grooming) with the stimulus animal. The number of crossings between cages was used as an index of locomotor activity. In two cases, unusual aggression on the part of the stimulus animal necessitated excluding subjects from the final behavioral analysis.
Statistical analyses consisted of two-way ANOVA (sex × metal) to examine sex effects of metals exposure, or two-way ANOVA (stimulus animal × metal) when males were tested for responses toward a stranger vs. toward a familiar partner. Significant (p < 0.05) main effects or interactions were examined further using Student-Neuman-Keuls (SNK) analyses.
Additional groups of 6–8 animals of each sex were assigned to groups that received either untreated drinking water or drinking water containing 60 ppm Hg++. After 10 weeks, locomotor behaviors by these animals in response to amphetamine were tested. Locomotor activity was measured twice for each animal, once after being injected with a 0.5mg/kg dose of d-amphetamine (in 0.1 ml/40g of body weight of isotonic saline vehicle), and once after receiving an equivalent volume of the saline vehicle alone. Testing occurred on two consecutive days with the order of treatments counter-balanced. Immediately after being injected, each vole was placed into an open-field arena (floor area 43 × 43 cm, Noldus) for ten minutes and locomotor behavior was digitally recorded using a video camera mounted approximately 2 m directly overhead. The total distance traveled by each animal during the test was quantified using Noldus EthoVision XT6. The performance of each animal was defined as the difference between the distances traveled after the vehicle and amphetamine injections.
After behavioral testing, most voles were weighed and sacrificed; the brains were harvested and divided into several portions and stored at −80°C until used for tissue analyses.
A subsample of brainstems (n = 6/treatment/sex) was assayed for tissue mercury concentration at Texas Tech University, Lubbock, Texas, using established procedures . Each brainstem was digested with HNO3 at elevated temperatures, treated with peroxide, and diluted to 10 ml with de-ionized water, as described in EPA protocol 3050B. Digested samples then were analyzed using a cold vapor mercury procedure. Solutions of known concentration were used to create standard curves. No mercury was detected in blanks and recovery from spiked samples was between 85 and 106%. The detection limit was 0.04 mg/kg of tissue. All control samples were below reliable detection limits for tissue Hg++ content. For these samples, the actual values generated in the analyses were used for statistical purposes, but the results are reported as below detection limits.
All animals were at least 59 days of age at the onset of any treatments or testing. At the time of the behavioral testing, the mean age of animals that received ten week metals exposure was 147.9 ± 3.5 days (n = 86) and there were no sex differences in age at testing (F1,85 = 1.85, p = 0.18; males – 144.4 ± 3.7 days, females - 154.0 ± 6.8 days). There also were no differences in the duration of metals exposure for sex (F1,42 = 1.76, p = 0.19; males – 72 ± 1.4 days, females - 75 ± 1.6 days) or for metal (F1,42 = 1.84, p = 0.18; Hg++ - 71 ± 1.7 days, Cd++ – 76 ± 0.4 days). Males tested after shorter metals exposure received Hg++ in their drinking water for 30.7 ± 1.1 days prior to testing. Due to the shorter treatment duration, males in this experiment were tested at an earlier age (101.5 ± 1.4 days of age).
Groups that received only water and groups that received water containing Hg++ or Cd++ did not differ in the total amount of liquid ingested during the first week of exposure (Fig 1). Total liquid ingestion by one group of animals that received Hg++ was monitored for the entire 10 week exposure period. There were no sex differences (n = 6 pairs of each sex) in total amount of mercury ingested, either in terms of absolute amounts ingested (F1,11 = 1.66, p = 0.22; males – 41.3 ± 3.1 mg, females - 50.8 ± 7.7 mg) or when intakes were expressed as amount per unit body weight (F1,11 = 2.51, p = 0.14; males - 1.1 ± 0.1 mg/g of body weight, females – 1.4 ± 0.2 mg/g of body weight). There were significant sex differences in body weight at termination (F1,52 = 16.91, p < 0.001), with males being somewhat heavier than females (males- 42.9 ± 1.1 g, females – 38.0 ± 0.8 g). In addition, Cd++-treated males were heavier (47.5 ± 1.3 g) than either Hg++-treated (40.5 ± 1.8 g, p < 0.004) or control males (41.5 ± 1.5 g, p < 0.02). There were higher levels of Hg++ in the brains of animals that received Hg++ (0.39 ± 0.09 μg/g tissue) than in the animals that consumed only water (< 0.04 μg/g tissue; F2,20 = 17.42, p < 0.001). When brain tissue from only those animals that received Hg++ was compared, there were no sex differences (F1,10 = 0.23, p = 0.64; males – 0.34 ± 0.05 μg/g of tissue, females – 0.43 ± 0.10 μg/g of tissue).
When the amount of time that the subjects (n = 10/group) spent engaged in quiet, direct contact with an unfamiliar stranger was compared, a strong sex by treatment interaction was found (F1,36 = 10.53, p < 0.01). Male voles that consumed water containing Hg++ or Cd++ spent half as much time, or less, in contact with the stranger compared to control males (Fig 2; H2O-treated v. Hg++ -treated, p < 0.002; H2O-treated v. Cd-treated, p < 0.001), while contact by Hg++- and Cd++-treated males did not differ (p = 0.09). In contrast to the effects seen in males, female voles that ingested metals in their drinking water did not differ from control females in the amount of time spent in direct contact with a stranger (Fig 2; H2O-treated v. Hg++ -treated, p = 0.60; H2O-treated v. Cd-treated, p = 0.15).
Males that consumed metals in their drinking water were given the choice between an empty cage and a cage containing either their familiar cage-mates or unfamiliar strangers (Fig 3). There were significant effects of metal exposure (F2,49 = 5.53, p < 0.01) and the type of stimulus animal used in the choice test (F1,49 = 16.64, p < 0.001). Males that consumed only water did not differ in the amounts of time spent with either a stranger or their familiar cage-mates. In contrast, males that ingested Hg++ or Cd++ in their drinking water avoided strangers, but not their familiar cage-mates.
There was a significant treatment effect for males tested after 4 weeks of Hg++ exposure (F1,35 = 11.9, p < 0.002). As was seen with the longer exposure time, males that consumed Hg++ for 4 weeks spent less time in contact with the unfamiliar stimulus animal (Fig. 4) than did control males.
There were no effects of sex (F1,60 = 0.41, p = 0.53) or metals consumption (F2,60 = 0.49, p = 0.62) on locomotor activity during the exposure to the stranger (Fig 5). Similarly, there were no effects of metal treatment (F1,38 = 0.15, p = 0.70) or stimulus animal type (F1,38 = 1.10, p = 0.30) on locomotor activity when males were tested for responses to a stranger v. to a familiar cage-mate (Fig 5).
A separate group of Hg++-treated voles was tested for locomotor responses to amphetamine administration. There was a significant interaction between sex and treatment (F1,22 = 4.66, p < 0.05). Male voles that received Hg++ (Fig 6) displayed significantly blunted responses relative to similarly treated females (p < 0.02) or to males that received only water (p < 0.02). Hg++ treatment had no effect on amphetamine responses in females (H2O v. Hg++, p = 0.76). Counter-balancing the order of injections did not affect the animals’ responses as there were no significant differences in locomotor activity after saline injections on the first or second days of treatment (F1,24 = 1.21, p = 0.28).
We have used ecologically relevant stimuli in a novel animal model to produce changes in ethologically valid measures of behavior that parallel two of the defining characteristics of autism. Our primary finding is that chronic ingestion of metals by a normally very social animal, the prairie vole, produces decreases in social contact which may parallel the social deficits observed in autistic patients. Further, we found that the effects of metals ingestion on social behavior are confined to males, which may parallel the male-bias characteristic of autism. In addition, we found that metals ingestion also alters a non-social behavior that is widely known to be dopamine-mediated, increased locomotor activity after amphetamine administration, and this change also was restricted to males. To our knowledge, this is the first use of prairie voles to test specific hypotheses regarding the processes underlying autism.
A variety of studies have provided evidence, either direct or indirect, that central dopamine plays a role in autism. For example, dopamine is involved in many of the behavioral and cognitive functions that are impaired in autistic patients [8, 25, 33, 40, 50] and in many cases, the effects of dopamine manipulations are sex-specific [2, 19, 60, 66]. In addition, drugs that target central dopamine systems can have significant effects (positive or negative) on autism symptoms [22, 40, 50]. Further, the stereotyped dyskinesias associated with autism have been shown to derive from hyperdopaminergic activity rather than from decreases in opposing neurochemical actions[8, 33]. Finally, polymorphisms of several proteins involved in dopamine metabolism and in dopamine receptor genes have been correlated with various aspects of autism [30, 41, 49], and knockout mice that lack the dopamine transporter are more likely to display aversive responses in a social encounter . Thus, genetic differences in dopamine systems could contribute to the variability in susceptibility to autism, particularly in the face of a diverse array of environmental triggers that can interact with central dopamine functions.
The responses to unfamiliar animals vs. to familiar animals in our metals-treated voles are analogous to the responses to strangers vs. to mates by prairie voles after pair-bonding . Pair-bond formation in this species involves changes in D1-type dopamine receptor function in the ventral striatum. As a result of these changes, novelty-induced dopamine release that occurs during a subsequent encounter with an unfamiliar vole elicits an aversive rather than an affiliative response as would occur in a non-pair-bonded vole . We propose that similar processes might account for the social aversion often seen in autistic patients and hypothesize that changes in dopamine functioning which alter D1 receptor activation contribute to the deficits in social behavior seen in autistic patients. More specifically, in the case of metals exposure, decreased density or activity of dopamine clearance mechanisms may lead to abnormal synaptic levels of dopamine, resulting in increased D1 activation. Note that the changes associated with pair bonding are, to some extent, permanent. Pair-bonded voles that lose their mates rarely form a second pair bond, despite the fact that there may still be some social interaction with conspecifics . This may be analogous to the difficulties exhibited by some autistic patients in forming social attachments . Since the mechanism for adult pair-bonding likely shares common features with infant-parent bonds , the occasional lack of strong bonds between autistic patients and their parents  may be explained by alterations of dopamine systems that preclude the formation of appropriate social bonds. Thus, the competing hypotheses regarding processes underlying autism outlined by Buitelaar (impaired attachment v. approach-avoidance conflicts ) instead may reflect two aspects of the same fundamental processes in the brain (also see ).
One of the more striking results in the present study was the robust and consistent difference in the effects of metals ingestion between male and female voles. The sex differences in the aversive responses to a novel individual after metals treatment resemble the known differences in the incidences of autism in boys and girls. These behavioral differences were not due to sex differences in metal ingestion or to differences in accumulation of mercury in brain tissue, nor did they appear to be the result of generalized effects of long-term exposure to mercury since there were no differences in total water consumption or in animal weights at the end of the experiment. Sex-specific changes in locomotor activity associated with metals exposure such as those we found in the present study have previously been observed  and Aragona et al.  reported sex differences in both the rewarding aspects of amphetamine administration, and dopamine mediated changes in social affiliation. In each of these studies, the sex differences were attributed to sex differences in dopamine functioning, in the latter two cases, via differences in sensitivity to dopamine. It also should be noted that central dopamine interacts with the neuropeptides oxytocin (through co-localization of receptors in the nucleus accumbens), and vasopressin (via projections from the nucleus accumbens to the ventral pallidum) to modulate vole social interactions . An intriguing possibility is that sex differences in these neuromodulatory systems serve either to “protect” females from the effects of metals (oxytocin) or to make males more susceptible to metals effects (vasopressin).
But can altered dopamine function account for any of the other symptoms/behaviors associated with autism? We believe that the answer is at least a qualified “Yes”. Dopaminergic projections to the frontal cortex likely play a role in executive function and abnormal patterns of dopamine receptor activation can interfere with such function . Thus, altered dopamine functioning could contribute to the repetitive behaviors typically associated with autism. In fact, mice lacking the dopamine transporter display enhanced perseverative responses and have difficulty suppressing inappropriate responses , and take much longer to adapt to a novel situation . The possibility for dopamine involvement in autism is further supported by the observation that autistic patients displayed reduced activity in the frontal, striatal, and parietal regions when confronted with a task that required a shift in cognitive set . Finally, it should again be noted that the metals-induced social avoidance was limited to that toward strangers, while interactions with familiar animals were unimpaired. This may be an important parallel to the observation that autistic children performed on a par with control children in tasks with a predictable pattern, but showed deficits when no pattern was present . This latter observation may reflect the differences in mesolimbic dopamine system functioning when reward is predictable as opposed to unpredictable . In fact, changes in dopamine release associated with differences in predictability could account for the importance of a structured environment in treating autistic children .
This interpretation suggests that metals exposure should produce changes in other dopamine-mediated responses. To test this possibility, we examined another, non-social, dopamine-mediated behavior - locomotor responses to peripheral administration of amphetamine. Changes in locomotor responses to amphetamine have been reported after chronic exposure to manganese  and cadmium in rats, although the directions of the changes are not always consistent . We found that locomotor responses to amphetamine administration were reduced after mercury ingestion, which is consistent with other reports of effects of metals on dopamine mediated behaviors [56, 86] or dopamine metabolism , particularly within the mesolimbic dopamine system . The fact that the sex differences we observed in amphetamine responses after metals exposure paralleled the altered social behaviors strengthens the argument that the changes in social behavior may be dopamine-mediated. Together, these results further support our working hypothesis that changes in central dopamine function after metals exposure might contribute to the etiology of autism, and suggest involvement of the ventral striatum.
Many of the altered behaviors in the present study result from exposure to mercury. We chose to use mercury largely because more is known regarding the toxic effects of mercury than about any other metal save possibly lead. However, we recognize that any discussion of mercury in an autism context requires acknowledgement of the vaccine controversy [63, 64]. Although widely discredited [12, 59, 80, 85], the idea that thimerasol, a compound containing mercury and formerly used as a preservative in childhood vaccines, is responsible for autism, gained a life of its own . Parents, desperate for a solution, gravitated toward this idea, and many are reluctant to accept the scientific community’s assurances that preservatives in vaccines likely play little role in the development of autism. Unfortunately, for many in the autism field, this controversy has resulted in the mercury being thrown out with the thimerasol. In fact, mercury remains at least a potential contributing factor for autism , and should continue to be studied in that context. That having been said, however, it is important to note that a variety of metals such as lead, aluminum, cadmium, some of the lanthanides, copper and manganese (all multivalent cations) can negatively impact central dopamine metabolism[11, 53, 84, 95]. The fact that we were able to replicate the effects of mercury exposure by treating the animals with cadmium provides important support for the idea that it might be important to examine classes of environmental influences on the development of autism rather than looking for a specific cause. This suggestion is supported further by observations that environmental exposure to multiple toxins simultaneously is not only possible, but may in fact be likely . The existence of multiple insults, all of which can produce the same or similar symptoms, may account for the observed inconsistencies between individual exposure to specific stimuli and the incidence of the disorder. In this context, it should be noted that among a group of autistic patients , stimulated increases in excretion levels for three different metals were seen in different individuals (cadmium, lead, and mercury, respectively). Similarly, hair analysis revealed elevated mercury, lead, and uranium, although the data presentation does not allow comparison between individual subjects . Finally, it is important to note that, although we have concentrated on metals effects on dopamine-mediated behaviors, other environmental factors such as endocrine disruptors and pesticides also can alter dopamine function (reviewed by ). Together, these results suggest that future correlative and retrospective studies might find it valuable to test for exposure to a range of toxins instead of focusing on a single cause of autism.
One obvious caveat inherent in the present study is the fact that metals exposure occurred during adulthood while autism is a childhood-onset disorder. It is unknown at this point whether metals exposure during the perinatal period would produce similar changes in behavior. However, prenatal exposure to mercury produces sex specific changes in locomotor activity , and perinatal exposure to toxins has been shown to alter DAT function, perhaps permanently . More to the point, altered dopamine function during the perinatal period can be tied, at least indirectly, to autism. For example, Caesarian birth is a risk factor for autism  and can cause changes in dopamine receptor binding in rats . Importantly, in rats, these changes are not apparent until three months after birth , which may be an important parallel to the onset of autism typically occurring at 2–3 years of age in children. Further, some obstetrical complications can permanently alter the very dopamine systems  that we propose are responsible for the behavioral effects of metals that we observed in our voles. The present study establishes social interactions by prairie voles as an ecologically valid measure by which the effects of autism risk factors may be tested. Future studies may extend the model by exposing vole pups to metals during defined portions of the perinatal period and then examining their responses during social interactions as adults.
Significant strides have been made in understanding autism and animal studies have contributed to these advances. However, ultimate understanding of this disorder will require more than modeling the symptoms. To be of maximum utility, an animal model of autism must address three fundamental questions. First - What causes autism? Addressing this question will allow hypotheses regarding specific causative agents to be tested and will provide insights into potential environmental insults that may trigger the disorder. The second question is - How does it cause autism? Being able to address this question will allow not only hypothesis testing of the mechanism(s) by which a causative agent produces autism symptoms, but also will provide a framework for assessing different classes of causative agents. Answering the third question - Why does it cause autism? - will allow important insights into why two children in what seem to be identical circumstances do not both suffer from autism. When the ability to answer these questions can be combined in the same animal model, the rate of progress in understanding autism should increase significantly. Our results show that prairie vole social responses are sensitive to environmental influences at ecologically relevant doses and change in ways that are consistent with what already is known about autism. Here, a putative risk factor for autism (metals exposure) produced a symptom of autism (aversion to social novelty), possibly via changes in a neurotransmitter system known to affect social behavior (dopamine) in brain regions known to influence social behavior (mesolimbic areas) in a sex-specific fashion (autism is more prevalent in males). As a result, we have potential insight into two of three questions outlined above (What? and How?). We currently are examining how polymorphisms in genes associated with dopamine function may convey resistance or susceptibility to autism risks. The results of those studies may allow us to add an answer to the Why? question as well. In fact, we feel that the prairie vole model may well allow these questions to be addressed simultaneously.
Funding for this study was provided by National Institutes of Health grants to JTC (HD48462) and DRW (DA13137).
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