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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Breast Cancer Res Treat. Author manuscript; available in PMC 2010 November 1.
Published in final edited form as:
Breast Cancer Res Treat. 2009 November; 118(1): 213–228.
Published online 2008 October 26. doi: 10.1007/s10549-008-0227-7

Fig. 6

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Effect of fish oil and DHA and EPA on PTEN expression. a Tumor lysates from lab chow and fish oil fed mice were immunoblotted with PTEN and tubulin antibodies respectively. b Quantification of the PTEN expression in (panel a). Mean ± SE of three independent animals is shown; *P = 0.02 vs. lab chow fed animals. c, d 2 μg of total RNA isolated from MDA MB-231 cells incubated with DHA (panel c) or EPA panel d as indicated were used in real time qRT-PCR to detect PTEN mRNA as described in the Experimental procedures. The expression of mRNA was normalized to GAPDH. Mean ± SE of three measurements is shown. *P < 0.05 vs. control in (panel c); *P < 0.01 vs. control in panel d; by ANOVA. e, f Lysates of MDA MB-231 cells treated with DHA (panel e) or EPA (panel f) were immunoblotted with PTEN and tubulin respectively

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