bazX-82 affects germ plasm localization during oogenesis. Confocal images of wild-type (WT) (A,C,E,G,I) and bazX-82 (B,D,F,H,J) egg chambers. (A-D) FISH to osk mRNA in stage 10 oocytes. (E-J) Direct visualization of Vas-GFP (E,F) in stage 10 oocytes (arrow in F) and Osk-GFP (G,H) and Vas-GFP (I,J) in stage 14 oocytes. Germ plasm mRNA (osk) and protein (Osk, Vas) components are ectopically localized in bazX-82 oocytes. (K,L) Confocal images of egg chambers expressing Osk-GFP. (K) Wild-type egg chamber. (L) A rare baz4 egg chamber where an oocyte is specified. Nuclei are labeled with WGA.