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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
N Engl J Med. Author manuscript; available in PMC 2010 April 8.
Published in final edited form as:
PMCID: PMC2851618
NIHMSID: NIHMS185272

STIM1 Mutation Associated with a Syndrome of Immunodeficiency and Autoimmunity

SUMMARY

A mutation in ORAI1, the gene encoding the pore-forming subunit of the Ca2+-release–activated Ca2+ (CRAC) channel, abrogates the store-operated entry of Ca2+ into cells and impairs lymphocyte activation. Stromal interaction molecule 1 (STIM1) in the endoplasmic reticulum activates ORAI1–CRAC channels. We report on three siblings from one kindred with a clinical syndrome of immunodeficiency, hepatosplenomegaly, autoimmune hemolytic anemia, thrombocytopenia, muscular hypotonia, and defective enamel dentition. Two of these patients have a homozygous nonsense mutation in STIM1 that abrogates expression of STIM1 and Ca2+ influx.

Combined Immunodeficiency Disease is a Rare Familial Disorder that can be caused by mutations in a variety of genes.1,2 A principal feature of most cases of combined immunodeficiency disease is impaired function of T, B, or natural killer cells, despite their normal development. A defect in Ca2+-dependent signaling in lymphocytes has been found in some types of immunodeficiency,1,2 and here, we describe a newly discovered mutation that impairs lymphocyte activation in patients with combined immunodeficiency disease by disrupting Ca2+ entry into lymphocytes.

The release of Ca2+ from intracellular stores facilitates the influx of Ca2+ from the extracellular compartment, which is essential for activation of lymphocytes, by means of store-operated Ca2+-entry channels. In T cells, these channels are CRAC channels.35 The electrophysiological characteristics of CRAC channels include high Ca2+ selectivity and a gating mechanism that depends on the depletion of Ca2+ stores in the endoplasmic reticulum. Two molecules, STIM1 and ORAI1, mediate the function of CRAC channels and the store-operated entry of Ca2+ through them.610 STIM1 senses the Ca2+ concentration in the endoplasmic reticulum and activates CRAC channels in the plasma membrane, and ORAI1 is the pore-forming subunit of the CRAC channel.1113 Our group reported previously that a missense mutation in ORAI1, resulting in a loss of CRAC-channel function and store-operated Ca2+ entry, causes a severe defect in T-cell activation and underlies combined immunodeficiency disease in two patients from one kindred.8,14 Here, we describe three patients from one kindred with immunodeficiency, autoimmune hemolytic anemia, thrombocytopenia, muscular hypotonia, and disturbed enamel dentition. Two of these siblings, whose DNA was available for study, were homozygous for a recessive nonsense mutation in STIM1 exon 3. The mutation results in a premature termination codon and consequent truncation of the STIM1 protein, which in turn leads to undetectable expression of the predicted STIM1 protein and defective store-operated entry of Ca2+.

CASE REPORTS

Patient V-1 was born at term to consanguineous parents from central Europe (Fig. 1A). Both parents and five sisters are healthy. Despite normal early cognitive development, the neonatal period of Patient V-1 was conspicuous because of partial iris hypoplasia and nonprogressive global muscular hypotonia (Table 1). Muscle biopsy and electromyography revealed no abnormalities. Lymphadenopathy and hepatosplenomegaly were apparent at 7 months of age; at 15 months she was found to have autoimmune hemolytic anemia, and at 5 years thrombocytopenia, both of which were treated with glucocorticoids. During the first 4 years of life, she had multiple infections caused by a spectrum of pathogens, including cytomegalovirus at 1 month of age, recurrent urinary tract infection, recurrent bacterial sepsis (caused by Streptococcus pneumoniae and Escherichia coli), and two episodes of chickenpox. After the age of 5 years, she had several episodes of otitis media and pneumonia. She also had a defect in enamel dentition, which became apparent in the first years of life. The patient died from complications of hematopoietic stem-cell transplantation, at 9 years of age.

Figure 1
Nonsense Mutation in the Stromal Interaction Molecule 1 Gene (STIM1) and Lack of Store-Operated Ca2+ Entry into Cells
Table 1
Characteristics of Patients with STIM1 Deficiency.*

Patient V-4 was the younger sister of Patient V-1. She had a clinical picture similar to that of her older sister, including severe autoimmune hemolytic anemia, thrombocytopenia, lymphadenopathy, hepatosplenomegaly, partial iris hypoplasia, and muscular hypotonia. She died from severe nephrotic syndrome that was resistant to therapy, encephalitis, and enterovirus infection, at the age of 18 months.

Patient V-7 is the brother of Patients V-1 and V-4. He had three undocumented episodes of sepsis during the first 2 months of life and was treated with intravenous immune globulin since birth. At 1 month of age, he had an episode of thrombocytopenia and at 2 years of age was found to have hypoglycemia. Patient V-7 survived after hematopoietic stem-cell transplantation (with a healthy, HLA-identical sister as donor) performed at 15 months of age, and intravenous immune globulin was stopped. He no longer has an increased susceptibility to infection. Currently, at 6 years of age, he has nonprogressive muscular hypotonia and partial iris hypoplasia only.

METHODS

Written informed consent was obtained from the parents of the patients. The experiments were conducted after approval was given by the institutional review boards at New York University Langone Medical Center and Necker–Enfants Malades Hospital.

Cell Lines And Plasmids

Fibroblasts from Patient V-1 and from controls were immortalized by means of retroviral transduction with human telomerase reverse transcriptase (hTERT), as previously described.8 Cells were transduced with bicistronic retroviral expression vectors containing myc-epitope–tagged STIM1, STIM2, ORAI1, and internal ribosome entry site green fluorescent protein (GFP), as previously described.8 Protein expression was assessed by measuring the expression of GFP and immunoblotting for STIM1 with the use of peptide-specific antibodies.

Genomic Sequencing

Genomic DNA from fibroblasts of Patient V-1 and from control fibroblast cell lines was isolated according to standard methods. For the polymerase-chain-reaction (PCR) assay, oligonucleotide primers flanking the exons of STIM1 and STIM2 encompassing splice sites were used; primer sequences are listed in Table 1 in the Supplementary Appendix (available with the full text of this article at NEJM.org). PCR products were amplified with the use of high-fidelity KOD DNA polymerase (EMD Biosciences), purified with the QIAquick gel extraction kit (Qiagen), and sent directly for sequencing (Genewiz). PCR and sequencing reactions were repeated three times from at least two independent genomic DNA preparations, if mutations were found. Sequence alignments were performed with the use of T-Coffee software (Swiss Institute of Bioinformatics); DNA-sequence traces were visualized with Xplorer software (version 1.0, dnaTools). Singlenucleotide polymorphism (SNP) searches were performed in the National Council for Biotechnology Information’s dbSNP database (build 129; www.ncbi.nlm.nih.gov/SNP). Genomic DNA obtained from 50 white, healthy controls (representing a total of 100 chromosomes) were also sequenced.

Real-time PCR was done as previously described.14 Primers used for the amplification of complementary DNA are listed in the Supplementary Appendix.

Immunoblotting And Antibodies

Flow-cytometric analysis of lymphocytes, assays for the proliferation of peripheral-blood mononuclear cells, and the apoptosis assay were performed as previously described.17 Serum immunoglobulin levels were determined by nephelometry. Immunoblotting was performed on the basis of standard protocols.14 Antibodies against actin (sc-1616, Santa Cruz Biotechnology) and STIM1 (GOK, BD Biosciences) were purchased. A polyclonal antibody against the C-terminal domain of STIM1 was generated by immunizing rabbits with a conserved 29–amino acid peptide corresponding to amino acids 657 to 685 of human STIM1 (NP_003147), according to standard protocols (Open Biosystems). Antisera were affinity-purified with the immunizing peptide. Single-cell measurements of intracellular Ca2+ concentrations were obtained, as previously described.8 Further details on experimental procedures are available in the Methods section of the Supplementary Appendix.

RESULTS

Immunologic Assessments

Lymphocyte counts for Patients V-1, V-4, and V-7 were slightly reduced or normal, with an age-appropriate distribution of lymphocyte subpopulations. Decreased proportions of naive CD4+ T cells and CD4+CD45RA+CD31+ T cells (recent emigrants from the thymus) were found for Patient V-1. Her T-cell repertoire was normal, as evaluated with the use of monoclonal antibodies against T-cell–receptor β-chain–variable regions (Vβ3, Vβ8, Vβ13-6, Vβ13–17, and Vβ21-3). The numbers of CD4+FOXP3+ regulatory T cells were greatly reduced in blood specimens from Patient V-1 (Table 1, and Fig. 1 in the Supplementary Appendix). T-cell proliferation in response to phytohemagglutinin, phorbol 12-myristate 13-acetate plus ionomycin, or an anti-CD3 antibody was moderately to markedly reduced in Patients V-1 and V-7, and these two patients had severely impaired T-cell responses to recall antigens (varicella–zoster virus and tetanus toxoid), despite two episodes of chickenpox (in Patient V-1) and one round of vaccination against diphtheria, pertussis, and tetanus 1 month before testing of the response to recall antigens (in Patient V-7). T-cell apoptosis induced by Fas was normal in Patient V-1. Serum levels of IgG, IgA, and IgM were within or close to the normal range in Patients V-1 and V-7, whereas IgG levels were greatly reduced in Patient V-4, most likely secondary to the nephrotic syndrome.

Nonsense Mutation In Stim1

The stimulation of fibroblasts by thapsigargin, an inhibitor of the sarcoplasmic–endoplasmic reticulum Ca2+ ATPase, revealed a pronounced defect in store-operated Ca2+ entry in Patient V-1 as compared with controls (Fig. 1B). Normally, depletion of intracellular Ca2+ stores by thapsigargin activates store-operated Ca2+ entry through CRAC channels. The defect in this mechanism in fibroblasts of Patient V-1 resembles the defective store-operated Ca2+ entry in ORAI1 deficiency, in which CRAC channels are not functional.8,14 However, genomic DNA sequencing failed to reveal mutations in ORAI1 or its paralogues ORAI2 and ORAI3 in Patient V-1.

Biochemical analysis has shown that STIM proteins are required for the activation of ORAI channels,18,19 and both RNA-interference experiments and transgenic mouse models show that STIM1 is essential for store-operated Ca2+ entry in many types of cells.6,7,2023 The sequencing of genomic DNA obtained from Patient V-1 for STIM1 mutations revealed a 380_381insA mutation, resulting in the insertion of an adenine in exon 3 of STIM1 between positions 380 and 381 in the coding sequence (Fig. 1C). This mutation causes a frame shift that alters eight amino acids (RSIQLDRG) and results in a premature termination codon in STIM1. The same mutation was found in Patient V-7. The 380_381insA mutation is not a known SNP (according to dbSNP, build 129) and was not found in 50 unrelated controls (data not shown). The predicted mutant protein, designated STIM1-E136X, is expected to be truncated at the beginning of a sterile alpha-motif (SAM) domain in the endoplasmic reticulum– luminal portion of STIM1 (Fig. 1D).

Loss Of Stim1

Because premature termination codons often result in nonsense-mediated decay of messenger RNA (mRNA),24 we analyzed mRNA transcript and protein levels for STIM1 in Patient V-1. We found significantly reduced STIM1 mRNA levels in fibroblasts from Patient V-1 as compared with control fibroblasts (P = 0.04), as measured by realtime reverse-transcriptase PCR (Fig. 2A). Transcript levels for STIM2, by contrast, were similar in fibroblasts from Patient V-1 and one control cell line. Expression of the full-length STIM1 protein was not detectable in fibroblasts from Patient V-1, as compared with cells from a control and a previously described patient with ORAI1 deficiency8 (Fig. 2B). An antibody against the N-terminal end of STIM1 also failed to detect full-length STIM1 or the predicted mutant STIM1 fragment (approximately 15 kD in size) in cells from Patient V-1 (Fig. 3 in the Supplementary Appendix). Taken together, these data indicate that our patient’s cells lack or have considerably reduced STIM1 mRNA and protein.

Figure 2
Absence of Functional Stromal Interaction Molecule 1 (STIM1) in Fibroblasts and Restoration of Ca2+ Influx with Addition of Exogenous STIM1 and STIM2

To confirm the causative role of the nonsense STIM1 mutation in defective store-operated Ca2+ entry, we transduced fibroblasts from Patient V-1 with wild-type STIM1, STIM2, ORAI1, or an empty vector using retroviral expression vectors that allow for bicistronic expression of GFP from an internal ribosome entry site. For analysis of Ca2+ influx, GFP-labeled cells were selected. Transduction with ORAI1 failed to rescue the defect in the patient’s fibroblasts, but expression of STIM1 rescued store-operated Ca2+ influx. A similar, although less robust, rescue of store-operated Ca2+ entry was seen after we transduced the patient’s fibroblasts with wild-type STIM2 (Fig. 2C and 2D). A similar effect of STIM2 has been found in Stim1-deficient fibroblasts harvested from mouse embryos.21 Nevertheless, the amount of endogenous STIM2 in the cells from Patient V-1 does not seem sufficient to compensate for the lack of STIM1.

DISCUSSION

One of the strongest indications that Ca2+ influx through CRAC channels is essential for lymphocyte activation and immune responses comes from the study of rare inherited immunodeficiency syndromes involving defects in store-operated Ca2+ entry.8,14,2527 We describe three siblings with a complex immunodeficiency disorder, and detailed studies of one of them revealed reduced or absent STIM1 resulting from a nonsense mutation in STIM1. Whether Patient V-4 has the same mutation in STIM1 is yet unknown but is plausible, because the clinical pictures for all three children were virtually the same.

STIM1 is required to activate store-operated Ca2+ entry in a variety of cells, including lymphocytes.6,7,2023 We found that fibroblasts from a patient with STIM1 deficiency have a severe defect in Ca2+ entry that was rescued by reintroducing wild-type STIM1 into the patient’s cells. The clinical phenotype of the three patients with STIM1 deficiency is similar to that observed in two patients with a missense mutation in ORAI1 (R91W), the pore-forming subunit of the CRAC channel. This mutation abrogates CRAC-channel activity and store-operated Ca2+ entry.8 Patients with deficiencies in both STIM1 and ORAI1 have a primary immunodeficiency marked by susceptibility to viral and bacterial infections and also nonprogressive myopathy and defects in the formation of dental enamel. This overlap of clinical findings suggests that the phenotype resulting from mutations in STIM1 and ORAI1 is not genespecific but, rather, pathway-specific — that is, it reflects the lack of function of CRAC channels.

Of the three siblings we studied, two had autoimmune hemolytic anemia and immune thrombocytopenia; the third, a boy, had only one episode of thrombocytopenia. Other clinical features were hepatosplenomegaly, lymphadenopathy, and, in the boy, hypoglycemia. In mice in which both Stim1 and Stim2 had been deleted in T cells,21 the phenotype consisted of splenomegaly, lymphadenopathy, organ infiltration by leukocytes, dermatitis, and blepharitis. This phenotype is attributed to a defect in the development and function of regulatory T cells caused by the defect in store-operated Ca2+ entry and depressed function of the nuclear factor of activated T cells (NFAT) protein.21 It has been proposed that formation of a complex between the FOXP3 and NFAT transcription factors is required for the development of regulatory T cells.28 Very low numbers of CD4+FOXP3+ T cells were detected in the blood specimens obtained from Patient V-1 (the only patient for whom blood specimens were available), and it is therefore possible that store-operated Ca2+ entry is required for differentiation of regulatory T cells in humans. The three siblings we describe did not have clinical features of the immune dysregulation, polyendocrinopathy, enteropathy, X-linked syndrome,29 which is due to FOXP3 deficiency.

The nonprogressive muscular hypotonia seen in all three siblings correlates with a similar phenotype in patients with ORAI1 deficiency8 and with the defect in muscle development and function in Stim1-deficient mice.20 Muscle dysfunction is severe in such mice, and is probably responsible, at least in part, for the early death seen in Stim1- and Orai1-deficient mice.2023,30

In summary, the clinical phenotype of the three siblings we studied, two of whom had a STIM1 deficiency due to a homozygous nonsense mutation in the STIM1 gene, is remarkably similar to the phenotype of patients with ORAI1 deficiency. The lack of store-operated Ca2+ entry due to defects in ORAI1 or STIM1 interferes with immune function, causing immunodeficiency, autoimmune disease, and myopathy.

Supplementary Material

Supplementary data

Acknowledgments

Supported by grants from the National Institutes of Health (NIH) (to Dr. Rao), from the March of Dimes Foundation and NIH (to Dr. Feske), and INSERM (to Drs. Rieux-Laucat and Fischer).

Drs. Rao and Feske report being scientific cofounders of Calci-Medica, a biotechnology company that seeks to develop CRAC channel inhibitors. No other potential conflict of interest relevant to this article was reported.

We thank Dr. Patrick G. Hogan for critical reading of a previous version of the manuscript; Corinne Jacques, Chantal Harre, Stephanie Ndaga, and Françoise Selz for their technical help; and Marie-Claude Stolzenberg for the immunophenotyping of regulatory T cells. We also thank Dr. Rochelle Hirschhorn (New York University Langone Medical Center) for providing DNA samples obtained from healthy controls and Silvia Menendez and Dr. Eva Hernando (both at New York University Langone Medical Center) for providing the control fibroblast cell lines.

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