VEGF neuroprotection against G93A-SOD1 in cultured primary MNs
(A) TUNEL labeling demonstrates increased DNA damage associated with 500 and 1000 pfu/MN AdmSOD transfection, which is significantly attenuated in the presence of VEGF (250 ng/ml). Transfection with equal concentrations of AdwtSOD1 does not result in increased DNA damage. MN densities are equivalent between treatments groups and results are averaged over at least 10 fields of 3 independent experiments and analyzed for statistical significance using one-way ANOVA followed by Tukey’s multiple comparison test (*P < 0.05). (B) Western blotting demonstrates decreased levels of G93A-SOD1-induced caspase-3 cleavage with VEGF treatment. Pretreatment (4 h pre) is the most efficacious, with decreased efficacy associated with VEGF cotreatment or posttreatment (18 h post).