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Acta Myol. 2008 October; 27(2): 54–58.
PMCID: PMC2858935

Phenotypic variability in siblings with Calpainopathy (LGMD2A)

Summary

Calpainopathy is an autosomal-recessive limb girdle muscular dystrophy (LGMD2A) characterized by selective atrophy and weakness of proximal limb girdle muscles. The clinical phenotype of the disease is highly variable inter-familial, but little is known about intra-familial variability. This study reports the phenotypic variability in eight sibling pairs with genetically proven LGMD2A. Although siblings with identical mutations were often similarly affected, in some families the age of onset and the clinical course varied considerably.

Keywords: Calpainopathy, limb-girdle muscular dystrophy, genotype phenotype correlation, LGMD2A, sibling

Introduction

Limb-girdle muscular dystrophies (LGMDs) are a clinically and genetically heterogeneous group of disorders characterized by progressive proximal weakness not caused by a primary dystrophin deficiency. Calpainopathy (LGMD2A) is the most common form of recessive LGMD (1). LGMD2A is caused by mutations in the CAPN3 gene (15q15.1-15.3), encoding calpain 3, a calcium dependent protease (2). So far, 445 unique allelic variants are known to be responsible for LGMD2A (Leiden database: www.dmd.nl). The phenotypic features involve atrophy of the pelvic, scapular and trunk muscles, but not of cardiac or facial muscles. Mental status is normal. Age at onset is between 8 and 15 years for at least two-thirds of the patients, with a range of about 2 to 40 years. The slowly progressive course of the disease leads to loss of ambulation during adulthood and a near-normal life expectancy. Serum creatine kinase (CK) is markedly elevated and a muscular biopsy shows a dystrophic picture with evidence of necrosis and regeneration. Due to the variability of the phenotype the final diagnosis of LGMD2A relies on protein analysis of the muscle biopsy (Western blot) and mutation detection in the CAPN3 gene. Although there is marked phenotypic variability between different families with LGMD2A, so far little is known about the intra-familial variability. Here we report on 8 siblings and their phenotypic variation which is particularly important for counselling of family members of patients with LGMD2A.

Materials and methods

Families were included when two siblings showed a LGMD phenotype with a more than ten-fold elevation of serum creatine kinase, and the findings of muscle biopsy and mutation analysis of CAPN3 confirmed the diagnosis of LGMD2A in at least one sibling. Information on age at onset and course of the disease were collected by chart review and each patient was examined clinically by one of the authors.

Muscle biopsies were analyzed by routine histology in 9/16 patients and Western blot analysis using a Calpain-3 antibody (Novocastra) was performed in 6/16 patients. Mutation analysis was performed by direct sequencing of the 24 exons and flanking intronic sequences of CAPN3 after PCR from genomic DNA, as previously described (3).

Case study

We identified 8 families with 2 affected siblings (Table (Table1).1). Of the 16 patients, 10 were female and 6 male with an age range from 9.5 to 36.8 years (mean 21.8, SD 9.6). The mean age at onset of LGMD2A, in these patients, was 9.3 years (range 4-17 years). Within siblings, the difference of age at disease onset was between 1 and 11.5 years (mean 3.1 years). First symptoms were toe walking, weakness in the lower limbs, proximal weakness, and scapular winging. In 4 patients, the first recognized distinctive feature was an increased CK between 1300 and 8688 U/L (mean 4290 U/L). CK level at onset was markedly increased in all patients tested (mean 4046 U/L) and there was no difference between the sexes. Current age of the patients is between 9.5 and 36.8 years and 4 patients are no longer able to walk independently (patients 2A, 2B, 7B, 8A). Seven sib pairs have the same or similar clinical course and symptoms. However, the older sibling is usually more affected. In one sib pair (6A and 6B), the younger sibling has a proven mutation, but, at age 9, no clinical symptoms. There is no clear correlation between age of onset and clinical course. Although increased serum creatine kinase was the only manifestation at the time of diagnosis, all patients developed clinical symptoms during the course of the disease. A muscle biopsy was performed in 9 patients and showed a dystrophic picture with increase of connective tissue in all patients. Frozen muscle tissue for immunoblot analysis of Calpain-3 expression was available in 6 patients. In 5 patients, there was no detectable expression of Calpain-3 and in one it was markedly reduced. We have identified 8 different mutations, all of which previously described (Table (Table1).1). In 3 families, the patients carried homozygous mutations whereas 4 sib-pairs were compound heterozygotes and in one family only one mutation could be detected. The most frequent mutation was c.550delA in exon 4, present in 5 families; one Russian family (family 8) was homozygous for this mutation.

Table 1
Eight families with siblings with calpainopathy.

Discussion

We present here a retrospective analysis of a series of siblings with a genetically confirmed diagnosis of LGMD2A (calpainopathy). Although intra-familial variability has been described in other LGMD subtypes in more detail, there are only a few reports on siblings with LGMD2A. Saenz et al. published a series of 238 LGMD2A patients belonging to 187 different families (1). For many patients, details of the clinical course were not available but for one sib-pair a difference in the age of onset of two years was mentioned. Fardeau et al. reported 12 families from a remote area of the Réunion Island with a high degree of consanguinity (4). There were 5 sib pairs and one group of 4 siblings included. Age at onset differed up to 4 years in 4 of the sib pairs and was at the same age in one sib pair and in 3 out of the 4 siblings and delayed by 2 years in the fourth sibling. Age at loss of ambulation was recorded for at least two of the siblings in four families and differed by 5 to 12 years (4). Also Guglieri et al. reported 77 patients with LGMD2A, including 6 siblings, but without more detailed intra-familial clinical details (5). Another 23 patients with LGMD2A, from 14 families, have been described by van der Kooi et al., showing intra-familial clinical phenotypes in siblings (6). The age at onset in that study differs mostly within the families. In two families, the onset of the disease was at the same age in siblings. In our study, age of onset differed by more than two years between siblings in 4 out of 8 families, confirming data shown by van der Kooi et al. In some families, this might be due to the fact that symptoms were noted earlier in the younger child after the diagnosis had been made in the older. However, in family 1, the diagnosis of LGMD2A was first made in the younger brother at the age of 5 years, thus the range of difference in age of onset is 11 years. At that time, the sister (three years older) did not show any symptoms of muscle weakness and, in fact, she did not develop evidence of muscle weakness until the age of 16 and, at age 20, she has only minor weakness of hip girdle muscles. Serum creatine kinase was markedly elevated in all patients and muscle biopsies, where available, invariably showed a dystrophic picture. Although the sensitivity of immunoblot analysis seems to be lower than previously assumed, in our series it was abnormal in all samples tested. Mutations in the CAPN3 gene could be identified in all families. The most frequent mutation was c.550delA in exon 4. This mutation is considered to be the most frequent one in Europe probably due to a founder mutation originating in the Eastern Mediterranean area (79). Our data suggest that it is also widely represented in German patients with LGMD2A as also observed by Hanisch et al., at the same time (10).

In conclusion, even in siblings with identical mutations, the age at onset and the clinical course of LGMD2A can vary considerably suggesting other genetic or environmental factors influencing the disease course. This is relevant for counselling family members of patients with LGMD2A and also leads to the conclusion that LGMD2A should not be excluded in siblings on the basis of absence of symptoms alone. Instead, evaluation of creatine kinase level in serum seems an adequate screening method, if clinically indicated.

Acknowledgments

First of all the Authors thank the individuals and their families reported here.

JS, MCW, US, CRM, HL, CGB, RK and JK are members of the German network on muscular dystrophies (MD-NET, 01GM0601) funded by the German Ministry of Education and Research (BMBF, Bonn, Germany). MD-NET is s partner of TREAT-NMD (EC, 6th FP, proposal #036825).

Disclosure

Disclosure

Manuscript prepared without any financial support or any kind of financial interests.

References

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Articles from Acta Myologica are provided here courtesy of Pacini Editore