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The Boston Puerto Rican Health Study is an ongoing longitudinal cohort study designed to examine the role of psychosocial stress on presence and development of allostatic load and health outcomes in Puerto Ricans, and potential modification by nutritional status, genetic variation, and social support.
Self-identified Puerto Ricans, aged 45-75 years and residing in the Boston, MA metro area, were recruited through door-to-door enumeration and community approaches. Participants completed a comprehensive set of questionnaires and tests. Blood, urine and salivary samples were extracted for biomarker and genetic analysis. Measurements are repeated at a two-year follow-up.
A total of 1500 eligible participants completed baseline measurements, with nearly 80% two-year follow-up retention. The majority of the cohort is female (70%), and many have less than 8th grade education (48%), and fall below the poverty level (59%). Baseline prevalence of health conditions is high for this age range: considerable physical (26%) and cognitive (7%) impairment, obesity (57%), type 2 diabetes (40%), hypertension (69%), arthritis (50%) and depressive symptomatology (60%).
The enrollment of minority groups presents unique challenges. This report highlights approaches to working with difficult to reach populations, and describes some of the health issues and needs of Puerto Rican older adults. These results may inform future studies and interventions aiming to improve the health of this and similar communities.
Racial and ethnic disparities in prevalence and incidence of chronic conditions are an important problem in the United States (US). Their investigation can contribute to progress toward their elimination and improved understanding of the etiology of diseases [1-5]. Reduction of existing racial and ethnic health disparities is a primary concern for ethical and economic reasons. Without improvements, as minority populations grow and age so will the burden on the health care system. Hispanics currently represent 14.8% of the US population and are projected to increase to almost 25% by the year 2050 .
Most epidemiologic research on Hispanics has focused on Mexican Americans, due to their majority as a subgroup. However, evidence suggests that health outcomes vary significantly by Hispanic ethnic subgroup; geographical and ethnic variation have been reported for the prevalence of type 2 diabetes among elderly participants in the US, South America and the Caribbean . There is evidence of variation in management of diabetes among Latino subgroups, which suggests that grouping Hispanics into one category may obscure important differences .
Puerto Ricans are the second largest Hispanic subgroup in the US . They report the worst health status and highest prevalence of several acute and chronic medical conditions, when compared with non-Hispanic whites (NHW) and other Hispanic subgroups . National data show that 21% of older Puerto Ricans reported having an activity limitation, compared with 15% of Cuban and Mexican Americans . Puerto Rican elders living in Massachusetts show significantly greater prevalence of physical disability, type 2 diabetes, depression and other chronic conditions than NHW living in the same neighborhoods [12-18].
There is a paucity of knowledge regarding the health status and behaviors of Puerto Rican adults living in the US. Moreover, little is known about strategies for recruitment and retention when conducting research with this population. Therefore, the goal for this report is to describe the challenges and opportunities for recruitment and assessment of a cohort of Puerto Rican adults living in the Boston, MA area, as well as their baseline characteristics. The Boston Puerto Rican Health Study (BPRHS) is funded as part of the Centers for Population Health and Health Disparities, a special initiative of the National Institutes of Health to foster trans-disciplinary and multilevel research to improve understanding of health disparities in the US .
The BPRHS is an ongoing longitudinal study that aims to examine the role of psychosocial stress on the presence and development of allostatic load (physiological dysregulation) and health outcomes such as depressive symptomatology, cognitive impairment, functional limitations, and metabolic conditions in Puerto Ricans. Further investigation includes the potential modification of these associations by nutritional status, especially for dietary fat, B vitamins and antioxidants, by genetic variation, and by sources and type of social and community support. The study was approved by the Institutional Review Board at Tufts Medical Center and Northeastern University. All participants provided written informed consent.
Participants are recruited from the Greater Boston area using door-to-door enumeration and community approaches. Data from the 2000 Census was used to identify census tracks with at least 25 Puerto Rican adults, ages 45-75 years. Within these, randomly selected census blocks with 10 or more Hispanics, ages 45-75 years, were enumerated door-to-door. Blocks were visited at least three--and up to six--times, on different days of the week, including weekends, and at varying times of day, including evenings. Households with at least one eligible adult were identified. One participant per qualified household was randomly invited to participate.
Similar to other studies [20-22], multiple recruitment strategies were used. In addition to the door to door enumeration, participants were identified by random approach during community festivals/fairs and events sponsored by local community organizations, through referrals, and through calls to the study office from flyers distributed at community locations, or from radio or television spots about the study. The community partner, La Alianza Hispana, is a non-profit organization serving Latinos in the Boston area. They provided advice and assistance with the recruitment and retention efforts, and continue to serve as a liaison with the participants.
Eligible participants must be of self-identified Puerto Rican descent, able to answer questions in English or Spanish, ages 45-75 years, and living in the Boston, MA metropolitan area at the time of the study. Individuals who were unable to answer questions due to serious health conditions, planned to move away from the area within two years, or who had a low Mini Mental State Examination (MMSE) score (≤ 10) were excluded. Interviews were scheduled after the initial screening contact. Participants were provided with a written reminder of the interview date, and were called 1-2 days prior to the interview to remind them. For those who cancel, rescheduling was attempted at least five times, after which participants were considered de-facto refusals.
Baseline questionnaires and tests were administered by trained, bilingual interviewers in the participant's home. After obtaining informed consent, neuropsychological tests were conducted to identify participants that may need assistance from a proxy, or be excluded due to low MMSE score. Those who qualified completed the rest of the interview. Participants were given a 12-hour urine collection cup and two saliva tubes, with procedures for collection and instructions on fasting for the next day's blood draw. A certified phlebotomist returned to collect the samples and draw blood in the home.
All interviewers were thoroughly trained by experienced staff to administer the questionnaires and to perform measurements following procedures from National Health and Nutrition Examination Survey (NHANES) II  and the MacArthur Studies of Successful Aging . Retraining and review sessions, including checks on scoring of tests and scales, were conducted periodically. Each new interviewer was required to observe several interviews by experienced interviewers and to practice repeatedly before collecting data. Completed interviews were self- and peer-reviewed prior to database entry.
Participants provided information on age, education level, household income, migration, acculturation, employment history, family size and food security. These questionnaires were designed based on NHANES III [25,26], the Hispanic Health and Nutrition Examination Survey [27,28] and the National Health Interview Survey Supplement on Aging . Poverty status was computed using the poverty thresholds released each year by the US Census Bureau . Each participant's total annual household income was compared to the threshold based on the age of the head of household, participant's family size, and year of interview. If total household income was less than this threshold, the participant was considered to live in poverty.
Acculturation was captured by a set of questions on language use in a number of everyday activities [31,32]. This language-based questionnaire was adapted from the Bi-dimensional Acculturation Scale for Hispanics (BAS), which focuses on language preference in various settings . This allows for the possibility that acculturation entails the acquisition of American cultural traits without displacing Hispanic ones. The scale yields two scores which rank participant's acculturation in the Hispanic domain and the non-Hispanic domain; a value of 100% represents a fully acculturated participant in the non-Hispanic domain who speaks fluent English. A psychological acculturation scale that focuses on psychological attachment to either culture was also administered. The scale was validated with three different samples of Puerto Ricans from the greater Boston area .
Participants were asked to self-report whether they have been diagnosed with a specific list of chronic conditions. Detailed information on prescription and over-the-counter medications was collected by asking participants to show the bottles for medications they currently take. Participants provided health insurance information and self-rated their health status and satisfaction with health care practices. Frequency, history, and type of alcohol consumption and smoking were assessed. Current physical activity was captured using a modified Paffenbarger questionnaire of the Harvard Alumni Activity Survey [35,36], which was effectively tested in an elderly Puerto Rican population . A physical activity score was calculated as the sum of hours spent on typical 24-hour activities (heavy, moderate, light, or sedentary activity, and sleeping) multiplied by weighing factors that parallel the rate of oxygen consumption associated with each activity.
Standing height, knee height, weight, and waist and hip circumferences were measured in duplicate [23,37]. Body mass index (BMI) was calculated using weight (Kg) divided by height (m) squared. Systolic and diastolic blood pressures were measured in duplicate, at three time points during the interview. The second and third readings were averaged. Detailed methodology is included in Additional File 1.
Participants were asked to report difficulty performing daily activities, with modified Katz Activities of Daily Living (ADL) and Instrumental Activities of Daily Living (IADL) scales . The twelve ADL and six IADL items have been used effectively in a previous cohort of elderly Puerto Ricans . Additional physical performance tests, which measure balance, gait, chair stands, foot taps and manual ability, were completed, following tests used in the MacArthur Studies of Successful Aging .
A comprehensive neuropsychological examination assessed specific impairments in cognitive functioning. Tests were selected based on evidence of validity in Spanish-speaking populations, and to evaluate higher cognitive functioning to minimize floor effects. They include the MMSE for general function , the word list learning test  for verbal memory, the Stroop  for mental processing speed, digit span  for working memory, verbal fluency  for executive function with language, and clock drawing  and figure copying  for visuospatial function. Cognitive impairment categories, as defined by MMSE scores, were adjusted for educational level, as this correction optimizes the test . For all levels, considerable impairment was defined as a score of 11 through 17; mild impairment includes score of 18 through 21 for participants with middle school, 18-23 for those who completed high school, and 18-24 for those with college or graduate education. Higher scores than those cutoff values by educational attainment were deemed as having no impairment.
Depressive and anxiety symptoms were assessed using the Center for Epidemiology Studies Depression (CESD) Scale [45-47]. The CESD has shown consistency and validity in older adults . It has also been used with Hispanics , including Puerto Ricans , with good reliability. The Spanish versions of the Life Events Questionnaire [49,50] and the Norbeck Social Support Questionnaire  were used to assess life and psychosocial stress. The Perceived Stress Scale measures the degree to which one's life is viewed as stressful , with higher values representing higher perceived stress. The scale has been satisfactorily tested in other Spanish-speaking groups [53,54]. A second stress scale, developed through qualitative interviews with a subset of participants, was also administered in the second wave of interviews.
Dietary intake was assessed using a semi-quantitative food-frequency questionnaire (FFQ) with 126 items, adapted and validated for this population . The FFQ, based on the National Cancer Institute-Block FFQ format, was revised to include appropriate foods and portion sizes, and was shown to capture intakes reported in 24-hour recalls more accurately than the original questionnaire, both in total nutrient estimates and in ranking of individuals . This FFQ has been validated against plasma carotenoids , vitamin E  and vitamin B12  in Hispanics aged ≥ 60 years. Those with energy intakes < 600 or > 4800 kilocalories and/or > 10 questions blank on the FFQ were excluded from dietary analyses.
Detailed methodology is included in Additional File 1. Blood samples were analyzed for complete blood counts, creatinine, albumin, plasma lipids, total carotenoids, vitamin C, folate, vitamin B12, pyridoxal-5'-phosphate, homocysteine, methylmalonic acid, C-reactive protein, glucose, insulin, glycosylated hemoglobin, blood urea nitrogen, and dehydroepiandrosterone sulfate. A 12-hour urine sample was analyzed for cortisol, creatinine, epinephrine and norepinephrine. Saliva samples were used for measurement of salivary cortisol.
Diabetes status was defined as fasting plasma glucose ≥ 126 mg/dL or use of medication . Hypertension was defined as blood pressure ≥ 140/90 mmHg or use of medication . BMI was used to classify weight status, with BMI 25-29.9 as overweight, 30-39.9 as obese class I and II, and ≥ 40 as extremely obese . Waist circumference > 102 cm in men or > 88 cm in women was defined as high . Depressive symptomatology was defined as CESD score ≥ 16 .
DNA extraction and genotyping methods have been previously described . Briefly, genomic DNA was isolated from blood using QIAamp DNA Blood mini kit (Qiagen, Hilden, Germany). Genetic polymorphisms were genotyped with Applied Biosystems TaqMan SNP genotyping system . Genotyping error rate was < 1%, as estimated by internal quality control and independent, external laboratories. Genes and polymorphisms were selected by bioinformatics assessment, based on previously reported associations or knowledge of their function in known biological mechanisms. Over 140 autosomal, diallelic polymorphisms, from 35 different genes have been genotyped.
Two-year follow-up is ongoing at time of writing. All subjects are contacted and asked to participate in a follow-up visit after two years, to repeat all questionnaires and measurements. Participants are called every six months to update contact information (at which point they update a life events inventory questionnaire), and receive holiday and birthday cards and occasional newsletters with updates on the study. At baseline, participants were asked to provide the names of two contacts who could locate them if they moved. These persons are contacted if the participant cannot be reached directly after several attempts. When participants cannot be contacted by phone, letters are sent requesting that they call the study. When there is no response, a staff member visits the home. Awareness of the study in the general community is fostered by participating in community events, attending meetings of community agencies to present information on the study, encouraging articles in both Spanish and main newspapers, and participating in media appearances.
Statistical analyses for this report were completed using the SAS System for Windows (version 9.1, SAS Institute, Inc, Cary, NC). Descriptive analyses to examine differences between sex and age groups (45-59 and 60-75 years) were performed using chi-square analyses for categorical variables and t-tests for continuous variables with normal distributions. Fisher's exact tests were used for variables with an expected cell count of less than five. P values were calculated and a significance level of < 0.05 was used. All tests were two-sided.
The majority of participants were identified through door-to-door enumeration (77.4%), with the rest recruited through community events (9.8%), referrals (7.2%), and calls to the study office (5.6%) (Figure (Figure1).1). From June 2004 to October 2009, a total of 2,170 Puerto Rican age-qualifying adults were identified. Of these, 77 met the exclusion criteria, and 2,093 were invited to participate. From those invited, 1,811 (86.5%) agreed to be interviewed. Primary reasons for declining included not being interested in the study, too busy, and refusal of blood draw. Those who declined were more likely to be older (58.4 versus 56.7 years, data not shown) and had lived on the US for more years (32.9 versus 28.8 years, data not shown). No significant differences in sex, language spoken or birthplace were observed between those participating and those declining. Among those agreeing to participate, nine participants were excluded due to low MMSE score (0.5%), 15 (0.8%) dropped out, 36 (2.0%) were lost before interview, and 251 (13.9%) were never interviewed due to persistent unavailability. Therefore, 1,500 of 1,802 eligible participants who initially agreed to participate (83.2%), or of 2,084 eligible participants who were initially invited (72.0%), completed the baseline interviews. Results presented in this report include 1,357 participants with completed and cleaned baseline interviews at the time of writing.
Those recruited through door-to-door enumeration, relative to community events or calls in response to advertising the study, were significantly older, more likely to be women, have lower education, less acculturated, and less likely to be employed than those recruited by community approaches (data not shown). They did not differ by poverty status, BMI, or prevalence of type 2 diabetes. Based on year 2000 Census tract data, these recruitment strategies included access to 78% of the Puerto Ricans, aged 45-75 years, living in the Boston area towns with participants in the study. When limited to towns with 5 or more participants, coverage was close to 96% for the door-to-door enumeration approach.
Once recruited, completion of interviews was time-consuming and expensive. Appointments needed to be rescheduled approximately 40% of the time, usually because the participant was not home at the scheduled time, or cancelled at the last minute. Among the most common reasons for cancellations were participant's failure to remember the interview appointment, a conflicting medical appointment, illness or death of a family member, and reported illness by the participant.
The majority of the sample was female (70%). Mean ages were 57.2 years for men and 57.9 years for women. Older Puerto Rican adults (60-75 years) reported fewer years of education than younger adults (45-59 years); over 60% of older adults had < 8th grade education (Table (Table1).1). Approximately 50% of adults in all age categories fell below the poverty level, with older women more likely to be living in poverty. Only one-third of men and one-quarter of women in the younger group, and even fewer older adults, were employed at the time of the study. Residential stability was 50% or more for all groups except for younger men (35.1%).
The participants in this Puerto Rican cohort had lived in the US for over three decades, on average; of those aged 60-75 years, men had lived in the US longer than women (42.2 versus 36.0 years). Language-based acculturation scores were significantly lower in the older than younger age group for both men and women. In the younger age group, men were significantly more acculturated than women. Psychological acculturation score showed similar patterns as the language-based scale by age, but men and women had comparable scores.
To evaluate life events and social support, participants were asked to identify important persons in their life and to indicate their perception of how these important persons could support them emotionally or assist them in time of need. Significant differences by sex were found among the younger cohort in the size of the social networks and in the perception of availability of emotional and functional support (Table (Table2).2). Younger women reported larger networks, but also a lower perception of existing emotional and functional support from these networks than did men in the same age category. These differences were not found among the older cohort, suggesting that some of the earlier differences may be related to life cycle events like marriage, work, and the availability of kin. Women's social networks included a larger number of blood relatives than the social networks of men (3.7 versus 3.2, respectively). Differences between men and women in the presence of friends, other type of relatives, and others in their networks were not significant (data not shown). Reported sources of social support in this population were limited to close family and friends, with few social contacts, on average, beyond the latter two categories.
Significantly more men than women in both age groups were overweight, while a greater percent of all women fell into the extreme obesity category (Table (Table3).3). Close to 80% of women, in both age groups, had abdominal obesity. Overall, participants were relatively inactive; fewer than 12% of men and 4% of women participated in moderate or vigorous activity. Men were more likely to smoke cigarettes or be heavy drinkers than women. About one in five participants used multivitamins, with no significant differences in use by sex or age group. Men consumed significantly more energy and dietary fiber, but less carbohydrate, vitamin B6 and potassium than women across both age groups (Table (Table4).4). Participants in the older age category had lower energy intake, and consumed less fat but more carbohydrate than younger participants.
A quarter of women aged 45-59 years and over a third aged 60-75 years reported considerable physical impairment (Table (Table5).5). Cognitive impairment was significantly higher in women than in men in the older age category; considerable impairment was more prevalent in the older than the younger group. The majority of participants self-reported their health status as fair, but more women reported poor health status. Fifty-one percent of women and 45% of men aged 60-75 years had type 2 diabetes (Table (Table6).6). More than three-quarters of all older participants had hypertension. A higher proportion of younger than older participants had depressive symptomatology, but prevalence was high for all groups. Self-report of cardiovascular disease was significantly higher for the older group of Puerto Ricans; there were no significant differences by sex. Approximately 70% of older women self-reported diagnosis of arthritis.
At the point of this writing, 951 (78.7%) two-year follow-up visits have been completed, from the 1,209 participants who have reached their follow-up scheduling time point (data not shown). Of non-completing participants (258), 85 (7.0%) dropped out, 52 (4.3%) were lost to follow-up, and 17 (1.4) were deceased. The remaining 104 participants are being actively rescheduled due to frequent change of residence within the city, circular migration to and from Puerto Rico, and disconnected telephones. Although exact two-year timing of follow-up is difficult, participants are continuously being contacted. Increased participation and retention has been attained throughout the course of the study, as it becomes better known in the community. The remaining 291 baseline participants will be scheduled at their appropriate follow-up time. Assuming the same participation rate as baseline, a final two-year follow-up of at least 1,200 participants is expected.
The experience of the BPRHS illustrates the challenges of conducting research in a Hispanic ethnic subgroup, and demonstrates the disparities faced by this community. The study has recruited 1,500 older adult Puerto Ricans, but considerable effort to obtain completed interviews has been required. Several studies have reported difficulties in recruiting minority research participants [65-68], especially Latino men . It is possible that with door-to-door enumeration, women are more likely to be at home and therefore more frequently approached. They also appear to be more willing to participate than men, although this was not statistically significant in this study.
After enumeration, re-contacting individuals posed significant challenges. A key factor in the eventual success in locating individuals was recording information of close contacts. However, in many cases, field staff needed to return to the neighborhood to locate the individual. Recruitment completion was further delayed by frequent cancellations of appointments. These challenges led to loss of staff time and effort, and greatly increased the study cost over that originally estimated. Similarly, Eakin et al. reported the need to hire extra phone staff in order to increase retention of Latinos . Interestingly, main reasons for cancellation included medical appointments and illness of the participant or a relative, which is consistent with the observed high prevalence of disease, and the sharing of burden among socially connected individuals.
Mistrust of scientific investigations is frequently reported as a major barrier to recruitment in minority participants . Several strategies were used to facilitate recruitment, including employing a bilingual and ethnically diverse staff, and partnering with a local community organization. Recruitment at community events increased the study's visibility and involvement in the community, and enabled staff to obtain updated contact information for enrolled participants. Use of the media also reinforced the legitimacy of the study. Because the total community is relatively small, receptivity increased over time.
Efforts to keep the participants' trust and engagement in the study have helped sustain a high retention during two-year follow-up. As the study moves forward, questions on progression and mechanisms of diseases may be answered more accurately. Little is known about the environmental influences and life events of elderly Puerto Ricans living in the US; thus, possible cohort effects require further consideration. The wide age range of this group, constant migration patterns to/from Puerto Rico, limited social networks, and low residential stability may limit assumptions about cohort effects.
As with most epidemiological studies, selection bias could be operating in this study. For example, as those declining participation in the study were living in the US longer that those participating, possible selection bias by acculturation, which is highly correlated to years living in the US, may occur. Still, there was low acculturation in this sample, suggesting that such bias may not exist. The door-to-door recruitment method may have introduced selection bias, as participants recruited with this method, who comprised the majority of this cohort, had somewhat differing characteristics than those recruited through community events. The study followed exhaustive protocols to identify participants at home, making a great effort to expand recruitment with various strategies; the addition of participants from community approaches likely improved the representativeness for the study, as individuals who may have been seldom at home were included. Notably, the majority of Puerto Ricans aged 45-75 years identified by Census tracts, lived in neighborhoods and communities from which the study recruited.
Data from the 2000 Census show that, of Puerto Ricans aged 45-75 years living in high-density Hispanic blocks in Boston, 75% were 45-59 years, and 25% were 60-75 years . Overall, the sample of this study was somewhat older, with about two-thirds in the younger and one-third in the older age range. When stratified by recruitment method, the age distribution more closely resembled that of the Census findings for those approached through flyers, community events or referrals (70% aged 45-59 years), whereas door-to-door recruitment yielded 60% in the younger category. Education levels for study participants were similar to those identified for Census data for Puerto Ricans in Boston; yet the study had fewer women in the older group that held a current job relative to 2000 Census data, while a greater number of men in the older age category reported working in this sample. This difference may be partly due to economic changes that have occurred since the year 2000. This sample of 1,500 individuals represents a fairly large proportion (15%) of the 10,241 Puerto Ricans in this age range living in the towns that we recruited from, as of 2000. Although this may suggest that a representative sample for this population was likely captured, the limited areas and approaches for recruitment may have reduced representativeness. Still, the results should be reasonably generalizable to similar communities of Puerto Rican adults living in high density urban areas in the US.
Hispanic subgroups are often combined together in health research; however, this practice may obfuscate important differences in subgroups. Though limited, accumulating studies provide evidence that health disparities differ considerably by subgroup [7,8,12-18,70]. The results of this study support observations that Puerto Ricans on the US experience considerable health disparities which exceed those reported for NHW or other Hispanic subgroups, including the more commonly studied Mexican Americans. The prevalence of physical and cognitive disability, type 2 diabetes, obesity, depressive symptomatology, hypertension, and self reported heart disease were higher in this sample, in relation to published reports for similarly-aged Mexican Americans [3,71-73]. Notably, the high prevalence of these conditions was observed even for those in the younger age category. For example, the prevalence of obesity in this sample of Puerto Rican men and women, aged 45-59 years, (43% and 60.5%, respectively) was higher than that reported by NHANES 2001-2004 for Mexican Americans in the same age range (36.3% and 52.2%, respectively) . One caveat of this type of comparisons is that differences in survey methodology and year of data collection may affect the interpretation of the comparison.
In conclusion, the BPRHS is the largest and most comprehensive study conducted to date on older adult Puerto Ricans living in the US, exclusively. It adds considerably to the efforts of other studies conducted in Hispanic elders, including the Health and Retirement Study, the Hispanic Established Populations for Epidemiologic Studies of the Elderly, and the Hispanic Community Health Study/Study of Latinos. This group experiences chronic health conditions at higher prevalence than those reported nationally for Mexican Americans or for NHW. The reasons for these health disparities remain largely unexplained, but illustrate the critical need for more research on the dynamics involved in these poor health outcomes. They also underscore the need to investigate Hispanic subgroups as unique cohorts, and to be careful when using the encompassing terms "Hispanic" or "Latino" when presenting health issues. The recruitment process portrays the challenges and opportunities involved in the enrollment of minority groups in epidemiological and clinical research, and may help strengthen future efforts for other studies. Understanding the unique needs of Puerto Ricans will inform interventions and public health practice, so that resources may be used prudently, and health disparities may be reduced. Improved scientific understanding of the etiology and progression of chronic conditions may also be attained.
ADL: Activities of Daily Living; BMI: Body mass index; BPRHS: Boston Puerto Rican Health Study; CESD: Center for Epidemiology Studies Depression scale; FFQ: Food frequency questionnaire; IADL: Instrumental Activities of Daily Living; MMSE: Mini mental state examination; NHANES: National Health and Nutrition Examination Survey; NHW: Non-Hispanic white; US: United States.
The authors declare that they have no competing interests.
KLT, the principal investigator, designed the study, directed its implementation, and supervised data analysis and interpretation; JMattei and SEN analyzed the data, interpreted the results, and contributed to writing the manuscript; BMC assisted with data analysis and the manuscript draft; JMendez coordinated the field activities and provided portions for the manuscript text; JN and JG collaborated with data analysis, interpretation of results, and portions of the text; JMO and LMF, co-principal investigators, helped design the study, conduct portions of its implementation, and interpret results. All authors reviewed the manuscript and approved the final version.
The pre-publication history for this paper can be accessed here:
Detailed Methodology for the Boston Puerto RicanHealth Study. A full description of the protocols used to obtain anthropometric and biochemical (blood, urine, saliva) measures.
This study is supported by the National Institutes of Health-National Institute on Aging grant number P01-AG023394, and by the United States Department of Agriculture, Agriculture Research Institute agreement number 58-1950-7-707.