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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
IEEE NIH Life Sci Syst Appl Workshop. Author manuscript; available in PMC 2010 May 17.
Published in final edited form as:
IEEE NIH Life Sci Syst Appl Workshop. 2009 April 9; 2009: 124–127.
doi: 10.1109/LISSA.2009.4906725

Fig. 3

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(A) Fluorescence images of dual-labeled pRNA in the complex, with the excitation for Cy3 only. (B) Typical time traces of fluorescence intensities of Cy3 and Cy5 FRET pair. ID: Intensity of donor without acceptor; IDA: Intensity of donor with acceptor; IAD: Intensity of acceptor due to FRET; IDleak: Intensity of donor leakage to acceptor channel; I0Cy3, I0Cy5: Baseline intensities for Cy3, Cy5.

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