Invariant TCR-specific mAb specifically react with human iNKT cell Vα24Jα18
Validation of invariant Vα24Jα18 CDR3 loop-specific staining. A. Histograms of Vα24, Vβ11, and 6B11 expression as determined for TCR-negative Jurkat cell subline transfected to transiently express iNKT Vα24Jα18 and Vβ11 TCR, as previously described for other CD1-reactive T cells . B. Liver mononuclear cells from human invariant Vα24Jα18 transgenic mice  or C57 wild type mice were specifically stained with 6B11-biotin or Vα24-biotin and fluorescent streptavidin and otherwise with mouse-specific directly-conjugated mAbs as shown. Note 6B11 identified high and low level expressing transgenic iNKT, whereas Vα24 was uniformly positive. This may reflect 6B11 having different affinity for Vα24Jα18 with distinct murine Vβ pairings. C–H. Splenic sections from human Vα24Jα18 transgenic (46) or C57 wild type mice were double-stained with 6B11 and mouse CD3 or isotype controls. Only the human iNKT transgenic spleens contained 6B11-reactive T cells.