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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Vision Res. Author manuscript; available in PMC 2010 May 26.
Published in final edited form as:
PMCID: PMC2877124
NIHMSID: NIHMS16269

Arrestin can act as a regulator of rhodopsin photochemistry

Abstract

We report that visual arrestin can regulate retinal release and late photoproduct formation in rhodopsin. Our experiments, which employ a fluorescently labeled arrestin and rhodopsin solubilized in detergent/phospholipid micelles, indicate that arrestin can trap a population of retinal in the binding pocket with an absorbance characteristic of Meta II with the retinal Schiff-base intact. Furthermore, arrestin can convert Metarhodopsin III (formed either by thermal decay or blue-light irradiation) to a Meta II-like absorbing species. Together, our results suggest arrestin may be able to play a more complex role in the rod cell besides simply quenching transducin activity. This possibility may help explain why arrestin deficiency leads to problems like stationary night blindness (Oguchi disease) and retinal degeneration.

Keywords: Arrestin, Rhodopsin, Retinal, Metarhodopsin III, Photo-regeneration

1. Introduction

For over twenty years arrestin has been known as the ultimate quencher of light-induced rhodopsin signaling (Kühn, Hall, & Wilden, 1984; Wilden, Hall, & Kühn, 1986). It does so by binding to the phosphorylated form of light-activated rhodopsin, thus blocking further interaction of rhodopsin with transducin (Burns & Baylor, 2001; Hurley, Spencer, & Niemi, 1998; Ridge, Abdulaev, Sousa, & Palczewski, 2003). In this paper, we present evidence obtained in vitro that suggests interaction of arrestin with rhodopsin can also regulate the photochemistry of rhodopsin, which could have important functions in vivo.

The molecular details of the rhodopsin photo-cycle have been well studied. Brieffy, activation of rhodopsin (λmax ~ 500 nm) begins with light-induced isomerization of the covalently attached chromophore 11-cis retinal to all-trans retinal (Hubbell, Altenbach, Hubbell, & Khorana, 2003; Wald, 1968). This event leads to the formation of two photoproducts, Meta I (λmax ~ 480 nm) and Meta II (λmax ~ 380 nm), which are in equilibrium (Parkes, Gibson, & Liebman, 1999; Schertler, 2005). Meta II is the active signaling form of the receptor that couples to transducin (Burns & Baylor, 2001; Hamm, 2001).

Light-activated rhodopsin can decay by different but parallel pathways. The retinal Schiff-base of Meta II is sensitive to cleavage, and thus Meta II eventually decays to opsin and free retinal (Bownds & Wald, 1965; Farrens & Khorana, 1995). Alternatively, retinal Schiff-base isomerization in Meta I forms the long-lived photoproduct Meta III (λmax ~ 470 nm) (Vogel et al., 2003; Vogel, Siebert, Zhang, Fan, & Sheves, 2004b). Up to half of the rhodopsin in the human retina may be converted to Meta III in bright-light conditions (Lewis, van Kuijk, Carruthers, & Kliger, 1997). Meta III may thus exist for retinal storage purposes (Heck et al., 2003a), although it also retains some signaling ability (Zimmermann, Ritter, Bartl, Hofmann, & Heck, 2004).

Other wavelengths of light can cause further photo-conversions of rhodopsin (Hubbard & Kropf, 1958). For example, blue light (<420 nm) can convert Meta II into other photoproducts, including Meta III (Ritter, Zimmermann, Heck, Hofmann, & Bartl, 2004) and P500 (λmax ~ 500 nm) (Arnis & Hofmann, 1995). P500 appears to be similar to dark-state rhodopsin in its inactivity (Cone, 1967; Huddleston & Williams, 1977), although it might actually bind an all-trans retinal (Bartl, Ritter, & Hofmann, 2001).

In the current study, we address how arrestin can affect the formation and decay of these photoproducts. Understanding these processes is important for understanding the visual photo-cycle, especially since arrestin is likely to encounter these photoproducts in the light-exposed rod cell. We are exploring these questions using new in vitro approaches, which include a fluorescently labeled arrestin mutant, as well as a functional solubilized system in which to monitor arrestin–rhodopsin interactions (Sommer, Smith, & Farrens, 2005; Sommer, Smith, & Farrens, 2006). Here, we summarize this recent work, which shows that arrestin can stabilize Meta II, both by inhibiting retinal release and Meta III formation, and by interacting with Meta III and converting it to a Meta II-like species. We also present new data, which shows that irradiation of arrestin-bound Meta II can result in a P500-like product that no longer binds arrestin.

Finally, based on a review of the literature (see Section 4) as well as our results, we present a hypothesis that arrestin may have a biological role to limit retinal release in the photo-bleached retina, which could help protect the cell from long-term oxidative damage (Sparrow et al., 2003). In addition, we speculate that arrestin may regulate the photo-product Meta III, and this interaction may be necessary for proper dark adaptation (Lamb & Pugh, 2004).

2. Materials and methods

2.1. Materials

Except where noted below, all reagents were obtained from Sigma (St. Louis, MO). Frozen bovine retinas were obtained from Lawson and Lawson, Inc. (Lincoln, NE), and 11-cis retinal was a generous gift from Rosalie Crouch (Medical University of South Carolina and National Eye Institute). Monobromobimane was purchased from Molecular Probes (Eugene, OR), and spectroscopic-grade buffers were from USB Corporation (Cleveland, OH). Acrylamide/bisacrylamine solution (37:5:1) was purchased from Bio-Rad, and Concavalin A Sepharose was obtained from Amersham (Piscataway, NJ). N-dodecyl-β-d-maltopyranoside (DM) was from Anatrace (Maumee, OH), and asolectin was purchased from Fluka (Buchs, Switzerland). Asolectin, an inexpensive mixture of soybean phospholipids, is often used to reconstitute purified rhodopsin (Mansoor, Palczewski, & Farrens, 2006; Niu, Kim, & Khorana, 2002). It consists of roughly equal parts of phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol (product information as provided from manufacturer). To prepare asolectin/DM mixed micelles, a stock of 1% asolectin and 1% DM was made by dispersing the powdered asolectin into solution with a syringe and needle. The solution was clarified by centrifugation (100,000g, 20 min) before use.

2.2. Purification of native rhodopsin and recombinant arrestin

Rod outer segment (ROS) membranes containing native rhodopsin, and highly phosphorylated ROS, were prepared from bovine retinas. Phosphorylated rhodopsin (Rho-P) was purified from ROS using ConA and solubilized in DM as described previously (Sommer et al., 2005, 2006), and purified samples had a 280/500 nm absorbance ratio of 1.6. Mutant arrestin constructs W194F and I72C/W194F were created from the bovine visual arrestin cDNA (a gift from V.V. Gurevich) cloned in the pET15b vector (Invitrogen). All constructs were verified by DNA sequencing, arrestin was expressed and purified from Escherichia coli BL21(DE3) cells, and arrestin samples were subsequently labeled with monobromobimane as described (Sommer et al., 2006). Recombinant arrestin I72C/W194F labeled at ~92% efficiency; recombinant arrestin W194F labeled at less than 2% efficiency. No free label contamination was detected in the labeled arrestin samples. The bimane-labeled arrestin mutant I72C/W194F is subsequently referred to as I72B.

2.3. Fluorescence spectroscopy

All steady-state and time-resolved fluorescence measurements were made using a Photon Technologies steady-state spectrophotometer with a single excitation source (DeltaRam) and two emission detectors (T format). Excitation slits were set <0.25 nm to avoid bleaching of rhodopsin, and emission slits were set at 15 nm band pass. For emission scans, the sample was excited at 380 nm, and emission was measured from 400 to 600 nm using 2 nm increments (0.25 s integration per point). For simultaneous monitoring of retinal and arrestin release, the sample was excited for 1 s at 295 nm, followed by 1 s at 380 nm. Emission was detected at 330 and 456 nm, and the shutter was closed for 8 s between measurements. In this way, retinal release was monitored as an increase in opsin tryptophan fluorescence (λex: 295 nm, λem: 330 nm), and arrestin binding and release were monitored as changes in bimane fluorescence (λex: 380 nm, λem: 456 nm). Samples were photoactivated using a 150-W fiber optic light source (>495 nm). Background fluorescence was subtracted from all data, and steady-state scans were smoothed using the program Felix (Sommer et al., 2005, 2006).

2.4. UV–visible absorbance spectroscopy

All UV–visible absorption spectra were recorded with a Shimadzu UV-1601 spectrophotometer (bandwidth of 2 nm) (Sommer et al., 2006). For the photodecay experiments, the absorbance of 1 μM Rho-P (120 μl) was recorded in the dark after base-lining the spectrophotometer with the appropriate buffer. The sample was photoactivated (>495 nm) for 20 s, and spectra were subsequently recorded every 90 s for 120 min. For blue-light experiments, the sample was illuminated by a Machine Vision Strobe light source filtered through a 400 ± 20 nm band pass filter for 20 s. The presence of Schiff-base was ascertained by the addition of 5 μl of 0.8 N H2SO4 to the sample.

2.5. NaBH4 reduction and V8 proteolysis of rhodopsin

The fluorescent n-retinylidene opsin species (λex: 340 nm, λem: 480 nm) results from reduction of the Schiff-base in rhodopsin with NaBH4 (Bownds & Wald, 1965; Farrens & Khorana, 1995). Samples of 3 μM Rho-P, with or without 6 μM arrestin W194F, solubilized in 0.02% DM or 0.02% DM and 0.02% asolectin (20 μl) were photo-activated (>495 nm, 20 s, 20 °C), incubated in the dark for 120 min, and then 5 μl of 1% NaBH4 was added to each sample. After 10 min, 15 μl of 1 M sodium phosphate (pH 7.0) was added, and each sample was split into two 20 μl aliquots. Five microliters of 4.8 μM V8 protease was added to half the samples, and proteolysis occurred for 30 min at 20 °C. To assess the amount of Schiff-base present in Meta II rhodopsin immediately after activation, 1% NaBH4 was added to Rho-P (0.02% DM) in the dark, followed by photo-activation at 4 °C. Samples were subjected to 15% Tris–tricine–SDS–PAGE, and gels were soaked in 30% methanol before visualization. Bands were visualized using excitation from a short wave UV source (Alpha-Innotech FluorChem 5500 gel-doc) and a CCD camera (535 ± 50 nm cut-off filter; 10 min exposure). AlphaEase FC software was used to quantify the fluorescence of the bands.

2.6. Arrestin functional “pull-down” assay

Arrestin’s ability to bind Rho-P in native membranes (ROS-P) was performed as described previously with some modifications (Sommer et al., 2005). Briefly, a single sample of sonicated ROS-P (15 μM) was mixed with the fluorescently labeled arrestin mutant I72B (3 μM) in the dark (120 μl, 20 mM Hepes, 150 mM NaCl, pH 7.4, 20 °C). 20 μl aliquots were removed and immediately placed on ice in the dark, after green light illumination (>495 nm, 60 s), after subsequent blue-light illumination (<420 nm, 60 s), after subsequent green light illumination (60 s), and after the addition of 10 mM hydroxylamine. The sample was illuminated from the top to prevent the plastic tube from filtering the light. The samples were then diluted 10-fold with ice-cold buffer, centrifuged at 100,000g for 10 min (4 °C), and the pellets were solubilized in loading buffer and subjected to SDS–PAGE (10%). The gel was visualized as described above using a gel-doc apparatus, and bands were quantified using the AlphaEase FC software.

3. Results

3.1. Fluorescent arrestin binding to rhodopsin in mixed micelles

Arrestin binding and release from light-activated phosphorylated rhodopsin (Rho*-P) in native ROS membranes can be monitored with fluorescently labeled arrestin mutants (Sommer et al., 2005). Specifically, bimane-labeled arrestin I72C (I72B) exhibits a blue-shift and increase in fluorescence upon binding Rho*-P. The blue-shift indicates the probe is relocated into a more hydrophobic domain (Mansoor, McHaourab, & Farrens, 1999). Thus, this fluorescence change is most likely due to a burying of the bimane probe in a protein-protein or a protein-lipid interface. Fig. 1 illustrates the location of the fluorescent probe on arrestin and the likely binding interfaces between rhodopsin and arrestin.

Fig. 1
Cartoon representation of the experimental system used in this study. Possible arrangement of dark-state rhodopsin (gray) and a hypothetical dimer partner (light gray) (Liang et al., 2003) in a mixed detergent/phospholipid micelle. The intradiskal and ...

Using this approach, we recently made an unexpected discovery. We found that although arrestin binding to Rho*-P is inhibited when Rho-P is purified and solubilized in n-dodecyl-β-d-maltopyranoside (DM), arrestin binding can be restored with the addition of exogenous phospholipids in the form of asolectin (Fig. 2A) (Sommer et al., 2006). Furthermore, we found that acidic phospholipids (phosphatidylserine, phosphatidylinositol, and phosphatidic acid) specifically enable arrestin binding (Sommer et al., 2006). The high phosphatidylinositol content (~30%) of asolectin probably explains why it serves as a good enhancer of arrestin binding to DM-purified Rho-P.

Fig. 2
Arrestin can trap ~half of the retinal population in opsin. (A) Fluorescence of arrestin I72B (1 μM) in the presence of a twofold excess of purified Rho-P in DM-micelles (left panel) or DM/asolectin mixed micelles (right panel). Smoothed spectra ...

Importantly, the ability to monitor arrestin binding to Rho-P solubilized in mixed DM/asolectin micelles enables a soluble system in which to study the dynamics of the interaction of arrestin with the major photoproducts of rhodopsin and how this influences the release of the retinal chromophore from the binding pocket.1 Our results are described below.

3.2. Arrestin traps a population of retinal in mixed micelles

We measured retinal release from Rho*-P solubilized in mixed DM/asolectin micelles by monitoring the increase in native opsin tryptophan fluorescence that occurs when retinal is released from the binding pocket (Farrens & Khorana, 1995) (Fig. 2B). The data show that retinal was released from Rho*-P in mixed micelles with a t1/2 = 12.3 ± 0.8 min, which is ~1.6 times slower than in native ROS membranes (Sommer et al., 2006). The addition of hydroxylamine, a compound that cleaves the Schiff-base, resulted in a small (~5%) increase in fluorescence.

Strikingly, in the presence of a twofold excess of arrestin, the tryptophan fluorescence of Rho*-P plateaued at ~half that seen in the absence of arrestin, implying that arrestin traps ~half of the retinal population in the binding pocket (Heck et al., 2003a). The addition of hydroxylamine caused the fluorescence to increase to the same level as seen in the absence of arrestin. Interestingly, arrestin appeared to only affect the plateau of tryptophan fluorescence and not the rate at which the fluorescence increased (t1/2 = 13.2 ± 0.7 min).

The fluorescent arrestin I72B was used to monitor arrestin binding and release during the same experiment described above (Fig. 2C). Arrestin binding to Rho*-P in mixed micelles resulted in a ~50% increase in bimane fluorescence. The rate of arrestin release was significantly slower (t1/2 ~ 25 min) compared to retinal release, and the bimane fluorescence plateaued at ~70% of the starting state intensity.2 Hydroxylamine returned the fluorescence to the starting-state level.

Note that, at this time, we are not able to correlate the relative fluorescence change of I72B to the concentration of Rho*-P-bound arrestin, so we cannot quantify the ratio of bound arrestin to trapped retinal. Thus, we can only conclude that while some arrestin remained bound in the complex, ~ half of the retinal population was trapped.

3.3. Arrestin traps retinal as a Schiff-base adduct

We determined that the trapped retinal observed in Fig. 2B represents a Schiff-base linked form, by reducing the retinal Schiff-base with NaBH4, which results in a fluorescent n-retinylidene species (Bownds & Wald, 1965; Farrens & Khorana, 1995). Subsequent SDS–PAGE enabled the separation the n-retinylidene opsin from possible retinal–phospholipid adducts (Sommer et al., 2006). We also digested the reduced opsin with V8 protease to reveal the relative location of the covalently attached retinal on the protein. V8 cleaves opsin in the third cytoplasmic loop, resulting in two fragments. The larger fragment F1 (~27 kDa) contains transmembrane helices 1–5, and the smaller fragment F2 (~15 kDa) consists of transmembrane helices 6 and 7 (Farrens, Altenbach, Yang, Hubbell, & Khorana, 1996). Fragment F2 also contains Lys296, to which retinal is attached in dark-state and Meta II rhodopsin (Bownds, 1967).

When Rho-P in DM-micelles was reduced immediately after photo-activation (Meta II), retinal is clearly attached to F2 (Fig. 3B, lane 6). Two hours after photo-activation, ~25% of the original retinal population was attached to opsin (Fig. 3A, lane 7). The V8 proteolysis assay revealed some of this retinal is attached to both F1 and F2 of Rho-P (Fig. 3B, lane 7). This residual retinal may represent Meta III as well as retinal which attached to peripheral lysines on Rho*-P after release from the binding pocket (Fishkin, Jang, Itagaki, Sparrow, & Nakanishi, 2003). Note that the fluorescence of the V8 protease implies that some retinal may attach to this enzyme as well. In the presence of arrestin (Fig. 3B, lane 8), the amount of retinal attached to opsin in DM micelles was not significantly changed.

Fig. 3
Arrestin traps retinal as a Schiff-base adduct on F2 of Rho*-P. (A) Reducing the retinal Schiff-base with NaBH4 produces a fluorescent Rho*-P that indicates the amount of retinal covalently attached to opsin. Briefly, Rho-P solubilized in 0.02% DM or ...

In DM/asolectin mixed micelles, only ~10% of retinal remained attached to opsin after photodecay (Fig. 3A, lane 9). Why is this value lower than in DM alone? A likely explanation is that the phosphatidylethanolamine in asolectin competes with lysine residues for retinal (Plack & Pritchard, 1969; Sparrow et al., 2003), and this conclusion is supported by the presence of a low molecular-weight fluorescent species (Fig. 3B, lanes 9 and 10). Significantly, arrestin caused ~60% of the retinal to remain attached to opsin, and most of this retinal (~80%) was attached to F2 (Fig. 3B, lane 10). Besides illustrating the phospholipid-dependence of retinal trapping by arrestin, these results confirm that arrestin acts to trap ~ half the retinal in opsin, as suggested from the plateau in rhodopsin tryptophan fluorescence (Fig. 2B). In addition, it appears that arrestin traps retinal on F2 of rhodopsin, possibly at Lys296.

3.4. Arrestin blocks Meta III formation and traps spectral Meta II

The photo-intermediates of Rho-P in DM/asolectin micelles were assayed using UV–visible absorption spectroscopy (Fig. 4A). Dark-state Rho-P exhibited a characteristic absorption maximum at 500 nm that shifted to 380 nm after photo-activation. Over time, there was a loss of absorbance at 380 nm and an increase in absorbance between 450 and 480 nm. This event can be explained as the conversion of Meta II (380 nm) to Meta III (470 nm) and the release of retinal from the binding pocket to form adducts with phospholipid head-groups (440–450 nm).

Fig. 4
Arrestin stabilizes Meta II and converts Meta III to spectral Meta II. (A) The absorbance of Rho-P in DM/asolectin mixed micelles was observed in the dark (thick solid spectrum) and after photoactivation (dashed spectrum). Spectra were subsequently recorded ...

However, when an excess of arrestin was present, the photodecay absorption spectra of Rho-P were dramatically different (Fig. 4B). There was a significant inhibition of the absorbance loss at 380 nm, and the increase of absorbance between 450 and 480 nm was inhibited. These data suggest that arrestin stabilizes rhodopsin in a Meta II-like state and inhibits retinal release and Meta III formation.

The presence of Schiff-base was assessed by acidification of the samples after photodecay in the experiments described above (Fig. 4C). Protonated Schiff-bases absorb at 440 nm and are indicative of retinal attached to opsin (as in the case of Meta II and Meta III) or linked to phospholipid. In the absence of arrestin, addition of acid to photo-decayed Rho*-P resulted in a broadened absorbance (λmax ~ 416 nm) that is typical for retinal adducts with asolectin phospholipids. In the presence of arrestin, acidification resulted in a 440 nm absorbance peak that verifies the NaBH4-reduction data (Fig. 3): arrestin traps retinal in a Schiff-base linked form.

3.5. Arrestin converts thermal Meta III to spectral Meta II

The above results clearly show that arrestin can prevent accumulation of Meta III. We next tested whether Meta III could interact with arrestin directly, as it can with transducin (Zimmermann et al., 2004). 3 The ability to measure this interaction in detergent allows clear observation of rhodopsin’s spectral intermediates free of scattering artifacts. With membranes, these experiments are complicated by the fact that arrestin binding to vesicles results in a significant increase in scattering (Schleicher, Kühn, & Hofmann, 1989).

Adding arrestin to Rho*-P 27 min after photoactivation (20 °C) resulted in a clear decrease at 470 nm (Meta III) and increase at 380 nm (Fig. 4D). This 380 nm species is characterized by an intact Schiff-base, and is thus likely Meta II (Fig. 4D, inset). Although some decay of Meta III in the absence of arrestin was observed, arrestin clearly speeds its depletion (Fig. 4E). Furthermore, in the absence of arrestin, no significant increase in absorbance at 380 nm was observed as Meta III decays, because Meta II was not stabilized (data not shown). Note that in the presence of arrestin, the absorbance at 475 nm did not return to the starting state level, probably because retinal-phospholipid adducts absorbing in that region were formed before the addition of arrestin.

3.6. Arrestin interacts with the blue-light photoproduct P470 but not with photoproduct P500

We next explored arrestin’s interaction with the photoproducts that result from blue-light irradiation of Meta II, which include species that absorb at ~470 and ~500 nm (called P470 and P500, respectively) (Arnis & Hofmann, 1995; Ritter et al., 2004). Although P470 has been shown to be identical to Meta III (Ritter et al., 2004), the identity of P500 remains unresolved. Long-believed to be photo-regenerated rhodopsin (Arnis & Hofmann, 1995), P500 may in fact bind all-trans retinal (Bartl et al., 2001). In addition, the relative proportions of P470 and P500 that form after irradiation of Meta II depend on the pH used and may differ between detergent- and membrane-bound rhodopsin (Arnis & Hofmann, 1995; Bartl et al., 2001; Ritter et al., 2004). It is important to note that in the preliminary experiments described below, we observed species that absorb at ~470 and ~500 nm, but we have not formally shown that these are the same P470 and P500 described above. In addition, we cannot rule out the possibility that P500 in our experiments is photo-regenerated dark-state rhodopsin.

The properties of blue-light photoproducts in mixed micelles are illustrated in Fig. 5A and B. When Meta II (Fig. 5A, curve ii) is illuminated with blue light (<420 nm), two absorbance peaks were formed (Fig. 5A, curve iii): unconverted Meta II (λmax ~ 380 nm) and the “P-products” (λmax ~ 480 nm). When hydroxylamine was added to these photoproducts (Fig. 5B, curve iv), absorbance peaks corresponding to retinaloxime (λmax ~ 365 nm) and P500 (λmax ~ 500 nm) were observed. Thus, while P470 is sensitive to hydroxylamine, P500 is thermally stable and resistant to hydroxylamine. When P500 was illuminated with green light in the presence of hydroxylamine, retinaloxime resulted (Fig. 5B, curve v), suggesting that green light converts P500 to a form that is hydroxylamine sensitive, like Meta II.

Fig. 5
Arrestin interacts with the blue-light photoproduct P470 but not P500. (A) The absorbance spectra of Rho-P were recorded (i) in the dark state, (ii) after illumination with green light (>495 nm, 20 s), and (iii) after subsequent illumination with ...

The population of “P-products” formed from the blue-light irradiation of Meta II showed a gradual loss of 470 nm absorbance and gain of 380 nm absorbance over time (Fig. 5C). We assume this is due to thermal decay of P470 to Meta II and/or opsin and free retinal. Since P470 is equivalent to Meta III (Ritter et al., 2004), we were curious if arrestin would also interact with P470. Thus, we added arrestin to a sample of blue-light irradiated Meta II and found that arrestin speeds the conversion of the ~470 nm species to a Meta II-like species (λmax ~ 380 nm) (Fig. 5D). Furthermore, the relative intensity of the 380 nm peak two hours after illumination was much greater in the presence of arrestin. Since Meta II is more absorptive than free retinal, this result suggests that arrestin can interact with P470 and convert it to Meta II, which it stabilizes. In the absence of arrestin, P470 appears to decay ultimately to opsin and free retinal.

Interestingly, the rates at which arrestin converts thermal Meta III (Fig. 4E, t1/2 ~ 10 min) and P470 (Fig. 5D, t1/2< 1.5 min) are significantly different, even though similar amounts of arrestin were used in these experiments (1.5 and 2 μM, respectively). We are unsure of the cause of this apparent discrepancy, but it is worth noting that the rhodopsin photoproduct population differed in these experiments. When arrestin was added to photo-decayed Rho*-P (Fig. 4E), a significant amount of Meta II was present (~25% of original population), which would have competed for arrestin binding to Meta III. This complicated situation may explain why the loss of Meta III seen in Fig. 4E is not described by a simple single-exponential rate (data not shown). In contrast, the blue light irradiation used in Fig. 5D resulted in a greatly depleted population of Meta II, which would have made much more arrestin available for interaction with P470.

Our data also suggest that arrestin does not interact with P500, since we find that a hydroxylamine-insensitive peak (λmax ~ 500 nm) remained, even after arrestin was added to blue-light irradiated Meta II (data not shown).4 We explored this possibility further by monitoring arrestin I72B binding after blue-light illumination of Meta II (Fig. 5E). Note that blue-light irradiation of arrestin-bound Meta II caused a dramatic drop in the fluorescence of arrestin, suggesting that blue light releases a significant fraction of arrestin. We could restore arrestin binding with subsequent green-light illumination (note the increase in fluorescence), and hydroxylamine-catalyzed decay of Meta II returned the fluorescence to the dark-state intensity.

Biochemical “pull-down” analysis using membrane-bound Rho*-P confirmed these results (Fig. 5F). Interestingly, the fluorescence profile in Fig. 5F closely mirrors that in Fig. 5E. Together, these results suggest arrestin cannot bind P500 since blue-light irradiation of arrestin-bound Meta II causes dissociation of arrestin. However, because arrestin can bind P470, some arrestin remains bound after blue-light irradiation. Finally, green light can convert P500 to a form which arrestin can bind.

4. Discussion

4.1. Overview of paper

We investigated arrestin binding to Rho*-P in mixed detergent (DM)/phospholipid micelles to assess how arrestin can regulate rhodopsin photochemistry, in a system unencumbered by the optical artifacts present in membrane-bound rhodopsin.

Our findings are summarized in Fig. 6. Briefly, we find that arrestin can stabilize ~half the population of retinal in a Schiff-base linked form with a Meta II-like absorbance (Figs. 24). In this stabilized form, retinal is likely attached to Lys296, although we cannot exclude the possibility that retinal may have migrated to another nearby site, perhaps near Helix VIII (Schädel et al., 2003).

Fig. 6
A cartoon proposing how arrestin can interact with the major photoproducts of phosphorylated rhodopsin. Dark state rhodopsin (Rho) is converted by green light (>495 nm) to Meta I (MI), which is in equilibrium with Meta II (MII). The Meta I/Meta ...

Furthermore, we find that arrestin clearly inhibits Meta III formation (Fig. 4B). Arrestin is known to favor Meta II at the expense of Meta I (Schleicher et al., 1989), and since Meta III evolves from Meta I (Vogel et al., 2004b), these facts may explain why we see Meta III inhibition in the presence of arrestin. However, we also find that arrestin can interact with thermal and blue-light-generated Meta III and convert it to a species with Meta II-like characteristics (Figs. 4 and and5D).5D). The fast conversion of Meta III to Meta II may also explain the ability of arrestin to inhibit Meta III formation.

Finally, we find that arrestin does not interact with the blue-light photoproduct P500, although green light can convert P500 to a form to which arrestin can bind (Fig. 5). To the best of our knowledge, these results provide the first insights into the effect of blue-light on arrestin–rhodopsin interactions and suggest further investigations are warranted.

4.2. Caveats for this study

There are several caveats that must accompany the results reported here. Although the functional soluble system described here enables new insight into how arrestin regulates rhodopsin photochemistry, mixed detergent/lipid micelles obviously differ from the native ROS membrane in several ways. For example, solubilization in detergent is known to favor Meta II, perhaps by lowering the activation energy for the Meta I → Meta II transition (König, Welte, & Hofmann, 1989; Litman, Kalisky, & Ottolenghi, 1981). This may be why we do not observe the significant levels of Meta III observed in membranes (up to 40%) (Heck et al., 2003a). Furthermore, we have not formally shown that the Meta III and blue-light photoproducts we observe are the same as those reported in membranes.5

The presence of detergent may also disrupt possible secondary retinal-binding pockets and could thus affect the hypothesized channeling of retinal through the protein during release (Heck, Schädel, Maretzki, & Hofmann, 2003b; Schädel et al., 2003). The possible linkage between our results and the prior reports on retinal channeling is especially intriguing, considering the differences in retinal and arrestin release in micelles versus membranes. In native ROS membranes, arrestin slows retinal release, and arrestin and retinal release occur at the same time (Hofmann et al., 1992; Sommer et al., 2005). In contrast, only ~half of retinal is released and arrestin releases only very slowly in detergent/lipid micelles (Fig. 2 and (Sommer et al., 2006)). This phenomenon might suggest a coupling of retinal channeling and arrestin release. Interestingly, addition of detergent to rhodopsin-containing membrane extracts from Drosophila has also been reported to decouple arrestin release and the rates of regeneration in the invertebrate visual cycle (Kiselev & Subramaniam, 1996).

Another possible explanation for the “decoupling” of retinal and arrestin release in detergent is related to the phosphorylation status of our rhodopsin samples. Although our phosphorylation procedure yielded an average of ~6 phosphates per rhodopsin (Sommer et al., 2005), and thus makes a large population of nonphosphorylated rhodopsin unlikely, we cannot rule out the possibility that a population of rhodopsin exists that contains only one or two phosphates. Perhaps arrestin requires higher levels of phosphorylation (>2) in order to form a long-lived complex with rhodopsin in mixed micelles. We are currently investigating this possibility, by separating the different species of phosphorylated rhodopsin as described (Adamus, Arendt, Hargrave, Heyduk, & Palczewski, 1993; Aton, Litman, & Jackson, 1984).

Finally, the possible dimerization of rhodopsin might affect the dynamics of arrestin and retinal release (Liang et al., 2003; Mansoor et al., 2006). For example, our data is consistent with a scenario where arrestin(s) binds a dimer of rhodopsin, and retinal is released from only one dimer partner (Sommer et al., 2006). However, we cannot rule out the possibility that, in mixed micelles, rhodopsin may dimerize in a head-to-tail fashion, as observed in the crystal structure (Palczewski et al., 2000). We cannot predict what effect this manner of dimerization might have on arrestin binding.

Despite these unknowns, the preliminary results we present here represent valuable insight into how arrestin regulates retinal release and photoproduct formation from rhodopsin. In the following discussion, we speculate about the possible implications of our results in the visual photo-cycle.

4.3. Possible mechanisms of arrestin translocation

Recent studies have explored the mechanisms underlying the massive light-dependent translocation of arrestin, which appears important for light adaptation in the rod cell (Elias, Sezate, Cao, & McGinnis, 2004; Nair et al., 2004; Peterson et al., 2003; Strissel, Sokolov, Trieu, & Arshavsky, 2006). In the dark-adapted rod cell, arrestin is normally sequestered in the inner segment, where microtubules might serve as a “sink” for arrestin binding (Hanson, Francis, Vishnivetskiy, Klug, & Gurevich, 2006; Nair et al., 2004). Upon exposure to light, arrestin translocates from the inner segment to the outer segment, where it remains until the removal of light (Elias et al., 2004). This translocation has been proposed to be governed by mass action, where arrestin’s affinity for Rho*-P keeps it in the outer segment (Nair et al., 2005). However, other studies suggest that more complex mechanisms, including active transport, might be involved (Mendez, Lem, Simon, & Chen, 2003; Peterson et al., 2005; Strissel et al., 2006; Zhang et al., 2003). Remarkably, there may be enough arrestin in the rod to bind the entire pool of rhodopsin in a completely photo-bleached cell (Strissel et al., 2006). Since little regeneration of rhodopsin probably takes place under constant illumination (Palczewski et al., 1999), the persistence of arrestin in the outer segment suggests that it might be interacting with a long-lived photoproduct of rhodopsin. This possibility is supported by the results we present in this paper.

The fact that arrestin only enters the rod outer segment in bright light, when the rod cell becomes nonfunctional, suggests arrestin may do more than simply act as a classical signal terminator. It is more likely that p44, the short splice variant of arrestin, regulates rhodopsin signaling in the dim-light operational range of the rod cell (Langlois, Chen, Palczewski, Hurley, & Vuong, 1996). Consistent with this role, p44 is present in the outer segment even in the dark (Smith et al., 1994). The difference in dark-state localization between arrestin and p44 might be due to the high affinity of p44 for dark phosphorylated rhodopsin and opsin, which may constitute ~1% of the rhodopsin in the dark-adapted rod cell (Binder, O’Connor, Bownds, & Arshavsky, 1996; Pulvermüller et al., 1997; Schröder, Pulvermüller, & Hofmann, 2002). Although p44 has a similar affinity for dark Rho-P and Rho*-P, experiments using a fluorescently labeled p44 analogous to arrestin I72B suggest that p44 binds dark Rho-P differently than Rho*-P (Sommer, Smith, & Farrens, unpublished results). The localization of p44 to the ROS membrane may be a key determinate in its ability to quench Rho* activity quickly (illustrated in Fig. 7) (Langlois et al., 1996; Schröder et al., 2002). Since p44 is expressed at only ~10% the levels of arrestin, there is sufficient p44 to bind the limited number of Rho* produced by dim light (Palczewski et al., 1994). As described below, we postulate that an unappreciated role for full-length arrestin in the rod cell is a regulator of late photo-products and retinal release, rather than simply a quencher of Meta II signaling.

Fig. 7
Model of light-dependent attenuation of Rho* by p44 and arrestin. In the dark-adapted rod disk (upper panel), p44 (light blue) is “tethered” to the membrane by its interaction with Rho-P (red), which comprises ~1% of the dark-state rhodopsin ...

4.4. Possible physiological implications of arrestin’s interaction with metarhodopsin III

A form of stationary night blindness called Oguchi disease is a recessive genetic disorder caused by mutations in either rhodopsin kinase or arrestin. Patients with Oguchi disease require 5–7 h to dark-adapt, which is much longer than the normal 40 min (Dryja, 2000; Lamb & Pugh, 2004). This phenomenon suggests that arrestin activity is necessary for proper dark adaptation, which involves the removal of all metarhodopsin species from the rod cells and the regeneration of rhodopsin with 11-cis retinal. In bright light conditions, up to half of the rhodopsin in the human retina is estimated to be converted to Meta III (Lewis et al., 1997). Lamb and Pugh have hypothesized that Oguchi disease is caused by a build-up of Meta III, which decays much slower in the absence of arrestin and thus prevents regeneration (Lamb & Pugh, 2004).

Our results support this hypothesis. We find that phosphorylated Meta III can be bound by arrestin and stabilized as a Meta II-like species. In contrast to Meta III, arrestin-bound Meta II may be more accessible to the regeneration machinery, which begins with the reduction of all-trans retinal by retinol dehydrogenase (Hofmann et al., 1992). Furthermore, arrestin may serve to attenuate Meta III signaling by competing with transducin (Zimmermann et al., 2004).

4.5. Arrestin may help limit retinal release to prevent retinal degeneration

Arrestin knock-out mice, as well as some Oguchi disease patients, suffer light-dependent retinal degeneration, which may be due to excessive signaling (Chen, Simon, Matthes, Yasumura, & LaVail, 1999; Dryja, 2000; Hao et al., 2002). However, this phenomenon may not always be caused by excessive signaling, since bright light-induced retinal degeneration in arrestin knock-out mice is independent of transducin (Hao et al., 2002). Furthermore, bright light-induced retinal degeneration is characterized by an induction of the transcription factor AP-1, which suggests that oxidative stress might be the cause (Reme, 2005).

How might bright light trigger this pro-apototic redox-sensitive transcription factor? In contrast to other visual processes, the reduction of retinal to retinol occurs quite slowly (tens of minutes), which might be due to the low enzymatic activity of retinol dehydrogenase (Saari, Garwin, Van Hooser, & Palczewski, 1998), although other mechanisms might also be involved (Chen et al., 2005). Thus, in bright-light conditions, levels of free all-trans retinal might become quite high, which can lead to the formation of di-retinal conjugates with phosphatidylethanolamine (A2E). Light-dependent oxidation of A2E yields dangerous epoxides, which may be a causative agent in both age-related (AMD) and Stargardt’s macular degeneration (Radu, Mata, Bagla, & Travis, 2004; Sparrow et al., 2003; Wenzel, Grimm, Samardzija, & Reme, 2005). Note that animals lacking arrestin are susceptible to retinal damage from bright light, supporting the suggestion that arrestin serves a protective role in the rod cell. Consistent with this hypothesis, arrestin expression appears to be up-regulated when animals are exposed to light (Organisciak et al., 1991), as is our finding that arrestin can limit the release of retinal and trap it in stabilized Meta II.

A possible contradiction to this hypothesis is presented in an in vivo study utilizing arrestin knock-out mice (Palczewski et al., 1999). The authors found no significant difference in the retinoid levels in wild-type and arrestin knock-out mice, suggesting that arrestin does not play a critical role in regulating all-trans retinal release. However, these experiments used dark-adapted mice exposed to a bright flash of light. Under these conditions, arrestin would be mostly sequestered in the inner segment of the rod cell at the time of the flash, and this might explain why they observed no major difference between wild-type and arrestin knock-out mice. Arrestin’s effect might be more pronounced in mice which are exposed to constant illumination. Also, note that even a slight increase in the amount of free retinal caused by a lack of arrestin could, over time, lead to an increase in the levels of A2E and other damaging retinal byproducts.

It is interesting to note that when wild-type mice were exposed to constant illumination, levels of all-trans retinal remained elevated (at ~20% of total retinoids) while the levels of down-stream metabolic products (retinol) plateaued (Palczewski et al., 1999). This result might imply that arrestin stabilized a population of Meta II in the mouse retina. This possibility would be similar to the invertebrate system, where arrestin stabilizes Metarhodopsin and inhibits the release of retinal (Kiselev & Subramaniam, 1996, 1997). It is also interesting to consider that certain rhodopsin mutations, which constitutively activate the receptor and are implicated in Retinitis Pigmentosa, might lead to inappropriately long-lived arrestin–rhodopsin complexes and cytotoxic aggregates (Chuang, Vega, Jun, & Sung, 2004; Li, Franson, Gordon, Berson, & Dryja, 1995; Rim & Oprian, 1995). Finally, it has recently been suggested that retinol dehydrogenase activity may not be the sole factor that contributes to the relatively slow conversion of all-trans retinal to retinol in the rod cell (Chen et al., 2005). It is possible that the stabilization of Meta II by arrestin also contributes (Hofmann et al., 1992).

4.6. Possible roles of the blue-light photoproducts

In vitro studies suggest that rod cell exposure to full-spectrum light might generate P500 as well as Meta III (Arnis & Hofmann, 1995; Bartl et al., 2001). Although Meta III appears to have some activity, since it is bound by both transducin and arrestin, P500 seems to be similar to dark-state rhodopsin in its inactivity. In vivo investigations have shown that blue-light irradiation can deactivate a bleached rod cell (Cone, 1967; Grimm, Reme, Rol, & Williams, 2000; Huddleston & Williams, 1977), presumably by regenerating rhodopsin or isorhodopsin, which binds 9-cis retinal. Thus, the blue-light photoproduct absorbing at ~500 nm might represent another storage form of the protein, and as such it would make sense that it would not interact with arrestin. Since P500 can undergo light-dependent activation to a Meta II-like species, this photoproduct might serve to preserve some light-sensitive pigment in the rod cells after being exposed to high-bleaching conditions. This mechanism would allow the cell to maintain some sensitivity when transitioning from bright to dim light conditions before dark adaptation has occurred.

5. Summary

We described the following results for arrestin–rhodopsin interactions in a detergent-solubilized system: (1) Arrestin can inhibit some retinal release from rhodopsin. The retinal is trapped in a Schiff-base linked form, most likely at Lys296, with an absorbance characteristic of Meta II (380 nm). (2) Arrestin can interact with Meta III, formed either by thermal decay or blue-light irradiation, and convert it to a Meta II-like species. (3) Arrestin does not interact with the blue-light photoproduct P500, but green light can convert P500 to a form which arrestin can bind. Although these results may be unique for arrestin–rhodopsin interactions in a detergent-solubilized system, they do suggest the possibility that another role for arrestin in the rod cell might be to limit retinal release and regulate Meta III, which is consistent with recent physiological evidence supporting this hypothesis.

Acknowledgments

This work was supported in part by National Institutes of Health Grants DA018169 and EY015436 (to D.L.F.), a National Defense Science and Engineering Graduate Fellowship (to M.E.S.), and an N.L. Tartar Research Fellowship (to M.E.S.).

Footnotes

1An earlier study also looked at arrestin–rhodopsin interactions using detergent-solubilized rhodopsin (Imamoto, Tamura, Kamikubo, & Kataoka, 2003). However, as far as we are aware, our study is the first to examine the effect of arrestin on retinal release and photoproduct formation using detergent-solubilized rhodopsin.

2The plateau reported here is higher than in our previously published data (Sommer et al., 2006), even though both experiments used DM/ asolectin mixed micelles. We do not know the cause of this variation, but it may be due to the difference in background fluorescence due to the heterogenous nature of this phospholipid source. However, the retinal release as ascertained by opsin tryptophan fluorescence consistently plateaus at 50%.

3Previously, it was shown that arrestin can convert 470 nm-absorbing “pseudo-photoproducts” of rhodopsin, which consist of nonspecific complexes of membrane-bound phosphorylated opsin and all-trans retinal, into species absorbing at 380 nm (Hofmann, Pulvermüller, Buczylko, Van Hooser, & Palczewski, 1992).

4Note that blue-light illuminated arrestin-bound Meta II produced a similar distribution of absorbing species as in the absence of arrestin, suggesting that arrestin cannot inhibit the conversion of Meta II to the P-products (data not shown). In this regard, arrestin may be different than transducin, which can inhibit the formation of P500 (Arnis & Hofmann, 1995; Bartl et al., 2001).

5This would require FTIR analysis of the protein as well as isolation and identification of the conformation of the retinal chromophore, as described (Bartl et al., 2001; Heck et al., 2003a; Vogel et al., 2003; Zimmermann et al., 2004).

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