Chimpanzees (Pan troglodytes), humans' closest living relative, are not only genetically similar to humans but also share susceptibility to some infectious diseases. Because the chimpanzees are used in biomedical research, investigation of their genetic background becomes important to extrapolated results for human.
This report describes the identification of a novel Patr-B*01 allele found in a chimpanzee studied under Institutional Animal Care and Use Committee-approved protocols. The determination of nucleotide sequence of the polymorphic exons 2 and 3 of Patr-B was performed by sequences-based typing (SBT) techniques as previously described (1
). The electropherogram of directed sequencing showed the presence of two superimposed peaks in exon 2 of aligned Patr-B*0101.
To confirm the presence of two Patr-B alleles, one Patr-B*0101 and one new allele, the PCR product from two different reactions was cloned in Topo Ta Cloning Kit for sequencing (Invitrogen). Many clones for the allele of interest were obtained, from which 10 clones were sequenced. The consensus sequence has been submitted to GenBank, accession number GQ470394, and to the IPD-MHC Database receiving the official name Patr-B*0102 (2
The new allele differs from the sequence of Patr-B*0101 by a non-synonymous nucleotide exchange at first and third position in codon 57 in exon 2 (CCG → TCA) (). This substitution changes the amino acid from proline to serine (P → S) (). Codon 57 is located outside of the antigen-binding cleft (3
Figure 1 Alignment of the nucleotide sequence of exon 2 (A) and the partial deduced amino acid sequence (B) of Patr-B*0102 with that of Patr-B*0101. Dashes (−) indicate identity with the Patr-B*0101 sequence. Numbers above correspond to the codon position (more ...)