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The cellular effects of serotonin (5-HT), a neuromodulator with widespread influences in the central nervous system, have been investigated. Despite detailed knowledge about the molecular biology of cellular signalling, it is not possible to anticipate the responses of neuronal networks to a global action of 5-HT. Heterogeneous expression of various subtypes of serotonin receptors (5-HTR) in a variety of neurons differently equipped with cell-specific transmitter receptors and ion channel assemblies can provoke diverse cellular reactions resulting in various forms of network adjustment and, hence, motor behaviour.
Using the respiratory network as a model for reciprocal synaptic inhibition, we demonstrate that 5-HT1AR modulation primarily affects inhibition through glycinergic synapses. Potentiation of glycinergic inhibition of both excitatory and inhibitory neurons induces a functional reorganization of the network leading to a characteristic change of motor output. The changes in network operation are robust and help to overcome opiate-induced respiratory depression. Hence, 5-HT1AR activation stabilizes the rhythmicity of breathing during opiate medication of pain.
Despite detailed knowledge about the molecular processes controlling G protein-coupled receptor (GPCR)-induced modulation of neuronal excitability, we cannot predict the responses of neuronal networks to a global release of neuromodulators. This is due to a diversity of neurons that express cell-specific patterns of GPCRs targeting different membrane conductances, which induce distinct operations of the network. Even more difficult to predict is the endogenous capacity of neuronal networks to adapt to variable conditions while preserving or adjusting elementary processes, such as rhythmic burst generation, activity synchronization and pattern formation. To study such integrated processes of network modulation, it is necessary to know the principles of network architecture, identify cell-specific transmitter receptor/ion channel expression profiles and develop appropriate pharmacological tools to dissect GPCR-specific responses. In such studies of integrated network physiology, the respiratory network is a suitable model system, because it produces an ongoing oscillatory activity and a highly integrated synaptic drive that generates augmenting burst activity. Eupnoeic breathing movements also depend on the network to accurately shape firing patterns and precisely control burst termination and phase transitions (Richter & Spyer 2001). All these processes are effectively adjusted by serotonergic neurons of the Raphé nuclei, which exhibit ongoing discharges (Ballantyne et al. 2004) to continuously release serotonin (5-HT; Richter et al. 1999).
Most serotonin receptors (5-HTRs) are GPCRs operating through signalling pathways that are shared targets of other transmitters, e.g. noradrenaline, dopamine and opiates. All these GPCRs are abundantly expressed in the pre-Bötzinger complex (pre-BötC; Gray et al. 1999; Manzke et al. 2003; Viemari & Ramirez 2006), which is an essential part of the respiratory rhythm generator in the medullary brainstem of mammals (Smith et al. 1991; Pierrefiche et al. 1998; Tan et al. 2008). Signalling via most of these GPCRs converges at the shared molecular target adenylyl cyclase (AC), which regulates the formation of cellular cyclic adenosine 5′,3′-monophosphate (cAMP). This has inspired several investigations of the mechanisms of the dynamic adjustment of respiratory network function through regulation of intracellular cAMP levels (Ballanyi et al. 1997; Lalley et al. 1997; Richter et al. 1999; Bocchiaro & Feldman 2004).
An unanswered key question of clinical significance concerns the consequences of convergent signalling via serotonin receptors (5-HTRs) and μ-opioid receptors (μ-ORs). The latter are well-known pharmacological targets for anaesthesia and the treatment of pain. Clinically useful μ-OR agonists, however, depress central respiratory activity, causing cessation of rhythmic breathing movements (Mellen et al. 2003; Lalley 2006). An averting medical approach against opioid-induced apnoea is possible with 5-HT4R agonists that re-activate cellular AC (Manzke et al. 2003), but such medication is so far not useful in men because 5-HT4R agonists that permeate through the blood–brain barrier are not available for clinical practice (Lotsch et al. 2005).
In the present experiments, we focused on the convergent signalling of μ-OR and 5-HT1ARs following the observation that fentanyl-induced depression of breathing is reversed by 5-HT1AR-agonists (Sahibzada et al. 2000). This finding is unexpected, because both μ-OR and 5-HT1AR agonists lower cAMP levels and decrease neuronal excitability (Richter et al. 1999). A straightforward explanation could be that most 5-HT1AR-agonists such as the applied 8-hydroxy-dipropyl-aminotetralin (8-OH-DPAT) also activate Gs-coupled 5-HT7Rs (Hedlund et al. 2004) that might prevail over the inhibitory Gi/o–AC pathway, thus restoring intracellular cAMP levels (Hoyer et al. 2002). Alternatively, one has to search for more complex changes in network operation if this simplistic explanation is not supported.
Experiments were performed on 10 adult cats of either sex weighing 2.8–3.8 kg using procedures that have been described in detail in other published studies from this laboratory (Lalley et al. 1997; Richter et al. 1999). Briefly, cats were anaesthetized with IV pentobarbital sodium in doses sufficient to produce deep surgical anaesthesia. The initial dose was 40 mg kg−1 intraperitoneally, followed by supplemental maintenance doses of 4–8 mg kg−1 IV, as required. Animals were given atropine methyl nitrate (0.2 mg kg−1 intraperitoneally) to minimize airway fluid secretion. Surgical procedures included insertion of a catheter in a femoral artery to monitor blood pressure, and placement of catheters in both femoral veins to administer drugs. Temperature was measured rectally and maintained at 36–38°C by external heating. Immobilization was produced with gallamine triethiodide (4 mg kg−1 IV to start and 4–8 mg h−1 or more frequently) to assist mechanical stability for intracellular recording. Animals were ventilated with oxygen-enriched (60–70% O2) room air via a cannula inserted into the trachea. To obtain stable intracellular recordings, a bilateral pneumothorax was performed, and a horseshoe-shaped pressure foot was placed gently on the surface of the dorsal medulla over and near the site of microelectrode insertion. Single-electrode current (SEC) and voltage-clamp (SEVC) recording techniques were performed as described previously (Richter et al. 1996). Neurons were impaled with sharp borosilicate glass microelectrodes filled with 2 M K-methylsulphate (DC resistance 50–70 MΩ). Membrane potential was recorded in either balanced-bridge mode, or in discontinuous single-electrode current-clamp mode (SEC-05XL amplifier; npi, Tamm, Germany; bandwidth, DC −10 KHz; switching frequencies in the range of 20–40 kHz). The gain was increased to 100 µA V−1 by the use of an integrator, without affecting SEVC stability, thus improving the accuracy of the membrane potential control.
The 5-HT1AR agonist 8-OH-DPAT (Sigma-Aldrich) was dissolved in Ringer's solution and administered slowly IV to maintain recording stability. Doses were increased in 5 µg kg−1 amounts to a final concentration of 10–50 µg kg−1. The effects of 8-OH-DPAT on respiratory network rhythm were obtained from a population of 4 post-inspiratory and 12 expiratory neurons of the ventrolateral medulla.
Sprague Dawley rats of either sex (250–350 g) were anaesthetized by intraperitoneal injection of pentobarbital (60 mg kg−1). The depth of anaesthesia was tested with a forepaw pinch. In case of reflex responses, an additional dose of pentobarbital was applied (approx. one-tenth of the initial dose). The trachea and right femoral vein were canulated with polyethylene tubing to record respiratory air flow and for drug and fluid injections, respectively. The inspiratory/expiratory airflow was recorded using a MacLab-linked pressure transducer connected to the tracheal tubing. Nociceptive responses were assessed by the tail-flick (TF) response. High-intensity light sufficient to stimulate nociceptors was applied to marked spots (1 cm from the tip of the tail, four spots at an interval of 1 cm), and the TF was quantified by measurement of the latency from ‘heat on’ to the evoked withdrawal response. The average TF response latency values of three consecutive trials before drug application were used as baseline. To avoid tissue damage, the heating was stopped when the TF latency exceeded 300 per cent of control, concluding TF response abolishment. To prevent central hypoxia, the animals were insufflated with oxygen during the whole experiment and ventilated artificially with room air at low frequency (10–15 breath min−1) after opioid-induced apnoea. Artificial ventilation was immediately stopped after detection of the first signs of spontaneous breathing movements evoked by drug application. At the end of the experiments, all animals were sacrificed using an overdose of pentobarbital that produced irreversible cardiac arrest.
The experiments on the in vivo-like in situ brainstem–spinal cord preparation were performed on Sprague-Dawley rats (P22–P32) or C57BL mice (P20–P25) as described originally (Paton 1996). For isolating the in situ brainstem–spinal cord from higher brain areas, animals were deeply anaesthetized with halothane until apnoea occurred and they were unresponsive to a forepaw pinch. Animals were then decerebrated at the pre-collicular level and cerebellectomized, bisected below the diaphragm and the skin was removed. The upper body was placed in a recording chamber and perfused retrogradely via the thoracic aorta with artificial cerebrospinal fluid (ACSF, containing in mM: 1.25 MgSO4; 1.25 KH2PO4; 5 KCl; 125 NaCl; 2.5 CaCl2; 25 NaHCO3; 10 glucose, 70 Ficoll (0.1785 mM)), and gassed with carbogen (5% CO2/95% O2; pH 7.35). The perfusate was warmed to 30°C as measured in the skull base, filtered twice and re-circulated. Norcuronium-bromide at 0.5 mg 200 ml−1 was added for immobilization. The perfusion pressure was set to 45–65 mm Hg. Using a glass suction electrode, phrenic nerve discharges were recorded as an index of central respiratory rhythm. Drugs were added to the perfusate for specific pharmacological manipulation of 5-HTR and glycine receptors.
Inspiratory neurons in the pre-BötC were recorded in whole-cell mode before harvesting the cytosol for single-cell RT–PCR analysis. Transverse slices (600 µm) were cut from the caudal medulla at the level of the pre-BötC with a vibroslicer (Campden Instruments, Loughborough, UK) and stored in ACSF at room temperature (20–23°C) for at least 30 min before experiments were started. They were then transferred to a recording chamber and kept submerged by nylon fibres for mechanical stabilization. The chamber was mounted on an upright microscope (Axioscope FS; Zeiss, Oberkochen, Germany) and perfused continuously with ACSF (20–23°C) at a flow rate of 5–10 ml min−1. Whole-cell voltage-clamp recordings were obtained with a Multiclamp 700A amplifier (Axon Instruments). Patch electrodes were pulled from borosilicate glass capillaries (Biomedical Instruments, Zülpich, Germany) on a horizontal pipette puller (Zeitz-Instrumente, Munich, Germany) and filled with ‘patch solution’ containing (in mM): 125 KCl, 1 CaCl2, 2 MgCl2, 4 Na2ATP, 10 EGTA, 10 HEPES (pH adjusted to 7.2 with KOH). Patch electrodes had DC impedances ranging between 2 and 6 MΩ.
The following pharmaceutical substances were administered in in vivo cat experiments: 8-hydroxy-2-(di-N-propylamino)-tetralin hydrobromide (8-OH-DPAT; 5-HT1AR agonist; Tocris), buspirone (5-HT1AR agonist; Sigma-Aldrich), WAY 100635 (5-HT1AR antagonist; Sigma-Aldrich), 5-carboxamidotryptamine maleate (5-CT; 5-HT7R agonist; Tocris), SB 269970 hydrochloride (5-HT7R antagonist; Tocris), strychnine hydrochloride (glycine receptors antagonist; Sigma-Aldrich), fentanyl citrate salt (μ-OR agonist; Sigma-Aldrich). In the in vivo rat experiments, the antagonists WAY 100635, SB 269970 and naloxone were used at a concentration of 1 mg kg−1, whereas 8-OH-DPAT was given at 100 µg kg−1 and fentanyl at 10–15 µg kg−1. In the perfused brainstem–spinal cord preparations, 8-OH-DPAT, WAY 100635 and SB 269970 were applied in 5–20 µM concentrations; buspirone, 20–40 µM and 5-CT, 1 µM. Fentanyl was given at a concentration of 10–30 nM and strychnine at 0.4 µM.
Since 5-HT7Rs show a high amino acid sequence identity in mouse, rat and man, we used Syrian hamsters (Charles River Laboratories), which are evolutionarily more distant from these species, to raise monoclonal 5-HT7R antibodies. Five Syrian hamsters were immunized with a 14mer peptide derived from the TM5–TM6 intracellular loop of the rat 5-HT7R (NH2-FPRVQPESVISLNG-COOH; National Center for Biotechnology Information, http://www.ncbi.nlm.nih.gov/, Accession No.: NP_075227). For immunization purposes, peptides were coupled to keyhole limpet haemocyanin (KLH) using 4-(N-maleimido-methyl)-cyclohexane-1-carboxylic acid N-hydroxy-succinimide ester. Syrian hamsters were injected subcutaneously with 150 µg KLH-coupled peptide in TiterMax Gold (Sigma, Deisenhofen, Germany) six times over a 28-day interval, followed by two intraperitoneal injections over a 3-day interval of 100 µg antigen. Three days after the last injection, the spleen was removed. The fusion of hamster spleen cells with P3X63Ag184.108.40.206 mouse myeloma cells was performed using polyethylene glycol (PEG4000; Sigma) according to standard protocols. First screenings of mouse-hamster heterohybridoma supernatants to identify peptide-binding antibodies were performed by enzyme-linked immunosorbent assay (ELISA) with solid phase-coated peptide. After isotype characterization using sandwich-ELISA procedure (clone 5HTR7/220; immunoglobulin class: IgG, light chains: λ-type), the antibodies were purified on a protein A-sepharose column with the ‘high-salt’ method according to standard protocols. The efficiency of purification was tested by Coomassie blue staining of SDS–PAGE gels containing the separated IgG molecules. Under reducing conditions, only the heavy (55 kDa) and light chains (25 kDa) of the IgG molecules were detected.
SDS–PAGE and subsequent immunoblot analysis were performed according to standard protocols. In brief, 150 µg total protein from membrane fractions of Triton X-114-extracted brainstem lysates was boiled in SDS sample buffer under reducing conditions for 5 min. Proteins were separated using a 12 per cent SDS–PAGE and transferred onto a nitrocellulose membrane by electroblotting, with a constant current of 150 mA for 2 h in a semi-dry transfer chamber. The efficacy of protein transfer was tested with both Ponceau staining of the membrane and Coomassie blue staining of the remaining protein in the gel. The membrane was blocked with 2 per cent (w/v) bovine serum albumin (BSA) for 30 min at room temperature and incubated with 5HTR7/220 antibodies at a concentration of 2 µg ml−1 for 2 h at room temperature (RT). The secondary horseradish peroxidase-conjugated goat anti-hamster IgG (Dianova, Hamburg, Germany) was used at a dilution of 1 : 4000 for 2 h at RT. Visualization of the antigen–antibody reaction was achieved with diaminobenzidine (Sigma) and hydrogen peroxide. The colour reaction was terminated with TBS/0.05 per cent (v/v) TWEEN 20. Finally, the membrane was air-dried and photographed.
Juvenile rats (P28–P32) were deeply anaesthetized with isoflurane (1-chloro-2,2,2-trifluoroethyl-difluoromethylether; Abbott, Wiesbaden, Germany). The animals were transcardially perfused with 50 ml 0.9 per cent sodium chloride followed by 200 ml 4 per cent phosphate-buffered formaldehyde. The brainstem and spinal cord were removed and post-fixed for 4 h and then cryoprotected with 30 per cent sucrose. Series of 20 and 40 µm thick transverse brainstem sections were cut using a cryostat (Frigocut, Reichert-Jung, Germany).
The following primary antibodies were diluted (1–5 µg ml−1) in a solution containing 2 per cent (w/v) BSA in phosphate-buffered saline (PBS) and applied for 48–72 h at 4°C: affinity-purified hamster monoclonal antibody against 5-HT7R (clone 5HTR7/220), guinea pig polyclonal antibody against 5-HT1AR (AB5406; Millipore), rabbit polyclonal GlyT2 antibody (G8168-21; US Biologicals) and goat polyclonal antibody against μ-OR (sc-7488; Santa Cruz), goat polyclonal antibody against choline acetyltransferase (Millipore) and guinea pig polyclonal antibody against NK-1R (AB15810; Chemicon). After incubation, the sections were rinsed three times in PBS and subsequently incubated for 4 h in 2 per cent (w/v) BSA in PBS containing species-specific secondary antibodies conjugated with Cy2, Cy3, or Cy5 (Dianova) at a dilution of 1 : 500. The analysis of the neuronal immunofluorescence was performed with a confocal laser-scanning microscope LSM 510 Meta (Zeiss, Göttingen, Germany). For data acquisition and analysis of the confocal images, we used the Lsm 510 Meta software (Zeiss, Germany). Subsequent imaging procedures (cell counting) were performed using ImageJ (http://rsb.info.nih.gov/ij/).
For real-time RT–PCR analysis, we dissected the pre-BötC that was identified by the appearance of the principal nucleus of the inferior olive from corresponding cryostat sections cut in a rostro-caudal extension of 300 µm (n = 3, two patches from both sides of three juvenile Sprague-Dawley rats, P30). Total RNA was isolated using the Trizol method (Invitrogen) according to manufacturer's instructions and quantified using a nanodrop ND-1000 spectrophotometer. RNA quality and integrity was assessed by electrophoresis using an RNA 6000 LabChip kit and an Agilent 2100 Bioanalyzer. First-strand cDNA was synthesized using the iScript cDNA Synthesis Kit (BioRad). Primer pairs were designed using the Primer3 program (http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi): hypoxanthine guanine phosphoribosyl transferase (NM_012583, 135 bp; Hprt-F: 5′-gtcaagcagtacagccccaaaatg-3′, Hprt-R: 5′-gtcaagggcatatccaacaacaaac-3′), 5-HT1AR (NM_012585, 161 bp, Htr1a-F: 5′-cacggctacaccatctactc-3′, Htr1a-R: 5′-gacgaagttcctaagctggt-3′), 5-HT7R (NM_022938, 193 bp, Htr7-F: 5′-tgcaaactctctcattaatcct-3′, Htr7-R: 5′-ggtcagagttttgtcttacagc-3′), GlyT2 (NM_203334, 210 bp, Slc6a5-F: 5′-atgatgctggcactggctggctta-3′, Slc6a5-R: 5′-agacacaaaagaagcaaacaggt-3′) and µ-OR (NM_013071, 208 bp, Oprm1-F: 5′-gtggtcgtggctgtatttat-3′, Oprm1-R: 5′-ggatgcagaactctctgaag-3′). Gel electrophoresis revealed a single PCR product, and melting curve analysis showed a single peak for all amplification products. As an additional control, all PCR products were sequenced to confirm the identity of each amplicon. Ten-fold serial dilutions generated from pooled cDNA from all samples were used as a reference for the standard curve calculation to determine primer efficiency. Triplicates of all real-time PCR reactions were performed in 25 µl reactions containing 1/20 dilution of the sample cDNA preparation from 250 ng total RNA, 400 nM of each primer, and 1 × iQ-SYBR Green Supermix (BioRad Laboratories, Ltd). The PCR reactions were cycled as follows: initial denaturation at 95°C for 10 min, 40 cycles of (denaturation 95°C/15 s, annealing 55°C/15 s, extension 72°C/30 s) and a final gradual increase of 0.5°C in temperature from 55°C to 95°C. All real-time quantifications were performed with the iCycler iQ system (BioRad) and were analysed by using a two-tailed paired t-test following normalization to the Hprt control.
To harvest cytosol for RT–PCR analysis, cells were patched and identified as inspiratory neurons. Patch pipettes were filled with internal solution containing (in mM): 120 K+-gluconate, 15 NaCl, 2 MgCl2, 10 HEPES, 0.5 Na2ATP, 1 CaCl2, 3 1,2-bis-(2-aminophenoxy)ethane-N, N, N′,N′-tetraacetic acid, pH 7.4 adjusted with KOH; solution osmolarity ranged from 285 to 290 mOsm. To allow a diffusional exchange of cytosol and pipette solution, cells were held in the whole-cell configuration for at least 15 min. Subsequently, the cytoplasm was aspirated under visual control, while the access resistance was monitored to verify intact gigaseal formation. After breaking the gigaseal, the pipette was immediately withdrawn from the slice. Reverse transcription was carried out for 10 min at 25°C followed by incubation for 50 min at 42°C (SuperScript II reverse transcriptase; Invitrogen). The PCR amplification was performed with specific primers. All primers were tested on plasmid (5-HT1A and 5-HT7 receptors) as well as on total brain cDNA (5-HT1A, 5-HT7 and μ-ORs). The primer concentration for all amplification reactions was 0.2 µM. The cycling conditions for the amplification with AmpliTag Gold polymerase (Applied Biosystems) were a hot start at 95°C for 10 min, 40 cycles at 94°C for 30 s, 56°C for 30 s, 72°C for 1 min and an elongation step at 72°C for 10 min. In parallel, PCR was also performed with the AccuPrime Taq DNA polymerase system (Invitrogen), 94°C for 2 min followed by 39 cycles denaturation (94°C for 30 s), annealing (55°C for 30 s) and elongation (68°C for 1 min). The following primer pairs were used for the specific amplification of 5-HT1AR (Htr1a-F: 5′-tctgcccagcgaggctggtc-3′ and Htr1a-R: 5′-gatcctgtagcctcgactg-3′), 5-HT7R (Htr7-F: 5′-gagtcgagaaagttgtgatcggctcca-3′ and Htr7-R: 5′-aggtacctgtcaatgctgatcacgcac-3′), μ-OR (Oprm1-F: 5′-ttctgcattgctttgggttacacg-3′ and Oprm1-R: 5′-ctgacagcaacctgattccacgta-3′), and β-actin (Actb-F: 5′tggccttagggttgcaggggg-3′ and Actb-R: 5′-gtgggccgctctaggcacca-3′).
All statistical tests were performed with repeated ANOVA followed by a Fisher LSD post hoc test using Systat 8 software (SPSS Inc., Chicago, IL, USA).
Single-cell RT-PCR analysis of the cytosol harvested from identified inspiratory neurons (figure 1a) in the brainstem slice preparation of rat revealed the presence of μ-OR, 5-HT1AR and 5-HT7R transcripts (figure 1b). The expression levels related to the housekeeping gene Hprt of the two 5-HTR transcripts were similar (5-HT1AR, 0.99 ± 0.22; 5-HT7R, 1.14 ± 0.17), and significantly lower for the neuronal glycine transporter GlyT2 (0.43 ± 0.07).
Expression patterns of 5-HT1AR, 5-HT7R and GlyT2 in the pre-BötC of rat. (a) Whole cell recording in current-clamp mode from an identified respiratory pre-BötC neuron discharging in synchrony with integrated hypoglossal nerve (XIIa) activity ...
At the protein level, we studied the cellular expression patterns of 5-HT1AR and 5-HT7R. A novel anti-peptide monoclonal antibody recognizing 5-HT7R was developed using Phe281–Gly294 (NH2-FPRVQPESVISLNG-COOH) in the third intracellular loop of the rat 5-HT7R as an antigen for immunization (figure 1c). This antibody was used in combination with commercially available antibodies recognizing 5-HT1AR and GlyT2, the neuronal glycine transporter, for specific labelling of inhibitory glycinergic neurons (figure 1e, f). To obtain a complete overview of receptor expression profiles in excitatory and inhibitory neurons, immunohistochemistry was performed using sections encompassing the entire 200 µm extension of the pre-BötC (including five consecutive 40 µm thick transverse sections; n = 5 animals) counting in total 2438 neurons within the pre-BötC (figure 1e). The analysis revealed that a large percentage (62.4%) of pre-BötC neurons are glycinergic, as they show GlyT2 immunoreactivity. The majority of these glycinergic neurons also expressed 5-HT1AR (79.9%), while 73.9 per cent of neurons expressed 5-HT7R. Only 18.7 per cent of cells solely expressed 5-HT1AR, and 12.7 per cent expressed only 5-HT7R. A minority (7.4%) of cells lacked either 5-HT receptor. The remaining 37.6 per cent of pre-BötC cells may comprise glutamatergic or GABAergic neurons. The expression of 5-HT1AR and 5-HT7R was also high in the GlyT2-negative group of cells, 84.6 per cent of cells expressing 5-HT1ARs and 74.8 per cent of cells expressing 5-HT7Rs. A large portion of GlyT2-positive neurons also co-expressed μ-OR (figure 1f). The inference is that glycinergic neurons in the pre-BötC network do not only transmit, but evidently also receive signals via glycine receptors.
Following the observation that breathing is highly sensitive to opioids as well as a variety of 5-HTR signalling pathways, we tested for pharmacological effects of selective 5-HT1AR and 5-HT7R activation. The rationale was that agonists such as 8-OH-DPAT might also activate Gs-coupled 5-HT7Rs (Hedlund et al. 2004) and coactivation of these 5-HT7Rs might prevail over the inhibitory Gi/o–AC pathway to neutralize the 5-HT1AR-induced decline of intracellular cAMP levels (Hoyer et al. 2002).
We tested for 5-HT7R- and 5-HT1AR-specific effects in the perfused rat brainstem–spinal cord preparation, in which the respiratory network remains fully intact (Paton 1996). The network response was measured by recording phrenic nerve activity (PNA) and deriving minute respiratory activity (mPNA) online, before and after administering 5-HT1AR or 5-HT7R agonists or antagonists. Surprisingly, application of the 5-HT7R agonist 5-CT (0.3 µg ml−1) did not activate, rather it significantly reduced, mPNA (from 10.42 ± 3.98 to 4.47 ± 1.77; n = 5). mPNA activity was suppressed further (to 0.96 ± 0.41) when 5-HT1AR were additionally blocked with the selective antagonist WAY 100635 (figure 2a). Conversely, mPNA was augmented when the agonist 8-OH-DPAT was administered after 5-HT7Rs had been blocked with SB 269970 (figure 2b). Augmentation of mPNA activity occurred by enhanced burst frequency (9.54 ± 0.20 V s; p < 0.05) rather than an increase in PNA burst amplitude. Notably, the 5-HT1AR-dependent enhancement of PNA frequency by 8-OH-DPAT was abolished when glycine receptors were blocked by systemic application of strychnine. Strychnine application itself provoked a significant increase of mPNA (from 13.33 ± 1.03 to 22.73 ± 4.44; p < 0.05; n = 5), which did not change further (21.40 ± 4.64) when 5-HT1ARs were additionally activated by 8-OH-DPAT (figure 2c).
Respiratory network responses to systemic 5-HT1AR and 5-HT7R ligand applications of in situ rats. (a–c) Action of 5-HT1AR and 5-HT7R ligands in the perfused brainstem–spinal cord preparation. (a) Application of the 5-HT7R agonist 5-CT ...
Results were quite similar when relative changes of respiratory minute airflow (rRMA) were measured in vivo in anaesthetized rats. Administration of 8-OH-DPAT (n = 5) enhanced rRMA to 187 per cent (from 58.2 ± 8.4 to 109.3 ± 9.2; p < 0.01), an effect that was blocked by the 5-HT1AR-antagonist WAY 100635 (n = 3; figure 2d).
Taken together, these data demonstrate that the 8-OH-DPAT-induced increase of mPNA originates from a 5-HT1AR-specific modulation and is not simply explained by a 5-HT7R antagonism. The surprising network responses to 5-HT1AR and 5-HT7R stimulation, together with their disappearance after strychnine application, suggests that there is a signal-reversing process mediated through modulation of glycinergic inhibition.
To test for 5-HT1AR-specific modulation of inhibitory synaptic processes, single-electrode current- and voltage-clamp measurements of identified respiratory brainstem neurons were performed in pentobarbital-anaesthetized cats. In contrast to augmentation of synaptic inhibition by single-cell electrophoresis of 5-HT1AR agonists (Lalley et al. 1994; Richter et al. 1997), global activation of 5-HT1ARs by intravenous injection of 8-OH-DPAT provoked an increase in neuronal excitability owing to a diminution of synaptic inhibition as tested in expiratory (n = 12) and post-inspiratory (post-I; n = 4) neurons within brainstem coordinates that outline the localization of the pre-BötC in cat (Schwarzacher et al. 1995). The reduction in synaptic inhibition was most obvious in post-I neurons (figure 3), which are known to be glycinergic (Schmid et al. 1991; Ezure et al. 2003). These post-I neurons exhibited a release from early-inspiratory synaptic inhibition, causing them to advance their action potential firing far into the preceding inspiratory phase of the oscillatory cycle (figure 3a,c). At higher concentrations of 8-OH-DPAT, this release from early-inspiratory inhibition was combined with a membrane hyperpolarization, which limited action potential discharge to the late-inspiratory period, when post-I neurons are normally effectively silenced (figure 3a,b). In vivo voltage-clamp measurements performed at holding voltages of −75 to −90 mV, which is close to the chloride (inhibitory post-synaptic current) equilibrium potential (Haji et al. 2000), uncovered a clear inward current component, revealing that the process enabling the 8-OH-DPAT-provoked activity shift is linked to an excitatory synaptic input during inspiration. Under control conditions such an excitatory drive seemed to be shunted by synchronous synaptic inhibition (figure 3c–e).
Modulation of inhibitory synaptic interactions within the respiratory network of anaesthetized in vivo cats. (a and b) Two examples for the response of post-inspiratory (post-I) neurons with a mean membrane potential of −65 and −70 mV ...
The 5-HT1AR-induced change in the inspiratory phase-terminating discharge of inhibitory post-I neurons (Richter et al. 1987) is consistent with the corresponding network response to 8-OH-DPAT recorded from the phrenic nerve, consisting of shortened inspiratory burst duration and increased burst frequency (figure 3a). It is noteworthy that a similar effect has been described after strychnine blockade of glycinergic synaptic transmission (Busselberg et al. 2003).
The perception that the activation of rhythmic network output is accomplished by disinhibition of glycinergic neurons that could counteract opioid-induced depression of respiratory activity was confirmed with a sequential strategy of pharmacological treatment in the in situ perfused rat brainstem preparation. Opiate treatment was used to depress respiratory PNA, followed by the systemic administration of 8-OH-DPAT or buspirone, which is a clinically approved partial 5-HT1AR agonist (figure 4). After fentanyl had significantly reduced or arrested mPNA (from 6.76 ± 1.41 to 2.11 ± 0.58; p < 0.01), systemic administration of 8-OH-DPAT recovered mPNA to approximately 70 per cent of normal activity levels (4.95 ± 0.87 compared with 6.76 ± 1.41; p < 0.05; n = 6; figure 4a). The fentanyl-induced depression of mPNA (1.93 ± 0.73 versus 6.17 ± 1.55 V s; p < 0.01) was effectively re-activated with successive administration of buspirone (3.88 ± 0.88; p < 0.05; n = 4; figure 4b).
Treatment of opioid-induced depression of respiratory activity with 5-HT1AR agonists in rats. Treatment of perfused brainstem–spinal cord preparation with 8-OH-DPAT (a) and the clinically approved 5-HT1AR agonist buspirone (Buspar; b). Both drugs ...
The recovery from opioid-depressed respiratory activity as seen in wild-type rodents was also verified in vivo. The µ-OR-agonist fentanyl, which is sufficient to completely block the nociceptive TF reflex (TFR; defined as a threefold prolongation of the 7.35 ± 1.11 s TFR at control), induced apnoea in rats (figure 4c). Subsequent administration of 8-OH-DPAT restored breathing within 2–3 min (figure 4c). The relative rRMA reappeared with 0.21 ± 0.4 (p < 0.001) and increased steadily within the following 3 min (0.60 ± 0.03 compared with control values of 0.68 ± 0.06; p < 0.001), while the TFR was not restored. This effect was μ-OR specific, because the antagonist naloxone induced an increase in rRMA above control levels (1.03 ± 0.12 versus 0.68 ± 0.06) and also fully restored the TFR (figure 4c).
Real-time PCR analysis from total cervical spinal cord tissue of the segments C2–C4 revealed relative transcript levels of 1.09 ± 0.16 for 5-HT1AR, 1.04 ± 0.29 for 5-HT7R and 0.88 ± 0.12 for μ-OR. Immunohistochemical analysis of protein expression (five sections from five animals each) showed that 95.1 per cent of the μ-OR-immunoreactive dorsal horn neurons are also positive for the 5-HT1AR, whilst 5-HT7R was detected only in 53.4 per cent of cells (figure 5). Such strong colocalization of μ-ORs and 5-HT1ARs implies that synergistic signalling effectively reduces cellular cAMP that may account for a failure of nociceptive signalling in dorsal horn neurons.
5-HT1A, 5-HT7, and μ-OR expression in dorsal horn neurons of the rat spinal cord. Triple-labelling of 5-HT1A, 5-HT7 and μ-ORs in 40 µm thick sections from the cervical spinal cord (spinal segment C3/C4; overview in a) revealed ...
Global administration of cAMP-depressing modulators into a neuronal network that operates by synaptic interactions between excitatory and inhibitory neurons can be expected to induce depression of neuronal excitability that also provokes particular forms of disinhibition. These synaptic interaction-dependent effects are evident in the respiratory network, which operates with a reciprocal organization of antagonistic groups of glutamatergic, GABAergic and glycinergic interneurons (Richter 1996). The outcome of synaptic disinhibition depends on the specific action of these inhibitory interneurons (figure 6) whose contribution is revealed by the glycine and GABAA receptor antagonists—strychnine and bicuculline (Schmid et al. 1996; Busselberg et al. 2001). Modulation of glycinergic inhibition seems particularly effective, because it not only controls patterning of the steadily augmenting inspiratory (aug-I) and expiratory (aug-E) burst discharges, but also regulates the efficiency of respiratory phase control by early-inspiratory (early-I) and post-inspiratory (post-I) neurons (Richter 1982; Busselberg et al. 2001; Ezure et al. 2003; figure 6a). Glycinergic control of respiratory phase termination is vital (Pierrefiche et al. 1998) and, therefore, it is not surprising that more than 60 per cent of neurons within the pre-BötC are glycinergic, as identified by the neuronal glycine transporter GlyT2 (Gomeza et al. 2003; Tanaka et al. 2003). Some of these GlyT2-labelled inhibitory neurons were identified as respiratory in whole cell recordings (Winter et al. 2009). The GlyT2-positive inhibitory neurons also express 5-HT1AR and 5-HT7R (figure 1) and, therefore, are subjected to ongoing modulatory control by serotonin that is continuously released from raphé neurons (Connelly et al. 1989; Mason et al. 2007). Another important finding is that inhibitory glycinergic neurons themselves receive glycinergic inhibitory inputs (figure 1). This makes disinhibition a critical process in network adjustment.
Proposal of the network reorganization following global stimulation of 5-HT1AR. Based on the experimental data obtained in mouse, rat and cat, we propose a functional reorganization of network operation. (a) Starting from control, we conclude that the ...
A predominant modulatory effect of 5-HT is exerted through 5-HT1AR that is expressed in 80 per cent of all pre-BötC cells. A commonly used drug to test for the functional consequences of 5-HT1AR modulation is the agonist 8-OH-DPAT. When respiratory neurons are tested individually with locally restricted ionophoretic application of 8-OH-DPAT or with drugs that inhibit cAMP-dependent protein kinase A (PKA), individual neurons respond equally well with a significant enhancement of inhibitory synaptic currents (Lalley et al. 1997). This is particularly clear for the glycinergic processes mediated by post-I neurons (Richter et al. 1987, 1992) and such pronounced enhancement of glycinergic synaptic currents (Lalley et al. 1997; Richter et al. 1997) must be expected to alter the operation of the whole respiratory network. We propose a reorganization of network operation as is schematically shown in figure 6b. Discharge of early-I neurons is evidently abolished, because their inhibitory feedback to post-I neurons disappears (figure 3a). Disinhibition of post-I neurons causes a fundamental change in network operation, because it induces a notable shift of post-I neuronal firing into the preceding inspiratory phase (figure 6b, see also figure 3). Their time-shifted and prolonged discharge is supposed to be very efficient, because 5-HT1AR-induced modulation potentiates inhibitory synaptic currents in the innervated neurons (Lalley et al. 1997). These effects are only partially reduced but not abolished at higher 8-OH-DPAT concentrations when post-I neurons undergo a gK+-controlled membrane hyperpolarization (Lalley et al. 1994; Ballanyi et al. 1997) limiting their discharge to late-inspiratory periods.
The functional uncoupling of early-I neurons from the network, combined with phase-shifting of post-I firing into the inspiratory phase, leads to the disappearance of normal post-inspiratory activity and provokes a two-phased oscillation between inspiration and expiration. This is consistent with the observation that 5-HT1AR-evoked modulation is greatly reduced when glycine receptors were blocked by strychnine (figure 1d), while an identical ‘phase-shifting’ occurs in the discharge of post-I neurons (Busselberg et al. 2001; Dutschmann & Paton 2002). Post-I neurons originating from the pons (Dick et al. 1994; Dutschmann & Herbert 2006) should be modulated synergistically. A minor change in network excitability might occur by modulation of tonically active glycinergic inputs (Haji et al. 1992). In conclusion, the data suggest that glycinergic synapses are the critical target of 5-HT1AR-regulated modulation of network functions and that reinforcement of inhibitory glycinergic processes (Lalley et al. 1997) might protect against arrhythmic breathing disturbances.
μ-ORs are abundantly expressed in pre-BötC neurons (Manzke et al. 2003) including NK-1R-positive excitatory neurons (Gray et al. 1999) and GlyT2-positive inhibitory respiratory neurons (figure 1). When activated, μ-ORs depress neuronal excitability of all these cell types (Ballanyi et al. 1997; Mellen et al. 2003). At opioid concentrations sufficient to slow respiration, respiratory periods are prolonged, indicating that the onset of post-I neuronal discharge is delayed and burst duration is lengthened (Lalley 2006). Such suppressed post-I neuronal discharge seems to be insufficient for adequate termination of the inspiratory phase. Our finding of a 5-HT1AR-induced disinhibition of post-I neurons (figure 3), enabling them to shift their discharge into the preceding inspiratory phase and potentiation of their inhibitory postsynaptic currents, appears to significantly shorten the latency of inspiratory phase termination and thus the rescue of respiratory rhythmicity. The present experiments demonstrate that this effect is fairly robust and compensates for μ-OR-induced depression of neuronal bursting (Brunton & Charpak 1997; Wagner et al. 2000; Chieng et al. 2006). Further analyses are necessary to understand how the accompanying membrane hyperpolarization affects membrane properties necessary for endogenous and/or rebound bursting (Ramirez & Richter 1996; Ramirez et al. 1997; Butera et al. 1999; Del Negro et al. 2002).
In the sensory network of spinal nociceptive dorsal horn neurons, our immunohistochemical analysis showed that 95.1 per cent of the μ-OR-immunoreactive neurons coexpress 5-HT1ARs, while only 53.4 per cent of neurons express 5-HT7Rs (see figure 5). Therefore, the effects of 5-HT7Rs must be weaker, because the synergistic actions of μ-ORs and 5-HT1ARs should effectively depress cellular cAMP levels in this population of neurons. This should diminish PKA-dependent phosphorylation of the glycine receptors (Harvey et al. 2004) and potentiate synaptic inhibition to further enhance anti-nociception (Sandkuhler et al. 1987; Helmchen et al. 1995).
The present analyses of molecular, cellular and systemic processes reveal that in the case of reciprocally wired networks, predominant modulation of inhibitory neurons can prevail over depression of excitatory neurons to initiate a functional reorganization of the operational network machinery (figure 6c). The significance of such self-reorganization is evident in the respiratory network and explains how fentanyl depression of respiratory rhythmicity can be overcome with 5-HT1AR agonists (Sahibzada et al. 2000; Meyer et al. 2006). An important inference can be drawn: 5-HT1AR agonists may effectively protect against opiate-induced apnoea or severe slowing of breathing, but the underlying processes also induce a depression of inspiratory peak activity that affects tidal volume. Therefore, there is the possibility that the treatment could result in shallow breathing, which would require additional medical treatment.
The animal experiments were performed in accordance with the European Community and National Institutes of Health guidelines for the care and use of laboratory animals and were approved by the Ethics Committee of the Georg-August-University, Göttingen, Germany.
This work was supported by the DFG Research Center Molecular Physiology of the Brain (CMPB; FZT 103) and by the German Ministry for Education and Science (BMBF) via the Bernstein Center for Computational Neuroscience (BCCN) Göttingen under grant no. 01GQ0432. We are grateful to Anna-Maria Bischoff for excellent technical support during the course of the experiments.
One contribution of 17 to a Discussion Meeting Issue ‘Brainstem neural networks vital for life’.