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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Dev Neurobiol. Author manuscript; available in PMC 2010 November 1.
Published in final edited form as:
Dev Neurobiol. 2009 November; 69(13): 871–884.
doi: 10.1002/dneu.20747

Figure 3

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VEGF signaling in cultured primary MNs

Western blotting of primary MNs treated with VEGF. (A) Time course of Akt and p44/42 MAPK phosphorylation in response to VEGF (250 ng/ml) treatment. (B) Pretreatment (1 h) with inhibitors to the PI3K/Akt pathway (LY294002; 20µM), p44/42 MAPK pathway (U0126; 20µM), and VEGFR2 (SU1948; 20µM) prevent activation of the respective signaling pathways in response to VEGF treatment.

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