Several etiologic classification systems have been devised for ischemic stroke. Some failed to revise their rules according to the needs of the time and therefore have expired [1
]. Others have attained limited acceptance and use by the community [4
]. The following section provides a background on the most modern and commonly applied classification systems.
The TOAST System
The TOAST system was developed for use in a therapeutic acute ischemic stroke trial in the early 1990s [3
]. The system is based primarily on clinical features but also uses existing diagnostic information from CT, MRI, transthoracic echocardiography, extracranial carotid ultrasonography, and, when available, cerebral angiography. The TOAST system is composed of five major subtypes (): large artery atherosclerosis, cardiac embolism, small artery occlusion, stroke of another determined cause, and stroke of an undetermined cause. There are two possible states (probable or high risk and possible or low risk) for the first four categories, whereas the last category is broken down further into cursory evaluation, unknown group, and patients with two or more potential causes. These categories give rise to an 11-subtype system. Details of the definitions of each category may be found in the original publication [3
Important characteristics of etiologic classification systems for ischemic stroke
Compared with earlier classification systems [1
], the TOAST system uses more objective criteria for subtype diagnoses. The diagnosis of large artery atherosclerosis requires vascular imaging evidence of an atherosclerotic lesion causing more than 50% stenosis. Cardiac causes are arbitrarily divided into high-risk and medium-risk sources based on their relative potential to cause stroke. The diagnosis of lacunar infarction is confirmed based on the size (>1.5 cm in diameter) and location (brainstem, subcortical white matter) of the ischemic lesion on brain imaging. Additionally, this classification system incorporates the completeness of diagnostic investigations into subtype assignments; a “probable” subtype is diagnosed only if diagnostic evaluation findings exclude other etiologies. A “possible” diagnosis is made if there is evidence of one subtype, but diagnostic studies for other subtypes are not done.
Although the TOAST system has been used as the gold standard classification system for almost two decades, it is becoming apparent that it has important limitations. The TOAST system assigns patients with more than one etiology to a distinct category (two or more causes or unclassified category). Although this approach is intended to enhance the accuracy of assignments to other etiologic categories, frequent detection of multiple competing etiologies with a present-day stroke workup results in categorization of approximately half of all stroke patients into the “unclassified” group [5
]. Likewise, because diagnostic investigations in routine clinical practice often are stopped when a positive test finding is obtained, etiologic subtypes frequently are assigned a low level of confidence (“possible”) in the TOAST system. Decisions regarding subtype assignments based on raters’ personal opinions rather than published TOAST rules result in a reduction in the size of “unclassified” and “possible” categories, but at the expense of reliability. Several studies have assessed the TOAST system’s reliability. Despite the initially reported high interrater reliability in the original publication [3
], subsequent studies from independent investigators have consistently demonstrated a moderate reliability for the TOAST system, with kappa values ranging from 0.42 to 0.54 [25
]. A computerized algorithm using the TOAST rules slightly improved the reliability (kappa=0.68), yet the 95% confidence intervals around the point estimate for kappa were large (0.44–0.91) and the reliability assessment was performed by only two raters [26
The CCS was devised to overcome the major limitations of the TOAST system. The primary goal was to achieve high reliability without inflating the “unclassified” category. The system attempts to accomplish its goal by developing a framework that is well defined, easily replicable, and fully evidence based [6••
]. It incorporates multiple aspects of present-day diagnostic stroke evaluation (diffusion-weighted imaging, perfusion-weighted imaging, CT and magnetic resonance angiography of extracranial and intracranial arteries, transthoracic and transesophageal echocardiography, and Holter monitoring) in a regulated manner to identify both causative and phenotypic subtypes.
The CCS categorizes ischemic stroke into major etiologic groups similar to those of the TOAST system (). However, the definitions for the subtypes are slightly different from those of TOAST: large artery atherosclerosis is defined as either occlusive or stenotic (ie, ≥ 50% diameter reduction or <50% diameter reduction with plaque ulceration or thrombosis, or plaque with ≤50% diameter reduction at the site of the origin of the penetrating artery supplying the region of an acute lacunar infarct) vascular disease judged to be the result of atherosclerosis in the clinically relevant extracranial or intracranial arteries. Cardiac sources of embolism are segregated into high- and low-risk categories with reference to an objective 2% primary stroke risk threshold. The diagnosis of small vessel occlusion is considered when there is imaging evidence of a single, clinically relevant acute infarction less than 20 mm in greatest diameter within the territory of basal or brainstem penetrating arteries in the absence of any focal pathology in the parent artery at the site of the origin of the penetrating artery (eg, focal atheroma, parent vessel dissection, vasculitis, vasospasm). As in the TOAST system, the “undetermined” category in the CCS is broken into subcategories: unknown, incomplete evaluation, unclassified stroke (more than one etiology), and cryptogenic embolism. The last subgroup, cryptogenic embolism, is a new category aiming to identify patients with angiographic evidence of an abrupt cutoff in an otherwise normal-looking artery or subsequent complete recanalization of a previously occluded artery. Segregation of such patients into a distinct category may give researchers the opportunity to study new emboli sources in a more refined way.
Major subtypes in the CCS are defined in three grades according to the weight of causal evidence: evident, probable, and possible. An etiology is deemed evident only if it is the sole potential mechanism that carries a high risk for stroke. A mechanism is classified as possible if there is no evident cause of stroke. A possible mechanism is associated with either low or uncertain risk for stroke. When there is more than one evident stroke etiology, a probable stroke mechanism is assigned based on the presence of clinical qualities that make a particular mechanism a more likely cause of stroke. The weight of causal evidence is determined according to quantitative primary stroke risk estimates associated with each cause. These risk estimates are used to rank multiple stroke mechanisms to identify the most probable cause. Additionally, specific clinical or imaging features that make one mechanism more probable than others (eg, internal watershed infarcts for large artery atherosclerosis, multiple acute infarcts in both anterior or both anterior and posterior circulations for cardio-aortic embolism, and stereotypic lacunar transient ischemic attacks within the preceding week for small vessel disease) also are used to determine the strength of causal relationships. The CCS identifies the certainty around causal associations by taking into account the comprehensiveness of the diagnostic evaluation. The influence of a missing diagnostic test on the final subtype classification is determined on a patient-by-patient basis depending on other diagnostic test findings and clinical stroke features. For example, the absence of echocardiography does not change subtype assignment in a patient with atrial fibrillation (cardio-aortic embolism), may lower the level of confidence in a patient with large artery atherosclerosis (possible large artery atherosclerosis), and prevents the assignment of large artery atherosclerosis as the cause in a patient with concurrent systemic embolism (incomplete evaluation). Finally, in the CCS, the weight of causal evidence for each etiology is graded according to the presence of a spatial relationship between the brain infarct and its cause (eg, multiple acute infarcts in both hemispheres, indicating a cardio-aortic source) and a temporal relationship between a specific event and the brain infarct (eg, stroke following cardiac or vascular surgery, acute myocardial infarction, and acute arterial dissection).
An automated system incorporating all the rules used in the CCS classifies the cause of stroke based on an analytic weighting of all the identified stroke etiologies and the strength of the evidence supporting them. It is a web-based tool available for free at http://ccs.mgh.harvard.edu
for academic use. The automated CCS intends to limit interexaminer variability in interpreting stroke-related characteristics and to ensure consistency in data entry, thereby maximizing interexaminer reliability in stroke classification. The interrater reliability of the CCS for causative subtyping has been assessed in three studies. An internal assessment by two raters revealed excellent reliability (kappa=0.90) for five major subtypes [5
]. A subsequent external assessment by five raters from four centers also demonstrated excellent reliability (kappa = 0.86) [6••
]. A third international multicenter study confirmed the earlier findings on reliability [30
]. The percentage of patients assigned to the unclassified group in these reliability studies ranged from 0% to 12%, suggesting that the automated system reliably classifies patients into known subtypes without expanding the unclassified category.
The ASCO Classification of Ischemic Stroke
ASCO is a typical phenotypic system that categorizes stroke patients according to a combination of their etiologic characteristics [7•
]. Its definitions for subtypes are based largely on expert opinion and are slightly different from those in other systems. A definite diagnosis of atherothrombosis is considered when there is atherosclerosis in the clinically relevant artery causing more than 70% stenosis or less than 70% stenosis with attached luminal thrombosis, or mobile thrombus in the aortic arch. A definite diagnosis of small vessel disease requires the demonstration of a deep infarct with a diameter less than 15 mm plus the presence of old lacunar infarct or leukoaraiosis or recent, repeated, similar transient ischemic attacks. Cardiac sources of embolism are stratified arbitrarily into various risk groups based on their relative potential to cause stroke.
ASCO incorporates the quality of diagnostic evaluation in its subtype assignments in different grades: 0 for conditions in which diagnostic evaluation reveals no abnormality, 1 for the presence of a definite cause based on direct demonstration by a gold standard test, 2 for the presence of an uncertain cause based on evidence from tests with imperfect sensitivity and specificity, 3 for circumstances in which disease is present but not likely a direct cause of stroke, and 9 for the inability to perform relevant diagnostic tests for a given subtype. Overall, the system classifies ischemic stroke into 625 phenotypic subtypes. According to the ASCO system, a patient with large artery atherosclerosis, atrial fibrillation, and lacunar infarction is classified as having “large artery atherosclerosis + small vessel disease + cardiac embolism,” or A1-S1-C1-O0.
The ASCO system takes into account certain stroke features that are not necessarily causally related to the event of stroke, such as leukoaraiosis, chronic microbleeds, Virchow-Robin spaces, and clinically irrelevant atherosclerotic disease. Accounting for factors that correlate with baseline burden of vascular disease may facilitate patient selection for large studies in which phenotype is more important than the underlying cause. For instance, in a study exploring genetic determinants of small vessel disease, it may be justified to study patients with leukoaraiosis even when the index stroke is caused by nonlacunar mechanisms. Because some of the concurrent conditions are quite prevalent in a typical stroke cohort and they usually are subject to significant interrater disagreement, the overall impact of including concurrent features on the system’s validity and reliability remains to be seen. According to the published ASCO rules, a patient with a cryptogenic M1 embolism with large hemispheric infarction and leukoaraiosis is classified as having “small vessel disease present” (A0-S3-C0-O0), which, once again, highlights the importance of interpreting phenotypic and causative subtypes in a completely different context.