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|Cover: To examine off-target gene silencing in RNA interference, the computer-generated conformations of siRNA/target duplexes that include a single target mutation are used to mimic the silencing of imperfectly-matched genes. The illustration shows that both the position and the identity of the introduced mutation contribute to conformational distortion of the effector duplex, and the distortions are further correlated with gene silencing of such targets. These findings provide evidence that conformational distortion of siRNA/target duplexes due to target mismatch plays an essential role in off-target gene silencing.|
|For further information, please see the paper by Huang et al., Nucleic Acids Res., 2009, 37, 7560–7569.|