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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Pharmacol Res. Author manuscript; available in PMC 2010 May 11.
Published in final edited form as:
PMCID: PMC2867105
NIHMSID: NIHMS193920

A Role for 2-Arachidonoylglycerol and Endocannabinoid Signaling in the Locomotor Response to Novelty Induced by Olfactory Bulbectomy

Abstract

Bilateral olfactory bulbectomy (OBX) in rodents produces behavioral and neurochemical changes associated clinically with depression and schizophrenia. Most notably, OBX induces hyperlocomotion in response to the stress of exposure to a novel environment. We examined the role of the endocannabinoid system in regulating this locomotor response in OBX and sham-operated rats. In our study, OBX-induced hyperactivity was restricted to the first 3 min of the open field test, demonstrating the presence of novelty (0–3 min) and habituation (3–30 min) phases of the open field locomotor response. Levels of the endocannabinoids 2-arachidonoylglycerol (2-AG) and anandamide were decreased in the ventral striatum, a brain region deafferented by OBX, whereas cannabinoid receptor densities were unaltered. In sham-operated rats, 2-AG levels in the ventral striatum were negatively correlated with distance traveled during the novelty phase. Thus, low levels of 2-AG are reflected in a hyperactive open field response. This correlation was not observed in OBX rats. Conversely, 2-AG levels in endocannabinoid-compromised OBX rats correlated with distance traveled during the habituation phase. In OBX rats, pharmacological blockade of cannabinoid CB1 receptors with either AM251 (1 mg kg−1 i.p.) or rimonabant (1 mg kg−1 i.p.) increased distance traveled during the habituation phase. Thus, blockade of endocannabinoid signaling impairs habituation of the hyperlocomotor response in OBX, but not sham-operated, rats. By contrast, in sham-operated rats, effects of CB1 antagonism were restricted to the novelty phase. These findings suggest that dysregulation in the endocannabinoid system, and 2-AG in particular, is implicated in the hyperactive locomotor response induced by OBX. Our studies suggest that drugs that enhance 2-AG signaling, such as 2-AG degradation inhibitors, might be useful in human brain disorders modeled by OBX.

Keywords: endocannabinoid, olfactory bulbectomy, novelty, 2-arachidonoylglycerol, 2-AG, locomotor response

1. Introduction

The endocannabinoid signaling system is an emerging target for drug discovery efforts aimed at identifying pharmacotherapies for psychiatric conditions [1]. The lipid mediators 2-arachidonoylglyerol (2-AG) and anandamide are the best characterized endocannabinoids isolated to date. Endocannabinoids are endogenous cannabis-like substances that bind to cannabinoid CB1 and CB2 receptors to produce physiological effects [13]. CB1 and CB2 receptors belong to a family of Gi/o-protein-coupled receptors and engage complex signaling mechanisms [3]. Activation of CB1 receptors suppresses neuronal excitability by inhibiting voltage-gated Ca++ channels and activating inwardly rectifying K+ channels [46]. Endocannabinoids are synthesized and released on demand upon receptor-stimulated or activity-dependent cleavage of membrane phospholipid precursors [7,8]. After release, endocannabinoids activate presynaptic CB1 receptors to modulate neurotransmission in a retrograde manner [911]. Endocannabinoid signaling at CB1 receptors is thus postulated to serve as a synaptic “circuit breaker” [12] within neuronal systems that modulate responses to stress, novelty and rewarding stimuli [13,14].

Consistent with a retrograde signaling mechanism, CB1 receptors are expressed presynaptically in the striatum [1517]. Here, activation of cannabinoid CB1 receptors inhibits gamma-aminobutyric acid (GABA) release as well as K+-evoked and Ca++-dependent glutamate release. In striatum, cannabinoids also reduce basal glutamate outflow and uptake, although these phenomena are not necessarily CB1 receptor-dependent [18]. Thus, endocannabinoid signaling in the striatum modulates neural transmission at the interface between glutamatergic, GABA-ergic and dopaminergic transmission [18,19]. This circuitry is implicated in the regulation of emotion, motivation and locomotor behavior [13,14].

The olfactory bulb provides the primary glutamatergic innervation to the olfactory tubercle region of the ventral striatum. Olfactory bulbectomy (OBX) deafferents the ventral striatum, thereby altering dopaminergic regulation throughout the striatum. OBX increases striatal tyrosine hydroxylase and dopamine receptor levels, induces sprouting of dopaminergic axons [20,21] and enhances sensitivity to the catecholamine reuptake inhibitor cocaine [22]. Recent work in our laboratories documents that OBX increases striatal dopamine overflow [23] and increases locomotor responsivity to the indirect dopamine agonist amphetamine [24]. OBX thus induces changes in dopaminergic activity characterized by increased presynaptic tone and paradoxical receptor supersensitivity. Such dysregulation in ventral striatal function is reminiscent of neurochemical alterations observed in human depression and schizophrenia [2527].

Most pertinent to the present studies, OBX induces hyperlocomotion in response to the stress of exposure to a novel open field [28,29]. This phenomenon is attributable to hyperdopaminergic transmission [20]. We recently reported that OBX rats exhibit insensitivity to acute and chronic effects of URB597, an inhibitor of the anandamide-degrading enzyme fatty-acid amide hydrolase (FAAH). Specifically, in sham-operated rats, URB597 produced a CB1-dependent suppression of novelty-induced locomotor activity following acute administration. Chronic administration of URB597 also produced a CB1-dependent suppression of amphetamine-induced locomotor sensitization in sham-operated rats. However, both the acute and chronic effects of URB597 were strikingly absent in OBX rats [24]. We, therefore, hypothesized that endocannabinoids, such as anandamide, dampen the locomotor response to novelty in intact rats, and that this regulation is impaired by OBX. Thus, OBX rats should exhibit decreases in endocannabinoid accumulation in brain regions deafferented by OBX, a differential sensitivity to CB1 receptor blockade, and a characteristic hyperlocomotor response to a novel open field. In the present study, we tested the hypothesis that dysregulation of the endocannabinoid signaling system contributes to the hyperlocomotor response induced by OBX using three complementary approaches. First, we compared the impact of CB1 receptor blockade on locomotor responses to novelty in OBX and sham-operated rats. Second, we quantified endocannabinoid levels in sensorimotor and limbic brain regions deafferented by OBX using liquid chromatography-mass spectrometry (LC/MS). Third, we compared cannabinoid receptor densities in brains of OBX and sham-operated rats using binding of the potent radioligand [3H]CP55,940 and quantitative autoradiography. These latter studies were performed in the same brains that were used to correlate open field locomotor activity with endocannabinoid content in both OBX and sham-operated rats. Our studies provide direct evidence that endocannabinoids are profoundly dysregulated in a rodent model of hyperdopaminergic symptomatology. Our findings further identify the endocannabinoid system as a possible pharmacological target for the treatment of psychiatric disorders such as depression and schizophrenia.

2. Materials and Methods

2.1. Subjects and Surgical Procedures

Adult male Sprague-Dawley rats (n = 71; Harlan, Indianapolis, IN, USA) weighing 250 –300 g (8 – 10 weeks old) prior to surgery were used. The University of Georgia Animal Care and Use Committee approved all behavioral and surgical procedures. Rats were housed in groups of 2 to 5 per cage in a humidity- and temperature-controlled animal housing facility. Lights were on at 0600 and off at 1800. Behavioral testing took place during the light phase between 0830 and 1800. Rats were randomly assigned to either sham or OBX surgery and to either vehicle, rimonabant or AM251 drug treatment groups. To control for time-dependent variations in locomotor activity, the order in which rats were tested throughout the day was also random. For OBX surgery, rats (n = 34) were anesthetized with a combination of pentobarbital (65 mg kg−1 intraperitoneally (i.p.); Sigma, St. Louis, MO) and ketamine hydrochloride (100 mg kg−1 i.p.; Fort Dodge Laboratories, Fort Dodge, IA, USA) or isoflurane (Abbott Laboratories, North Chicago, IL, USA). Burr holes measuring 3 mm in diameter were bilaterally drilled approximately 5 mm anterior to bregma and 1 mm lateral to the midline. The dura mater was pierced and a curved plastic pipette tip was used to aspirate the olfactory bulbs. The resulting cavity was filled with Gelfoam (Upjohn, Kalamazoo, MI, USA). Rats receiving the sham surgery (n = 37) underwent the same procedure except that the olfactory bulbs were not aspirated. In all OBX rats, confirmation of olfactory bulb lesion was made by brain dissection at the end of the experiments. Lesions were considered complete if the bulbs were totally severed from the forebrain, the weight of the tissue dissected from the olfactory bulb cavity did not exceed 5 mg, and frontal lobes were not bilaterally damaged. Sham-operated rat brains were also examined to verify that olfactory bulbs were intact following sham surgery. Histological verifications were performed by an investigator blinded to the experimental conditions. Only the brains of drug-naïve rats were collected for post-mortem LC/MS analysis and receptor autoradiography.

2.2. Chemicals

[2H4]-Anandamide was prepared in the lab [30]. Rimonabant and [3H]CP55,940 were gifts from the National Institute on Drug Abuse. AM251 was purchased from Tocris Bioscience (Ellisville, MO, USA) or Cayman Chemical Company (Ann Arbor, MI, USA). AM251 was dissolved in a vehicle composed of dimethyl sulfoxide: cremophor: ethanol: saline vehicle (1:1:1:17 in volume). Rimonabant was dissolved in ethanol: emulphor: saline (1:1:8 in volume).

2.3. Locomotor Response to Open Field Exposure

Approximately two weeks following surgery, rats were placed individually in the center of a polycarbonate activity monitor chamber (Med Associates, St. Albans, VT, USA) measuring 44.5 × 44 × 34 cm housed in a darkened, quiet room and remained undisturbed for 30 min. A 25-watt bulb positioned one meter over the chamber provided illumination. Activity was automatically measured by computerized analysis of photobeam interrupts (Med Associates). Distance traveled in the arena was used for data analysis. Results are expressed as average distance traveled in each 3 min time bin. Locomotor responses upon exposure to the novel open field were compared in OBX (n = 10) and sham (n = 10) rats in the absence of pharmacological manipulations. The effect of CB1 receptor blockade on open field activity was also evaluated in OBX rats receiving AM251 (1 mg kg−1, n = 7), rimonabant (1 mg kg−1, n = 9), the 1:1:1:17 vehicle (n = 3) or the 1:1:8 vehicle (n = 5). Sham rats similarly received either AM251 (1 mg kg−1, n = 8), rimonabant (1 mg kg−1, n = 8), the 1:1:1:17 vehicle (n = 7) or the 1:1:8 vehicle (n = 4). All drug injections were administered i.p. in a volume of 1 mL kg−1 body weight. Antagonists were employed at low doses that fail to produce anxiogenic-like effects [31] or intrinsic effects on locomotor activity [32]. Drugs were administered 30 min prior to exposure to the novel open field. Animals remained in their home cages prior to assessment of open field activity.

2.4. Tissue Dissections

Drug-naive OBX and sham rats (n = 10 per group) were decapitated immediately following the exposure to the novel open field. Brains were rapidly dissected, divided into two hemispheres along the longitudinal axis and snap frozen in precooled isopentane (−30°C). Frozen brains were stored at low temperature (−30°C and −80°C) until use. One hemisphere was used to obtain tissue punches for endocannabinoid quantification and the other hemisphere was used to measure cannabinoid receptor density and distribution using [3H]CP55,940 binding and quantitative autoradiography.

2.5. Lipid Extractions

Punches from single-hemisphere frozen brains of drug-naive rats were homogenized in methanol (0.3 mL) containing [2H4]-anandamide and [2H8]-2-AG (Cayman Chemicals, Ann Arbor, MI, USA) as internal standards. Protein concentration was determined in the homogenate to normalize samples using the bicinchinonic acid (BCA) protein assay (Pierce, Rockford IL). Tissue was punched according to distance from bregma using the rat brain atlas of Paxinos and Watson [33] as a guide. Tissue weights for punches derived from ventral striatum (1.7 mm anterior-posterior (AP), 2 mm medial-lateral (ML), −8 mm dorsal-ventral (DV)), hippocampus (−3.6 mm AP, 1 mm ML, −3.5 mm DV), amygdala (−3.6 mm AP, 4.5 mm ML, −9 mm DV) and cerebellum (−10.04 mm AP, 0 mm ML, −5 mm DV) ranged from 3–4 mg (2 mm × 1 mm punches). Tissue weights for the piriform cortex (−1.88 mm AP, 5.5 mm ML, −9 DV, relative to bregma) ranged from 1.5–2 mg (1 mm × 1 mm punches). Lipids were extracted with chloroform (2 vol), washed with water (1 vol), and fractionated by open-bed silica gel column chromatography [34]. Briefly, the lipids were reconstituted in chloroform and loaded onto small glass columns packed with Silica Gel G (60-Å 230–400 Mesh ASTM; Whatman, Clifton, NJ, USA). Anandamide and 2-AG were eluted from the columns with 9:1 chloroform/methanol (vol/vol). Eluates were dried under N2 and reconstituted in 50 µL of methanol for LC/MS analyses.

2.6. LC/MS Analyses

An 1100-LC system coupled to a 1946D-MS detector (Agilent Technologies, Inc., Palo Alto, CA, USA) equipped with an electrospray ionization interface was used to measure anandamide and 2-AG levels in select brain regions punched from a single hemisphere of each frozen sample. Lipids were separated using a XDB Eclipse C18 column (50 × 4.6 mm i.d., 1.8 mm, Zorbax), eluted with a gradient of methanol in water (from 75% to 85% in 2.5 min and then to 90% in 7.5 min) at a flow rate of 1.0 ml/min. Column temperature was kept at 40°C. MS detection was in the positive ionization mode, capillary voltage was set at 3 kV and fragmentor voltage was varied from 120V. N2 was used as drying gas at a flow rate of 13 liters/min and a temperature of 350 °C. Nebulizer pressure was set at 60 PSI. Quantifications were conducted using an isotope-dilution method, monitoring Na+ adducts of the molecular ions ([M+Na]+). The limits of quantitation were 0.08 pmol for anandamide and 0.4 pmol for 2-AG.

2.7. Receptor Binding and Autoradiography

Single hemisphere coronal brain tissue sections (14 µM thickness) were cryostat cut and mounted four sections per slide. Cannabinoid receptor binding was performed using [3H]CP55,940 (specific activity: 77.5 Ci/mmol; Research Triangle Institute, Research Triangle Park, NC, USA) as described previously [15,35,36]. Nonspecific binding was determined in the presence of 10 µM CP55,940. Briefly, binding was performed in cytomailers (3 h at 37 °C) in 50 mM Tris-HCl (pH 7.4) containing 5% bovine serum albumin and 5 nM [3H]CP55,940. Slides were washed (4 h at 0 °C) in the same buffer containing 1% bovine serum albumin, fixed in 0.5% formalin in 50 mM Tris-HCl (pH 7.4 at 25 °C) and blown dry. Sections were apposed to [3H]-sensitive film (Amersham Hyperfilm, GE Healthcare LifeSciences, Piscataway, NJ, USA) together with [3H] standards ([3H] microscales, Amersham, Arlington Heights, IL, USA) for 14 weeks. Images were captured using a Microtek ScanMaker 9800XL scanner.

2.8. Densitometry

Densitometry was performed using the public domain NIH Image software (U.S. National Institutes of Health, http://rsb.info.nih.gov/nih-image/) on a Macintosh computer (Macintosh, Cupertino, CA, USA). The mean densities for relevant brain regions of the scanned tissue images were calculated and converted to nCi/mg tissue wet weight based on a best-fit polynomial equation calibration formula that takes into account tissue equivalent values provided by Amersham. Brain areas were outlined using the rat brain atlas [33] as a guide. The nCi/mg values for each structure in each tissue section of each animal were calculated. Densitometric measurements were determined in 3–4 sections each for total and non-specific binding for each animal. Non-specific binding, determined in near adjacent sections, was subtracted from total binding values to obtain specific binding values used in data analysis. Receptor densities were calculated within surgery groups by averaging specific binding values across animals. Densitometric measurements were performed by an experimenter blinded to the experimental condition.

2.9. Statistical Analysis

For each animal, distance traveled was averaged for each three minute bin (over 30 min), generating ten time points that were used in the analyses. Data was analyzed using SPSS statistical software (version 15, Chicago, IL, USA). Because a hallmark feature of OBX is increased locomotor activity in response to a novel open field [24,28,29], planned comparisons (one-tailed Student’s t-tests) were used to compare distance traveled by drug-naive sham-operated and OBX animals during the first 3 min bin (novelty phase) following exposure to the novel open field with type of surgery as the between-subjects variable. Separate univariate analyses of variance (ANOVA) for each surgical group were employed to compare the effects of the between-subjects variable, drug treatment, on distance traveled during the novelty phase. Fisher’s Protected Least-Significant Difference (PLSD) post hoc tests were conducted in the case of a main effect of drug treatment. To investigate the effects of surgery (in drug-naive rats) or drug treatment on the habituation phase of the locomotor response to the open field (3–30 min), distance traveled during the first 3 min of exposure to the novel open field was used as the covariate in the analysis of covariance (ANCOVA) repeated measures analyses, with time serving as the repeated measure and drug treatment serving as the between-subjects variable; main effects in this study were further investigated by separate repeated measures ANCOVAs for each drug comparison. Significant interactions between time and drug treatment were corrected with the Huynh-Feldt factor and were further analyzed with Student’s t-tests at each time point. Based on previous data from our laboratory showing that OBX animals are insensitive to an inhibitor of the anandamide-hydrolyzing enzyme FAAH [24], anandamide levels in various brain regions were compared between surgical groups using planned comparisons (one-tailed Student’s t-tests). 2-AG levels were compared between surgical groups using two-tailed t-tests. Cannabinoid receptor densities in various brain regions were compared between surgical groups using Student’s t-tests (two-tailed). Relationships between variables were analyzed using two-tailed Pearson’s r correlation. P ≤ 0.05 was considered significant.

3. Results

3.1. Open Field Activity in Drug-naive Rats

Exploratory behavior declined progressively over the 30 min observation interval following introduction of animals into the open field arena (F9, 162 = 61.84, P < 0.0001) (Figure 1a). OBX animals traveled greater cumulative distance than sham animals during the first three minutes of exposure to the novel open field (t18 = −2.28, P = 0.02, Student’s one-tailed t-test) (Figure 1b). By contrast, no difference in levels of exploratory behavior was observed between sham and OBX animals for the remainder of the observation period (3–30 min following introduction into the open field) (P = 0.847). Therefore, we divided the open field locomotor response into two phases for subsequent analyses: “novelty” (0–3 min post exposure to the novel open field) and “habituation” (3–30 min post exposure to the novel open field). Brains derived from these drug-naive animals were used to determine the impact of OBX on endocannabinoid levels and cannabinoid receptor densities in discrete brain regions.

Figure 1
Locomotor responses elicited by exposure of drug-naive rats to a novel open field consist of novelty (0–3 min) and habituation (3–30 min) phases. (a) Distance traveled in sham-operated and OBX animals decreased over the 30 min exposure ...

3.2. Control conditions

In each surgical group, distance traveled during the 30 min open field session was similar in drug-naive animals and animals that received vehicles. Therefore, these groups were pooled into a single “control” group for each surgical condition for subsequent analyses of drug effects.

3.3. CB1 Blockade with AM251and Rimonabant

During the novelty phase of the open field locomotor response, OBX controls traveled a greater distance (1110.08 ± 75.39 cm) relative to sham controls (828.3 ± 34.77 cm) (t35 = −3.46, P = 0.001, Student’s one-tailed t-test). This represented a 34% increase in total distance traveled by OBX relative to sham-operated rats during the novelty phase. Drug effects were consequently analyzed in each surgical group separately. In sham rats, AM251 (1 mg kg−1 i.p.) increased distance traveled (F2, 34 = 10.52, P = 0.00027, P < 0.007 for post hoc comparison) relative to controls and rimonabant (1 mg kg−1 i.p.) during the novelty phase (Figure 2a). Also in sham animals, rimonabant (1 mg kg−1 i.p.) decreased distance traveled relative to control rats (P = 0.01 for post hoc comparison) (Figure 2a). Assessment of a subset of the activity meter data indicated that the unexpected rimonabant-induced decrease in distance traveled exhibited by shams during the novelty phase could not be attributed to a decrease in velocity; indeed, no difference in velocity was observed between sham animals in the rimonabant and control conditions (sham control: 31.95 ± 1.37 cm/s; sham Ri: 33.33 ± 1.84 cm/s, P = 0.55, two-tailed t-test, n = 7–9/group). By contrast, OBX rats were relatively insensitive to CB1 receptor blockade during this phase; neither AM251 nor rimonabant reliably altered distance traveled (P = 0.08) (Figure 2b).

Figure 2
Blockade of CB1 receptors alters distance traveled during the novelty phase in sham rats and prevents habituation of the locomotor response to the open field in OBX rats. (a) In sham animals, AM251 (1 mg kg−1 i.p.) increased and rimonabant (1 ...

The impact of CB1 receptor blockade on the habituation phase of the open field locomotor response (3–30 min) was analyzed in each surgical group separately. Distance traveled during the novelty phase (0–3 min) was used as a covariate in these analyses. In sham rats, neither AM251 nor rimonabant affected the habituation phase of the locomotor response to the open field (P = 0.36, Figure 2c). In OBX rats, CB1 blockade increased distance traveled relative to control conditions during the habituation phase (F2, 30 = 3.43, P = 0.046). Both AM251 (1 mg kg−1) (F1, 22 = 5.21, P = 0.03) and rimonabant (1 mg kg−1) (F1, 24 = 6.59, P = 0.02) increased distance traveled throughout the entire habituation phase in OBX rats. AM251 maximally increased distance traveled during the last 6 min of the habituation phase respectively (F8,176 = 2.22, Huynh-Feldt correction, P = 0.04; P < 0.05 for each post hoc comparison; Figure 2d).

3.4. LC/MS Analyses

2-AG (t14 = 2.16, P = 0.048, two-tailed t-test) and anandamide (t14 = 1.81, P = 0.05, one-tailed t-test) levels were decreased in the ventral striatum of OBX relative to sham-operated groups (Figure 3a–c). A trend towards decreased anandamide (P = 0.09, one-tailed t-test) content in the amygdala was also observed in OBX relative to sham-operated rats (Table 1). Endocannabinoid levels in the cerebellum, piriform cortex, and hippocampus were not altered by olfactory bulbectomy (P > 0.23 for all comparisons) (Table 1, Figure 3d–f).

Figure 3
Endocannabinoid content in sham-operated and OBX rats. (a) 2-AG and (b) anandamide levels are decreased in the ventral striatum of OBX rats relative to shams. (c) Approximate location of punch and relative punch size (circle) of ventral striatal tissue ...
Table 1
Endocannabinoid content measured in brain regions of sham-operated and OBX rats

In sham-operated rats, distance traveled during the novelty phase (0–3 min) of the open field locomotor response was negatively correlated with 2-AG levels in the ventral striatum (r92 = 0.45, P = 0.05) (Figure 4a). Endocannabinoid levels did not correlate (P = 0.84 for 2-AG and P = 0.974 for anandamide) with locomotor activity observed during the novelty phase in OBX rats (Figure 4c). In OBX rats, 2-AG levels were positively correlated with distance traveled during the habituation phase (3–30 min after exposure to novelty) (r72 = 0.64, P = 0.03) (Figure 4d). Endocannabinoid levels in sham-operated rats did not correlate (P = 0.17 for 2-AG and P = 0.12 for anandamide) with locomotor activation during the habituation phase (Figure 4b). Moreover, reliable correlations between anandamide and locomotor activity were not observed in either sham-operated or OBX rats at these behaviorally relevant time points (P > 0.12 for all analyses; data not shown).

Figure 4
Endocannabinoid content is differentially correlated with distance traveled in sham-operated and OBX rats. In sham animals, 2-AG levels in the ventral striatum were (a) negatively correlated with distance traveled over the novelty phase (0–3 min) ...

3.5. Receptor Binding and Autoradiography

OBX did not reliably alter cannabinoid receptor densities in any brain region analyzed (Table 2).

Table 2
Cannabinoid receptor densities in sham-operated and OBX rats

4. Discussion

4.1. Summary of Study

OBX rats exhibit increased locomotor activity in response to a novel open field, a behavior attributed to hyperdopaminergic activity [20,23,37,38]. The OBX model has consequently been described as a model of dopaminergic dysfunction [20,21,23,26,39]. The modulatory role of endocannabinoids at the interface between glutamatergic, GABAergic and dopaminergic function was, therefore, investigated by comparing locomotor behavior, endocannabinoid levels and cannabinoid receptor densities in OBX and sham-operated rats. Our study is the first to demonstrate that OBX induces a selective depletion of endocannabinoid accumulation in the ventral striatum, a brain region deafferented by OBX. Moreover, a dysregulation of endocannabinoid signaling is specifically implicated in the hyperlocomotor response exhibited by OBX animals exposed to a novel open field.

4.2. Novelty and Habituation Phases of Exposure to a Novel Environment

In agreement with previous studies [28,29], OBX rats exhibited increased locomotor activity relative to sham rats following exposure to a novel open field. We verified and extended these observations by documenting that OBX rats exhibit this characteristic hyperlocomotor activity, under our experimental conditions, during the initial phase (0–3 min) of the open field locomotor response. OBX rats exhibit locomotor activity similar to that of shams during the remainder of the open field session (3–30 min). Thus, the locomotor response elicited by a 30-min exposure to a novel open field can be divided into a “novelty” phase (0–3 min), during which OBX rats exhibit hyperlocomotion relative to sham animals, and a “habituation” phase (3–30 min), during which OBX animals show activity levels comparable to those of sham-operated rats that decline steadily over time. These findings are in agreement with prior studies documenting that OBX-induced hyperlocomotor activity, similar in magnitude to that observed in our study, is restricted to the first few minutes of exposure to the open field [40,41]. The duration and magnitude of the hyperlocomotor response to novelty may vary due to open field conditions such as luminance and length of time that activity is monitored. In particular, Mar et al. [41] found that OBX rats habituated more quickly to the open field than sham-operated rats under low-luminance (40 W) conditions. By contrast, under high-luminance (100 W) conditions, OBX rats exhibited initially high locomotor reactivity but habituated similarly to sham-operated rats over a 5 min period. Interestingly, under our open field conditions, including luminance of 25 W, OBX rats exhibited a pattern of locomotor reactivity to the open field similar to the high luminance condition in the Mar et al. [41] study over 30 min. Thus, differences in the magnitude and duration of novelty-induced reactivity in OBX rats may be accounted for by differences in the testing apparatus, lighting conditions, length of time that activity is monitored and the method used to assess locomotor activity (i.e. distance traveled versus squares crossed). Regardless of differing experimental conditions, the hyperactive locomotor response to a novel open field is a hallmark symptom of the OBX rat (see [25] for review). Our results further support the conclusion that OBX-induced hyperactivity is a stress-related phenomenon that closely resembles psychomotor agitation, which is a prevalent symptom of schizophrenia and depression [26,42]. Thus, it is noteworthy that dysregulation in endocannabinoid signaling has been postulated to contribute to depression-like symptoms in the OBX rat and other animal models such as the forced swim-test, chronic mild and/or unpredictable stress and CB1-receptor knockout mice [4348].

4.3. Olfactory Bulbectomy Induces Ventral Striatum-specific Changes in Endocannabinoid Function

Our studies provide direct evidence that endocannabinoid content is decreased in a brain region deafferented by OBX, the ventral striatum. OBX decreased 2-AG and anandamide content in the ventral striatum, but not in other brain regions (piriform cortex, amygdala) that are known to be deafferented by OBX. This observation may reflect OBX-induced glutamatergic deafferentation of the ventral striatum and consequent loss of 2-AG biosynthesis [49] and endocannabinoid release [50]. 2-AG biosynthesis is stimulated in postsynaptic neurons through activation of postsynaptic metabotropic glutamate receptor subtype 5 in corticostriatal brain slice cultures [49]. In the striatum, activation of group I metabotropic glutamate receptors stimulates the endocannabinoid system to produce both short- and long-term changes in synaptic strength [51]. A threshold level of neuronal firing is required for postsynaptic endocannabinoid release and subsequent long-term depression of glutamatergic and GABAergic synaptic activity in the striatum [50]. Modulation of endocannabinoid content by dopamine may be secondary to changes in glutamatergic transmission [19]. The hyperdopaminergic tone induced by OBX may, therefore, lead to the observed decrease in ventral striatal endocannabinoid content in OBX rats. Anandamide and 2-AG content in the limbic forebrain is suppressed by dopaminergic agonism and increased by dopaminergic antagonism [19]. These observations also suggest that endocannabinoid release may be regulated by dopamine receptor activity. Brain regions not innervated by the olfactory bulb (e.g. cerebellum) showed no change in endocannabinoid content following OBX. Importantly, other regions known to be deafferented by OBX also showed no changes in endocannabinoid content relative to sham-operated rats. These latter observations reinforce a critical role for endocannabinoids in the ventral striatum in response to a novel open field. In our studies, endocannabinoid content was measured from brains extracted immediately after the 30 min open field exposure. The OBX-induced decrease in endocannabinoid content may, therefore, be due to loss of synaptic activity-induced mobilization of 2-AG and anandamide and/or induction of hyperdopaminergic tone in the striatum. Conditional mutant mice lacking cortical glutamatergic CB1 receptors display increased novelty-induced behavioral inhibition (decreased interaction with and approach to novel object) [52]. By contrast, those lacking cortical GABAergic CB1 receptors display increased interaction and approach to a novel object [52]. In sham rats, 2-AG may suppress the behavioral response during the novelty phase by activating CB1 receptors on GABAergic terminals of medium spiny neurons of the ventral striatum. Future studies should determine whether OBX preferentially alters endocannabinoid signaling at glutamatergic or GABAergic neurons in the striatum.

The present results suggest that endocannabinoid signaling in the ventral striatum may suppress novelty-induced hyperlocomotion in intact rats. Consistent with this interpretation, 2-AG levels are negatively correlated with locomotor activity in sham rats, suggesting that individual variability in normal endocannabinoid signaling predicts the locomotor response to a novel open field. In contrast to intact rats, endocannabinoid signaling in the ventral striatum of OBX rats is altered, as reflected by decreased endocannabinoid accumulation following open field exposure. It is, therefore, reasonable to propose that in the dysregulated state induced by OBX, the normal relationship between endocannabinoid signaling and locomotor activity no longer holds. This interpretation is supported by the finding of a modest positive correlation between 2-AG levels and locomotor activity in OBX rats. Although 2-AG levels are lower overall in the endocannabinoid-compromised OBX rats compared to controls, the positive correlation may reflect a compensatory response to the novelty stress or perhaps the hyperlocomotor behavior itself. Modest or delayed mobilization of endocannabinoids may thus occur in some OBX rats, possibly due to recruitment of cortical inputs beyond the olfactory bulb/ventral striatal circuit. However, such compensation does not achieve the levels required to suppress locomotor activity, thereby accounting for a lack of negative correlation between 2-AG and locomotion in OBX rats. Thus, in OBX rats, blockade of cannabinoid receptors with multiple CB1 antagonists increases locomotor activity only during the habituation phase of the open field locomotor response. By contrast, blockade of cannabinoid receptors with multiple CB1 antagonists had no effect on the locomotor response to the open field during the habituation phase in intact rats.

A closer examination of the different temporal patterns of associations between 2-AG levels and activity in sham and OBX rats provides further insight into the function of endocannabinoids in the ventral striatum. Specifically, in sham-operated, but not OBX animals, 2-AG content was negatively correlated with distance traveled during the novelty phase (0–3 min). In OBX, but not sham-operated animals, 2-AG content in the ventral striatum was positively correlated with distance traveled during the habituation phase (3–30 min). This correlational evidence indicates that the relationship between activity levels and 2-AG is altered by olfactory bulbectomy. It remains to be determined whether release of 2-AG is delayed or reduced in OBX rats relative to sham-operated rats in response to novelty. Future studies employing microdialysis should enable direct measurements of changes in 2-AG accumulation in ventral striatum of awake behaving animals during open field exposure.

In our study, relationships between activity and endocannabinoid levels were specific to 2-AG. Anandamide content did not correlate with activity levels in either sham-operated or OBX rats at these behaviorally relevant time points. Recent studies by our group also demonstrate that OBX animals are relatively insensitive to acute and chronic effects of URB597, an inhibitor of the anandamide-hydrolyzing enzyme FAAH [24]. Thus, FAAH inhibition may be unable to reinstate anandamide levels in endocannabinoid-compromised OBX rats to levels observed in sham-operated rats. Further work is necessary to determine whether inhibition of 2-AG deactivation in the ventral striatum is able to suppress hyperlocomotion induced by OBX. Examination of the impact of OBX on the levels and activity of enzymes that degrade anandamide and 2-AG (i.e. FAAH and monoacylglycerol lipase, respectively) would aid in further characterizing the dysregulation in the endocannabinoid signaling observed here. Finally, CB2 receptors may be functionally relevant to drug abuse and depression [53]. Given 2-AG’s high affinity for CB2 [3], a possible contribution of CB2 receptors to the locomotor response to novelty could be explored. Future studies are required to determine whether alterations in expression of CB2 receptors are also observed following OBX. Our findings, nonetheless, demonstrate that endocannabinoid signaling, and 2-AG signaling in particular, is dysregulated by OBX.

4.4. CB1 Blockade Differentially Affects Responsivity to Novelty in Sham-operated and OBX Rats

Differences between OBX and sham-operated rats, and between CB1 antagonist treatments, were revealed during the novelty phase of the open field locomotor response. As expected, OBX produced hyperactivity during the novelty phase of the open field response. The novelty phase in our study likely corresponds to the same initial interval (i.e. the first 10 min block following exposure to a novel open field) during which striatal glutamate levels are transiently elevated in OBX relative to sham-operated rats [54]. Furthermore, the novelty phase corresponds to the same time interval in which a negative correlation between locomotor activity and ventral striatal endocannabinoid content was observed in sham but not OBX animals. Thus, it is plausible that direct infusion of exogenous endocannabinoids into the ventral striatum would attenuate novelty-induced hyperactivity in OBX to sham-operated levels.

In sham rats, CB1 blockade selectively altered locomotor activity during the novelty phase without altering locomotor activity during the habituation phase. During the novelty phase, AM251 (1 mg kg−1 i.p.) increased, whereas rimonabant (1 mg kg−1 i.p.) decreased, distance traveled in sham-operated but not OBX rats. Rimonabant unexpectedly decreased distance traveled in sham rats during the novelty phase of the open field session without altering velocity of travel. Comparable doses of rimonabant are reported to block hypolocomotor effects of CB1 agonists without altering motor behavior when administered alone [32,55,56]. However, these studies did not report effects of antagonists specifically during the initial period of exposure to the open field (i.e. the novelty phase in our study), which make comparisons to the present results difficult. It is possible that rimonabant paradoxically inhibits exploratory behavior in the open field of sham-operated rats by producing an anxiogenic effect via blockade of endocannabinoid tone. Support for this hypothesis is derived from the observation that mice lacking CB1 receptors are prone to anxiety- and depression-like behaviors [47,5759]. Thus, it is noteworthy that only low doses of rimonabant and AM251, which do not typically produce anxiogenic effects [31,60] or alter open arm exploratory behavior in the elevated plus maze [31,60], were employed in our study. Differences between effects of AM251 and rimonabant may also reflect differences in cannabinoid receptor binding properties or off-target effects (for review, see [61]). For example, rimonabant and AM251 may act as agonists at a putative novel cannabinoid receptor, the G protein-coupled orphan receptor GPR55, with rimonabant being the more potent of the two [62]. This receptor is mainly localized to the striatum [63] and thus represents a possible off-target site capable of mediating unexpected effects of rimonabant on locomotor activity. Consistent with this hypothesis, opposing effects of AM251 and rimonabant on locomotor activity have been reported previously; AM251 attenuated, whereas rimonabant facilitated, amphetamine sensitization in mice [64,65]. Finally, it is particularly noteworthy that rimonabant partially antagonizes, whereas AM251 fully antagonizes, the inhibition of release and uptake of striatal glutamate by cannabinoid agonists [18]. Mechanisms underlying differences observed in effects of rimonabant and AM251 on locomotor activity during the novelty phase warrant further investigation.

OBX and sham-operated rats exhibited similar levels of locomotor activity during the habituation phase of the open field locomotor response, but differed markedly in their responses to CB1 antagonists. Blockade of endocannabinoid actions with CB1 antagonists produced a hyperactive locomotor state in OBX rats specifically during the habituation phase. In OBX rats, rimonabant and AM251 may compete more effectively for CB1 receptors in the diminished endocannabinoid environment of the ventral striatum to alter locomotor activity. CB1 antagonists may block behavioral effects associated with delayed endocannabinoid mobilization in OBX rats, thereby producing a hyperlocomotor state that is manifest as a failure of acute habituation of the locomotor response to the open field. Thus, when both endocannabinoid mobilization and signaling is impaired or blocked, a failure of stress habituation may be observed (for review see [66]). Further studies are required to determine whether the pattern of changes in locomotor behavior produced by systemic administration of antagonists is mimicked by local injection of antagonists in the ventral striatum. The ability of the FAAH inhibitor URB597 to exert CB1-mediated anxiolytic effects requires saliently aversive experimental conditions, including shifting illumination intensity and a novel experimental environment; constant light illumination and habituation to the experimental environment diminishes this effect [67]. Thus, sensitivity induced by OBX, combined with exposure to the novel open field, may induce an aversive state that delays CB1-sensitive endocannabinoid release until after the novelty phase.

4.5. Olfactory Bulbectomy as a Model of Loss of Endocannabinoid Function

In a recent study, OBX increased cannabinoid receptor density and signaling in the prefrontal cortex and central-medial amygdala [48]. These effects were attenuated by chronic administration of the selective serotonin reuptake inhibitor fluoxetine which also normalized hyperactivity in the open field relative to sham-operated rats [48]. In our study, decreases in endocannabinoid content were detected in OBX relative to sham animals under conditions in which upregulations of cannabinoid receptors were not observed. Differences in the open field locomotor response of subjects evaluated in binding studies, the level of section and the postsurgical delay evaluated may contribute to differences between the studies [48]. In this latter study, acute administration of the cannabinoid agonist Δ9-tetrahydrocannabinol attenuated hyperlocomotor activity in the open field in OBX rats to sham levels [48]. These latter observations are consistent with the increase in locomotor activity induced by the CB1 antagonist AM251 in sham animals in our study. These observations, taken together, support the hypothesis that endocannabinoids, rather than being released tonically to regulate locomotor activity, are released on demand in a stimulation-contingent fashion, possibly in response to dopamine release triggered by exposure to a novel environment. A theoretical model is proposed in Figure 5 to account for our findings and illustrate our hypothesis that OBX disrupts glutamatergic innervation of the ventral striatum, which in turn decreases endocannabinoid mobilization and modulation of synaptic activity in this brain region. The consequent behavioral phenotype is one of locomotor hyperactivity in response to novelty (see Figure 5).

Figure 5
Hypothetical model showing impact of olfactory bulbectomy on endocannabinoid levels and locomotor behavior. Olfactory bulbectomy disrupts glutamatergic projections to limbic areas including the piriform cortex, amygdala and olfactory tubercle of the ventral ...

Our data extend the results of electrophysiological and pharmacological studies which document a role for endocannabinoids in modulating basal ganglia responsivity [17,18,50]. Our results suggest that the hyperlocomotor response elicited by exposure of OBX rats to an open field specifically involves endocannabinoid dysfunction in brain regions implicated in regulating responsivity to stimuli such as novelty that enhance dopaminergic transmission [68]. The endocannabinoid signaling system thus represents a possible pharmacological target for the treatment of disorders involving dopaminergic dysfunction such as depression and schizophrenia.

Acknowledgments

Supported by DA021644, DA022478, DA022702 (to A.G.H. and D.P.) and an ARCS Foundation Scholarship and University of Georgia Dissertation Completion Assistantship (to S.A.E.).

Abbreviations

2-AG
2-arachidonoylglycerol
AEA
anandamide
ANCOVA
analysis of covariance
ANOVA
analysis of variance
AP
anterior-posterior to bregma
CB1
type 1 cannabinoid receptor
DV
dorsal-ventral to bregma
FAAH
fatty-acid amide hydrolase
GABA
gamma-aminobutyric acid
i.p.
intraperitoneal or intraperitoneally
LC/MS
liquid chromatography-mass spectrometry
ML
medial-lateral to bregma
OBX
olfactory bulbectomy/bulbectomized
Fisher’s PLSD
Protected Least-Significant Difference

Footnotes

Disclosure/Conflict of Interest

The authors declare that there is no conflict of interest.

References

1. Pacher P, Batkai S, Kunos G. The endocannabinoid system as an emerging target of pharmacotherapy. Pharmacol Rev. 2006;58:389–462. [PMC free article] [PubMed]
2. Thakur GA, Tichkule R, Bajaj S, Makriyannis A. Latest advances in cannabinoid receptor agonists. Expert Opin Ther Pat. 2009;19:1647–1673. [PubMed]
3. Di Marzo V, Bifulco M, De Petrocellis L. The endocannabinoid system and its therapeutic exploitation. Nat Rev Drug Discov. 2004;3:771–784. [PubMed]
4. Howlett AC. Cannabinoid receptor signaling. Handb Exp Pharmacol. 2005:53–79. [PubMed]
5. Mackie K, Hille B. Cannabinoids inhibit N-type calcium channels in neuroblastoma-glioma cells. Proc Natl Acad Sci U S A. 1992;89:3825–3829. [PubMed]
6. Mackie K, Lai Y, Westenbroek R, Mitchell R. Cannabinoids activate an inwardly rectifying potassium conductance and inhibit Q-type calcium currents in AtT20 cells transfected with rat brain cannabinoid receptor. J Neurosci. 1995;15:6552–6561. [PubMed]
7. Di Marzo V, Fontana A, Cadas H, Schinelli S, Cimino G, Schwartz JC, Piomelli D. Formation and inactivation of endogenous cannabinoid anandamide in central neurons. Nature. 1994;372:686–691. [PubMed]
8. Piomelli D. The endocannabinoid system: A drug discovery perspective. Curr Opin Investig Drugs. 2005;6:672–679. [PubMed]
9. Kreitzer AC, Regehr WG. Cerebellar depolarization-induced suppression of inhibition is mediated by endogenous cannabinoids. J Neurosci. 2001;21:RC174. [PubMed]
10. Ohno-Shosaku T, Maejima T, Kano M. Endogenous cannabinoids mediate retrograde signals from depolarized postsynaptic neurons to presynaptic terminals. Neuron. 2001;29:729–738. [PubMed]
11. Wilson RI, Nicoll RA. Endogenous cannabinoids mediate retrograde signalling at hippocampal synapses. Nature. 2001;410:588–592. [PubMed]
12. Katona I, Freund TF. Endocannabinoid signaling as a synaptic circuit breaker in neurological disease. Nat Med. 2008;14:923–930. [PubMed]
13. Mora F, Segovia G, Del Arco A. Glutamate-dopamine-GABA interactions in the aging basal ganglia. Brain Res Rev. 2008;58:340–353. [PubMed]
14. Spanagel R, Weiss F. The dopamine hypothesis of reward: Past and current status. Trends Neurosci. 1999;22:521–527. [PubMed]
15. Herkenham M, Lynn AB, Johnson MR, Melvin LS, de Costa BR, Rice KC. Characterization and localization of cannabinoid receptors in rat brain: A quantitative in vitro autoradiographic study. J Neurosci. 1991;11:563–583. [PubMed]
16. Hohmann AG, Herkenham M. Localization of cannabinoid CB(1) receptor mRNA in neuronal subpopulations of rat striatum: A double-label in situ hybridization study. Synapse. 2000;37:71–80. [PubMed]
17. Uchigashima M, Narushima M, Fukaya M, Katona I, Kano M, Watanabe M. Subcellular arrangement of molecules for 2-arachidonoyl-glycerol-mediated retrograde signaling and its physiological contribution to synaptic modulation in the striatum. J Neurosci. 2007;27:3663–3676. [PubMed]
18. Kofalvi A, Rodrigues RJ, Ledent C, Mackie K, Vizi ES, Cunha RA, Sperlagh B. Involvement of cannabinoid receptors in the regulation of neurotransmitter release in the rodent striatum: A combined immunochemical and pharmacological analysis. J Neurosci. 2005;25:2874–2884. [PubMed]
19. Patel S, Rademacher DJ, Hillard CJ. Differential regulation of the endocannabinoids anandamide and 2-arachidonylglycerol within the limbic forebrain by dopamine receptor activity. J Pharmacol Exp Ther. 2003;306:880–888. [PubMed]
20. Gilad GM, Reis DJ. Collateral sprouting in central mesolimbic dopamine neurons: Biochemical and immunocytochemical evidence of changes in the activity and distrubution of tyrosine hydroxylase in terminal fields and in cell bodies of A10 neurons. Brain Res. 1979;160:17–26. [PubMed]
21. Lingham RB, Gottesfeld Z. Deafferentation elicits increased dopamine-sensitive adenylate cyclase and receptor binding in the olfactory tubercle. J Neurosci. 1986;6:2208–2214. [PubMed]
22. Chambers RA, Sheehan T, Taylor JR. Locomotor sensitization to cocaine in rats with olfactory bulbectomy. Synapse. 2004;52:167–175. [PubMed]
23. Masini CV, Holmes PV, Freeman KG, Maki AC, Edwards GL. Dopamine overflow is increased in olfactory bulbectomized rats: An in vivo microdialysis study. Physiol Behav. 2004;81:111–119. [PubMed]
24. Eisenstein SA, Holmes PV, Hohmann AG. Endocannabinoid modulation of amphetamine sensitization is disrupted in a rodent model of lesion-induced dopamine dysregulation. Synapse. 2009;63:941–950. [PMC free article] [PubMed]
25. Kelly JP, Wrynn AS, Leonard BE. The olfactory bulbectomized rat as a model of depression: An update. Pharmacol Ther. 1997;74:299–316. [PubMed]
26. Primeaux SD, Wilson MA, Wilson SP, Guth AN, Lelutiu NB, Holmes PV. Herpes virus-mediated preproenkephalin gene transfer in the ventral striatum mimics behavioral changes produced by olfactory bulbectomy in rats. Brain Res. 2003;988:43–55. [PubMed]
27. Song C, Leonard BE. The olfactory bulbectomised rat as a model of depression. Neurosci Biobehav Rev. 2005;29:627–647. [PubMed]
28. Klein D, Brown TS. Exploratory behavior and spontaneous alternation in blind and anosmic rats. J Comp Physiol Psychol. 1969;68:107–110. [PubMed]
29. van Riezen H, Leonard BE. Effects of psychotropic drugs on the behavior and neurochemistry of olfactory bulbectomized rats. Pharmacol Ther. 1990;47:21–34. [PubMed]
30. Fegley D, Gaetani S, Duranti A, Tontini A, Mor M, Tarzia G, Piomelli D. Characterization of the fatty acid amide hydrolase inhibitor cyclohexyl carbamic acid 3'-carbamoyl-biphenyl-3-yl ester (URB597): Effects on anandamide and oleoylethanolamide deactivation. J Pharmacol Exp Ther. 2005;313:352–358. [PubMed]
31. Patel S, Hillard CJ. Pharmacological evaluation of cannabinoid receptor ligands in a mouse model of anxiety: Further evidence for an anxiolytic role for endogenous cannabinoid signaling. J Pharmacol Exp Ther. 2006;318:304–311. [PubMed]
32. Jarbe TU, Andrzejewski ME, DiPatrizio NV. Interactions between the CB1 receptor agonist Delta 9-THC and the CB1 receptor antagonist SR-141716 in rats: Open-field revisited. Pharmacol Biochem Behav. 2002;73:911–919. [PubMed]
33. Paxinos G, Watson C. The rat brain in stereotaxic coordinates. 4th ed. 1 v. San Diego: Academic Press; 1998. (unpaged).
34. Astarita G, Piomelli D. Lipidomic analysis of endocannabinoid metabolism in biological samples. J Chromatogr B Analyt Technol Biomed Life Sci. 2009;877:2755–2767. [PMC free article] [PubMed]
35. Hohmann AG, Briley EM, Herkenham M. Pre- and postsynaptic distribution of cannabinoid and mu opioid receptors in rat spinal cord. Brain Res. 1999;822:17–25. [PubMed]
36. Hohmann AG, Herkenham M. Regulation of cannabinoid and mu opioid receptors in rat lumbar spinal cord following neonatal capsaicin treatment. Neurosci Lett. 1998;252:13–16. [PubMed]
37. Saigusa T, Tuinstra T, Koshikawa N, Cools AR. High and low responders to novelty: Effects of a catecholamine synthesis inhibitor on novelty-induced changes in behaviour and release of accumbal dopamine. Neuroscience. 1999;88:1153–1163. [PubMed]
38. Verheij MM, de Mulder EL, De Leonibus E, van Loo KM, Cools AR. Rats that differentially respond to cocaine differ in their dopaminergic storage capacity of the nucleus accumbens. J Neurochem. 2008 [PMC free article] [PubMed]
39. Holmes PV. Olfactory bulbectomy increases prepro-enkephalin mRNA levels in the ventral striatum in rats. Neuropeptides. 1999;33:206–211. [PubMed]
40. Mar A, Spreekmeester E, Rochford J. Antidepressants preferentially enhance habituation to novelty in the olfactory bulbectomized rat. Psychopharmacology (Berl) 2000;150:52–60. [PubMed]
41. Mar A, Spreekmeester E, Rochford J. Fluoxetine-induced increases in open-field habituation in the olfactory bulbectomized rat depend on test aversiveness but not on anxiety. Pharmacol Biochem Behav. 2002;73:703–712. [PubMed]
42. Holmes PV. Rodent models of depression: Reexamining validity without anthropomorphic inference. Crit Rev Neurobiol. 2003;15:143–174. [PubMed]
43. Adamczyk P, Golda A, McCreary AC, Filip M, Przegalinski E. Activation of endocannabinoid transmission induces antidepressant-like effects in rats. J Physiol Pharmacol. 2008;59:217–228. [PubMed]
44. Bortolato M, Mangieri RA, Fu J, Kim JH, Arguello O, Duranti A, Tontini A, Mor M, Tarzia G, Piomelli D. Antidepressant-like activity of the fatty acid amide hydrolase inhibitor URB597 in a rat model of chronic mild stress. Biol Psychiatry. 2007;62:1103–1110. [PubMed]
45. Hill MN, Barr AM, Ho WS, Carrier EJ, Gorzalka BB, Hillard CJ. Electroconvulsive shock treatment differentially modulates cortical and subcortical endocannabinoid activity. J Neurochem. 2007;103:47–56. [PubMed]
46. Hill MN, Carrier EJ, McLaughlin RJ, Morrish AC, Meier SE, Hillard CJ, Gorzalka BB. Regional alterations in the endocannabinoid system in an animal model of depression: Effects of concurrent antidepressant treatment. J Neurochem. 2008;106:2322–2336. [PMC free article] [PubMed]
47. Maccarrone M, Valverde O, Barbaccia ML, Castane A, Maldonado R, Ledent C, Parmentier M, Finazzi-Agro A. Age-related changes of anandamide metabolism in CB1 cannabinoid receptor knockout mice: Correlation with behaviour. Eur J Neurosci. 2002;15:1178–1186. [PubMed]
48. Rodriguez-Gaztelumendi A, Rojo ML, Pazos A, Diaz A. Altered CB receptor-signaling in prefrontal cortex from an animal model of depression is reversed by chronic fluoxetine. J Neurochem. 2009;108:1423–1433. [PubMed]
49. Jung KM, Mangieri R, Stapleton C, Kim J, Fegley D, Wallace M, Mackie K, Piomelli D. Stimulation of endocannabinoid formation in brain slice cultures through activation of group I metabotropic glutamate receptors. Mol Pharmacol. 2005;68:1196–1202. [PubMed]
50. Adermark L, Talani G, Lovinger DM. Endocannabinoid-dependent plasticity at GABAergic and glutamatergic synapses in the striatum is regulated by synaptic activity. Eur J Neurosci. 2009;29:32–41. [PMC free article] [PubMed]
51. Kreitzer AC, Malenka RC. Dopamine modulation of state-dependent endocannabinoid release and long-term depression in the striatum. J Neurosci. 2005;25:10537–10545. [PubMed]
52. Lafenetre P, Chaouloff F, Marsicano G. Bidirectional regulation of novelty-induced behavioral inhibition by the endocannabinoid system. Neuropharmacology. 2009;57:715–721. [PubMed]
53. Onaivi ES, Ishiguro H, Gong JP, Patel S, Meozzi PA, Myers L, Perchuk A, Mora Z, Tagliaferro PA, Gardner E, Brusco A, Akinshola BE, Liu QR, Chirwa SS, Hope B, Lujilde J, Inada T, Iwasaki S, Macharia D, Teasenfitz L, Arinami T, Uhl GR. Functional expression of brain neuronal CB2 cannabinoid receptors are involved in the effects of drugs of abuse and in depression. Ann N Y Acad Sci. 2008;1139:434–449. [PMC free article] [PubMed]
54. Ho YJ, Chang YC, Liu TM, Tai MY, Wong CS, Tsai YF. Striatal glutamate release during novelty exposure-induced hyperactivity in olfactory bulbectomized rats. Neurosci Lett. 2000;287:117–120. [PubMed]
55. Navarro M, Hernandez E, Munoz RM, del Arco I, Villanua MA, Carrera MR, Rodriguez de Fonseca F. Acute administration of the CB1 cannabinoid receptor antagonist SR 141716a induces anxiety-like responses in the rat. Neuroreport. 1997;8:491–496. [PubMed]
56. Rinaldi-Carmona M, Barth F, Heaulme M, Shire D, Calandra B, Congy C, Martinez S, Maruani J, Neliat G, Caput D, et al. SR141716a, a potent and selective antagonist of the brain cannabinoid receptor. FEBS Lett. 1994;350:240–244. [PubMed]
57. Haller J, Bakos N, Szirmay M, Ledent C, Freund TF. The effects of genetic and pharmacological blockade of the CB1 cannabinoid receptor on anxiety. Eur J Neurosci. 2002;16:1395–1398. [PubMed]
58. Haller J, Varga B, Ledent C, Barna I, Freund TF. Context-dependent effects of CB1 cannabinoid gene disruption on anxiety-like and social behaviour in mice. Eur J Neurosci. 2004;19:1906–1912. [PubMed]
59. Haller J, Varga B, Ledent C, Freund TF. CB1 cannabinoid receptors mediate anxiolytic effects: Convergent genetic and pharmacological evidence with CB1-specific agents. Behav Pharmacol. 2004;15:299–304. [PubMed]
60. Arevalo C, de Miguel R, Hernandez-Tristan R. Cannabinoid effects on anxiety-related behaviours and hypothalamic neurotransmitters. Pharmacol Biochem Behav. 2001;70:123–131. [PubMed]
61. Pertwee RG. Inverse agonism and neutral antagonism at cannabinoid CB1 receptors. Life Sci. 2005;76:1307–1324. [PubMed]
62. Kapur A, Zhao P, Sharir H, Bai Y, Caron MG, Barak LS, Abood ME. Atypical responsiveness of the orphan receptor GPR55 to cannabinoid ligands. J Biol Chem. 2009;284:29817–29827. [PMC free article] [PubMed]
63. Sawzdargo M, Nguyen T, Lee DK, Lynch KR, Cheng R, Heng HH, George SR, O'Dowd BF. Identification and cloning of three novel human G protein-coupled receptor genes GPR52, PsiGPR53 and GPR55: GPR55 is extensively expressed in human brain. Brain Res Mol Brain Res. 1999;64:193–198. [PubMed]
64. Thiemann G, Di Marzo V, Molleman A, Hasenohrl RU. The CB(1) cannabinoid receptor antagonist AM251 attenuates amphetamine-induced behavioural sensitization while causing monoamine changes in nucleus accumbens and hippocampus. Pharmacol Biochem Behav. 2008;89:384–391. [PubMed]
65. Thiemann G, van der Stelt M, Petrosino S, Molleman A, Di Marzo V, Hasenohrl RU. The role of the CB1 cannabinoid receptor and its endogenous ligands, anandamide and 2-arachidonoylglycerol, in amphetamine-induced behavioural sensitization. Behav Brain Res. 2008;187:289–296. [PubMed]
66. Gorzalka BB, Hill MN, Hillard CJ. Regulation of endocannabinoid signaling by stress:Implications for stress-related affective disorders. Neurosci Biobehav Rev. 2008;32:1152–1160. [PubMed]
67. Haller J, Barna I, Barsvari B, Gyimesi Pelczer K, Yasar S, Panlilio LV, Goldberg S. Interactions between environmental aversiveness and the anxiolytic effects of enhanced cannabinoid signaling by FAAH inhibition in rats. Psychopharmacology (Berl) 2009;204:607–616. [PMC free article] [PubMed]
68. Sil'kis IG. The role of dopamine-dependent negative feedback in the hippocampus-basal ganglia-thalamus-hippocampus loop in the extinction of responses. Neurosci Behav Physiol. 2008;38:399–405. [PubMed]
69. Paxinos G, Watson C. The rat brain in stereotaxic coordinates. 5th ed. 1 v. Amsterdam; Boston: Elsevier Academic Press; 2005. (unpaged).