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J Bacteriol. 2010 May; 192(10): 2645–2646.
Published online 2010 March 26. doi:  10.1128/JB.00159-10
PMCID: PMC2863575

Complete Genome Sequence and Annotation of the Treponema pallidum subsp. pallidum Chicago Strain [down-pointing small open triangle]


In syphilis research, the Nichols strain of Treponema pallidum, isolated in 1912, has been the most widely studied. Recently, important differences among T. pallidum strains emerged; therefore, we sequenced and annotated the Chicago strain genome to facilitate and encourage the use of this strain in studying the pathogenesis of syphilis.

Syphilis continues to be a common and serious disease, affecting at least 25 million adults worldwide (4). It is a recognized cofactor in the transmission and acquisition of HIV (2, 6) and is a major cause of stillbirth and perinatal morbidity in the developing world (11, 12). The peculiar biology of the causative agent of syphilis, Treponema pallidum subsp. pallidum (T. pallidum), along with the inability to continuously grow this pathogen in vitro, has hindered progress in this field. However, syphilis research has greatly benefited from the completion of the T. pallidum Nichols strain genome sequence, published in 1998 (3). The Nichols strain of T. pallidum was isolated in 1912 from the cerebrospinal fluid (CSF) of a patient with secondary syphilis, has become the laboratory reference strain for the study of syphilis, and has consequently been passed in rabbits for nearly a century. Our growing understanding of the mechanisms behind T. pallidum's ability to evade the host immune response to persist in the host, however, would not be possible without access to strains that have not undergone continual animal passage. The Chicago strain (14), first isolated in 1951, has not been continually passed in rabbits and has been critical to our understanding of fundamental aspects of the pathogenesis of syphilis, such as antigenic variation, immune escape, and persistence of the pathogen in the host (1, 5, 7). We sequenced and annotated the Chicago genome to facilitate and encourage the use of this strain in addressing questions concerning the pathogenesis of syphilis.

Genomic DNA isolated from the T. pallidum Chicago strain, initially obtained from Paul Hardy and Ellen Nell, was prepared for Illumina-based sequencing using the Paired End DNA sample preparation kit (Illumina Inc., San Diego, CA) following the provided protocol. Genome sequencing was performed at the Center for Genome Research and Biocomputing (CGRB) at Oregon State University (Corvallis, OR) using a Genome Analyzer IIxSystem (Illumina Inc.). A first draft of the Chicago strain genome was assembled using the reference-guided assembly program Maq (8) with the T. pallidum Nichols strain genome (3) as reference. Regions in the reference-guided assembled genome where Maq could not resolve sequence were then compared to contiguous sequences assembled through the use of the de novo assembly software VCAKE, and a single contiguous draft sequence was then produced (13). Nucleotide changes between matched pairs were located using the Diffseq program from the Emboss software suite. For annotation, the Chicago strain genome sequence was submitted to the J. Craig Venter Institute (JCVI) Annotation Service (

The Chicago genome is 1,139,281 bases long and encodes 1,081 open reading frames (ORFs). Forty-four nucleotide substitutions, 21 deletions (involving 30 nucleotides [nt]), and 75 insertions (involving 1,303 nt) were found by Diffseq software compared to the Nichols strain genome.

The Chicago genome will provide further insight into the poorly understood subject of genetic variability among syphilis strains (3, 9). Genetic variability among T. pallidum strains is likely one of the factors influencing the protean manifestations of syphilis infection which are seen both clinically and experimentally (10), possibly reflecting differential virulence among strains or niche differences.

Nucleotide sequence accession number.

The complete genome sequence of Treponema pallidum subsp. pallidum Chicago was deposited in the GenBank database and assigned accession number CP001752.


We are thankful to Elizabeth Miko Robertson for helping with manuscript preparation and submission.

This work was supported by NIH grants AI42143 and AI63940 (to S.A.L. and A.C.-L.).


[down-pointing small open triangle]Published ahead of print on 26 March 2010.


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