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J Virol. 2010 May; 84(9): 4845–4850.
Published online 2010 February 17. doi:  10.1128/JVI.02514-09
PMCID: PMC2863727

Dengue Virus Inhibits the Production of Type I Interferon in Primary Human Dendritic Cells[down-pointing small open triangle]

Abstract

Dengue virus (DENV) infects human immune cells in vitro and likely infects dendritic cells (DCs) in vivo. DENV-2 productive infection induces activation and release of high levels of chemokines and proinflammatory cytokines in monocyte-derived DCs (moDCs), with the notable exception of alpha/beta interferon (IFN-α/β). Interestingly, DENV-2-infected moDCs fail to prime T cells, most likely due to the lack of IFN-α/β released by moDCs, since this effect was reversed by addition of exogenous IFN-β. Together, our data show that inhibition of IFN-α/β production by DENV in primary human moDCs is a novel mechanism of immune evasion.

Dengue virus (DENV) belongs to the family Flaviviridae and has great importance in the areas of medicine and biodefense (3, 17). Transmitted by Aedes aegypti, DENV is the most prevalent arthropod-borne human virus (34). Generally, infected patients experience dengue fever, but 2 to 20% of cases manifest dengue hemorrhagic fever (DHF), a severe and often lethal illness (34). Dendritic cells (DCs) initiate immune responses and become activated upon contact with pathogens (2), with upregulation of costimulatory molecules and release of proinflammatory cytokines (2). Secretion of type I interferon (alpha/beta interferon [IFN-α/β]) by DCs contributes to the generation of antiviral innate and adaptive immune responses (7, 16, 18, 32). DCs, together with other cell populations, have been suggested as target cells for DENV infection (12, 14, 15, 35).

We investigated the replication of DENV-2 in monocyte-derived DCs (moDCs) obtained by culturing CD14+ cells, isolated from blood of healthy donors, for 6 days in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin 4 (IL-4), and human serum (9). The purity obtained by flow cytometry was routinely 96 to 99% CD14 CD11c+ HLA-DRlow moDCs (7). DENV-2 (16681 and NGC strains) and DENV-3 (Hawaii strain) were grown in C6/36 insect cells (5) and were titrated by plaque assay (4). moDCs were infected with DENV-2 at different multiplicities of infection (MOIs), and viral replication was tested by quantitative reverse transcription-PCR (qRT-PCR) (9, 28). All infections in this study were performed in moDCs from at least three independent donors with three replicates per sample. DENV-2 replication peaked at 48 h after infection (Fig. (Fig.1A),1A), and release of newly synthesized infectious particles had a similar kinetics (Fig. (Fig.1B),1B), indicating productive infection of moDCs. As a measure of moDC activation, proinflammatory cytokine levels (IL-6 and tumor necrosis factor alpha [TNF-α]) were quantified by enzyme-linked immunosorbent assay (ELISA) 24 h after DENV-2 infection (Fig. (Fig.1C).1C). We observed high expression of those cytokines, comparable to cytokine expression after poly(I:C) treatment of moDCs. Interestingly, UV-inactivated DENV-2 did not induce the moDC activation, suggesting that DENV-2 replication is required to activate moDCs. Infection with influenza virus A/PR8/33 (PR8) showed low levels of cytokine induction due to the activity of the NS1 protein (6). Flow cytometry analysis showed high upregulation of CD86 and HLA-DR markers on DENV-2-infected moDCs, comparable to that observed with Newcastle disease virus (NDV) infection (Fig. (Fig.1D).1D). NDV (Hitchner strain) and PR8 viruses, used as controls, were grown in 9-day-old embryonated chicken eggs (8, 24, 27). Together our data demonstrate that DENV-2 is able to productively infect and strongly activate human moDCs.

FIG. 1.
Infection and activation of moDCs with DENV-2. (A) DENV-2 RNA levels were measured by specific qRT-PCR of the NS5 in moDCs infected with the indicated MOIs of DENV-2 at different times after infection (6, 24, 36, and 48 h). (B) Titration by plaque assay ...

Type I IFN plays an important role in DENV infection, as low levels of the IFN-α/β producing plasmacytoid DCs (pDCs) have been observed in DHF patients (29). Also, the ability of DENV to inhibit IFN-α/β signaling in cells has been recognized (21) and attributed to several DENV proteins (1, 13, 20, 22, 23). In human moDCs, DENV-2 antagonizes IFN-α/β but not IFN-γ signaling (10). Because of the importance of IFN-α/β in the generation of antiviral immune responses (7, 16, 18, 32), we measured functional IFN-α/β by performing a bioassay in Vero cells using green fluorescent protein-tagged NDV (NDV-GFP) (27). Supernatants from mock- and DENV-2-infected moDCs did not impair NDV-GFP infection (Fig. (Fig.2A).2A). However, supernatants from NDV-infected moDCs considerably reduced the number of GFP-positive cells, achieving about a 50% reduction at the highest dilution. When we used different strains or serotypes of DENV, New Guinea C (DENV-2 NGC) and DENV-3 (Hawaii strain) (Fig. (Fig.2B),2B), neither one was able to induce significant IFN-α/β production. These results strongly indicate that moDCs do not produce functional IFN-α/β after DENV infection. We used qRT-PCR to extensively analyze the expression profile of genes of the IFN-α/β pathway as well as moDC activation genes. We observed a marked chemokine profile (IL-8, MIP1β) as early as 2 h after DENV-2 infection (Fig. (Fig.2C),2C), as has also been observed in patients' sera (30), suggesting a possible mechanism of DENV-2 to attract cells for virus amplification. This is in contrast with the cytokine/chemokine profile observed in NDV-infected moDCs (Fig. 2C and D), where no IL-8 or MIP1β production was observed at early times after infection (Fig. (Fig.2C)2C) and high levels of TNF-α were induced. According to the bioassay data, there was no expression of IFN-α/β after DENV-2 infection as opposed to NDV infection. However, some IFN-inducible genes, like Mx1, were upregulated by DENV-2 18 h after infection (Fig. (Fig.2D).2D). This finding has been previously observed in Rhesus macaques after DENV infection (31) and has also been detected in human moDCs after infection with influenza viruses that have the intact IFN antagonist NS1 protein (9). It is likely that traces of IFN-α/β under our level of detection produced by infected and/or surrounding cells may induce the expression of those interferon-stimulated genes (ISGs). Nevertheless, the induction levels of Mx1 were significantly lower after DENV than after NDV infection (Fig. (Fig.2D2D).

FIG. 2.
Type I IFN bioassay and gene expression profile of moDCs after DENV-2 infection. Vero cells were infected with NDV-GFP after incubation for 24 h with serial dilutions of supernatants collected from moDCs that had been infected with DENV-2 or NDV or mock ...

Additionally, we measured the IFN-α protein levels in the supernatant of moDCs and pDCs after DENV-2 or NDV infection (Fig. (Fig.3)3) using a panspecific human IFN-α ELISA. We observed extremely low IFN-α levels in supernatants of DENV-2-infected moDCs and only later than 36 h after infection (Fig. (Fig.3A).3A). UV-inactivated DENV-2 did not induce IFN-α production (data not shown), indicating that binding to the receptor is not sufficient to induce an antiviral response. However, NDV-infected moDCs showed a fast and high IFN-α production (Fig. (Fig.3B).3B). pDCs, in spite of DENV replication (data not shown), did not show any significant IFN-α production 8 h after infection, in contrast with the strong induction after NDV infection (Fig. (Fig.3C).3C). Our results clearly demonstrate that DENV-2 is able to block or may not induce IFN-α/β in human primary immune cells. Although some reports showed low levels of IFN-α/β production after DENV infection (11, 25, 26, 33), the measurements were performed 24 h or later after infection and were performed using a high MOI, which does not reflect IFN induction by viruses and may overestimate the ability of DENV to induce this cytokine. To identify a possible mechanism of the absence of IFN-α/β induction, we analyzed the phosphorylation levels of the IFN regulatory factor 3 (IRF-3), an important element in the IFN-α/β induction pathway. We could not detect IRF-3 phosphorylation in DENV-2 infected moDCs (Fig. (Fig.3D)3D) by Western blotting, although 58% of moDCs were infected by DENV-2 at an MOI of 1. In contrast, NDV infection induced strong IRF-3 phosphorylation in moDCs 12 h after infection, with 63% of the moDCs being infected with this virus. This observation indicates that DENV-2 inhibits IFN-α/β production in moDCs upstream or at the level of IRF-3 phosphorylation, while the NF-κB pathway seems unaffected, since we observed high levels of proinflammatory cytokines and chemokines after DENV infection (Fig. (Fig.1C1C and and2C).2C). Taken together, these results indicate that DENV-2 is able to induce a strong activation of the moDCs, with the notable exception of IFN-α/β production.

FIG. 3.
Impaired IFN-α/β interferon production by moDCs after DENV-2 infection. IFN-α levels in cell supernatants were quantified by a panspecific IFN-α ELISA kit at the indicated time points after infection of the moDCs with DENV-2 ...

Since production of IFN-α/β is crucial for T-cell priming in virus infections (16, 32), and since exposure of DCs to IFN-α/β prior to infection with influenza virus primes those cells to better responses (28), we analyzed the ability of DENV-2-infected moDCs to prime T cells. We incubated moDCs that were either noninfected, treated with lipopolysaccharide (LPS) (0.5 μg/ml), or infected with NDV (MOI of 0.5) or DENV-2 (MOI of 1) with allogeneic naïve T cells at a 1:1 ratio (105 DCs/105 T cells). T cells isolated from buffy coats were labeled with carboxyfluorescein succinimidyl ester (CFSE; 5 μM) in order to measure their proliferation by flow cytometry. All moDCs, independently of the treatment, were able to induce T-cell proliferation (Fig. (Fig.4A),4A), indicating viability and lack of cytotoxicity of DENV-2-infected moDCs. LPS induced a stronger activation of moDCs, resulting in higher T-cell proliferation in the cocultures. Addition of recombinant human IFN-β to the coculture medium had a minimal effect on T-cell proliferation (Fig. (Fig.4A).4A). No proliferation was observed in T cells alone, in either the presence or absence of exogenous IFN-β (data not shown). T cells cocultured with DENV-2-infected moDCs produced very small amounts of IFN-γ at day 2 that did not increase with time (Fig. (Fig.4B).4B). This observation agrees with the impaired T-cell proliferation seen in patients with acute DENV-2 infection (19). However, T cells cocultured with either NDV-infected or LPS-treated moDCs produced larger amounts of IFN-γ that increased with time. To demonstrate that the lack of IFN-α/β production by DENV-2 infected moDCs was responsible for their impaired ability to prime T cells, we performed cocultures in the presence of recombinant human IFN-β (1,000 and 2,000 U/ml). The results shown in Fig. Fig.4C4C clearly demonstrate that addition of exogenous IFN-β significantly restored the production of IFN-γ by T cells, indicating that production of IFN-α/β by moDCs is crucial for the priming of T cells. On the other hand, addition of IFN-β to the cocultures of T cells with NDV-infected moDCs did not increase the levels of IFN-γ (Fig. (Fig.4D),4D), since the IFN-α/β produced by NDV-infected moDCs might be sufficient to induce T-cell priming. Addition of IFN-β to the cocultures with mock-infected moDCs as well as to the T cells alone did not have any effect on the release of IFN-γ (data not shown). Altogether, these data indicate that infection of moDCs with DENV-2 impairs their ability to prime T cells regardless of the strong activation induced in those cells, due to the lack of IFN-α/β produced in those cells after infection.

FIG. 4.
DENV-2-infected moDCs fail to induce Th1 responses in allogeneic T cells. Allogeneic T cells were cocultured for 2, 3, and 4 days with DENV-2 (MOI = 1)- or NDV (MOI = 0.5)-infected moDCs (ratio, 1:1). As positive and negative controls, ...

In conclusion, our results provide new data about how DENV-2 can induce activation of moDCs and release of proinflammatory cytokines, except IFN-α/β. We also demonstrate that DENV-2 infection blocks IRF-3 phosphorylation in moDCs, impairing the subsequent IFN-α/β production in response to the infection. This lack of IFN-α/β production renders DENV-infected moDCs incapable of priming T cells toward antiviral Th1 responses, regardless of other markers of activation observed in those moDCs after DENV-2 infection. These data strongly suggest that lack of IFN-α/β induction in immune cells after DENV infection contributes to the inefficient and cross-reactive adaptive immune responses observed in patients. Since therapeutic options for dengue remain modest due to the lack of vaccine or antiviral treatments available, our findings will help in the design of treatments for dengue disease, as they suggest that targeting IFN-α/β production may be an efficient antiviral therapy for DENV.

Acknowledgments

We thank Jorge Munoz-Jordan, Sujan Shresta, Irene Bosch, and Maudry Laurent-Rolle for protocols and reagents. We also acknowledge Hannah Phipps-Yonas, for help with pDC isolations and Dailia Francis for contributions during early stages of the experiments.

This work was funded by 1R01AI073450-01A2 (A.F.-S.), U19 AI62623 (A.F.-S.), and a Ramon Areces Foundation fellowship (J.R.R.-M.).

We have no competing financial interests.

Footnotes

[down-pointing small open triangle]Published ahead of print on 17 February 2010.

The authors have paid a fee to allow immediate free access to this article.

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