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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
J Immunol. Author manuscript; available in PMC 2010 June 1.
Published in final edited form as:
J Immunol. 2010 June 1; 184(11): 6060–6066.
Published online 2010 April 28. doi: 10.4049/jimmunol.1000511

Figure 6

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KLF2 and S1P1 are required for homing of γδ and CD8αα+ αβ IEL to the gut

Mixed bone-marrow chimeras were created with equal ratios of wild-type competitor and VavCre (control), Klf2fl/flVavCre, or S1pr1fl/flVavCre marrow. Cells were isolated from thymus and small intestine and stained for CD4, CD8α, CD8β, CD45.1, CD45.2, TCRδ and TCRβ and analyzed by flow cytometry. To allow comparison between different experiments, we normalized the ratios relative to the ratio in DP thymocytes. The effect of KLF2 and S1P1 deficiency in γδ T cells (top panel) and CD8αα+ αβ T cells (bottom panel) are indicated. Error bars indicate the standard deviation. n = 3 chimeras per genotype and three independent experiments. **p < 0.001 (for γδ T cells),*p < 0.02 (for CD8αα+ αβ T cells)

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