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Curr Opin Genet Dev. Author manuscript; available in PMC 2010 May 19.
Published in final edited form as:
Curr Opin Genet Dev. 1994 August; 4(4): 529–534.
PMCID: PMC2873142
NIHMSID: NIHMS197072

The making of a maggot: patterning the Drosophila embryonic epidermis

Abstract

Cell fates are instructed by signals emitted from specialized cell populations called organizers. The study of epidermal patterning in Drosophila is contributing novel insights concerning the establishment and action of such organizers. Juxtaposed rows of cells express either the wingless or hedgehog signaling molecules and thereby act as organizers of segment pattern. These signals mediate a mutually re-enforcing interaction between the two rows of cells to sustain organizer function. In a distinct and subsequent phase, wingless and hedgehog act to specify the fates of cells.

Introduction

One of the early successes of experimental embryology was the demonstration of the importance of induction to embryonic development. In this process, organizing centers emit signals that direct the choice of fate in surrounding cells [1]; for example, a signaling center in the posterior portion of the limb bud, the zone of polarizing activity (ZPA), organizes the pattern of digits across the entire limb [2]. Such organizers also operate within the insect epidermis, in which signaling centers near the borders of each segment specify cell fate [3,4]. Although the organisms favored by early experimental embryologists were well suited to the transplantation experiments that defined organizers, genetically tractable organisms are more suited to investigating the mechanisms by which organizers act.

In Drosophila, genetic screens have identified mutations in many of the genes involved in patterning the epidermis [5,6]. Genetic and molecular analyses of these segment polarity genes have revealed that two signaling molecules, encoded by wingless (wg) and hedgehog (hh), specify most of the epidermal cell fates. The wg product is a member of the evolutionarily conserved Wnt family of signaling proteins [7,8], and the hh product defines a novel class of conserved developmental signaling molecules [912,13•16•]. Recent experiments have demonstrated that not only are the molecules conserved across species, but their function is also conserved. This observation has stimulated broad interest in the action of these two proteins, as well as other segment polarity genes. In this review, we outline a new framework for thinking about the action of these patterning genes. We focus on the establishment and action of the wg- and hh-expressing cells as organizers of pattern. The other segment polarity genes fit into this framework as components of signal transduction pathways, or as factors required to maintain and accurately position the signaling centers.

Steps in segmental patterning

Early in development, a cascade of regulatory genes generates stripes of localized transcription factors that define repeating units along the body axis, called parasegments [17]. Within each parasegment, the transcription factors initiate the expression of wg in one row of cells, and the expression of both the secreted protein Hh [912] and the transcription factor Engrailed (En) in the adjacent, posterior row [1821] (Fig. 1). These two rows of cells flank the boundary between adjacent parasegments and have been identified as sources of signaling. Each row of cells signals at two different times during segmentation, with distinct outcomes [2226,27•,28••].

Fig. 1
The stabilization and fate specification phases of epidermal patterning. Cells first form at two hours after fertilization, and the epidermal cells differentiate at twelve hours (time line). Shaded bars indicate the approximate period during which signals ...

The early phase: stabilization of the signaling centers

The wg- and hh-expressing cells signal to each other, reinforcing gene expression in each cell (Fig. 1; for a comprehensive review, see [29]). It has been demonstrated that the secreted glycoprotein Wg is the ligand required for continued expression of en and hh in the neighboring cells [2224,30••]. Reciprocally, it has been proposed that the secreted protein Hh is the signal that maintains wg expression [31,32].

Several segment polarity genes act in the signal transduction pathways that operate during the stabilization phase. Although no Wg receptor has yet been identified, the genes porcupine, dishevelled, zeste-white3 and armadillo have been implicated in the sending or transduction of the wg signal (Fig. 1; [33,34•,35•,36••,37,38•40•]; review in preparation, J Klingensmith, R Nusse, personal communication). One target of this transduction cascade may be en autoactivation, and en, in turn, positively regulates hh expression [25,41].

Less is known about transduction of the putative hh signal, although some genetic evidence [42,43••] implicates the gooseberry and cubitus interruptusD transcription factors [44,45] and the fused serine/threonine kinase [46] in this pathway (Fig. 1).

At this stage in patterning, both wg and hh signaling appear to act over only short distances [30••,32]. Locally restricted signaling ensures that the domain of cells expressing either wg or hh remains narrow during development, even though the width of the parasegment grows threefold; for instance, as cell division and movements occur, some en/hh-expressing cells are displaced from the interface with wg-expressing cells and the expression of en is shut off in cells furthest away from the sustaining wg influence [47••]. Such refinement in the domains of en and wg expression is crucial for patterning, as several studies have demonstrated that widened domains of either wg or en/hh expression cause severe mis-specification of cell fates [22,23,32,48,49].

The late phase: signaling centers specify fates

The cell signaling events executed during the stabilization phase do not specify the final fate of cells, as alterations in the expression of either signal at later times dramatically affect cell fates [24,26,28••,32,48,50,51]. After the positions of the signaling centers have stabilized, however, the two signals then act to specify the distinct cell types across the parasegment [24,26,28••,50,51] (Fig. 1).

Roughly ten diverse epidermal cell types are generated within each parasegment. The final fate adopted by a cell is visualized at differentiation when the cytoskeleton distorts cells into distinct shapes (Fig. 2) [52]. Each cell then secretes a cuticular covering that indelibly reflects its shape change; therefore, cellular identity is easily visualized in the stereotyped pattern of segmentally repeated cuticular features [53].

Fig. 2
Epidermal cell fate. The photograph shows a portion of the ventral epidermis within a parasegment (anterior to the left, posterior to the right). Cell bodies are below the plane of focus. Six rows of cuticular protrusions, called denticles, are indicated, ...

The wg input is necessary for several rows of cells anterior to the wg-expressing cells to adopt their normal smooth cell fate [24,50]. The wg product also signals in the posterior direction, but in this case its effect is local. Two rows of cells posterior to the wg-expressing cells express en/hh. The most posterior row of en/hh-expressing cells adopts a denticle fate. However, wg input to the more anterior row, instructs those cells to adopt the smooth fate (Fig. 2) [26].

The hh gene appears to signal many of the remaining cell fates across the parasegment [28••]. In the dorsal epidermis, Hh acts as a morphogen in executing this role, whereas ventrally, Hh may cooperate with an unidentified signal from the en-expressing cells ([54••]; S DiNardo, unpublished data).

Uncoupling stabilization from fate specification

The mutual dependence of wg and hh expression during the stabilization phase initially masked their later, separate roles in fate specification. The standard genetic approach by which to uncover a role for a gene is to remove gene function and analyze the consequences on cell fate specification. If this gene is required for the expression of another signaling molecule, however, it is difficult to determine which ligand is responsible for which fate changes; for example, any of the changes in cell fate observed in a wg mutant could be attributed to loss of direct action of Wg, to subsequent loss of Hh activity, or to combined loss of both Hh and Wg activity. Two kinds of experiment have enabled researchers to distinguish between these possibilities and have led to the above proposal that wg and hh signaling centers operate as the two organizers of segmental pattern.

First, a temperature-sensitive allele of wg made it possible to inactivate wg at various times during development [24,28••,50,55]. Loss of Wg activity during the later, fate specification phase no longer affects the continued expression of en or of the hh signal [24,25]. This has provided a way in which to analyze the contribution of wg to fate specification without affecting the fates specified by hh signaling. Second, a key role for hh has been uncovered through experiments that bypass the stabilization phase, maintaining the expression of hh in the absence of any wg input. In this manner, we have discovered that hh can organize substantial pattern in the dorsal epidermis independently of wg signaling [28••].

The difficulty in identifying the separate early and late roles for wg and hh illustrates a general problem concerning all segment polarity genes. Does a given gene act early, during stabilization, or does it act both early and later, during fate specification?

Components of the wg signal transduction pathway act both early and late. These genes were first identified through their action during the stabilization phase, in which the target is en gene expression. However, the same signal transduction cassette mediates wg signaling during limb and wing patterning [35•,37,38•40•], even though the target is not en expression. Thus, it is likely that porcupine, dishevelled, zeste-white3, and armadillo mediate wg function during fate specification also (Fig. 1).

In contrast to this, the segment polarity genes implicated in transduction of the hh signal act only early. In embryos lacking gooseberry, cubitus interruptusD or fused, wg expression is lost, but hh-dependent cell types are still specified ([42,56]; J Heemskerk, S DiNardo, PH O’Farrell, unpublished data). Therefore, these three genes act only during early hh signaling, when hh is needed for the maintenance of wg expression, and are not required in the hh pathway for signaling cell fates.

Particular segment polarity genes position the signaling centers

Segment polarity mutants that result in mis-specification of some cell fates were first thought to define genes involved directly in the specification of the affected cell types. Genes in this class include naked, patched, and costal-2. We argue that mutations in these genes affect cell fates only indirectly and do so because they change the distribution of the important signaling molecules Wg and Hh. The changes in Wg and Hh expression precede cell fate specification [22,23], and the ultimate changes in cell fate can be explained by the altered positions of the two signaling centers, or the distance over which they now act ([26]; J Heemskerk, S DiNardo, PH O’Farrell, unpublished data). One test of this hypothesis is to determine whether the cell fates missing in a mutant background can be restored by manipulating organizer function without restoring the missing gene product; for example, naked mutants mis-express wg and lack several cell types [22,26], but if wg function is inactivated after its mis-expression, but prior to final fate specification, the missing cell types are restored [26]. Therefore, naked activity is not required for the fates of these cells, but, rather, for the control of where wg is expressed. Analogous experiments have not yet been carried out for patched and costal-2. Nevertheless, as mutations in these genes also change the position of the organizers, we postulate that these genes do not act directly in establishing cell fate, but, rather, constitute a genetic circuit that assures the accurate positioning of the signaling centers during the stabilization phase (Fig. 2). The patched and naked genes may execute this role by modulating the transduction of either the wg or hh signal during stabilization, perhaps by encoding components of the signal transduction apparatus itself [31,32,54••]. It is presently less clear how costal-2 acts to modify patterns of wg and hh expression. The view that these genes do not specify cell fate directly contrasts with a recent proposal by Bejsovec and Wieschaus [54••].

Conclusions

The wg and hh genes act as organizers of epidermal pattern. Most other segment polarity genes fit into this framework as components of the signal transduction apparatus, or as factors required to maintain or accurately position these signaling centers. Thus, few if any of the other segment polarity genes act specifically in signaling final cell fate; instead, most act in the feedback between the adjacent signaling centers.

Reinforcement is a general property of organizers

The mutual reinforcing signals that occur during the stabilization phase may be a general feature of organizers. In vertebrates, cell signaling interactions appear to sustain organizers; for instance, in the limb bud, feedback from the apical ectodermal ridge is required for the maintenance of the ZPA [57]. Recent analysis strongly suggests that the signaling molecule in the apical ectodermal ridge is fibroblast growth factor 4 [57,58], whereas the vertebrate hh homolog (vhh) encodes a ZPA signal [13•]. Although such feedback will maintain an organizer, it may also serve a larger purpose. Neighboring organizers that rely on mutually reinforcing signals would remain highly localized during growth and proliferation. This would constrain each organizer from inappropriately extending its influence and thereby disrupting overall pattern.

Distinct responses to the same signal

Early wg input stabilizes en and hh expression, whereas the later input specifies the smooth cell fate. It appears that the same components transduce the wg signal at both times. At present, we do not understand how the same transduction pathway leads to different read-outs from the responding cell. The same issue is unresolved in other inductive cell signaling processes used during development. For instance, the activation of most receptor tyrosine kinases leads to the same intracellular cascade of Ras→Raf→mitogen-activated protein (MAP) kinase interactions, yet the response of the cell differs depending on the tissue type being patterned (reviewed in [59]). In the fly epidermis, either there are novel components in the wg transduction pathway yet to be identified, or the available targets in the responding en/hh cell must be different at the two times. Perhaps a solution will be found by focusing on the fate specification phase, in which more components the wg pathway need to be identified.

Acknowledgements

Work in the lab of S DiNardo is supported by ACS grant #DB-51 and NIH grant #GM45747. Work in the lab of PH O’Farrell is supported by the NSF. S Dougan is supported by NIH pre-doctoral training grant #GM07982.

Apologies to PA Lawrence for the partially borrowed title.

Abbreviations

en
engrailed
hh
hedgehog
wg
wingless
ZPA
zone of polarizing activity

Contributor Information

Stephen DiNardo, The Rockefeller University, 1230 York Avenue, New York, New York 10021-6399, USA.

Jill Heemskerk, Department of Physiology, Columbia University College of Physicians and Surgeons, 630 West 168th Street, New York, New York 10032, USA.

Scott Dougan, The Rockefeller University, 1230 York Avenue, New York, New York 10021-6399, USA.

Patrick H O’Farrell, Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco 94143, USA.

References and recommended reading

Papers of particular interest, published within the annual period of review, have been highlighted as:

• of special interest

• of outstanding interest

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