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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Mol Neurosci. Author manuscript; available in PMC 2010 May 17.
Published in final edited form as:
PMCID: PMC2871704
NIHMSID: NIHMS201367

Functional Distribution of Nicotinic Receptors in CA3 Region of the Hippocampus

Abstract

Nicotinic acetylcholine receptor (nAChR) modulation of a number of parameters of synaptic signaling in the brain has been demonstrated. It is likely that effects of nicotine are due to its ability to modulate network excitability as a whole. A pre-requisite to understanding the effects of nicotine on network properties is the elucidation of functional receptors. We have examined the distribution of functional nAChRs in the dentate gyrus granule cells and the CA3 region of the mammalian hippocampus using calcium imaging from acute slices. Our results demonstrate the presence of functional nAChRs containing the α7 subunit (α7-nAChRs) on mossy fiber boutons, CA3 pyramidal cells, and on astrocytes. In addition, both CA3 interneurons and granule cells show nicotinic signals. Our study suggests that functional nicotinic receptors are widespread in their distribution and that calcium imaging might be an effective technique to examine locations of these receptors in the mammalian brain.

Keywords: nAChRs, CA3 pyramidal neurons, Granule cells, Nicotinic, Hippocampus

Introduction

Nicotine, an addictive substance and a key component of cigarette smoke, places a tremendous burden on healthcare systems. The drug has also been shown to positively modulate a number of brain functions linked to cognition. It improves attention, arousal, learning, learnt discrimination, and memory functions (Karczmar 1993; Narahashi et al. 2000;Rezvani and Levin 2001). The action of the drug is due to its ability to activate a class of ligand-gated ion channels, the nicotinic acetylcholine receptors (nAChRs). A key subtype of this receptor class, which is widely distributed in the brain, contains the α7 gene product assembled as a pentameric homomer (α7-nAChRs). The α7-nAChRs exhibit a high permeability for calcium and effectively raise intracellular free calcium levels ([Ca]i), mainly by downstream amplification of signals (Vijayaraghavan et al. 1992; Sharma and Vijayaraghavan 2001; Sharma et al. 2008). Downstream calcium signaling is likely to be an important player in nicotine-mediated synaptic plasticity in the brain.

Here, we demonstrate that the CA3 pyramidal cells, interneurons, and dentate granule cells, exhibit calcium transients due to the activation of nAChRs. In pyramidal neurons, the signal was mainly due to the activation of α7-nAChRs. In addition, our previous data also suggest that hippocampal astrocytes and presynaptic mossy fiber boutons (MFBs) possess functional α7-nAChRs (Sharma and Vijayaraghavan 2001). Our data result in two important findings on the effects of nAChRs on hippocampal functions—(1) functional nAChRs are more widespread than previously thought, necessitating a re-examination of their effects on circuit properties, and (2) in some cases, calcium imaging might be a more sensitive detector than electrophysiology for demonstrating the distribution of functional nAChRs.

Materials and Methods

Materials

All chemicals were obtained from Sigma (St. Louis, MO, USA) or Tocris (Ellisville, MO, USA). Tetrodotoxin was purchased from EMD Biosciences (San Diego, CA, USA), and dyes were purchased from Invitrogen (Carlsbad, CA, USA).

Methods

Hippocampal slices

Parasaggital hippocampal slices, 300μm thick, were made as described previously from our lab (Sharma and Vijayaraghavan 2003; Sharma et al. 2008). Slices were prepared from 12- to 16-day-old Sprague–Dawley rats using a Leica VT1000S vibratome, or DTK-zero 1 (Dosaka, Kyoto, Japan), in accordance with institutional guidelines. Slices were then incubated in a bicarbonate based medium (aCSF) containing 120 mM NaCl, 3.5 mM KCl, 26 mM NaHCO3, 1.25 mM NaH2PO4, 10 mM glucose, 2.5 mM MgCl2, and 1 mM CaCl2, for at least an hour before the experiment.

Calcium Imaging

Loading of acute slices with the calcium-sensitive dye fura 2-AM and the acquisition of images were done as described (Sharma et al. 2008). Briefly, slices were incubated with 20μM fura 2-AM in 0.2% Cremaphor ES for 1 h before taking them for imaging. Imaging was carried out using a Zeiss Axioskop II upright microscope fitted with a CCD camera (Cooke Sensicam) and a Sutter DG IV wavelength switcher. Images were acquired using Slide-Book (Intelligent Imaging Innovations, Denver, CO, USA) and analyzed using Microcal Origin software. Neurons were identified based on location, size, and morphology. Agents were applied for the times stated using a patch pipette as described (Sharma et al. 2008).

Statistics

Statistical significance of all comparisons was evaluated by paired or independent Student t tests. Significance was taken at p<0.05.

Results and Discussion

Modulation of presynaptic glutamate release at the principal synapse in the CA3 region, that between the large MFB and the pyramidal cell, is a powerful site for nAChR-mediated alterations in synaptic plasticity in the region. Activation of presynaptic α7-nAChRs at the MFB, by 20μM nicotine or 100μM ACh in the presence of 0.5μM atropine (ACh/At), causes a dramatic burst of glutamatergic miniature excitatory postsynaptic currents (mEPSCs) recorded from the pyramidal neuron. The onset of this burst of glutamate release is abrupt, and the peak frequency rises to an average of 51-fold above that obtained under control conditions (Sharma and Vijayaraghavan 2003). The burst is initiated by influx of calcium through the α7-nAChR ion channel, and not through voltage-gated calcium channels (VGCCs). This influx then triggers the release of calcium via the activation of ER ryanodine receptors (RyRs) by calcium-induced calcium release (CICR). This mechanism forms an effective means for the amplification of α7-nAChR calcium signals.

In addition to the increased release frequencies, the nicotine-induced mEPSC amplitudes were increased as well. The peak amplitudes of mEPSCs ranged from 8 pA (the mode of basal amplitude distribution was 13 pA) to >300 pA, with the average falling at 56 pA, compared to 20 pA under control conditions (Sharma and Vijayaraghavan 2003). The large increase in mEPSC amplitude outlasted the high frequency burst and, like the frequency effect, showed an absolute requirement for ER store calcium release.

Several lines of evidence suggest that the increase in amplitude was not due to postsynaptic alteration in glutamate receptor properties. (1) Chelating postsynaptic calcium with 10 mM BAPTA did not alter the α7-nAChR effect on the mEPSCs, even though under these conditions other calcium-dependent postsynaptic changes in synaptic currents were reduced or abolished (Yeckel et al. 1999). (2) Rise times of the mEPSCs scaled linearly with amplitude, suggesting that larger amplitude responses might arise due to multiple release events slightly staggered in time (mean stagger of ~100μs). (3) Slowing release rates by conducting experiments at lowered temperatures (15°C) caused an increase in mEPSC frequencies without any change in amplitudes. This would be expected if concerted release of multiple quanta was required for the amplitude effect because slowing down release rates would dissociate multiple events that would now appear as smaller discernible individual mEPSCs. Among the few large amplitude mEPSCs observed, all had discernible inflections along their rising phase, presumably due to slowed summations of multiple events. (4) The mEPSCs arising from the MFB-CA3 synapse are identifiable because they contain both AMPA and Kainate components. Examining individual contributions of the AMPA and Kainate currents to the mEPSCs, we found that the proportion of the two were identical in both the small and large events (Sharma et al. 2008). This makes a postsynaptic mechanism for the amplitude increase less likely as this would have to arise from an identical modulation of two separate receptor classes.

However, the amplitude increase, in addition to ER store calcium release, also requires the activation of downstream calcium/calmodulin-dependent protein kinase II (CaMKII) activation (Fig. 1).

Figure 1
α7-nAChR-mediated short-term plasticity at the MFB-CA3 pyramidal neuron synapse. Agonist-dependent activation α7-nAChRs at the terminal of MFB allows calcium influx into the terminal. Incoming calcium transient activates RyRs (cyan cylinders ...

Based on our data, we conclude that nicotine, via the activation of α7-nAChRs, causes multiple quanta to be released in a concerted fashion. The MFB is a complex terminal, which forms multiple contacts with a single postsynaptic pyramidal neuron. A single bouton can have as many as 30 active zones, easily allowing for the release of multiple quanta across active zones. Depending on the location of calcium stores in these terminals, calcium release via the activation of RyRs can simultaneously increase calcium around a number of active zones making synchronous release of multiple quanta possible. Alternately, CaMKII-dependent modification of release vesicles might allow for multivesicular release, where multiple vesicles are exocytosed from a single active zone (see Fig. 1).

All the effects described above were mimicked, at physiological temperatures, by a low concentration of nicotine (0.5μM), in the range found in the blood of smokers (Henningfield et al. 1993; Sharma and Vijayaraghavan 2008).

A most unusual finding was that the nicotine-mediated mEPSC burst was sufficient to trigger a burst of action potentials (APs) from the postsynaptic neuron (Sharma and Vijayaraghavan 2003; Sharma et al. 2008); this is the first instance of synaptic transmission in the absence of an incoming presynaptic AP. This firing of the postsynaptic neuron occurs only at a time when both the increase in frequency and amplitude are maximal. This implies that the unique short-term plasticity triggered by nicotine at this synapse occurs due to a coincidence of two α7-nAChRdependent processes—calcium-dependent increase in release frequency and a calcium- and CaMKII-dependent concerted release of multiple quanta (Fig. 1).

The triggering of synaptic transmission, and consequently, changes in synaptic strength that occurs in the absence of a physiological context, suggests that nicotine effectively “hijacks” this synapse (Fig. 1). Such an effect would imply that stability of these altered synapses now depends on the presence of the drug, and withdrawal of which would lead to destabilization. Whether such an effect is universal among CNS synapses remains untested. If true, such a mechanism would be one of the mechanisms mediating addiction to the drug.

α7-nAChRs on Astrocytes

Our work also shows that functional α7-nAChRs are present in purified hippocampal astrocytes (Sharma and Vijayaraghavan 2001). While the current density in these cells is extremely low, application of either ACh/At or nicotine gave a robust calcium response, measured using the calcium-sensitive dye fluo 3. Again, in what appears to be a consistent theme in α7-nAChR signaling, influx of calcium through the receptor itself did not significantly contribute to the calcium response. Instead, signals observed by us arose from CICR via ER calcium release. In these cells, observed signals were dependent on further calcium release from IP3 receptor activation. Our results suggest a complex interplay of calcium signals triggered by α7-nAChRs on present astrocytes. This interplay results in nicotine-induced regenerative propagation of calcium transients within and across astrocytes (Sharma and Vijayaraghavan 2002). Initial experiments in acute slices also indicate the presence of α7-nAChR-mediated calcium signals from astrocytes in the CA3 region of acute slices.

Studies over the last decade have implicated astrocytes as active partners in synaptic signaling (Haydon 2001). Activation of the glial cells can synchronize local networks and regulate neuronal activity (Fellin et al. 2004). The current view is that astrocytes might act as pacemakers, controlling the balance between excitation and inhibition of networks (Fellin et al. 2006). nAChRs on these cells could, therefore, contribute to this control of network excitability. Interestingly, evidence also implicates astrocytes in mechanisms underlying drug addiction (for review, see Haydon et al. 2008). Disruption of astrocytic function alters sensitivity to drugs of abuse, such as cocaine (Bainton et al. 2005). This emerging area of research emphasizes the idea that a complete understanding of the action of drugs of abuse will require taking into account the role of glial cells as well as neurons.

Functional α7-nAChRs in CA3 Pyramidal Neurons

Responses directly relevant to the CA3-MFB circuit were measured viz. pyramidal neurons, stratum lucidum interneurons, and dentate granule cells. Acute hippocampal slices were loaded with fura 2-AM as described under “Methods”. For these experiments, slices were bathed in 10μM DNQX, 100μM APV, 10μM gabazine, and 1μM tetrodotoxin to block ionotropic glutamate receptors, GABA A receptors, and action potentials, respectively. All experiments were carried out at room temperature.

A 10-s application of 5μM nicotine elicited calcium transients from all neurons tested (Fig. 2a–c). Neither the actual concentration of the agonist at receptor sites nor the rate of washout are determinable, though evidence exists that the drug might be concentrated in the brain achieving levels that are up to 5-fold that of the serum (Ghosheh et al. 2001). However, we have previously shown that actions of 20μM nicotine can be effectively mimicked by biologically relevant concentrations (0.5μM) of nicotine at physiological temperatures (Sharma et al. 2008). The current experiments were carried out at room temperature to minimize spontaneous activity, and cells showing calcium oscillations at showing calcium oscillations at room temperature were discarded, making the demonstration of α7nAChR transients reliable.

Figure 2
Calcium responses to a 10-s application of 5μM nicotine. a Left trace Calcium transient from a CA3 pyramidal neuron. Right trace same neuron responding to a 10-s application of 75 mM KCl (HiK). b Calcium transient in response to nicotine and HiK ...

Only neurons that responded with a rapid calcium transient to a 10-s application of 75 mM KCl (HiK) at the end of each measurement were taken for analyses to ensure the exclusion of data from unhealthy neurons. The average peak calcium response, measured as the percent change (from resting fluorescence) in the ratio of emission from 340- and 380-nm excitation, was 13±1.2%, 17±1.28%, and 5.5±2.4 (mean ± SEM from pyramidal cells, interneurons, and granule cells respectively; p<0.01 pyramidal cells vs. granule cells and interneurons vs. granule cells. The interneuron and pyramidal cell responses were not statistically different from each other; p=0.6). To rule out changes in peak due to alterations in the shape and duration of the transients, a number of kinetic parameters were evaluated (Fig. 2d). The integral of the first 90 s of the calcium transient was taken as the total response from a given cell. As expected from the peak response data, only the responses from granule cells was significantly different from those elicited from the other two neuronal types (p<0.01 granule cell vs. pyramidal neurons or interneurons). The differences in nAChR-mediated calcium transients were probably not due to differences in calcium handling capacity of the neurons because both the time to peak and the decay time constant of the transients were not significantly different (Fig. 2d, p=0.2–0.6, unpaired t test). Further, none of the parameters (Peak, Area, Time to peak, Peak, and Decay time constant) measured for the HiK-induced transient were significantly different between granule cells, pyramidal neurons, and interneurons (Fig. 2d). These results suggest that the difference in the peak and total responses to exogenous nicotine must arise from direct differences in nAChR expression/signaling.

We examined the nAChR responses from CA3 pyramidal neurons in detail. A 10-s application of 100μM glutamate, in the presence of DNQX and APV, resulted in attenuation of calcium increase from pyramidal neurons. In the presence of these antagonists, responses to the exogenous glutamate were inhibited by 82±5%. Upon washout of the antagonist, the response recovered to 74±12% of the first response (n=5 neurons; p<0.01 block vs. recovery; Fig. 3a). These results demonstrate that the glutamate receptors are effectively blocked, and thus, nicotine-induced calcium changes are not due to enhancement of glutamate release and subsequent activation of postsynaptic GluRs. This would also be expected from our previous finding that the mEPSC burst requires a more prolonged application of nicotine and occurs after a delay of many seconds (Sharma and Vijayaraghavan 2003; Sharma et al. 2008).

Figure 3
CA3 pyramidal neurons express functional α7-nAChRs. a Left Image of a field of pyramidal neurons loaded with the calcium indicator fura 2. Middle trace Response of a pyramidal neuron to 100μM glutamate applied for 10 s (black trace) after ...

A second response to 5μM nicotine after a 30-min recovery period resulted in transients that were 87±14% of the first response (n=23; p=0.6). However, after a 30-min incubation with 100 nM αBTX, nicotine responses were reduced to 14.3±2.5% of the control (n=23; p<0.001 before vs. after; Fig. 3b). These results suggest that calcium responses from CA3 principal neurons, to nicotine application, arise from the activation of α7-nAChRs.

Interestingly, responses of the CA3 pyramidal cells to 5μM nicotine were different depending on whether agonist was applied for 1 or 10 s. Cells were considered responders if the change in ratio (ΔR) upon agonist application exceeded 3-fold over baseline RMS noise. While all pyramidal neurons responded to a 10-s application of the agonist, only 65% responded to the shorter application (n=25 and 19, respectively). Selecting only the responders, the peak response to a 1-s application was 53%, and the total fluorescence change (obtained by taking the integral of the first 90 s of the response) was 27% of the 10-s response (p<0.01 and p<0.0001; respectively). The half time for decay was 9.5±1.8 and 21±2.2 s for the 1- and 10-s responses (p<0.005). Whether these differences arise from better access at 10 s or longer lasting calcium response remains to be seen. The latter possibility would be consistent with the much slower transients seen with 30-s application at MFBs observed by us (Sharma and Vijayaraghavan 2003; Sharma et al. 2008), as well as the prolonged presence of nicotine required for many downstream calcium-dependent effects of α7nAChRs (Berger et al. 1998; Dajas-Bailador et al. 2000; Hu et al. 2002).

Taken together, our data suggest that functional nAChRs are present in all the components of the CA3 circuit tested so far. Previous studies have concluded that functional α7-nAChRs were absent from CA3 pyramidal neurons and dentate granule cells (Sharma and Vijayaraghavan 2003; the CA3 circuit tested so far). Previous studies have concluded that functional α7-nAChRs were absent from CA3 pyramidal neurons and dentate granule cells (Sharma and Vijayaraghavan 2003; Frazier et al. 2003; Sharma et al. 2008). In the CA1 region of the hippocampus, either no (Khiroug et al. 2003) or small (Ji et al. 2001) responses have been reported from pyramidal neurons consistent with our electrophysiological data obtained with regard to the CA3 pyramidal neurons. At the same time, α7-nAChR currents elicited from interneurons of the hippocampus have been demonstrated from both the CA1 and the dentate region (Klein and Yakel 2005). As rapid agonist application and washout are difficult to achieve in acute slices, a part of the difficulty in detecting small α7-nAChR currents in pyramidal neurons and granule cells might be due to the rapid desensitization of α7-nAChRs (Zhang et al. 1994). A second issue in slices is one of access and location. If the nAChRs are located on processes away from the recorded neuron or non-planar, it might be harder to detect agonistevoked currents. This remains to be addressed. The ability of calcium signals to be amplified by depolarization, followed by activation of VGCCs (Vijayaraghavan et al. 1992) as well as downstream activation of calcium release from the endoplasmic reticulum (Sharma and Vijayaraghavan 2003; Sharma et al. 2008), makes calcium imaging potentially more reliable. Indeed, our results suggest that this is a valid assumption.

α7-nAChR currents have been recorded from all hippocampal neurons maintained in dissociated cell culture (Bonfante-Cabarcas et al. 1996; Gray et al. 1996; Alkondon et al. 1996; Radcliffe et al. 1999; Zarei et al. 1999). This discrepancy between experiments in acute slices and cell culture has been interpreted as a consequence of either culture conditions or difference in developmental time courses. Stating that better access to receptors and the ability to rapidly apply agonists in cell cultures might explain part, if not all, of this discrepancy.

On the basis of our results, a complex picture of nAChR function in the hippocampal CA3 area has emerged. The widespread distribution of these receptors (Fig. 4) in the region suggests influences on the entire network. This brings up the issue of relevance and physiological function for these receptors. One interpretation of this ubiquitous distribution across cell types is that by modulating the excitability of an entire network, nAChRs serve to modulate the gain function for a network, achieving an overall increase in arousal and allowing for better discriminability of incoming information. Such a sensitivity control might underlie nAChR effects on attention and learning. The diffuse innervation of all cortical areas by basal forebrain cholinergic innervation is consistent with this idea. Evidence for this hypothesis will require a detailed examination of the mechanics of cholinergic transmission in the CNS. The availability of a transgenic mouse model where cholinergic axons are tagged with GFP to allow visualization in live slices will aid in this endeavor. This is currently being developed in our lab.

Figure 4
A putative model for nAChR control of the CA3 network. Functional nAChRs are present in granule cells (G; cyan), CA3 interneurons (Int; green), pyramidal neurons (P; red), and astrocytes (A; blue). In addition, nAChRs are also located on synaptic terminals ...

Acknowledgments

Funding was provided by the National Institute for Drug Abuse (RO1 DA 10266), the National Institute of Deafness and Communication Disorders (RO1 DC 008855), and a Scientist Development Grant from the American Heart Association (GS).

Footnotes

Proceedings of the XIII International Symposium on Cholinergic Mechanisms

References

  • Alkondon M, Rocha ES, Maelicke A, Albuquerque EX. Diversity of nicotinic acetylcholine receptors in rat brain. V. Alpha-bungarotoxin-sensitive nicotinic receptors in olfactory bulb neurons and presynaptic modulation of glutamate release. Journal of Pharmacology and Experimental Therapeutics. 1996;278:1460–1471. [PubMed]
  • Bainton RJ, Tsai LT, Schwabe T, DeSalvo M, Gaul U, Heberlein U. Moody encodes two GPCRs that regulate cocaine behaviors and blood-brain barrier permeability in Drosophila. Cell. 2005;123:145–156. [PubMed]
  • Berger F, Gage FH, Vijayaraghavan S. Nicotinic receptor-induced apoptotic cell death of hippocampal progenitor cells. Journal of Neuroscience. 1998;18:6871–6881. [PubMed]
  • Bonfante-Cabarcas R, Swanson KL, Alkondon M, Albuquerque EX. Diversity of nicotinic acetylcholine receptors in rat hippocampal neurons. IV. Regulation by external Ca++ of alpha-bungarotoxin-sensitive receptor function and of rectification induced by internal Mg++ Journal of Pharmacology and Experimental Therapeutics. 1996;277:432–444. [PubMed]
  • Dajas-Bailador FA, Lima PA, Wonnacott S. The alpha7 nicotinic acetylcholine receptor subtype mediates nicotine protection against NMDA excitotoxicity in primary hippocampal cultures through a Ca(2+) dependent mechanism. Neuropharmacology. 2000;39:2799–2807. [PubMed]
  • Fellin T, Pascual O, Gobbo S, Pozzan T, Haydon PG, Carmignoto G. Neuronal synchrony mediated by astrocytic glutamate through activation of extrasynaptic NMDA receptors. Neuron. 2004;43:729–743. [PubMed]
  • Fellin T, Pascual O, Haydon PG. Astrocytes coordinate synaptic networks: balanced excitation and inhibition. Physiology. (Bethesda.) 2006;21:208–215. [PubMed]
  • Frazier CJ, Strowbridge BW, Papke RL. Nicotinic receptors on local circuit neurons in dentate gyrus: a potential role in regulation of granule cell excitability. Journal of Neurophysiology. 2003;89:3018–3028. [PubMed]
  • Ghosheh OA, Dwoskin LP, Miller DK, Crooks PA. Accumulation of nicotine and its metabolites in rat brain after intermittent or continuous peripheral administration of [2′ (14)C]nicotine. Drug Metabolism and Disposition. 2001;29:645–651. [PubMed]
  • Gray R, Rajan AS, Radcliffe KA, Yakehiro M, Dani JA. Hippocampal synaptic transmission enhanced by low concentrations of nicotine [see comments] Nature. 1996;383:713–716. [PubMed]
  • Haydon PG. GLIA: listening and talking to the synapse. Nature Reviews, Neuroscience. 2001;2:185–193. [PubMed]
  • Haydon PG, Blendy J, Moss SJ, Rob JF. Astrocytic control of synaptic transmission and plasticity: a target for drugs of abuse? Neuropharmacology. 2008;56:83–90. [PMC free article] [PubMed]
  • Henningfield JE, Stapleton JM, Benowitz NL, Grayson RF, London ED. Higher levels of nicotine in arterial than in venous blood after cigarette smoking. Drug and Alcohol Dependence. 1993;33:23–29. [PubMed]
  • Hu M, Liu Q, Chang KT, Berg DK. Nicotinic regulation of CREB activation in hippocampal neurons by glutamatergic and nonglutamatergic pathways. Molecular and Cellular Neurosciences. 2002;21:616–625. [PubMed]
  • Ji D, Lape R, Dani JA. Timing and location of nicotinic activity enhances or depresses hippocampal synaptic plasticity. Neuron. 2001;31:131–141. [PubMed]
  • Karczmar AG. Physiological cholinergic function in the CNS. In: Aquilonius S-M, Gillberg PG, editors. Cholinergic neurotransmission: Functional and clinical aspects. Elsevier; Amsterdam: 1993. pp. 437–466.
  • Khiroug L, Giniatullin R, Klein RC, Fayuk D, Yakel JL. Functional mapping and Ca2+ regulation of nicotinic acetylcholine receptor channels in rat hippocampal CA1 neurons. Journal of Neuroscience. 2003;23:9024–9031. [PubMed]
  • Klein RC, Yakel JL. Paired-pulse potentiation of alpha7-containing nAChRs in rat hippocampal CA1 stratum radiatum interneurones. Journal of Physiology. 2005;568:881–889. [PubMed]
  • Narahashi T, Fenster CP, Quick MW, Lester RA, Marszalec W, Aistrup GL, et al. Symposium overview: mechanism of action of nicotine on neuronal acetylcholine receptors, from molecule to behavior. Toxicological Sciences. 2000;57:193–202. [PubMed]
  • Radcliffe KA, Fisher JL, Gray R, Dani JA. Nicotinic modulation of glutamate and GABA synaptic transmission of hippocampal neurons. Annals of the New York Academy of Sciences. 1999;868:591–610. [PubMed]
  • Rezvani AH, Levin ED. Cognitive effects of nicotine. Biological Psychiatry. 2001;49:258–267. [PubMed]
  • Sharma G, Vijayaraghavan S. Nicotinic cholinergic signaling in hippocampal astrocytes involves calcium-induced calcium release from intracellular stores. Proceedings of the National Academy of Sciences of the United States of America. 2001;98:4148–4153. [PubMed]
  • Sharma G, Vijayaraghavan S. Nicotinic receptor signaling in nonexcitable cells. Journal of Neurobiology. 2002;53:524–534. [PubMed]
  • Sharma G, Vijayaraghavan S. Modulation of presynaptic store calcium induces release of glutamate and postsynaptic firing. Neuron. 2003;38:929–939. [PubMed]
  • Sharma G, Vijayaraghavan S. Nicotinic receptors containing the alpha7 subunit: a model for rational drug design. Current Medicinal Chemistry. 2008;15:2921–2932. [PMC free article] [PubMed]
  • Sharma G, Grybko M, Vijayaraghavan S. Action potential-independent and nicotinic receptor-mediated concerted release of multiple quanta at hippocampal CA3-mossy fiber synapses. Journal of Neuroscience. 2008;28:2563–2575. [PMC free article] [PubMed]
  • Vijayaraghavan S, Pugh PC, Zhang ZW, Rathouz MM, Berg DK. Nicotinic receptors that bind alpha-bungarotoxin on neurons raise intracellular free Ca2+ Neuron. 1992;8:353–362. [PubMed]
  • Yeckel MF, Kapur A, Johnston D. Multiple forms of LTP in hippocampal CA3 neurons use a common postsynaptic mechanism. Nature Neuroscience. 1999;2:625–633. [PMC free article] [PubMed]
  • Zarei MM, Radcliffe KA, Chen D, Patrick JW, Dani JA. Distributions of nicotinic acetylcholine receptor alpha7 and beta2 subunits on cultured hippocampal neurons. Neuroscience. 1999;88:755–764. [PubMed]
  • Zhang ZW, Vijayaraghavan S, Berg DK. Neuronal acetylcholine receptors that bind alpha-bungarotoxin with high affinity function as ligand-gated ion channels. Neuron. 1994;12:167–177. [PubMed]