PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
 
J Bacteriol. May 1969; 98(2): 536–542.
PMCID: PMC284850
Relation of the Segregative Origin of Chromosome Replication to the Origin of Replication After Amino Acid Starvation
Helen Eberle1 and Karl G. Lark
aDivision of Biology, Kansas State University, Manhattan, Kansas 66502
1 Present address: Department of Radio-Biology and Biophysics, School of Medicine and Dentistry, The University of Rochester, Rochester, N.Y. 14620.
Abstract
Cultures of Escherichia coli 15T and K-12 were labeled with 3H-thymine before, during, and after amino acid starvation. The number of labeled segregating units was measured by autoradiography of microcolonies derived from the labeled cells. In both strains, labels inserted before starvation and during starvation appeared to segregate as if incorporated into the same polynucleotide strands. However, labels inserted during and after starvation segregated as if incorporated into different polynucleotide strands. In view of previous data, it was concluded that replication after amino acid starvation originates from the region of the chromosome which serves as the origin for replication during normal growth and division.
Full text
Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.1M), or click on a page image below to browse page by page.
Images in this article
Click on the image to see a larger version.
Articles from Journal of Bacteriology are provided here courtesy of
American Society for Microbiology (ASM)