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F1000 Biol Rep. 2010; 2: 20.
Published online 2010 March 15. doi:  10.3410/B2-20
PMCID: PMC2847284
NIHMSID: NIHMS188136

Recent advances in polyubiquitin chain recognition

Abstract

Polyubiquitin chains are regulatory signals for a wide array of biological processes. Recent structural studies reveal novel modes of polyubiquitin chain recognition and implicate the diverse repertoire of interactions in providing the specificity of polyubiquitin recognition.

Introduction and context

As the name implies, ubiquitin (residues 1-76) is a ubiquitous signaling molecule for a wide array of biological processes, including protein degradation, assembly of signaling complexes, and DNA damage repair. When attached to proteins, ubiquitin or polyubiquitin chains act as ‘codes’ to direct suitable protein-protein interactions to effect or regulate specific cellular functions. Ubiquitin is conjugated to target proteins or itself through an isopeptide linkage between the side chain amino group of lysine (Lys) residues and the C-terminal carboxyl group of ubiquitin. This process is achieved by the sequential action of ubiquitin-activating enzymes (E1), ubiquitin-conjugating enzymes (E2), and ubiquitin ligases (E3). The ubiquitin ‘codes’ on cellular proteins can be monoubiquitin, multiple monoubiquitins, or polyubiquitin chains of diverse length and linkage. There are seven Lys residues on ubiquitins (Lys6, Lys11, Lys27, Lys29, Lys33, Lys48, and Lys63), allowing for seven possible homotypic linkages and multiple possible mixed chains. The best characterized polyubiquitin linkage types are those mediated by Lys48 and Lys63, with the former known in targeting proteins for proteasomal degradation and the latter involved in multiple signaling processes, in particular nuclear factor-kappa-B (NF-κB) activation. Recent studies identified a new linkage type of polyubiquitin chains: the linear linkage formed between the N-terminal amino group and the C-terminal carboxyl group (see ‘Interactions of NEMO with diubiquitins: both revelations and confusions’ section below). Like phosphorylation, ubiquitination is highly reversible through the action of deubiquitinating enzymes (DUBs) and therefore acts as a transient and versatile signaling element.

Ubiquitin recognition by the collective entity of ubiquitin-binding domains (UBDs) is at the center of ubiquitin-mediated signaling. So far, investigators have discovered over 20 types of UBDs, such as ubiquitin-associated domains (UBAs), ubiquitin-interacting motifs (UIMs), zinc fingers (ZFs), and Jab1/MPN domains for the metalloprotease class of DUBs. Monoubiquitin recognition has been extensively studied and reviewed elsewhere [1,2], however, only limited examples of polyubiquitin recognition were known until very recently. Here, we will summarize recent advances in understanding the molecular basis of diubiquitin recognition and, in some cases, the extrapolation to longer polyubiquitin chains.

Major recent advances

A very first example of diubiquitin recognition is provided by the nuclear magnetic resonance (NMR)-derived structure of Lys48-linked diubiquitin in complex with a UBA domain of hHR23A, a protein implicated in the modulation of polyubiquitin interaction with the proteasome [3]. In this structure, the distal and the proximal ubiquitin molecules use the conserved hydrophobic patch centered on residue Ile44 to grip onto either side of the UBA domain, which consists of a compact triple-helical bundle (Figure 1A). In a diubiquitin, the distal ubiquitin provides the C-terminal tail while the proximal ubiquitin provides the amino group for the isopeptide bond. The past year or so has seen an explosion of structural information on the recognition of Lys63-linked or Lys48-linked or linear diubiquitins. The new structures of UBD-diubiquitin complexes from different cellular processes provide an expanded view of the diversity of such interactions.

Figure 1.
Structures of ubiquitin-binding domain (UBD)-diubiquitin complexes

Interactions of NEMO with diubiquitins: both revelations and confusions

NEMO is the regulatory subunit of the IKK (IκB kinase) complex for NF-κB activation. Recent studies have shown that the UBAN (ubiquitin binding in ABIN [A20-binding inhibitor of NF-κB activation] and NEMO [NF-κB essential modulator]) domain directly interacts with linear polyubiquitin chains [4-7]. The UBAN motif of NEMO forms a dimeric coiled coil and uses both chains for linear diubiquitin interactions. Structure-based mutagenesis and NMR studies revealed that the Ile44 patch and the C-terminal tail of the distal ubiquitin in linear diubiquitin are most critical for UBAN interaction [6]. The crystal structure of the UBAN-linear diubiquitin complex revealed interfaces that are similar to the NMR-based studies [7]. However, despite the measured stoichiometry in solution of one UBAN dimer per linear diubiquitin [6,8], the crystal structure showed that two dibubiquitin molecules interact symmetrically on each side of the UBAN dimer [7] (Figure 1B). It is possible that only one side of the dimeric UBAN is bound with diubiquitin in solution and that the other side was forced to interact with another diubiquitin molecule under the crystallization condition. Consistent with this assessment, it appears that at high diubiquitin concentrations, there is a second, weaker binding site [8].

The UBAN motif of NEMO also directly interacts with Lys63-linked polyubiquitin [4-6,9], albeit at a much lower affinity than with linear diubiquitin [6]. Similar mutagenesis and NMR-based methods revealed that the Ile44 patch of the proximal ubiquitin in Lys63-linked diubiquitin and the C-terminal tail of the distal ubiquitin provide the critical binding energy for the UBAN interaction [6]. In addition, the stoichiometry of the interaction was shown to be one UBAN dimer per Lys63-linked diubiquitin [6]. In contrast to the crystal structure of the UBAN-linear diubiquitin complex [7], the crystal structure of the UBAN-Lys63-linked diubiquitin complex showed that the diubiquitin interacts with only one side of the UBAN dimer [9] (Figure 1C). In fact, only one ubiquitin molecule in the diubiquitin, either the proximal or the distal ubiquitin, interacts with UBAN in the crystal lattice, and this is different from the higher energetic role of the proximal ubiquitin shown by mutagenesis experiments in solution [6].

Recent NMR and cellular studies showed that the C-terminal ZF of NEMO is also a functional UBD and plays critical roles in NF-κB activation [10]. Remarkably, while neither the UBAN nor the ZF domains shows any preference for K63-linked polyubiquitin chains, they together form a bipartite high-affinity K63-specific UBD [11]. Further structural studies are required to reveal the molecular basis for this specificity.

Recognition of Lys63-linked diubiquitin by UIMs of RAP80

Receptor-associated protein 80 (RAP80) plays a key role in recruiting the Abraxas-BRCC36-BRCA1-BARD1 complex to DNA damage foci for DNA repair by possessing an Abraxas-interacting region. It also contains tandem UIMs, which are single-helical motifs, for interaction with Lys63-linked polyubiquitin. The crystal structure of the tandem UIMs of RAP80 in complex Lys63-linked diubiquitin revealed that the two UIMs of RAP80 and the inter-UIM region form a continuous helix [12,13]. The proximal and the distal ubiquitins of the Lys63-linked diubiquitin interact with UIM1 and UIM2, respectively, in a manner that involves the Ile44 patches (Figure 1D). The specific spacing between the UIMs appears to be critical in the specificity of the interaction for Lys63 linkage, although no direct interaction is present between RAP80 and the Lys63-isopeptide bond [12,13].

Concerted polyubiquitin interactions by ubiquitin receptors in the proteasome

Lys48-linked polyubiquitin recognitions by the two ubiquitin receptors in the proteasome, S5a and Rpn13, have been revealed by elegant crystallographic and NMR studies [14,15]. S5a contains two independent UIMs and its UIM2 preferentially interacts with the proximal ubiquitin in a Lys48-linked diubiquitin (Figure 1E). Rpn13 contains a pleckstrin-like receptor for ubiquitin (Pru) domain and uses loops rather than secondary structural elements for ubiquitin interaction [14]. Dibuqiutin recognition by Rpn13 is mediated mostly by binding to the proximal ubiquitin at the Ile44 patch and to the Lys48 linkage between the proximal and the distal ubiquitin (Figure 1F). It is proposed that in the intact proteasome, Rpn13 and S5a interact simultaneously with tetraubiquitin or a longer polyubiquitin to accomplish polyubiquitin targeting to the proteasome [15].

Specificity for different linkages in DUBs

AMSH-like protease (AMSH-LP) is a Jab1/MPN domain-containing DUB with a Zn-coordinating catalytic core. The crystal structure of Lys63-linked diubiquitin in complex with AMSH-LP revealed that the distal ubiquitin uses the Ile44 hydrophobic patch for interaction with AMSH-LP while the proximal ubiquitin uses an alternative surface [16]. Most strikingly, the surface of AMSH-LP forms a catalytic groove that accommodates the Lys63 side chain of the proximal ubiquitin and the isopeptide-linked C-terminal tail of the distal ubiquitin (Figure 1G). Only the Lys63-linked proximal ubiquitin can bind in the proper orientation for catalysis. Sequence similarity also suggests that the specificity of AMSH for Lys63-linked chains is derived primarily from the interactions with the proximal ubiquitin. Bidentate recognition of both proximal and distal ubiquitins has also been shown as the mechanism of specificity for K48 linkage in the ovarian tumor domain-containing DUB, Otubain 1 [17].

Specific recognition of Lys63-linked diubiquitin conformation by TAB2 and TAB3

Activation of the kinase TAK1 in JNK and NF-κB signaling pathways requires interaction with Lys63-linked polyubiquitin chains by the TAK1-binding proteins, TAB2 and TAB3. TAB2 and TAB3 recognize Lys63-linked chains, but not linear chains, using their C-terminal Np14 zinc finger (NZF) domains. Crystal structures of the TAB2 NZF domain in complex with Lys63-linked diubiquitin or triubiquitin and the TAB3 NZF domain in complex with Lys63-linked diubiquitin reveal that each NZF domain interacts with a diubiquitin using two distinct binding sites and that multiple NZF domains could recognize longer polyubiquitin chains successively [18,19] (Figure 1H). The NZF domains recognize the Ile44 hydrophobic patches of both the proximal and the distal ubiquitins in a diubiquitin. Interestingly, the Lys63-linked isopeptide bond is not involved in the interaction. In contrast, the specificity of the interaction for Lys63-linked chains versus linear chains is conformational as the Met1 residue of the proximal ubiquitin is too far away from the C-terminus of the distal ubiquitin in the diubiquitin complexes with the NZF of TAB2 and TAB3.

Recognition of Lys63-linked diubiquitin by a specific antibody

Interestingly, in an effort to reveal the role of polyubiquitin chains of specific linkages in diverse biological processes, antibodies were developed to recognize Lys63 versus Lys48 linkages [20]. The crystal structure of a Fab fragment of a specific antibody in complex with Lys63-linked diubiquitin revealed that the Fab interacts with the diubiquitin at either side of the isopeptide linkage with limited involvement of the Ile44 patch of either ubiquitin (Figure 1I). It appears that the Fab specifically recognizes a conformation of the diubiquitin that is compatible with the Lys63, but not the Lys48, linkage.

Future directions

The recent structural studies may have pointed out a number of rules or the lack of them in diubiquitin recognition. First of all, diverse classes of UBDs are capable of diubiquitin recognition and achieve the specificity in their own manners. Second, the Ile44 hydrophobic patch is often crucial for diubiquitin recognition, as is the case for monoubiquitin interaction, and the spatial separation of UBDs in a protein may facilitate the simultaneous interaction with the Ile44 patches in a diubiquitin. Third, the linkage residues themselves may or may not be directly involved in UBD interaction. Fourth, and importantly, intrinsic conformational preferences in solution of polyuibiquitin chains of different linkages are important determinants in the specificity of polyubiquitin chain recognition. Structural studies of diubiquitins and tetraubiquitins have revealed that Lys48-linked chains in general prefer a more compact structure than either Lys63-linked chains or linear chains [21-27]. It should be cautioned, however, that crystal packing exerts significant influences in polyubiquitin chain conformation [25,27]. Overall, it appears that nature has developed a diverse repertoire of interactions to provide specificity of polyubiquitin recognition in biological processes.

Future structural studies on polyubiquitin chain interaction may reveal recognition of UBDs with longer-chain polyubiquitins. It is known that diubiquitin is often not sufficient for eliciting the intended biological responses such as in proteasomal targeting and NF-κB activation. One reason for this requirement has been demonstrated for simultaneous polyubiquitin chain recognition by two UBDs, which sets the chain length threshold [28]. Similarly, long polyubiquitin chains may serve as extended scaffolds to mediate recruitment of multiple copies of the signaling proteins to induce oligomerization. Another reason for the requirement of longer chains may be avidity, which increases the strength of the interactions. Studies on long polyubiquitin chains may be challenging but will certainly transform our current thinking in polyubiquitin signaling.

Acknowledgments

The work was supported by a National Institutes of Health grant (R01AI079260) to HW.

Abbreviations

AMSH-LP
AMSH-like protease
DUB
deubiquitinating enzyme
Lys
lysine
NEMO
NF-κB essential modulator
NF-κB
nuclear factor-kappa-B
NMR
nuclear magnetic resonance
NZF
Np14 zinc finger
RAP80
receptor-associated protein 80
UBA
ubiquitin-associated domain
UBAN
ubiquitin binding in ABIN (A20-binding inhibitor of NF-κB activation) and NEMO (NF-κB essential modulator)
UBD
ubiquitin-binding domain
UIM
ubiquitin-interacting motif
ZF
zinc finger

Notes

The electronic version of this article is the complete one and can be found at: http://f1000.com/reports/b/2/20

Notes

Competing interests

The authors declare that they have no competing interests.

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