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Expanding knowledge on the humoral immune response in Staphylococcus aureus-infected patients is a mandatory step in the development of vaccines and immunotherapies. Here, we present novel insights into the antibody responses following S. aureus bacteremia. Fifteen bacteremic patients were followed extensively from diagnosis onwards (median 29 days, range 9–74). S. aureus strains (median 3, range 1–6) and serial serum samples (median 16, range 6–27) were collected. Strains were genotyped by pulsed-field gel electrophoresis (PFGE) and genes encoding 19 staphylococcal proteins were detected by polymerase chain reaction (PCR). The levels of IgG, IgA, and IgM directed to these proteins were determined using bead-based flow cytometry. All strains isolated from individual patients were PFGE-identical. The genes encoding clumping factor (Clf) A, ClfB, and iron-responsive surface-determinant (Isd) A were detected in all isolates. Antigen-specific IgG levels increased more frequently than IgA or IgM levels. In individual patients, different proteins induced an immune response and the dynamics clearly differed. Anti-ClfB, anti-IsdH, and anti-fibronectin-binding protein A IgG levels increased in 7 of 13 adult patients (p<0.05). The anti-IsdA IgG level increased in 12 patients (initial to peak level: 1.13–10.72 fold; p<0.01). Peak level was reached 7–37 days after diagnosis. In a bacteremic 5-day-old newborn, antistaphylococcal IgG levels declined from diagnosis onwards. In conclusion, each bacteremic patient develops a unique immune response directed to different staphylococcal proteins. Therefore, vaccines should be based on multiple components. IsdA is immunogenic and, therefore, produced in nearly all bacteremic patients. This suggests that IsdA might be a useful component of a multivalent staphylococcal vaccine.
Staphylococcus aureus is a leading cause of nosocomial bloodstream infections . Risk factors for these invasive infections are intravascular catheters and nasal carriage . Nasal carriers have a three- to four-fold increased risk of acquiring a nosocomial bacteremia as compared to non-carriers [3, 4]. Bacteremic patients can develop serious complications, such as infective endocarditis, prosthetic device infection, septic arthritis, deep tissue abscesses, and vertebral osteomyelitis [5–8]. S. aureus bloodstream infections extend the length of hospital stay and increase antibiotic usage, costs, and mortality; approximately 20–30% of the patients die [1, 9, 10]. Worldwide, the increasing resistance of S. aureus isolates to various antibiotics complicates the treatment of bacteremia [11, 12]. Meanwhile, the number of new approved antimicrobial agents has decreased over the last several years . Therefore, alternative strategies to prevent and treat S. aureus bacteremia, such as vaccines and immunotherapy, are urgently required . For the development of these alternative strategies, expanding knowledge on the humoral immune response in S. aureus-infected patients is a mandatory step. In this study, we generate novel insights in the antibody responses following S. aureus bacteremia.
Fifteen patients (13 adults and two children, Table 1) admitted to the Erasmus MC, Rotterdam, The Netherlands, from March to June 2008 were followed from the diagnosis of S. aureus bacteremia until the end of hospital stay (range 9–74 days, median 29 days). Bacteremia was defined upon isolation of S. aureus from at least one blood culture set. From these patients, a total of 44 methicillin-susceptible S. aureus strains were collected for routine culture. The median number of collected strains per patient was 3 (range 1–6). The S. aureus strains were isolated from blood, sites of infection, and, if applicable, other sites. Furthermore, serial serum samples were collected (leftover material). The median number of days between the first positive blood culture and the first sampling of serum was 1 day (range 0–20 days). From each patient, at least 6 (median 16, range 6–27) serum samples were collected, leading to a total of 232 serum samples. The time between the first positive blood culture and the first negative (control) blood culture ranged from 1–6 days (median 2 days; excluding one patient for whom no additional blood culture was obtained).
Sera from four non-S. aureus bacteremic patients were used as controls. These four patients were diagnosed with a Klebsiella pneumoniae, Proteus mirabilis, coagulase-negative staphylococcus (CNS), or Enterococcus faecium bacteremia. The number of days between the first positive blood culture and the first sample ranged from 0 to 2 days. Per patient, 12–26 (median 16) serum samples were collected. The follow-up time ranged from 22 to 50 days (median 29 days).
Patients were treated with antibiotics according to hospital guidelines under the supervision of Infectious Disease consultants. Catheters were removed if they were the suspected origin of the bacteremia. The Medical Ethics Committee of the Erasmus MC, Rotterdam, The Netherlands, approved the study (MEC 2007-106, addendum 2).
S. aureus was identified based on colony and microscopic morphology and Slidex Staph Plus agglutination testing (bioMérieux). The identification of S. aureus was confirmed by spa polymerase chain reaction (PCR) . The isolates were screened for genes encoding important staphylococcal proteins: the microbial surface components recognizing adhesive matrix molecules (MSCRAMMs), staphylococcal enterotoxins (SEs), and immunomodulatory proteins [16–20]. Sequences specific for clumping factor A and B (ClfA and ClfB), S. aureus surface protein G (SasG), iron-responsive surface determinant A and H (IsdA and IsdH), fibronectin-binding protein A and B (FnbpA and FnbpB), serine-aspartate dipeptide repeat protein D and E (SdrD and SdrE), SEA, SEB, SEI, SEM, SEO, SEQ, toxic shock syndrome toxin (TSST-1), staphylococcal complement inhibitor (SCIN), extracellular fibrinogen-binding protein (Efb), and chemotaxis inhibitory protein of S. aureus (CHIPS) were detected. Primers for isdA (Fw, CTGCGTCAGCTAATGTAGGA; Rv, TGGCTCTTCAGAGAAGTCAC), isdH (Fw, TCTGCTGGTGGATACTGTTG; Rv, TGCCAGTGAGACTTGTATCG), sasG (Fw, GGCCACTTGGATGAGTTGGT; Rv, CGAAGAGCCAGTGGATGATG), sdrD (Fw, CGGAGCTGGTCAAGAAGTAT; Rv, TGCCATCTGCGTCTGTTGTA), and efb (Fw, GAAGGATACGGTCCAAGAGA; Rv, TGTGGACGTGCACCATATTC) were newly designed. Other genes were detected by PCR as described previously [21–26]. Furthermore, S. aureus strains were genotyped by pulsed-field gel electrophoresis (PFGE) .
The levels of IgG, IgA, and IgM in the serum of the patients directed against the MSCRAMMs, SEs, and immunomodulatory proteins were quantified using a bead-based flow cytometry technique (xMAP, Luminex Corporation). This technique allows for the quantification of antibodies in small serum volumes to 19 antigens simultaneously. For IgG and IgA measurement, sera were diluted 1:100. For IgM measurement, sera were diluted 1:25. The methods were as described previously [16, 28, 29]. Tests were performed in independent duplicates and the median fluorescence intensity (MFI) values, reflecting antibody levels, were averaged. In each experiment, control beads (no protein coupled) were included to determine non-specific antibody binding. In case of non-specific binding, these non-specific MFI values were subtracted from the antigen-specific results. Human pooled serum (HPS) was used as a standard.
Statistical analyses were performed with SPSS version 15.0. To compare the initial antibody level with the peak antibody level, the Wilcoxon matched-pairs signed-ranks test was used. p<0.05 was considered as statistically significant.
PFGE analysis was performed for all S. aureus strains isolated. A dendogram of the PFGE data (Fig. 1) shows the overall lack of relatedness among the strains from different patients, with the exception of patients 4 and 5. There was no epidemiological relationship between these two patients. All S. aureus strains isolated from an individual patient were genotypically indiscriminate (>95% relatedness) and clustered in the dendogram; only in the case of patient 9 were the PFGE patterns of the strains slightly different.
Based on PCR analyses, clfA, clfB, and isdA were ubiquitous in all 44 isolates obtained from the 15 bacteremic patients. scn, efb, fnbA, and isdH were detected in >90% of the isolates. sdrD, sdrE, sasG, sei, seo, sem, chp, and fnbB were detected in 35 (80%), 33 (75%), 30 (68%), 28 (64%), 28 (64%), 23 (52%), 23 (52%), and 14 (32%) of the isolates, respectively. Less than 20% of the isolates harbored sea, seb, seq, and tst-1. Overall, all strains isolated from a single patient (PFGE-identical) harbored the same virulence genes, with two exceptions. In patient 6, scn was detected only in the strain isolated from foot tissue, but not in the strains isolated from blood and the superficially cultured wound of the foot. In patient 15, sasG was detected only in the strain isolated from catheter-drawn blood, but not in the strain isolated from peripheral blood or bone marrow (Fig. 1). An overview of the presence of virulence genes in S. aureus strains per patient is shown in Table 2.
The levels of IgG, IgA, and IgM directed to 19 S. aureus proteins were measured from diagnosis bacteremia onwards. In Fig. 2, the changes in antistaphylococcal IgG, IgA, and IgM levels after diagnosis bacteremia are shown for one patient (patient 4). For all patients, the course of the antibody response was determined. If there was an increase in antibody level in the consecutive serum samples, the fold increase from initial antibody level (as measured in the first obtained serum sample) to peak level was calculated. An overview for the 13 adult patients is shown in Table 2. In each patient, increases in antistaphylococcal antibody levels directed to different S. aureus proteins were observed. Antibody responses were directed mainly to the immunomodulatory proteins and MSCRAMMs. An increase in anti-IsdA IgG level was detectable in 12 out of 13 (92%) adult bacteremic patients (Fig. 3; with the exception of patient 12). Some patients showed a small decline in anti-IsdA IgG level before the increase. The median increase from initial to peak level was 1.71-fold (range 1.13–10.70-fold, p<0.01). The number of days to reach anti-IsdA IgG peak levels ranged between 7 and 37 days (median 21 days). It appeared that patients with a site of infection other than catheter-related (patients 6, 7, 8, 11, and 13) reached higher levels, but the anti-IsdA IgG peak level was high in patient 2 as well (Fig. 3). In Table 3, the dynamics of the IgG responses directed against all immunomodulatory proteins and MSCRAMMs are summarized per S. aureus protein.
An increase in antigen-specific IgA was detected less frequently than an increase in IgG. In 7 out of 13 patients with a gene-positive isolate, the anti-IsdA, anti-SCIN, and anti-Efb IgA level increased. Peak levels were reached between 6 and 34 days. In not a single patient was a rise in anti-FnbpB, SasG, SEA, SEB, SEO, or SEQ IgA level detected. Increases in IgM were detected the least often out of the three antibody isotypes (Table 2). For patients 9 and 11, most antigen-specific IgM levels showed a decrease.
In the two children, the antibody responses were quite different from the antibody responses in adults. In the 5-day-old newborn (patient 14), a continuous decline in antigen-specific (maternal) IgG for 18 out of 19 proteins was seen from the moment of diagnosis. This could be due to the extensive consumption of maternal antibodies in the absence of de novo antibody synthesis (Fig. 4). The level of IgM increased for CHIPS (isolate chp-negative), SCIN, SasG, SdrD, SdrE, SEM, SEO, and SEQ (seq-negative). The level of IgA was around zero. In the 2-year-old child (patient 15), an increase was noted for IgG directed to IsdA and CHIPS only. IgM increased for SCIN, ClfA, SdrD, SdrE (isolate sdrD- and sdrE-negative), SEI, SEM, SEO, and TSST-1 (tst-negative).
In the four control patients who did not suffer from an S. aureus bacteremia, the CNS-infected patient showed a 1.2-fold increase in anti-CHIPS IgG level (from MFI 13333 to MFI 16002), and the K. pneumoniae-infected patient showed a 1.4-fold increase in anti-ClfB IgG level (from MFI 5647 to MFI 8058). Furthermore, no increases in antistaphylococcal antibody levels were noted. This shows that there is little inter-species cross-reactivity. However, in the patients suffering from an S. aureus bacteremia, a rise in antibody level while the corresponding gene was not present in the S. aureus isolate was observed in 23 of 179 increases (12.8%; Table 2).
By using multiple, longitudinally collected serum samples, we were able to show that each bacteremic patient develops a unique S. aureus-specific immune response after infection. In each bacteremic patient, the S. aureus proteins to which the antibody response was directed differed and the height and number of days to reach peak antibody level differed. Therefore, it seems unlikely that a vaccine based on a single staphylococcal component will be effective. There are several possible explanations for the variability of the antistaphylococcal immune response in bacteremic patients. First, it may be the result of the genetic diversity of the S. aureus strains which caused the bacteremia . Secondly, the variability might be due to different staphylococcal protein expression and/or selective protein recognition by the immune system in different patients. By way of illustration, although patients 4 and 5 were infected with PFGE-identical S. aureus strains with similar virulence genes, the antibody responses were very different (Fig. 1 and Table 2). The observed variability in antibody response might also be caused by the difference in the time of onset of the bacteremia, which is difficult to determine accurately for patients with an origin of bacteremia other than catheter-related. Finally, colonization, the number and severity of preceding infections, the level of immunity, and the ability to mount an immune response probably also contribute to the development of the antistaphylococcal humoral immune response after bacteremia .
Although the antistaphylococcal immune response after infection is a unique personal characteristic, IsdA is immunogenic and, therefore, produced by S. aureus in nearly all bacteremic patients. IsdA interacts with and binds to fibrinogen and fibronectin of human cells , and is involved in the adherence of S. aureus to human desquamated nasal epithelial cells . Furthermore, IsdA is required for nasal colonization in the cotton rat model . Antibodies reactive to IsdA enhanced the killing of S. aureus by the antimicrobial peptide apolactoferrin in vitro . Since vaccines should contain multiple components that are expressed in vivo in many, if not all, patients, it seems that IsdA is an interesting target for inclusion in a multicomponent vaccine .
In 23 of 179 (12.8%) observed increases, a rise in the antistaphylococcal antibody level was found while the corresponding gene was not detected in the S. aureus isolate. Increasing anti-TSST-1, anti-SEB, and anti-FnbpB antibody levels in the absence of tst, seb, and fnbB genes were documented most frequently (Table 2). This might be the result of immunological cross-reactivity. In earlier studies, it was shown that anti-SEB cross-reacts with anti-SEC1 [36, 37]. Furthermore, it was shown that anti-TSST-1 also has cross-inhibitory activity against SEA and SEB [38, 39]. However, the relative binding affinity was 1,000-fold lower for SEA than for TSST-1 and, therefore, this does not seem to be clinically relevant. Another explanation might be that the antibodies are secreted by plasma cells that are mobilized from their survival niche in the bone marrow by competition with newly generated plasma blasts . Knowledge on non-specific antibody binding should be increased, however.
In summary, bacteremic patients usually carry a PFGE-identical S. aureus strain at multiple sites. Each bacteremic patient develops a unique immune response directed against different S. aureus proteins. Therefore, a vaccine based on a single antigenic component is not likely to be effective. The surface protein IsdA is immunogenic and, therefore, produced by S. aureus in nearly all bacteremic patients. This suggests that IsdA might be a useful component of a multivalent staphylococcal vaccine.
We thank our colleagues from the Department of Clinical Chemistry, Erasmus MC, Rotterdam, The Netherlands, for their help with collecting the serum samples. We thank Prof. Dr. Barbara Bröker and Dr. Silva Holtfreter for their useful comments.
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