The phrase “standards of care” for diseases refers to the generally accepted method of diagnosing and/or treating and/or managing a particular disease. This section focuses on the controversy of corticosteroid use for SCI, the importance of surgical treatment, the importance of an appropriate urinary bladder management program for dogs with SCI, and reviews current viewpoints regarding standards of care for SCI animals.
To give or not to give corticosteroids?
At the time of completion of a large multicenter human trial investigating high dose methylprednisolone therapy for SCI humans [the 2nd National Acute Spinal Cord Injury Study (NASCIS)] the medical treatment standard of care for humans with SCI occurring within 8 h of injury was to administer 30 mg/kg intravenous bolus of methylprednisolone sodium succinate (methylprednisolone) followed by an additional 23 h of 5.4 mg/kg constant rate infusion of methylprednisolone (22
). Soon after the publication of the 2nd NASCIS trial, the veterinary community adopted high dose methylprednisolone as a standard of medical therapy for acute SCI dogs and cats.
A 3rd NASCIS trial, conducted in the late 1990’s, compared high-dose methylprednisolone therapy with the 21-aminosteroid, tyrilizad mesylate (tyrilizad), and found no beneficial effect of tyrilizad over methylprednisolone, but reported a beneficial effect of methylprednisolone if given within 3 h following SCI compared to the standard 8-hour window (23
). Relatively recently, scrutiny over statistical analyses and interpretation of the data obtained from the NASCIS trials have been published (24
). When physicians and human neurosurgeons have been asked why they prescribe methylprednisolone for acute SCI, it has been made clear that many do so for fear of litigation and not because they believe it has a beneficial effect on patient outcome (26
). Furthermore, 76% of physicians who deal with SCI patients in Canada now do not prescribe methylprednisolone, a finding that is reversed from a survey conducted 5 y previously (27
). Methylprednisolone is not without untoward side-effects. These deleterious effects include melena, diarrhea, vomiting, hematemesis, hematochezia, anorexia, urinary tract infection (UTI), pneumonia, and sepsis (22
A recent study examining recovery in non-ambulatory small-breed dogs that had intact pain perception caudal to the level of the SCI, and were treated by hemilaminectomy and without methylprednisolone for acute intervertbral disc herniation, demonstrated that 100% of these animals were ambulatory by 6 wk following surgical intervention (29
). This study demonstrates that even without methylprednisolone, animals will recover following decompressive surgery. This study did not, however, evaluate any potential beneficial effect in large breed dogs and in dogs having no pain perception caudal to the level of the SCI, at presentation.
As a consequence of the questionable benefits of high-dose methylprednisolone therapy in humans in the face of untoward side-effects, high dose methylprednisolone therapy has fallen out of favor as a standard of medical care for human (27
) and veterinary patients with acute SCI. Choosing to not use high-dose methylprednisolone for dogs with acute SCI may, however, be premature and is not based upon sound scientific evaluation, given that methylprednisolone has not been rigorously evaluated following naturally occurring SCI in dogs. Consequently, a blinded multi-center trial is being conducted that will, in part, evaluate methylprednisolone in dogs with acute SCI resulting from type I intervertebral disc herniation (30
Based on the same rationale for using high dose methylprednisolone for acute traumatic SCI, veterinarians have also used dexamethasone for traumatic SCI (31
). Evidence regarding the positive benefits of using dexamethasone for SCI is lacking, however. A recent, relatively large, retrospective study examining the adverse effects of dexamethasone for acute thoracolumbar intervertebral disc herniation revealed that animals that were administered dexamethasone were more likely to have complications (such as, vomiting, diarrhea, urinary tract and wound infections, pneumonia, anemia) compared to untreated animals (31
). In fact, dexamethsone-treated dogs were 3.4 times more likely to develop untoward side-effects compared to animals not treated with dexamethasone (31
). Given these results, and the fact that there is no clear evidence that dexamethasone is helpful in promoting recovery following SCI, one must weigh the potential risks to the patient against the likely benefits of administering dexamethasone. Clients should also be made aware of the potential side-effects of dexamethasone therapy prior to their providing informed consent.
Is surgery helpful for IVDD?
With respect to non-medical care of SCI patients (surgical options), the evidence is clear that decompressive surgery is beneficial at improving the likelihood of successful sensorimotor recovery following acute prolapse of the intervertebral disc. Although medical management alone may result in significant improvement in neurological signs and without recurrence in approximately 54% of non-ambulatory/positive pain perceiving dogs with intervertebral disc disease (IVDD) (32
), up to 100% of similar presenting dogs will have successful recovery following surgical decompression alone. For paraplegic dogs lacking pain perception resulting from acute intervertebral disc herniation and having undergone surgical decompression, it is expected that 78% of these animals will regain pain perception within 2 wk of injury and all of these animals will regain the ability to locomote (6
). Furthermore, 7 of 18 dogs with prolapsed intervertebral disc herniation, paraplegia, and loss of pain perception that did not recover pain perception regained locomotor ability, but did not regain fecal or urinary continence. In comparison, only 0% to 7% of dogs lacking pain perception caudal to the level of the SCI, that are treated medically will have a successful outcome (be normal or substantially improved in their sensorimotor abilities), although studies evaluating outcome of medically treated dogs lacking pain perception, caudal to the level of the SCI, are relatively sparse (33
Are bladder management programs important?
With regard to other aspects of standards of care for complications associated with dogs with SCI, there have been 2 recent studies examining urinary tract infections in dogs being treated for SCI due to IVDD (34
). Results from these studies reveal a number of factors that should be considered in the urinary management of SCI dogs. One study demonstrated that female dogs, dogs that could not ambulate or voluntarily void urine, dogs that had not been administered antimicrobials during surgery, and dogs whose body temperature fell below 35°C during surgical decompression of the spinal cord all have an increased likelihood of developing a UTI (35
). The other study revealed that the frequency of UTIs in dogs with IVDD and having an indwelling urinary catheter (42%) was not different from dogs with indwelling urinary catheters for other reasons (55%) (34
). This study did, however, reveal that the likelihood of urinary tract infection increased [odds ratio (OR) = 1.27; 95% confidence interval (CI) = 1.05–1.55] for each day that the urinary catheter was left in place, and the odds of developing a urinary tract infection were higher if antimicrobials were administered during the time that the indwelling urinary catheter was left in place [OR = 4.54; 95% CI = 1.83–11.27] (34
). Results from these studies indicate that indiscriminate antimicrobial use for animals having an indwelling catheter should be avoided, though intraoperative antimicrobials should be administered at the time of surgical decompression of the spinal cord. Furthermore, the risk factors for developing a UTI should be avoided where possible, and duration of having an indwelling urinary catheter should be minimized.
Aims of recent and current clinical trials
Several clinical trials are aimed at promoting conduction of electrical signals, replacing lost tissue, promoting neural regeneration, and rescuing tissue from secondary injury. Since the early 1990s, a variety of therapies have been investigated in dogs with naturally occurring SCI resulting from acute intervertebral disc herniation. Several of these therapies are being examined in human clinical trials.
Pathological consequences of SCI are demyelination of the remaining axons, alterations in local ionic concentrations, and mechanical disruption of axonal membranes, all of which result in conduction block of neural signals traveling within the spinal cord. Since 1991, there have been 4 canine clinical trials aimed at improving behavioral recovery by different means of improving the conduction or repairing the axonal membranes so as to improve electrical conduction within the spinal cord. The 4 strategies used include blockade of potassium channels with 4-aminopyridine, application of oscillating electrical fields, use of intravenous surfactants, and the transplantation of olfactory ensheathing glia. In addition, a recent clinical trial attempting to rescue damaged spinal cord tissue by combating secondary injury mechanisms has been reported (38
The most thoroughly investigated therapy in chronic severe spinal cord-injured dogs is 4-aminopyridine (4-AP) (39
). The rationale for using this drug is that by blocking voltage-gated potassium channels, that normally would have been “blocked” by the myelin sheath, an improvement in action potential conduction within the spinal cord would occur and sensorimotor behavior would be improved. In this phase-I trial, it was found that administration of short-acting 4-AP to dogs affected by IVDD and that were completely paralyzed, caused improvement in hind limb placing, perception of pain, and partial recovery of the cutaneous trunci reflex. These improvements were noticed in only some of the dogs and for only a few hours after administering the drug. Administration of 4-AP was associated with elevation in body temperature, increased anxiety, and/or seizures that were controlled with diazepam. Nevertheless, subsequent clinical trials in humans were conducted and recently, the results of a sustained release 4-AP correlate (Fampridine) have demonstrated a possible benefit in improving spasticity (40
). Recently, pharmacokinetics and safety of 4-AP derivatives, with presumed increased safety and untoward side-effects compared with 4-AP, were examined in dogs (41
). This study showed improved pharmacokinetic and safety profiles of 4-AP derivatives compared with 4-AP; therefore, 4-AP derivatives may prove clinically useful in dogs with SCI.
The application of oscillating electrical fields is another mode of therapy that has been examined in completely paralyzed dogs that lack pain perception due to intervertebral disc extrusion or exogenous trauma (42
). The premises behind this trial were the experimental evidence that electrical fields applied to neurons caused the neurons to sprout and regenerate and that this regeneration was associated with recovery of the cutaneous trunci reflex in SCI guinea pigs (43
). Results from this trial revealed that dogs treated with the electrical stimulator had an overall “better” combined recovery score at 6 mo post-surgery compared with control animals (42
). Much of this recovery was in the form of improved sensory ability. Nevertheless, this method of improving sensorimotor recovery has gone on to be evaluated for use in humans (45
). So far, the electrical device appears safe for use in humans (45
Another SCI therapy, having been preliminarily examined in dogs with naturally occurring SCI (IVDD only), is the use of intravenously administered surfactants (46
). The premise behind this work is that certain surfactants can fuse severed axons or repair damaged axons, thereby potentially improving sensorimotor recovery after SCI. Results of this study demonstrated that the administration of polyethylene glycol or P-188 is safe and without untoward side-effects.(46
) This study lacked a concurrently run control group and instead compared the effects of the polymers against previously published historical controls. Furthermore, all dogs in this study had been administered methylprednisolone. Nevertheless, statistical comparisons revealed that both P-188 and polyethylene glycol-treated animals had improved recovery compared to the historical control animals. Improvement in sensory ability, proprioception, and locomotion accounted for the significant difference in total neurological score between polymer-treated and historical control groups. Given that the main purpose of this study was to demonstrate safety and to be a pilot study in nature, there is a need to conduct a blinded controlled study in which the polymer is administered alone and not in conjunction with methylprednisolone. Such a study is presently underway at North Carolina State University (30
Transplantation of olfactory ensheathing glia is another approach currently being investigated for use in dogs with SCI (47
). The rationale for this approach stems from the unique ability of these cells to promote regeneration in experimentally induced injured spinal cord and foster a more hospitable environment for axonal growth, increase tissue sparing after SCI, increase tissue bridging and reduce cavity formation following experimental SCI, and improve angiogenesis within the injured spinal cord [for review see (48
)]. Olfactory ensheathing glia can be harvested from the olfactory epithelium and transplanted in the same animal thereby minimizing the likelihood of rejection (47
). Thus far, transplantation of olfactory ensheathing glia into dogs spinal cords appears to be possible and is safe (47
). Trials of transplantation of olfactory ensheathing glia for human SCI are also in progress, though many aspects of 1 of these trials have been criticized (50
) and formal results are pending from others (51
One final recent clinical trial for canine SCI has investigated the use of the antioxidant, N-acetylcysteine, a precursor to glutathione and a key antioxidant in mammalian systems. Consequently, it is thought that N-acetylcysteine can minimize secondary injury that occurs after SCI by scavenging free radicals itself or by maintaining therapeutic levels of glutathione, hence this premise forms the basis for investigating N-acetylcysteine clinically in SCI dogs. The results of the study, however, did not reveal a beneficial effect of preoperatively administered N-acetylcysteine on clinical recovery in dogs having undergone decompressive surgery for intervertebral disc herniation. In part, this negative result may have occurred because the dogs used were not completely paralyzed and without pain perception caudal to the level of the SCI, and because the method of evaluating sensorimotor recovery may not have been sensitive enough to detect differences between N-acetylcysteine-treated and saline-treated groups. It is well known that dogs with intact pain sensation caudal to the level of the SCI will go on to recover substantial sensorimotor behavior without any intervention other than surgical decompression, thus therapeutic benefit may go unnoticed if a sensitive measure of behavioral recovery is not employed (52
). Furthermore, it is well known that animals that have appropriate uninjured descending spinal pathways remaining following SCI will undergo a variety of neuroplastic changes to bring about near-normal sensorimotor capabilities (16
). Consequently, animals with intact pain perception caudal to the level of the SCI (neurologically incomplete injury) may not benefit as much from medical therapeutic intervention as those lacking pain perception caudal to the level of the SCI. Another possible reason for this study to have not found an effect of N-acetylcysteine in SCI dogs is that only 1 dosage was investigated, and this dosage failed to reduce a urinary marker of oxidative stress. Although, at first glance, it appears as though N-acetylcysteine is not beneficial for SCI in dogs, it is important that further studies investigating its use in animals lacking pain perception caudal to the level of the SCI and using sensitive and objective measures be performed, and that the pharmacokinetics of N-acetylcysteine in SCI dogs be performed so as to determine a rational therapeutic dosing regime.
In conclusion, it is generally agreed amongst those in the spinal cord injury research community that there will not be 1 solely effective therapy (a magic bullet) for SCI. Instead, effective therapy for SCI will likely involve a tailor-made multimodal approach that takes into consideration the signalment and history of the patient, as well as the etiology of the SCI. In particular, as was discussed in part I of this review, there is a need for developing robust clinical trials investigating physical therapy (especially treadmill assisted therapy) solely and in combination with other therapies for dogs and cats with SCI; afterall, it is well known that cats with experimental SCI benefit substantially from treadmill training (57