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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Nature. Author manuscript; available in PMC 2010 April 19.
Published in final edited form as:
PMCID: PMC2856441
NIHMSID: NIHMS191408

Innate immunity induced by composition-dependent RIG-I recognition of Hepatitis C virus RNA

Abstract

Innate immune defenses are essential for the control of virus infection and are triggered through host recognition of viral macromolecular motifs known as pathogen-associated molecular patterns (PAMPs) 1. Hepatitis C virus (HCV) is an RNA virus that replicates in the liver, and infects 200 million people 2. Infection is governed by hepatic immune defenses triggered by the cellular RIG-I helicase. RIG-I binds PAMP RNA and signals IRF-3 activation to induce the expression of α/β interferon (IFN) and antiviral/interferon-stimulated genes (ISGs) that limit infection 310. Here we identified the poly-uridine motif of the HCV genome 3’ nontranslated region (NTR) as the PAMP substrate of RIG-I, and show that this and similar homopoly-uridine motifs present in the genome of RNA viruses is the chief feature of RIG-I recognition and immune triggering 8. 5’ terminal triphosphate on the PAMP RNA was necessary but not sufficient for RIG-I binding, which was primarily dependent upon homopolymeric ribonucleotide composition, linear structure and length. The HCV PAMP RNA stimulated RIG-I-dependent signaling to induce a hepatic innate immune response in vivo, and triggered IFN and ISG expression to suppress HCV infection in vitro. These results provide a conceptual advance by identifying homopoly-uridine motfis present in the genome of HCV and other RNA viruses as the PAMP substrate of RIG-I, and define immunogenic features of the PAMP/RIG-I interaction that could be utilized as an immune adjuvant for vaccine and immunotherapy approaches.

To determine the nature of the HCV PAMP RNA we conducted a functional screen to identify possible HCV PAMP RNA motifs. We assessed the ability of full length HCV genome RNA or contiguous HCV RNA segments to trigger the IFN-β promoter in transfected Huh7 cells. The full-length HCV genome RNA triggered innate immune signaling to induce the IFN-β promoter (Fig. 1a). Two regions of the HCV RNA, encoding nt 2406-3256 and nt 8872-9616, stimulated significant induction of the IFN-β promoter (Fig. 1b) with signaling activity respectively localized to nt 2406-2696 of the open reading frame and nt 9389-9619 encoding the 3’ NTR (Fig.1c). Deletion of nt 9389-9619 but not nt 2408-2663 from the HCV genome significantly attenuated signaling to the IFN-β promoter (Fig 1d). PAMP motifs are typically conserved among strains of a pathogen 1, and sequence comparison of multiple HCV genomes revealed global variability within nt 2406-3696 among virus strains but nt 9389-9616 encoded motifs of high conservation (Fig. S1) 11. Thus, the viral 3’ NTR might encode HCV PAMP motifs that trigger innate immune signaling in the host cell.

Figure 1
Identification of HCV PAMP RNA

The HCV 3’ NTR is comprised of three regions: a variable region (VR) with potential secondary structure, a nonstructured poly-U/UC region containing polyuridine with interspersed ribocytidine, and the terminal X region containing three conserved stem-loop structures (Fig. 1e) 12. We evaluated the ability of RNA encoding the HCV 3’ NTR or each of its regions to trigger intracellular signaling. Since HCV RNA replicates through a negative-sense replication intermediate (RI) 2,13, we included analyses of signaling triggered by the RI counterparts of the 3’ NTR and its composite regions. RNA encoding the genomic or RI poly-U/UC region was sufficient to trigger signaling to the IFN-β promoter but neither the variable nor X region genomic and RI RNA induced promoter signaling (Fig. 1f). The HCV 3’ NTR and poly-U/UC but not the X region RNA motif similarly stimulated signaling when introduced into Hela cells (Fig. S3a). Moreover, in Huh7 cells genomic or RI 3’ NTR and poly-U/UC RNA each stimulated the formation of active IRF-3 dimers and expression of ISG56, an IRF-3 target gene 3, but X region RNA failed to trigger either (Fig 1g). The poly-U/UC but not X region RNA formed a stable complex with purified RIG-I (Fig. 1h). These results define the 100 nt poly-U/UC region of the HCV genome and RI RNA as the HCV PAMP motif and potential substrate of RIG-I signaling. We also found that the entire HCV 5’ NTR, which contains four major stem-loop structures comprising the viral internal ribosome entry site 14, was only a weak inducer of promoter signaling. However, prior treatment of cells with IFN-β to increase RIG-I levels 4 rendered responsiveness to signaling triggered by the HCV 5’ NTR or X region RNA (Fig. S3). Thus, dsRNA regions of the HCV RNA are not potent PAMPs but may confer signaling during the IFN response.

To determine the role of RIG-I or other PRR pathways in HCV PAMP signaling, we first examined IFN-β promoter induction in Huh7.5 cells encoding nonfunctional RIG-I 4. The cells were refractory to HCV RNA-induced signaling whereas their response was rescued and enhanced upon overexpression of wild type RIG-I (Fig. 2a). MDA5 is a PRR related to RIG-I that binds to dsRNA 15, whereas MyD88 and TRIF are essential adaptor proteins respectively used by Toll like receptor (TLR) 7/8 and TLR3, which are PRRs that recognizes poly-U RNA, single-stranded RNA or dsRNA 1,16. We examined PAMP signaling in mouse embryonic fibroblasts (MEFs) lacking RIG-I, MDA5, MyD88 or TRIF (Fig S4). When introduced into RIG-I−/− MEFs the HCV RNA failed to trigger promoter activation while 3’ NTR and poly-U/UC but not X region RNA stimulated signaling in wild type, MDA5−/−, MyD88−/− or TRIF−/− cells (Figs. 2b-e). In Huh7 cells, poly-U/UC RNA colocalized and mediated a specific interaction with RIG-I (Fig. 2f). Thus, RIG-I is the essential PRR that signals the innate immune response triggered by HCV poly-U/UC RNA independently of MDA5, MyD88 or TRIF-dependent PRR pathways.

Figure 2
RIG-I-specific HCV RNA PAMP recognition and signaling

RIG-I binds to PAMP RNA containing 5’ppp through which the triphosphate end is proposed to anchor the RNA within charged residues of the RIG-I repressor domain (RD), causing a conformation change to displace the RD and release signaling autorepression 1719. Gel-shift assays revealed that 5’ppp was required for poly-U/UC RNA binding by RIG-I but did not mediate stable RIG-I interaction with X region RNA (Fig. 3a). 5’ppp was required for IFN-β promoter signaling by poly-U/UC RNA, and supported low-level promoter induction triggered by X region RNA in IFN-treated cells (Fig. 3b). Since X region RNA failed to bind RIG-I but weakly triggered signaling, stable RIG-I/RNA interaction is most-likely required to release RIG-I autorepression. We therefore conducted limited trypsin digestion analysis of purified RIG-I alone or bound to poly-U/UC or X region RNA containing 5’ppp. This approach provides an assessment of RIG-I RD displacement in response to PAMP RNA binding wherein the displaced RD of signaling-active RIG-I presents as a protected 17 kDa fragment 1719. As shown in Fig. 3c, RIG-I binding of poly-U/UC but not X region RNA rendered the protected 17 kDa RD fragment. These results indicate that 5’ppp is necessary but not sufficient for RNA binding by RIG-I wherein the HCV poly-U/UC RNA directs stable interaction with RIG-I in a 5’ppp-dependent manner that confers RIG-I signaling activation. We employed 5’ppp RNA in all further experiments.

Figure 3
Poly-uridine and poly-adenosine ribonucleotides are RIG-I ligands

The HCV polyU/UC region is a flexible motif among HCV strains and is essential for viral replication 13. In the HCV genotype 1b strain used in these experiments, the poly-U/UC region is comprised of 100 nt containing 78% uridine and 22% ribocytosine (Fig. S4). We evaluated the requirements of length and nt composition for RIG-I signaling by the poly-U/UC motif. A reduction of length through progressive 3’ truncation to 50 nt or less attenuated signaling in Huh7 cells (Fig. 3d), consistent with reduced RIG-I binding of short RNAs 20. Replacement of ribocytosine with riboguannine (poly-U/UG) had no impact on PAMP signaling but progressive replacement of uridine for riboguanine to below 80% uridine or 100% polyriboguanine (poly-G) reduced or abrogated PAMP signaling, respectively, in both Huh7 cells (Fig. 3e) and in Hela cells (Fig. S2b). Since the RI poly-U/UC RNA contains high polymeric riboadenosine composition, we also examined the impact of poly-A composition and RNA length on RIG-I signaling induced by 100 nt constructs (Fig. S4a). Synthetic poly-A RNA and poly-U/UC RNA equally induced signaling to the IFN-β promoter whereas reduced length to 50 nt or lower of the poly-A RNA significantly attenuated signaling activity (Fig S2c). Moreover, reduced riboadenosine content of the RI poly-U/UC RNA or alteration to poly-G comparatively attenuated or ablated signaling, respectively (Fig. 3f). In side-by-side analyses we found that poly-U/UC, poly-U/UG, and poly-A RNA, but neither poly-C nor poly-G RNA could trigger signaling to the IFN-β promoter (Fig. 3g). Whereas poly C and poly G RNA bound negligibly to RIG-I, poly-U/UC, poly-U/UG and poly-A RNA formed a stable complex with RIG-I (Fig. S4b) and and release of the RIG-I RD to the active conformation (Fig. 3h). These results demonstrate that 50-100 nt length polymeric uridine or riboadenosine motifs, including the PU/UC and RI PU/UC motif of HCV, constitute the PAMP motif that is efficiently recognized by RIG-I to trigger innate immune response.

To determine if RIG-I recognizes the HCV poly-U/UC PAMP RNA to trigger hepatic innate immune defenses in vivo, we conducted RNA signaling analysis in wild type and RIG-I−/− mice. Intravenous administration of full-length HCV 1b genome stimulated hepatic IFN-β mRNA expression within 8 hrs in wild type mice but not in RIG-I−/− mice, and this occurred in a manner dependent on the viral 3’ NTR (Fig. 4a). Moreover, the poly-U/UC RNA but not the X region RNA motif was sufficient to trigger the hepatic IFN-β expression in wild type but not in RIG-I−/− mice. In time course studies we found that the poly-U/UC RNA motif induced a peak of hepatic IFN-β mRNA expression and IFN-β serum levels at 8 hrs post-injection in wild-type mice (Figs. 4b and 4c). This response associated with induced hepatic expression of RIG-I and ISG56 mRNA and tissue-wide expression of hepatic ISG54 (Figs. 4d–4f), similar to the hepatic response in HCV-infected patients 9,10. RIG-I−/− mice expressed only a low level of IFN-β and ISG56. The tissue-wide nature of hepatic ISG54 expression in wild-type mice suggests that paracrine signaling of IFN-β could play an important role in hepatic defenses against HCV. To test this idea we measured HCV production from Huh7 cells that were treated with IFN-β or supernatants collected from cultures transfected with HCV poly-U/UC RNA, X-region RNA, or tRNA (control). Poly-U/UC RNA triggered IFN-β expression in the transfected cells (data not shown), and only treatment with IFN-β or supernatant from the poly-U/UC-transfected cells induced a response that suppressed HCV infection (Figs. 4g and 4h). Thus, the poly-U/UC RNA is an HCV genome PAMP that is necessary and sufficient to trigger RIG-I signaling of the hepatic innate immune response. The actions of RIG-I signaling can induce an antiviral response directly (Fig. 1g) and through indirect, paracrine actions of IFN produced from HCV PAMP signaling (Figs. 4g and 4h).

Figure 4
HCV PAMP RNA triggers the hepatic innate immune response and anti-HCV defenses

Our results provide new insights into the features of RNA PAMP specificity of RIG-I wherein nt composition consisting of viral genome RNA poly-U and respective RI RNA poly-A of greater than 50 nt length is a major determinant that confers RIG-I binding and signaling. 5’ppp was necessary but not sufficient for stable binding of HCV PAMP RNA by RIG-I. In terms of the HCV genome, well-defined internal RNA interactions of the 5’ and 3’ ends 21,22 could provide 5’ppp and PAMP motif proximity for stable RIG-I binding. The poly-U/UC motif is an essential determinant of HCV replication fitness 21. Thus, while the virus must maintain this motif for its viability, the host takes advantage of this requirement and targets the poly-U/UC region as a discriminator of PAMP RNA through RIG-I interaction. Poly-U and/or poly-A motifs are present in localized regions in the genome of RNA viruses known to trigger RIG-I signaling (Table S1) 5,8,19. We found that 5’ppp genomic poly-U/A rich RNA motifs encoded in the rabies virus leader, Ebola virus 3’ region, or the measles virus leader sequence each triggered signaling to the IFN-β promoter in Huh7 cells but GC-rich RNA motifs from each viral genome did not trigger a significant response (Figs. S5a and S5b). We also found that pppT3-63, Tri-GFP and EGFP #2 T7 RNAs, previously described as RIG-I substrates and comprised of 50% or fewer A/U nts 5,18,23 , could induce only weak signaling to the IFN-β promoter compared to the poly-U/UC RNA . Thus, A/U composition and poly-U motifs are major determinants of viral PAMP RNA recognition by RIG-I. Cellular RNAs also contain poly-U and poly-A motifs but mRNAs are typically capped and are bound by poly-A binding proteins 24 while ribosomal RNA are “masked” as ribonucleoprotein complexes 25. These features and the context of 5’ppp with viral poly-U and poly-A motifs serve to identify self from nonself RNA by governing RIG-I recognition, wherein, non-self recognition of the HCV PAMP RNA triggers a hepatic innate immune response. These observations provide a possible explanation of why the 25% or more of all HCV-exposed people clear acute infection 2 and why HCV needs to evade innate immunity through the viral NS3/4A protease targeting of the RIG-I pathway 26,27. RIG-I substrates such as the poly-U/UC RNA or structurally-similar compounds could provide therapeutic application as immune adjuvants similar to TLR agonists 28, and offer innate immune stimulatory properties that may improve IFN-based therapy for HCV through paracrine immune actions that limit infection 2.

Methods Summary

RNA

RNA was synthesized from plasmid DNA, PCR products or annealed DNA oligonucleotides encoding the T7 promoter using the T7 Megascript kit or MEGAshortscript kit (Ambion). Full-length HCV RNA and subgenomic constructs were respectively produced from plasmid DNA or T7 promoter-linked PCR products generated from cloned HCV N (A gift from S. Lemon) or Con1 genome RNA (genotype 1b) 29. Chemically synthesized 5’OH RNAs were purchased from Fidelitysystems. RNA transfection was performed using the Transmessenger kit (QIAGEN). 1 µg of RNA was transfected into 1x105 cells. This mass of RNA is equivalent to the following number of moles: Full-length HCV 1b genome, HCV 1b Δ2408-2663 and HCV 1b Δ3’NTR: 0.4 pmol; HCV 1b subgenomic RNA constructs: 5 or10 pmol, HCV genotype 1b (Con1) 5’NTR, 3’NTR (20 pmol), and all other RNA constructs: 30 pmol. RNA concentrations in the transfection mix were 5-10 µg/ml. RNA expression was determined by RT-quantitative PCR assay. RNA delivery efficiency was assessed as described in Fig S6.

RNA signaling analysis

IFN-β promoter-luciferase analyses of transfected cells were conducted as described 17. Protein expression and the abundance of IRF-3 dimer and monomeric forms in transfected cells were determined by immunoblot analysis of extracts 17.

RIG-I purification and RNA binding analysis

Full-length RIG or RIG-I aa 1-228 (N-RIG) were expressed in E. coli and purified by column chromatography. RIG-I/RNA complexes were assessed by gel-shift assay of purified RIG-I reacted with RNA, separated by agarose gel electrophoresis, and visualized by Sybr Green staining. RIG-I activation/conformation shift was analyzed using the limited trypsin digestion method as described 17. FRET analysis of Cy3-poly-U/UC RNA interaction with YFP-RIG-I or YFP-DAI protein constructs was conducted using N-FRET on a Ziess confocal microscope 30. Serum IFN-β levels were measured by ELISA (PBL, Inc.)

Mice

Wt and RIG-I-/- mice 15 were from Dr. S. Akira. RNA was transfected in mice using the lipid based in vivo RNA transfection reagent (Altogen).

Statistical analysis

Differences between groups were analyzed for statistical significance by the Student's t-test.

Supplementary Material

supplementary data

Acknowledgements

We thank Dr. T. Fujita helpful discussion of our data, and Dr. S. Horner for helpful discussion and manuscript review. We thank Drs. T. Fujita, S. Lemon, G. Sen, C. Rice, T. Taniguchi, A. Miyawaki, S. Akira for reagents, R. Hirai for technical consultations and S. Thomas and G. Martin for technical assistance. Supported by funds from the State of Washington, and National Institutes of Health grants AI060389, AI40035, the Burroughs-Wellcome Fund, and a gift from Mr. and Mrs. R. Batcheldor.

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