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Lungs of 40 ruminants from Bulgaria with natural small lungworm (Nematoda: Protostrongylidae) infections were investigated, including 16 goats, 15 sheep, 7 mouflons, and 2 chamois. Muellerius capillaris, M. tenuispiculatus, Cystocaulus ocreatus, Neostrongylus linearis, and Protostrongylus brevispiculum infections were predominantly associated with nodular lesions, and Protostrongylus rufescens, Protostrongylus hobmaieri and Protostrongylus rupicaprae were associated with extensive lesions located mainly along the length of the large bronchi. The extent of lung abnormalities was most severe in the sheep. Alveolitis, parasite granulomas, damage of the alveolar septa, hyperplasia of the lung associated lymphoid tissue, and sclerosis of the parenchyma were found upon microscope examinations. In the goats compared to the sheep and mouflons, the terminal bronchi, bronchioles, and alveoli were more affected than the interstitium. Our research shows that the pathological lesions in the lungs of ruminants infected with protostrongylids depend on both the helminth and the host species. To our knowledge, this work is the first to provide data on the pathomorphological lesions in mouflon lungs infected with protostrongylids.
Protostrongylidoses are widespread helminthoses in domestic and wild ruminants causing a serious health problem for a great number of countries all over the world. Their etiological agents are the so-called small lungworms (Nematoda: Protostrongylidae). These infections are mainly associated with respiratory disturbances [1, 2], development of focal pneumonia [3, 4], and secondary bacterial infections of the lungs [5–7]. Loss of weight, lower animal productivity, reduced numbers of offspring, abortions, or neonatal deaths, and increased mortality [3, 8–11] have also been linked with many protostrongylidoses. The pathologic features of these ailments have been studied in detail, but the data obtained show that various kinds of inflammatory reactions can be found within the lesions. Therefore, based on the fact that the types of lung damage produced by protostrongylids are numerous and vary in severity and extent in the different studies [8, 12–19] the aim of the present study was to analyze the most frequent pathological lesions in the lungs of goats, sheep, mouflons, and chamois from Bulgaria attributed to different protostrongylid species in order to achieve a more detailed characterization of the particular parasitic infections in the different hosts.
Lungs of forty ruminants from different regions of Bulgaria, including 16 goats (Capra aegagrus f. domestica L.), 15 sheep (Ovis ammon f. domestica L.), 7 mouflons (Ovis musimon L.) and 2 chamois (Rupicapra rupicapra L.), were investigated. The lungs of the domestic ruminants were collected during standard postmortem examination at several slaughter houses from Veliko Tarnovo and Sofia provinces. Information about the animals' age was obtained by the owners of the flocks. The lungs of wild ruminants were obtained after selective hunting of animals inhabiting wildlife-breeding reserves in Dobrich and Smolyan provinces. All procedures were approved by the Institute of Experimental Pathology and Parasitology Ethics Committee and complied with the requirements of Ordinance No 22/14.12.2005 on reducing to a minimum suffering of animals at the time of slaughter or killing (Promulgated in SG 22 of 14 Dec 2005 and transposing Directive 93/119/EC on the protection of animals at the time of slaughter or killing) and European Union guidelines for the accommodation and care of animals used for experimental and other scientific purposes (2007/526/EC). The materials were transported immediately to the laboratory with the exception of 20 lungs (8 of goats, 5 of sheep, 5 of mouflons, and 2 of chamois) which were deep frozen and transported later.
Firstly, a topographic sketch of the superficial abnormalities in the lungs was drawn. After that routine necropsies of the lungs were performed. The helminths within the particular lesion were found and their species were determined under the microscope. Aiming to find the small, hardly visible worms located in the bronchioles and alveoli, the abnormal lung tissues were examined as follows: 1-2cm3 parts from the lung lesions were selected and boiled in 40% lactic acid in a water bath for 1.5hour. After that small (2-3mm) pieces of those parts were compressed and observed under a light microscope. In this way the lungworms became brighter and sexual structures of the male specimens were clearly visible. The identification of the helminths observed was carried out on the basis of their morphometric characteristics .
The locality, consistency, colour, formation of nodules, presence of abscesses, presence of other nonparasitic lesions, and dissemination were taken into consideration during the evaluation of the macroscopic lesions in the infected lungs. According to their dissemination, macroscopic lesions were subjectively classified as follows: slight (small, hardly visible lesions), mild (affecting up to 1/6 of the lung surfaces), severe (affect from 1/6 to 1/2 of the lung surfaces), and very severe (affecting over 1/2 of the lung surfaces).
Small organ pieces (0.5 × 2 × 2cm) were obtained from the helminthologicaly analyzed (but not boiled) tissue lesions and processed for histopathological examination. They were fixed in 10% phosphate buffered formalin, embedded in paraffin, cut into sections of 5 to 10μm thick, and stained with haematoxylin and eosin according to standard technique.
The parasitic lesions established in the present study were located within the caudal lung lobes and were disseminated mainly within the dorsal subpleural parenchyma. The results of the macroscopic examinations as well as the species of the hosts and protostrongylids established in each animal are presented in Tables Tables1,1, ,2 and2 and and33.
The lung lesions in goats infected with M. capillaris were nodular, firm, and gray. In the younger animals small, grayish areas on the dorsal surface of the caudal lung lobes were found. Rarely larger congested, gray areas, situated close to the lung pleura, were observed in the goats. The lesions in the lungs of sheep infected with M. capillaris were more severe, regardless of the animals' age. In the most cases they were not formed as nodules and were firm, grey to black areas affecting a large part of the lung surfaces. Diffuse congestion of the lung tissue was found when those abnormalities were cross-sectioned. The lesions in the lungs of mouflons infected with M. capillaris were of several types—grey patches on the lung surfaces of different size, extensive, diffuse, dark-grey to black congested areas (Figure 1(a)), and well-formed, firm, grey nodules (Figure 1(b)). Small hard nodules of a diameter of 1–5mm under the lung pleura also were observed in the mouflon lungs (Figure 1(c)). Nodular lesions were observed in the lungs of chamois and sheep infected with M. tenuispiculatus, N. linearis, and P. brevispiculum. Adult C. ocreatus were found in nodules having a different structure (Figure 1(d)). They were well differentiated from the surrounding tissue, slightly prominent above the serous surface of the visceral pleura, but not very dense and of normal or soft consistency. They contained internal cavities filled with a caseous substance. The macroscopic abnormalities in the lungs of ruminants infected with larger species of the genus Protostrongylus consisted primarily of extensive congestion of the tissue along the largest bronchi and were dark-red to grey (Figure 1(e)). In these cases adult helminths were found in the dissected bronchi (Figure 1(f)).
The results of the histological investigations, concerning abnormalities in the bronchi, alveoli, and alveolar septa as well as the progress of specific changes in the lung parenchyma and interstitium around the adult worms, eggs, and larvae of the parasites are shown in Table 4.
The abnormalities in the lungs of goats varied in severity in the affected lobules and acini. The bronchi and bronchioles contained desquamated epithelial cells, alveolar macrophages, neutrophils, and parasite forms in different stages, sometimes encircled by slight lymphoid hyperplasia (Figures 2(a) and 2(b)). Many alveoli also contained a great number of parasite forms (eggs and first stage larvae) and inflammatory exudate consisting of alveolar macrophages and neutrophils. However, in many of these cases a thickening of the alveolar septa was not observed (Figure 2(c)).
Similar abnormalities were found in the lungs of sheep and mouflons. Additionally, the thickening of the alveolar septa as a consequence of hyperplasia of the connective tissue and smooth muscle cells was regularly observed (Figure 3(a)). Parasite granulomas consisting of a necrotic centre, calcification, peripheral lymphoid hyperplasia, and giant cells were observed frequently (Figures 3(b) and 3(c)). Development of serous, giant cell, and macrophage alveolitis was demonstrated (Figures 3(d), 3(e), and 3(f)). In sheep and mouflons, marked hyperplasia of the lymphoid tissue was also observed. Hyperplastic lymphoid structures were situated both close around the bronchi and in the lung interstitium and sometimes were spread to whole lobules (Figures 4(a), 4(b), and 4(c)). In some animals, sclerosis of the parenchyma with a lymphocyte infiltration of the sclerotic areas was observed (Figure 4(d)).
Many pathological features of the lungs in domestic and wild ruminants with protostrongylid infections observed in the present study have been described by other researchers [8, 13, 14, 16, 17, 19–24]. However, limited attention has been paid to the parasite-host interaction within the inflammatory foci. The inflammatory response and the variety of lymphoid hyperplasia observed in the present study clearly indicate that interesting protostrongylid-host interactions are taking place in the protostrongylid infected lungs.
Comparing our results with the data from other studies in goats with protostrongylid infections we find both similarities and differences. Our observations confirm Svarc's  conclusions that the application of infective M. capillaris larvae causes a nodular form of muelleriosis in goats but it can be associated also with an exudative reaction. To a certain extent our results coincide with those of Nimmo  who found dark coloured areas mostly on the dorsal surfaces of the caudal lung lobes in 2-year-old animals with a Muellerius sp. infection. Such lesions we observed in kids under 6 months old. In older goats we observed the formation of nodules, which is missing according to Nimmo . The author  found only congestion of the lung tissue and asserts that diffuse interstitial pneumonia is the most obvious histological change in goats with Muellerius sp. infection, although according to other studies that finding is typical mostly of sheep. Nimmo  associates the diffuse interstitial pneumonia with thickening of the alveolar septa, which is due to fibromuscular hyperplasia and mononuclear cell infiltration. However, according to our data many of the alveolar septa remain without visible morphological abnormalities despite the presence of a large number of parasites in the alveolar lumen. According to the results of Valero et al.  the lesions in goats associated with M. capillaris infection are nodular, disseminated mostly on the caudo-dorsal aspects of the lungs and their prevalence increase with age. The data of Berrag et al.  are similar. Histologically Berrag et al.  describe a variable degree of inflammatory cell reaction. In that respect their data are in accordance with ours. Regarding the macroscopic lesions our results were similar to those of Berrag and Cabaret  who ascertain nodules in the case of M. capillaris infection and lobular lesions in P. rufescens infection. With respect to the dissemination of the lung abnormalities in animals of a particular age category, however, our results were different. Berrag and Cabaret  indicate that the surface of the lungs covered with protostrongylid lesions is larger in kids than in older goats but we found the opposite. That difference most likely is due to the increased sensitivity of individual nonimmune young animals investigated by the authors  affected for the first time in their life with a massive protostrongylid infection.
Our studies show that the lesions in the sheep with M. capillaris infection are rarely nodular and represent extensive, diffusely congested, grey areas. There were some differences in the macroscopic lung lesions in goats and sheep. The lesions in sheep grew predominately into deep lung tissue whereas those in the goats were more superficial. Formation of firm, distinct, prominent nodules in sheep were observed mainly due to N. linearis and P. brevispiculum infections. As a whole the lungs of sheep were affected to a greater degree than those of the goats. The cases of slight and mild lung abnormalities in the sheep were few even in young animals. Severe abnormalities predominated and mixed infections were often connected with very severe abnormalities. Our results show that the microscopic abnormalities in the sheep lungs with M. capillaris infection are similar to those in goats. Furthermore, in sheep, thickening of the alveolar septa and formation of parasite granulomas varying in cell structure were observed. In the mixed infections, giant cell alveolitis and marked hyperplasia of the lymphoid tissue were observed. Beresford-Jones  describes irregularly disseminated, prominent, yellow-grey, diffuse lesions under the lung pleura penetrating into the parenchyma as well. These data support our results that the lung lesions in sheep with M. capillaris infection are mostly of a diffuse rather than nodular character. There is a similarity in the histological picture too. A thickening of the alveolar septa in the lungs of sheep with M. capillaris infection is observed both in the studies of Beresford-Jones  and in ours. Thomas et al.  found that 98% of lung lesions in sheep with M. capillaris infection affect the caudal lobes. However, they describe the established lesions as nodular and do not mention the more widespread ones which we found in many of the cases. Our results were in accordance with Svarc  who points out the presence of both types of lesions as well. According to Bouljihad et al.  the lesions in the lungs infected with M. capillaris and P. rufescens appear as red to brownish thickened areas. The lesions so described are similar to our findings only with regard to infection with P. rufescens, lesions observed also by Mansfield and Gamble  in sheep with a P. rufescens infection. Nodular lesions and the presence of capsules around them attributed to C. ocreatus infection also have been established by scanning electron microscopy .
The lesions in the lungs of mouflons with M. capillaris infection were both diffuse and nodular and in this case it was difficult to determine which predominated. Lesions associated with N. linearis, C. ocreatus, and P. rufescens infections were analogous to those in sheep. The lesions associated with M. capillaris in our work were similar to those established by Demartini and Davies  in other wild sheep species (Ovis canadensis L.) from Colorado in the United States. More typical findings in the mouflons' lungs were the small, clearly differentiated, very hard, grayish nodules under the lung pleura. Many hard, crystal-like structures and helminth parts were observed in these nodules under compression after boiling in lactic acid. We are unaware of data in the literature about the pathological lesions in mouflons with protostrongylid infections. The nodules mentioned above, however, were rather similar to the granulomas described by Beresford-Jones  in sheep infected with M. capillaris while McFadyean 1894 (citation after Beresford-Jones ) describes them as pseudotubercules. According to the criteria subjectively adopted by us the lung abnormalities in mouflons were categorized as mild. Severe abnormalities were observed only in one case of a mixed infection with 4 protostrongylid species. Very severe abnormalities were not found. The microscopic abnormalities in the mouflons were similar to those in sheep. The abnormalities in the bronchi were more frequent in the presence of P. rufescens infection. Often, in mixed infections, neutrophilic-macrophagic alveolitis and hyperemia of the septa were found. The granulomas formed had large areas of calcification and macroscopically corresponded to the small, hard nodules under the lung pleura.
The macroscopic lung lesions in the chamois were diffuse in the mixed P. rupicaprae and N. linearis infection and nodular in the M. tenuispiculatus and N. linearis infection. Small hard nodules such as those described in mouflons were found in chamois too. As a whole the lesions established by us were similar to those described in chamois by Svarc [26, 27] and Iacob et al. .
According to Stockdale, 1976, and Chitwood and Lichtenfels, 1972 (citation after Valero at al. ) different species of protostrongylids produce similar gross and microscopic lesions. However, our studies show some unique aspects of the pathomorphological lung lesions in ruminants depending on the nematode and host species. These lesions were analyzed in view of refining and correlating the pathomorphological and parasitological criteria required for the diagnosis of those infections and can be summarized as follows.
In summary, our research shows that the pathological lesions in the lungs of ruminants infected with protostrongylids depend both on the helminth and the host species.