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Vertebrate heterochromatin contains a non-allelic variant of the histone H2A called macroH2A1, which has the characteristic of being three times the size of the canonical H2A. The macroH2A1 C-terminal extension can recruit onto chromatin the poly-ADP-ribose polymerase (PARP)1, which is crucial for DNA repair. This led to the speculation that macroH2A1 could be essential for genome surveillance; however, no experimental evidence supported this hypothesis. Because macroH2A1 has been found to be enriched on the inactive X-chromosome in females, it is thought to play a role in sex chromosome dosage compensation through its ability to regulate gene expression. However, more genetic data are needed to further understand the function of macroH2A1 in mammals.
Deletion of the murine gene H2afy, which encodes for macroH2A1, resulted in lipid accumulation in liver. Hepatic steatosis caused by H2afy disruption occurred specifically in homozygous mutant females. The metabolic disorder constantly affected half of the number of homozygote females. Given the mixed genetic background of the mutants, an unreported genetic modifier is likely to influence the penetrance of the phenotype. In addition, the X-linked thyroxine-binding globulin (Tbg) gene was specifically upregulated in steatotic livers. Chromatin immunoprecitation indicated that macroH2A1 is enriched at the Tbg promoter in wild-type female animals, indicating that increased Tbg expression in H2afy null mutants is likely to be a direct consequence of the absence of macroH2A1. Furthermore, male mice, which are not prone to the metabolic disorder, had a reduced level of macroH2A1 incorporated into the Tbg promoter.
Because TBG is the main carrier of the thyroid hormone T4, which regulates energy metabolism, we propose that overexpression of TBG is responsible for the fat accumulation observed in H2afy-deficient liver. Moreover, our results suggest that the sexual dimorphism of the steatotic phenotype is probably due to the different incorporation of macroH2A1 in males and females. In combination with previous studies, our data demonstrate a role for macroH2A1 in regulating homeostasis in a sex-dependent manner, subject to genetic background.
The histone variant macroH2A1 has the ability to substitute for H2A within the nucleosome and to establish chromatin domains with specific properties, due to its C-terminal non-histone macrodomain . Incorporation of macroH2A1 results in the addition of a C-terminal domain of 25 kDa to a histone fold motif, and thus constitutes the largest known nucleosome modification . MacroH2A1 dynamic recruitment onto the inactive X-chromosome (Xi) upon inactivation in female mammals indicates a possible function in sex-chromosome dosage compensation [2-5]. However, quantities of macroH2A are similar in males and females, suggesting that a role in X inactivation is not the sole function of this variant .
The presence of macroH2A1 at promoter regions was shown to inhibit gene expression by in vitro transcription analysis of reconstituted nucleosomes containing macroH2A1 [7-9]. However, knockdown studies performed in cultured cells demonstrated that macroH2A1 is required for both transcriptional silencing  and induction [11,12]. This dual role of macroH2A1 in transcription was investigated further in a recent study, which showed that 12% of the genes enriched in macroH2A1 escape silencing . Furthermore, the same study showed that a subset of genes is downregulated upon macroH2A1 knockdown, indicating that macroH2A1 could act as a positive regulator of transcription at some loci .
In vivo, the presence of macroH2A1 is essential for the silencing of a class of endogenous (non-ecotropic) murine leukaemia viruses . In addition, macroH2A1 plays a function in NAD+ catabolism through its role in the two main pathways of NAD+ consumption. First, macroH2A1 binds to poly-ADP polymerase (PARP)-1 and inhibits its enzymatic activity [12,15,16]. PARP-1 is a nuclear enzyme that catalyzes the cleavage of NAD+ into ADP-ribose and nicotinamide, and the subsequent transfer of ADP-ribose units to target proteins . PARP-1 is implicated in chromatin structure modulation, transcription regulation and double-strand break (DSB) DNA repair . Secondly, macroH2A1 binds the metabolite O-acetyl-ADP-ribose produced by the NAD+ dependent histone deacetylase Sirt1 . Although all these recent findings provide biochemical evidence indicating that macroH2A1 plays a role in NAD+ catabolism, genetic evidence is needed to identify its function in energy metabolism in mammals.
In this study, we generated loss-of-function animals through targeted disruption of H2afy, the gene that encodes macroH2A1. Mice that lacked macroH2A1 displayed no obvious visible phenotype and no increased sensitivity to ionizing radiation, which excludes an essential function for macroH2A1 in genome surveillance. However, we found that macroH2A1-deficient females exhibit liver lipid homeostasis defects that lead to hepatic steatosis. Interestingly, lipid accumulation in the liver was specific to females. We provide evidence of reactivation of the X-linked thyroxine-binding globulin (Tbg) gene specifically in steatotic livers. We also demonstrate that the histone variant macroH2A1 is specifically enriched in nucleosomes that occupy the Tbg promoter in female hepatocytes, indicating that Tbg overexpression is a direct consequence of H2afy disruption.
We generated an H2afy null-mutant mouse strain by homologous recombination in ES cells. Our strategy resulted in the complete disruption of exon 2, which encodes the translation start codon and approximately half of the histone domain (Figure 1a, b).
Mating of heterozygous animals resulted in offspring of all genotypes at the mendelian ratio (95 heterozygous, 68 wild types and 48 homozygous) and the overall sex ratio was close to 1:1. Mice homozygous for the H2afy mutation were viable, and survived to adulthood without discernible morphological abnormalities. Both male and female H2afy-/- mutants were fertile and produced litter sizes comparable with the wild type.
Absence of the macroH2A1 protein in tissues was confirmed in homozygous mice by immunoblotting using a specific antibody (Figure (Figure1c).1c). To determine whether compensation might be involved, the expression level of macroH2A2 (H2afy2) was examined in different tissues. MacroH2A2, which is 80% identical to macroH2A1, is encoded by a separate gene located on chromosome 10 [19,20]. Little is known about this second macroH2A subtype, but it was recently shown to be crucial for zebrafish (Danio rerio) embryogenesis . In contrast to many human cells and mouse, zebrafish embryos predominantly express H2afy2, whereas H2afy was not detectable .
No overexpression of H2afy2 was observed in the absence of macroH2A1 (Figure (Figure1c).1c). Therefore, it is unlikely that the absence of a phenotype in H2afy-deficient animals is the result of a functional compensation by H2afy2.
Because macroH2A1 interacts with PARP-1, which is essential for early DNA damage recognition and base excision repair by sensing DNA nicks , it has been proposed that macroH2A1 could play a role in the process of DNA repair through its ability to recruit PARP1 onto chromatin [12,16].
To determine whether macroH2A1 is essential for DSB repair in vivo, we tested the sensitivity of H2afy-deficient mice to whole-body ionizing sublethal irradiation. Mice lacking macroH2A1 did not show any increased sensitivity to gamma radiation compared with control littermates (Figure (Figure2).2). Histological sections through the duodenum did not show any difference in the size of villi between mutant and control mice (data not shown), indicating normal ionizing radiation-induced cell renewal. We conclude that macroH2A1 is not required for accurate DNA break repair in vivo.
Histological examination of all major organs, including brain, kidney, spleen, heart, visceral fat and reproductive organs, showed no differences between macroH2A1-deficient mice and control littermates up to 12 weeks of age. However, analysis of liver from H2afy-/- females revealed signs of hepatic steatosis (Figure (Figure3,3, Table Table1).1). Of 17 mutant females, nine displayed periportal hepatocellular macrovacuolation (Figure 3a, b), a phenotype that was not observed in any mutant male or in wild-type male and female mice. We found by staining tissue sections with oil red O that this vacuolation was the result of abnormal triglyceride accumulation in liver (Figure 3c, d). Hence, we conclude that the absence of macroH2A1 induces steatosis.
Incorporation of macroH2A1 in chromatin has been shown to be required for the silencing of the endogenous retrovirus murine leukemia virus (MLV) . To explore a possible correlation between MLV overexpression and hepatic steatosis, we assessed MLV env transcription in H2afy-/- female steatotic livers compared with H2afy-/- female healthy livers and wild-type female livers (Figure (Figure4a4a).
Transcription of MLV elements was not detected above background level in three of four wild-type samples. Surprisingly, an MLV transcript was detected in one wild-type sample (Figure (Figure4a),4a), showing that MLV can also be transcribed in the presence of macroH2A1; this may reflect the segregation of a gene unlinked to H2afy that regulates MLV expression. Quantification of the northern-blot data confirmed that MLV silencing is lost in the absence of macroH2A1, as previously reported . MLV expression level, normalized to GAPDH, was upregulated by 2.3-fold in H2afy-/- mutants (Figure (Figure4b),4b), but no significant difference was observed between healthy mutant livers and steatotic livers (Figure (Figure4b).4b). We therefore exclude a relationship between hepatic steatosis and MLV overexpression in the absence of macroH2A1.
A major cause of hepatic steatosis is the increased fatty acid flux to the liver caused by a high level of free fatty acids in plasma . To gain further insight into the molecular causes of the triglyceride accumulation in the absence of macroH2A1, we investigated a possible correlation between this phenotype and the expression level of a few genes important for lipid metabolism that have been previously shown to be regulated by macroH2A1  (Figure (Figure55).
The expression level of lipoprotein lipase (Lpl), which catalyses the hydrolysis of the triacylglycerol component of circulating chylomicrons and very low-density lipoproteins , was not affected by the mutation (Figure (Figure5b).5b). Mice lacking macroH2A1 presented an upregulation of CD36 (Figure (Figure5a),5a), a fatty acid transporter in muscle and adipose tissue. By contrast, Thrsp (thyroid hormone responsive SPOT14 homolog), a regulator of de novo lipogenesis, was downregulated in H2afy-/- livers compared with controls (Figure (Figure5c)5c) . Thus, our results suggest that Thrsp belongs to the recently discovered category of genes that are positively regulated by macroH2A1 .
No differences in Thrsp or CD36 expression were observed between H2afy-/- females showing a steatotic liver and those with a healthy liver (Figure 5a, c). Therefore, it is unlikely that the steatotic phenotype observed in H2afy-/- females was a consequence of the deregulation of CD36 and Thrsp expression.
Remarkably, the X-linked Tbg gene (also named Serpina7) was overexpressed by sixfold in H2afy-/- female mice with a steatotic phenotype compared with healthy mutant or wild-type females, which both expressed Tbg at the same level (Figure (Figure5e).5e). TBG is a thyroid hormone-binding protein, which carries the majority of the hormone T4 in the serum . Given that thyroid hormones influence general energy metabolism and specifically promote increases in lipid metabolism and counteract lipid accumulation , overexpression of Tbg in H2afy-/- females and its consequences on lipid metabolism could be the origin of the hepatic steatosis we observed.
To test if the overexpression of Tbg was a consequence of a global reactivation of Xi in the absence of macroH2A1, we examined expression of Dlg3, another gene located on the X-chromosome (Figure (Figure5d).5d). Although the expression level of Dlg3 was slightly elevated in mutant mice, Dlg3 did not show any significant upregulation in steatotic liver (Figure (Figure5d).5d). Together, these data indicate that the inactive X-chromosome is not fully reactivated in the absence of macroH2A1, indicating that macroH2A1 is not required for global silencing of Xi.
To determine if macroH2A1 directly controls the expression of genes most perturbed in H2afy-/- liver, we performed chromatin immunoprecipitation (ChIP) experiments to test for the presence of macroH2A1 in nucleosomes that occupied promoters of those genes in livers of wild-type animals (Figure (Figure66).
We first analysed chromatin extracted from female livers (Figure (Figure6a).6a). We did not detect the presence of macroH2A1 above the background level on the Lpl gene, which was not deregulated in the absence of macroH2A1 (Figure (Figure5b).5b). By contrast, wild-type female hepatocytes displayed high levels of macroH2A1 incorporated into the sequence upstream of the Tbg transcription start site (Figure (Figure6a).6a). This finding strongly suggests that increased expression of Tbg measured in H2afy-/- livers is a direct consequence of H2afy disruption.
To determine the molecular cause of the sexual dimorphism of the steatosis promoted by H2afy disruption, we compared macroH2A1 levels on the Tbg promoter in male versus female animals (Figure (Figure6b).6b). Consistent with our first analysis (Figure (Figure6a),6a), we found that macroH2A1 is 10-fold enriched on the Tbg promoter in females (Figure (Figure6b).6b). Furthermore, our ChIP analysis shows that in contrast to females, the Tbg promoter in males has low amounts of bound macroH2A1 (Figure (Figure6b).6b). It should be mentioned that in a previous study , this sex-specific difference was not noted. This might be the result of differences in the purification method of macroH2A containing nucleosomes (immunoprecipitation in this study; biochemical purification in the previous study ). However, in the previous study, Tbg expression was shown to be four times higher in the livers of wild-type adult males than in wild-type adult females . Hence, the sexual dimorphic expression of Tbg in wild-type mice is probably caused by the low incorporation of macroH2A1 on the Tbg promoter in male hepatocytes.
We conclude that the sexual dimorphism of the phenotype caused by H2afy deletion is caused by the sex-dependent incorporation of macroH2A1 at specific loci, as evidenced by the sex-specific incorporation of macroH2A1 on the Tbg promoter.
Our study provides novel insights into macroH2A1 functions in X-chromosome inactivation and lipid metabolism. Loss of function of H2afy causes hepatic accumulation of lipid specifically in female mice.
Interestingly, a recent report has demonstrated that mice fed with a methyl-deficient diet that induces hepatic steatosis had an elevated level of macroH2A . Although this seemingly contradicts our results, we propose that the elevated level of macroH2A reported in this study reflects a homeostatic response within the liver that is ultimately saturated after prolonged exposure to high levels of lipids. In the light of this study , our findings indicate that macroH2A could be incorporated at high levels in chromatin in order to circumscribe hepatic lipid accumulation.
Using a similar approach, Changolkar and colleagues have independently shown that H2afy-deficient mice are viable, and that the mutation does not result in any obvious macroscopic phenotype . Those independent data consistently demonstrated that macroH2A1 does not play a fundamental role during development; however, two recent studies have shown that macroH2A1 is preferentially incorporated at developmental gene promoters [13,21]. This apparent contradiction might be explained by the potential existence of redundant chromatin silencing pathways. One hypothesis suggests functional compensation by the polycomb repressive complex (PRC)2, as indicated by its colocalisation with macroH2A1 . This hypothesis is also sustained by the genome-wide colocalisation between macroH2A1 and the trimethylation of lysine 27 of histone H3, the histone posttranslational modification catalysed by PRC2 [13,30]. However, the possible redundant roles of PRC2 and macroH2A1 remain to be experimentally tested.
In addition, the study of Changolkar et al. showed that H2afy disruption has a minor influence on gene expression, as only seven genes displayed significant abnormal expression in the absence of macroH2A1 . The surprisingly low number of genes affected by the macroH2A1 deletion could be explained either by possible functional compensation by another redundant chromatin repressive mechanism or by the presence of genetic modifiers, as discussed below. Very interestingly, four genes deregulated in H2afy-/- livers encode proteins directly involved in fatty acid metabolism, which further supports the role of macroH2A1 in lipid homeostasis control as highlighted in our study. We have independently confirmed the abnormal upregulation of three of these genes in H2afy-/- liver. However, we found a decreased level of Thrsp in mutant female liver, whereas the study of Changolkar et al. concluded that Thrsp expression is not affected by H2afy disruption in female hepatocytes .
The major discordance between these two parallel studies concerns liver steatosis; there is no mention of this in the study from Changolkar et al. . This important difference could be explained by the different genetic backgrounds of mice in these two studies. The study by Changolkar et al. was performed using a fixed mutation on the C57Bl6 background whereas the mutants we analysed had a genetic background composed of half 129Ola and half C57Bl6 (intercrosses of the F2 generation). Such interference of the genetic background with the phenotype has been extensively documented for many genes . Likewise, variation of the genetic background has been reported to have significant influences on both the quantities of macroH2A1 in steatotic liver and the incidence of steatosis .
Additionally, our data reveal that the penetrance of the steatotic phenotype of female progenies of C57Bl/6 × 129Ola mice was very close to half. This consistent penetrance in half the animals over several intercross generations indicates the existence of a genetic modifier present in one of the two mixed genetic backgrounds that is able to modify the penetrance of the phenotype resulting from H2afy deletion. Identification of this genetic modifier should expand our knowledge of the metabolic pathway controlled by macroH2A1 in female.
Our study uncovers part of the molecular mechanism by which macroH2A1 regulates lipid homeostasis. Indeed, we show that macroH2A1 silences Tbg expression in female liver, as evidenced by the overexpression of Tbg in the absence of macroH2A1. We also provide evidence of specific enrichment of macroH2A1 on the Tbg promoter, which indicates that macroH2A1 directly regulates Tbg transcription.
The consequences of Tbg overexpression in mice are unknown. However, studies in patients with amplification of the Tbg gene revealed that excess TBG can lead to hypothyroidism and altered metabolism [32,33]. Thus, we propose that elevated levels of TBG, the main T4 hormone carrier, could be the cause of the alteration in lipid metabolism responsible for hepatic steatosis in the absence of macroH2A1.
The reactivation of the X-linked Tbg caused by H2afy disruption raises the possibility that the remarkable sexual dimorphism of the phenotype might be due to Xi silencing failure in the absence of macroH2A1. However, we found that expression of Dlg3, another X-linked gene, is not affected by H2afy disruption. Therefore, Xi is not entirely reactivated in the absence of macroH2A1. In good agreement with our conclusion, a global analysis of gene expression in H2afy-/- liver did not report evidence of Xi reactivation in the absence of macroH2A1 . This notion was reinforced by a previous study, which reported that H2afy knockdown is not sufficient to reactivate the transcription of a reporter gene stably inserted in Xi .
Given the low level of macroH2A1 incorporated into the male Tbg promoter, we propose that the sexual dimorphism of the phenotype could be due to the selective incorporation of macroH2A1 on the Tbg promoter in female cells. Our measurements show that the amount of macroH2A1 incorporated into the Tbg promoter is about fivefold higher in female mice than in male mice. This 5:1 ratio cannot be explained by the twofold difference of the X-chromosome copy number between the sexes. Instead, the difference in macroH2A1 occupancy at the same locus between males and females is probably caused by an unbalanced incorporation of macroH2A1 between the sexes. Specific enrichment on the female X-linked Tbg promoter could possibly be a reflection of the specific incorporation of macroH2A1 on Xi [2-4,35]. The different levels of macroH2A1 on the Tbg promoter locus between the sexes might also be a consequence of a more general differential genomic localisation of histone variants between different cell types (for example, male versus female).
Although much effort has been devoted to understanding the role of macroH2A1 in gene silencing, its biological function remains elusive. Recent advances that uncovered its association with PARP-1 have led to the speculation that macroH2A1 could be involved in DNA repair. However, we report here that H2afy-/- mutants are not hypersensitive to DNA DSB, excluding a fundamental role of macroH2A1 in the control of the DNA damage response.
In addition, we have shown that loss of function of H2afy causes hepatic accumulation of lipid specifically in females. We also report a correlation between macroH2A1 occupancy on the Tbg promoter and its specific overexpression in steatotic liver, which strongly supports our hypothesis that Tbg deregulation is the major cause of lipid metabolism dysfunction in the absence of macroH2A1. Thus, our study provides novel insights into macroH2A1 functions in X-linked gene silencing and lipid metabolism.
The importance of chromatin in the control of homeostasis has recently begun to be uncovered with the discovery of the role of histone deacetylase and histone demethylase in the regulation of energy metabolism [36,37]. Our work highlights that histone variants are also involved in the regulation of lipid metabolism.
All experiments on mice were carried out with CREA Rhône-Alpes (France) committee approval. Mice were normally housed in cages with littermates of the same sex. Animals were kept in a 12-hour light/dark cycle and fed standard diet rodent chow (SAFE #A04-10 and SAFE #A03-10 for breeders; Perotech Sciences Inc., Toronto, ON, Canada).
H2afy was disrupted by replacing exon 2 with a neomycin resistance cassette. Both recombination fragments were first amplified using DNA polymerase (Phusion; New England BioLabs, Ipswich, MA, USA) and appropriate primers (Table (Table2)2) on DNA extracted from E14.1 ES cells, then cloned into SalI and XhoI restriction sites, respectively, of the pLN-TK targeting vector (Figure (Figure1).1). The resulting vector was linearized with MluI and electroporated into E14.1 ES cells (129/Ola strain) as described previoulsy . After antibiotic selection (G418/Geneticin; Gibco-BRL, Grand Island, NY, USA), homologous recombinants were identified by PCR using the set of primers named 'screen2 F' and 'NeoLOXP2 R' shown in Table Table2.2. Homologous recombination was confirmed by Southern blotting and extensive restriction analysis, using flanking and internal probes (data not shown). Two different ES clones heterozygous for the targeted mutation were injected into C57Bl/6 blastocysts and transferred into pseudopregnant foster mothers. One clone gave high-level chimeras and transmitted the disrupted H2afy allele to offspring at high frequency when crossed into C57Bl/6 mice. The mice were maintained by intercrosses of the original 129Ola × C57Bl/6 mice. The animals were genotyped by PCR on genomic DNA isolated from finger biopsy, using the primer pairs 'screen F' located in intron 1-2 and 'screen R' located in the targeted portion of intron 2-3, and a third primer complementary to the neo gene 'NeoLOXP2 R' (Table (Table2).2). This resulted in the amplification of a 500 bp fragment from the wild-type macroH2A1 allele and a 310 bp fragment from the null allele.
Nuclear proteins were isolated from tissues in accordance with the protocol described previously . Briefly, 10 μg of nuclear proteins were separated by SDS-PAGE and transferred onto nitrocellulose membranes. For the detection of macroH2A1, a polyclonal antibody (Millipore Inc., Billericay, MA, USA) was used. MacroH2A2 was detected using a rabbit polyclonal antibody (cat. no. ab4173; Abcam Inc., Cambridge, MA, USA) at a dilution of 1:2000. Histone H2B was detected using a rabbit polyclonal antibody (cat. no. 07-371; Millipore) at a dilution of 1:5000 .
For Southern blotting, 10 μg of DNA were digested with 100 U of EcoRI, then separated by agarose gel electrophoresis and transferred onto a nitrocellulose membrane, followed by UV crosslinking. Filters were hybridized with a 32P-labelled probe located 5' to the sequences in the targeting vector (Figure (Figure1).1). Membranes were washed at 65°C, twice with 1× saline sodium citrate (SSC) buffer plus 0.1% SDS for 15 minutes each, and twice with 0.1× SSC plus 1% SDS for 30 minutes each, then exposed to a phosphor screen for 24-72 hours. The gel images were acquired with a scanner (Fujifilm 217 FLA-5100; Fujifilm Holdings Corp., Tokyo, Japan) and the pictures were analyzed with Multigauge V.3.0 software (Fujifilm).
For induction of DNA damage, 7-8-week-old H2afy-/- mice and control littermates were exposed to whole-body sublethal ionizing irradiation. Animals were irradiated with a 137Cs source at a rate of 0.68 Gy/min in single doses of 6.5 Gy. H2afy-deficient mice and seven control littermates were irradiated, and survival was determined. Four days after irradiation, one mutant and one wild type were killed for histopathological analysis of the small intestine.
Formaldehyde-fixed, paraffin wax-embedded sections were prepared from liver samples, and were stained with hematoxylin and eosin using standard methods. Frozen liver sections were stained with oil red O. Sections were photographed at 20× magnification.
Total RNA was isolated using TRIzol reagent (Invitrogen Corp., Carlsbad, CA, USA) and treated with DNaseI, then 5 μg of RNA were reverse transcribed (RevertAid Reverse Transcriptase; Fermentas, Burlington, ON, Canada). Quantitative reverse transcriptase (RT) PCR reactions were performed using the Light-Cycler system (Roche Applied Science; Indianapolis, IN, USA) to determine mRNA levels of Tbg, Lpl, CD36, Thrsp and Dlg3 (specific primers are listed in Table Table2).2). All cDNAs were normalized using primers to Gapdh. Expression levels were corrected against expression measured in control littermate liver, which was defined as 1. cDNA levels from wild-type (n = 2), steatotic macroH2A1-/- (n = 4) and healthy macroH2A1-/- (n = 3) were compared, and results expressed as mean ± SEM.
For Northern blotting, 10 μg of total RNA were denatured and subjected to electrophoresis in a 1% agarose plus 1.9% formaldehyde gel in 1× MOPS buffer, and transferred to a nitrocellulose membrane. The membrane was hybridized with probes derived from radiolabeled extension of random hexamers annealed to the purified 1011 bp fragments produced by MelARVenv digestion by NcoI (described in ) or Gapdh previously amplified from cDNA using the primers listed in Table Table2.2. Quantification of the intensity of the bands was performed using MultiGauge V.3.0 software (Fujifilm). Gapdh mRNA served as an internal control, and expression levels of MLV were set to 1 for the wild-type littermate controls. Results are expressed as mean ± SEM.
Mouse liver tissue (1 g) was homogenized in buffer H (250 mM sucrose, 3 mM CaCl2 in 20 mM Tris pH 7.4) in the presence of a cocktail of protease inhibitors cocktail (Roche Applied Science, Basel, Switzerland) and spun in a centrifuge at 280 g, for 10 min at 4°C (A-4-81 rotor; Eppendorf North America, Westbury, NY, USA). The resulting pellet was collected and washed three times in phosphate-buffered saline (PBS) and carefully resuspended in a 2.4 M sucrose cushion (2.4 M sucrose, 1 mM MgCl2, 10 mM Tris pH 6.8 and protease inhibitor cocktail). The suspension was centrifuged at 168,000 g for 3 hours at 4°C (SW41 Ti rotor; Beckmann-Coulter Inc., Brea, CA, USA). The nuclear fraction was carefully collected and washed three times in PBS plus protease inhibitor cocktail, then counted and divided into aliquots. Following this, 1.5 × 106 nuclei were crosslinked for 10 min in 1% paraformaldehyde at room temperature and sheared by sonication to produce DNA fragments averaging 500 bp in length. The sheared chromatins were immunoprecipitated with anti-macroH2A1 antibody (cat. no. 07-219; Upstate Biotech). Precipitated DNA and protein complexes were uncrosslinked and purified by phenol/choloroform extraction. Purified DNA was used as templates for qPCR quantification using Tbg or Lpl specific primers listed in Table Table22.
The authors declare that they have no competing interests.
MB, SS, RC, RP and PB conceived of and designed the experiments. MB, SS, RC, RP and HD performed the experiments. MB, SS, RC, RP, HD and PB analysed the data. MB and SS generated the H2afy-targeted mouse strain. MB, SS and PB coordinated the work and co-wrote the manuscript. All authors read and approved the final manuscript.
This work was supported by grants from CNRS, ANR (NT05-1_41978), Association pour la Recherche contre le Cancer (ARC n°3901) and Région Rhone-Alpes (MIRA 2008). MB was supported in part by ARC predoctoral fellowship. We thank the Plateau de Biologie Expérimentale de la Souris (ENS, Lyon) for assistance in the generation and the breeding of mutant mice, and the Institut Clinique de la Souris, Strasbourg, D Watrelot-Virieux and C Thirion-Delalande for histological analyses. We are grateful to TH Bestor for helpful comments on the manuscript.