PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

Logo of aacPermissionsJournals.ASM.orgJournalAAC ArticleJournal InfoAuthorsReviewers
 
From:
Published online 2010 February 16. doi: 10.1128/AAC.01384-09

FIG. 3.

An external file that holds a picture, illustration, etc.
Object name is zac9991089110003.jpg

(A) Flagellin level of wild-type P. mirabilis (N2), the ugd knockout mutant (dU2), and the galU knockout mutant (dG1). The flagellin level was determined at different time points after seeding the strains on LB agar plates. The value obtained with the wild-type cells at 4 h postseeding was set at 100%, and all other values were expressed relative to this value. The data represent the averages of results of three independent experiments with standard deviations. (B) Microscopic observation of cell differentiation of wild-type P. mirabilis (N2), the ugd knockout mutant (dU2), and the galU knockout mutant (dG1). Cells were Gram-stained and viewed under oil (magnification, ×1,000). Three independent experiments were performed, and the representative pictures showing cell differentiation at 4, 5, and 6 h postseeding on LB agar plates are shown. The increase in cell length was taken as a sign of cell differentiation. (C) Expression of flhDC mRNA in wild-type P. mirabilis (N2), the ugd knockout mutant (dU2), and the galU knockout mutant (dG1). Total RNA was isolated from respective cells at 4 h postseeding on LB agar plates and was then subjected to real-time RT-PCR for the measurement of flhDC mRNA. The value obtained with the wild-type cells was set at 1. The data represent the averages of results of three independent experiments with standard deviations.

Images in this article

  • FIG. 1.
  • FIG. 2.
  • FIG. 3.
  • FIG. 4.
  • FIG. 5.
  • FIG. 6.
Click on the image to see a larger version.