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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Immunol. Author manuscript; available in PMC 2010 March 30.
Published in final edited form as:
PMCID: PMC2847272
NIHMSID: NIHMS188067

Cutting Edge: IL-7-Independent Regulation of IL-7 Receptor α Expression and Memory CD8 T Cell Development1

Abstract

Expression of IL-7Rα on a subset of Ag-specific effector CD8 T cells is believed to identify memory cell precursors. However, whether IL-7 regulates IL-7Rα expression in vivo and is responsible for selective survival of IL-7Rα+ effector cells is unknown. Our results show that in the absence of IL-7, IL-7Rα expression was extinguished on the majority of CD8 T cells responding to virus infection, sustained on a subset of effector cells transitioning to memory, and expressed at high levels by memory cells. Additionally, an IL-7-deficient environment was capable of supporting bcl-2 up-regulation and memory cell development in response to virus infection. Thus, IL-7Rα regulation occurs independently of IL-7 in responding CD8 T cells, indicating that CD8 memory T cell precursors are not selected by IL-7/IL-7Rα interactions.

The cytokine IL-7 is central to development and survival of T lymphocytes as deficiencies in either IL-7 or IL-7Rα block the development of the T cell lineage (1) and IL-7 signaling is required for the survival of naive T cells and their homeostatic proliferation in immunodeficient hosts (24). The regulation of survival of CD8 T cells is due in part to bcl-2 induction through IL-7R signaling (5, 6). Although IL-7Rα is highly expressed by naive and memory CD8 T cells, most responding effector cells transiently down-regulate IL-7Rα (2). Also, a small population of effector CD8 T cells retain or re-express IL-7Rα (7), and this population is thought to represent memory T cell precursors. In support of this concept are studies showing that when early inflammation is blocked following infection, the contraction phase of the immune response is bypassed by IL-7Rα-expressing cells (8). However, immunization with peptides in adjuvant or peptide-pulsed dendritic cells (DCs)4 induces increased numbers of IL-7Rα+ effector cells that do not transit to the memory compartment. Thus, IL-7R expression does not necessarily identify CD8 memory T cell precursors.

The potential survival advantage for IL-7Rα+ memory CD8 T cell precursors implies that IL-7 binding to IL-7Rα modulates the receptor. Although the inclusion of IL-7 as an adjuvant in vaccination protocols has been proposed to selectively expand memory cells (9), this notion implies that IL-7 selects IL-7Rα+cells into the memory pool. Indeed, IL-7 has been shown to regulate IL-7Rα expression in vitro (10, 11). However, whether IL-7 regulates IL-7Rα expression following CD8 T cell activation in vivo is unknown. We now show that regulation of IL-7Rα expression is IL-7 independent, calling into question the notion that IL-7 selects IL-7Rα+ CD8 T cells into the memory population.

Materials and Methods

Mice, adoptive transfers, and infections

B6.129P-IL-7tm1 mice (IL-7−/−) were obtained from DNAX and backcrossed to C57BL/6J (Ptprcb = CD45.2). Wild-type controls were generated from IL-7−/+ littermates. CD8 T cell responses were monitored following i.v. infection with 105 PFU of vesicular stomatitis virus (VSV). For adoptive transfers, 0.5–1 × 105 B6.SJL-Ptprca Pepcb/BoyJ-Tg(TcraTcrb)1100Mjb/J-B6.129S7-Rag1tm1Mom (CD45.1 OT-I-RAG−/−) CD8 lymph node (LN) T cells were injected into IL7+/−, IL-7−/−, or B6.129S7-Il7rtm1Imx/J mice (IL-7Rα−/−) and infected with 105 PFU rVSV expressing OVA (VSV-OVA).

Tissue isolation and flow cytometry

Lymphocytes were isolated from tissues as previously described (12). VSV-specific CD8 T cells were detected by staining with an H-2Kb tetramer containing the VSVN protein derived peptide for 1 h at room temperature with anti-CD8, CD11a (BD Pharmingen) and IL-7Rα mAbs (eBioscience). For adoptive transfers, cells were stained with Abs specific for CD45.1 (BD Pharmingen), CD8, CD11a, and IL-7Rα, fixed overnight, and stained with an anti-bcl-2 or isotype control mAb (BD Pharmingen). Relative fluorescence intensities were measured using a FACSCalibur (BD Biosciences).

Results and Discussion

IL-7Rα expression following VSV infection

Previous studies demonstrate that following infection with VSV or lymphocytic choriomeningitis virus (LCMV), the majority of Ag-specific effector CD8 T cells down-regulate IL-7Rα (2, 7). In the case of LCMV-specific CD8 T cells, the total number of effector cells which either maintain or rapidly re-express IL-7Rα correlates with the number of memory cells generated (7). To address whether a similar phenomenon occurred following VSV infection, IL-7Rα expression was monitored on responding Ag-specific CD8 T cells (Fig. 1A). Five days postinfection, 21% of the Ag-specific CD8 T cells expressed IL-7Rα and by day 8, 31% of the cells expressed IL-7Rα. Through the contraction phase, the percentage of IL-7Rα+ Ag-specific cells gradually increased, with 91% of the cells IL-7Rαhigh 17 days postinfection. Moreover, a steady-state number of IL-7Rα+ Ag-specific cells was maintained throughout the response (Fig. 1B), suggesting that this population of IL-7Rα+ cells was selected to seed the memory cell pool.

FIGURE 1
The kinetics of IL-7Rα expression on effector CD8 T cells. A, Mice were infected with VSV and the level of IL-7Rα expression on splenic tetramer+ CD8 T cells (solid histogram) was monitored. Values in each panel represent the percentage ...

Studies using mouse lymphocytes and both human and non-human primate PBMC demonstrate that culture with IL-7 dramatically reduces IL-7Rα expression on responding cells, presumably due to the interaction of IL-7 with its cognate receptor (10, 11, 13). However, it is difficult to compare these studies with in vivo analyses because local IL-7 concentrations cannot be accurately measured because serum levels of IL-7 are low (14) and the primary reservoir of IL-7 in vivo is thought to be bound to extracellular matrices (15). To determine whether IL-7 modulated IL-7Rα expression on effector CD8 T cells during a virus infection, we infected IL-7−/− and control IL-7+/− littermates with VSV and monitored IL-7Rα expression on the Ag-specific CD8 T cells in the spleen and lung (Fig. 2). Seven days postinfection, 44 and 20% of CD8 T cells in normal or IL-7−/− mice, respectively, were tetramer+ (Fig. 2A). The splenic response at this time was blunted in the IL-7−/− mice as compared with controls. Interestingly, IL-7Rα down-regulation had occurred on Ag-specific CD8 T cells from both IL-7+/− or IL-7−/− mice (Fig. 2A), with IL-7−/− CD8 T cells expressing somewhat more IL-7Rα than controls. On day 14 after infection, the CD8 T cell response had increased in the IL-7−/− mice from day 7 values, while the response in the normal mice had declined (Fig. 2B). At this time, ~70–90% of the Ag-specific CD8 T cells expressed high levels of IL-7Rα in both control and IL-7−/− mice (Fig. 2B). Forty-four days postinfection, memory cells were present in both normal and IL-7−/− mice (Fig. 2C) and similar levels of IL-7Rα were maintained whether or not IL-7 was present (Fig. 2C). Indeed, as compared with naive CD8 T cells, an increased level of IL-7Rα was noted on memory CD8 T cells even in the absence of IL-7. These results indicated that IL-7 was dispensable not only for down-regulation of IL-7R but that IL-7 was also not required for IL-7Rα re-expression or for selection of IL-7Rα+ CD8 T cells into the memory population. Although TSLP also uses IL-7Rα as a component of its receptor, treatment with an anti-TSLP-R mAb failed to impact IL-7Rα expression kinetics on Ag-specific CD8 T cells in both wild-type and IL-7−/− mice, although the efficacy of such treatment has not been determined (data not shown).

FIGURE 2
IL-7 deficiency fails to impact the proliferation and kinetics of IL-7Rα expression by Ag-specific CD8 T cells. IL-7+/− and IL-7−/− mice were infected with VSV and the frequency of tetramer+ CD8 T cells (left) and their ...

Memory CD8 T cells are generated in IL-7−/− mice

Because the expression pattern of IL-7Rα on Ag-specific CD8 T cells was not altered in IL-7−/− mice, we wished to determine whether memory cells could be generated in these animals. To this end, the total number of IL-7Rα+ effector and memory cells generated in the spleen and lung of IL-7−/− and IL-7+/− mice was calculated (Fig. 3). Although the frequency of Ag-specific CD8 T cells expressing IL-7Rα at the peak of the response was greater in IL-7−/− mice (Fig. 2A), the total number of cells expressing IL-7Rα in IL-7−/− mice was less than in control mice (Fig. 3), perhaps due to the lymphopenic environment of IL-7−/− mice, resulting in a delayed proliferative peak of the CD8 T cell response. Nonetheless, 14 days after infection, the number of Ag-specific CD8 T cells in the lung and spleen was equivalent in both groups and remained similar into the memory phase of the response (day 44). Therefore, both the regulation of IL-7Rα expression on effector CD8 T cells and the generation of the resultant memory cell pool occurred independently of IL-7 signaling. These findings suggested that regulation of IL-7Rα expression was controlled by factors other than IL-7, and that IL-7 did not provide a selective survival advantage to cells expressing IL-7Rα.

FIGURE 3
CD8 T cell memory is generated in IL-7−/− mice. IL-7+/− (solid line) and IL-7−/− (dotted line) mice were infected with VSV and the total number of tetramer+IL-7Rαhigh CD8 T cells was quantitated. Each data ...

IL-7-deficient environment does not alter IL-7Rα expression kinetics and memory cell generation

Because endogenous responding T cells in IL-7−/− animals may be defective in terms of their long-term stability due to a deficiency in homeostatic proliferation (2), we adoptively transferred OT-I TCR CD8 T cells from a normal host into an IL-7 or IL-7Rα null environment. Following infection with VSV-OVA, the kinetics of the proliferative response of the donor OT-I cells in the blood of all recipients was assessed. Although the expansion of the OT-I T cells in the IL-7+/− and IL-7−/− mice was parallel until day 7, from day 11 onward the percentage of OT-I cells was greater in both the IL-7−/− and IL-7Rα−/− mice than in control mice (Fig. 4A). This increased frequency may be due to the lymphopenic environments of IL-7−/− and IL-7Rα−/− mice, although other factors such as increased availability of other growth factors may also play a role. However, when the mice were sacrificed 38 days postinfection, the total number of OT-I memory cells isolated from the spleen of all recipients was similar (Fig. 4B).

FIGURE 4
Regulation of IL-7Rα expression and memory cell generation is IL-7 independent. A, Naive OT-I T cells were transferred into IL-7+/− (■), IL-7−/− (●), or IL-7Rα−/− (▲) mice, ...

We also examined the expression of IL-7Rα by responding OT-I cells (Fig. 4C). Early after infection (day 3), nearly all OT-I cells in either the IL-7+/−, IL-7−/−, or IL-7Rα−/− mice had down-regulated IL-7Rα. By day 5, the peak of the OT-I CD8 T cell response to VSV-OVA, 35–40% of the OT-I cells expressed IL-7Rα irrespective of host type (Fig. 4C). The percentage of IL-7Rα+ OT-I cells gradually increased over time in both groups and by day 38, the OT-I memory cells transferred into both the IL-7+/− and IL-7−/− mice expressed levels of IL-7Rα slightly greater than the level expressed by naive CD8 T cells (Fig. 4C). IL-7 is produced primarily by stromal cells, intestinal epithelial cells, and some subsets of human dendritic cells, it is not produced by T cells (1618), thus, autonomous IL-7 produced by the transferred cells is not responsible for the generation of memory in IL-7−/− mice. Taken together, these data further demonstrated that regulation of IL-7Rα expression and memory CD8 T cell generation occurred in the absence of IL-7.

The proposed selective survival advantage imposed on those effector CD8 T cells maintaining IL-7Rα expression has been linked to the IL-7-mediated induction of the antiapoptotic molecule bcl-2. Indeed, work from our laboratory previously demonstrated that activated OT-I IL-7Rα−/− T cells survived poorly in recipient mice, presumably due to the inability of these cells to express bcl-2 (2). Thus, we decided to assess the status of bcl-2 expression by transferred OT-I T cells in normal and IL-7-deficient environments following VSV-OVA infection. Although naive OT-I T cells expressed bcl-2 directly ex vivo (Fig. 5A), after activation the responding effector cells in both the spleen and lung down-regulated bcl-2 and sustained this low level expression through the contraction phase (Fig. 5B). By day 30 postinfection, although levels had not yet returned to that of the starting population, 25–35% of the responding T cells expressed bcl-2 in both tissues of either the IL-7+/− or IL-7−/− recipients. Thus, because the kinetics of bcl-2 expression in IL-7+/− and IL-7−/− recipients was similar (Fig. 5C), the parallel down-modulation and reacquisition of IL-7Rα/bcl-2 before and during the transition to memory was IL-7 independent.

FIGURE 5
bcl-2 regulation in OT-I CD8 T cells responding to virus infection is not mediated by IL-7. A, Pretransfer, the levels of intracellular bcl-2 (solid histogram) and isotype control mAb staining (open histogram) was assessed on LN-derived OT-I donors. ...

Thus, is IL-7Rα a bona-fide identifier of effector cells destined to enter the memory cell pool? And if so, what molecule(s) other than IL-7 regulate the expression of IL-7Rα by responding effector CD8 T cells? Several studies have analyzed the relationship between IL-7Rα expression and memory cell development with conflicting results. Acute infections with VSV and LCMV induce robust immune responses, preceded by concomitant TCR and IL-7Rα down-regulation (2, 7). Stimulation using anti-CD3 and anti-CD28 in vitro also results in IL-7Rα repression (19). Following acute infection, IL-7Rα is maintained or re-expressed by a subset of CD8 T cells. However, in models of persistent viral infection, Ag-specific CD8 T cells fail to re-express IL-7Rα, likely due to their continual stimulation via the TCR, and remain as IL-7Rαlow“pseudo-effector” T cells (2023). At the other end of the spectrum, weaker Ag stimuli such as peptide-pulsed DCs do not efficiently induce IL-7Rα down-regulation (24). Thus, there appears to be a link between the strength of signal through the TCR, IL-7Rα suppression, and memory development, as opposed to an IL-7-directed survival advantage. It has been shown that as effector cells transition to memory, a distinct program is initiated altering gene expression (25). Perhaps strong TCR stimulation induces this program for memory cell generation and alters IL-7Rα expression, whereas those effectors receiving suboptimal TCR signaling from a weak stimulus or brief Ag encounter fail or inefficiently initiate memory development. An alternate hypothesis is that the level of inflammation affects IL-7Rα expression. This possibility is supported by the findings that either under non-inflammatory priming conditions using either peptide-pulsed DCs (24) or when animals were pretreated with antibiotics before Listeria monocytogenes infection (8), IL-7Rα down-regulation is limited. However, in both cases memory development proceeds normally while in acute inflammatory infections, IL-7Rα is down-regulated and memory is generated (Ref. 7, Fig. 2).

Our data suggested that IL-7 expression was irrelevant for delivery of the appropriate survival signals to effector cells transitioning to memory cells. In addition, dying Ag-specific CD8 T cells express more IL-7Rα than their viable counterparts during the contraction phase of the immune response (24). To account for this discrepancy, it has been suggested that the limited supply of IL-7 in vivo is tightly regulated such that those cells which have received a signal via IL-7Rα down-regulate IL-7Rα, thereby increasing the bioavailability of IL-7 (11). However, our data indicated that engagement of IL-7Rα by IL-7 was not required for receptor down-regulation (Fig. 2 and Fig. 3). Additionally, compensatory molecules involved in long-term survival may exist which are capable of signaling via IL-7Rα or alternate receptors after TCR triggering. Indeed this may be likely because IL-7Rα-deficient CD8 T cells express less bcl-2 following activation and fail to survive long-term, presumably due to intrinsic defects in IL-7Rα signaling (2). However, our results suggested that this was an IL-7-independent phenomenon (Fig. 5). Mice deficient in another γc cytokine, IL-15, generate memory cells, albeit at a lower frequency (26), but with similar IL-7Rα expression kinetics (data not shown). Considering both IL-7 and IL-15 are required for the homeostatic proliferation of memory and memory-phenotype CD8 T cells in lymphopenic environments (3, 4), it is possible that IL-15/IL-15Rα signaling may provide compensatory survival signals in the absence of IL-7. Along similar lines, IL-7−/− mice are lymphopenic, thus perhaps resulting in increased basal levels of IL-15 which may affect memory CD8 T cell development. The lymphopenic environment could also increase the bioavailability of other cytokines such as IL-2 which have been shown to regulate IL-7Rα expression in vitro (19). Alternatively, the absence of a large portion of the lymphocyte pool could also alter the stoichiometry of APCs available to present Ag to the responding CD8 T cells, thus enhancing memory generation in the IL-7−/− mice. Finally, IL-7Rα can form heterodimers with the thymic stromal lymphopoietin receptor (TSLPR) that specifically binds TSLP (27, 28), a cytokine important for CD4 development and homeostasis (29, 30). Signaling through the TSLPR, however, does not appear to influence IL-7Rα expression as in vivo blocking studies using an anti-TSLP-R mAb failed to impact IL-7Rα expression kinetics on Ag-specific CD8 T cells in both wild-type and IL-7−/− mice following infection (data not shown).

Together, our data suggested that stimulation of IL-7Rα with IL-7 is dispensable for the selection and maintenance of memory CD8 T cells and that perhaps stimulation of other receptors, such as the TCR, initially regulates IL-7Rα expression. Therefore, the current paradigm that implicates IL-7 signaling via IL-7Rα as a master regulator of both IL-7Rα expression and memory cell generation requires re-evaluation.

Acknowledgments

We thank Andrew Farr (University of Washington, Seattle, WA) for his generous contribution of the anti-TSLPR mAb.

Footnotes

1This work was supported by National Institutes of Health (NIH) Grants AI41576, DK5260, and AI51583 (to L.L.). K.D.K. was supported by an NIH postdoctoral fellowship (AI053970).

4Abbreviations used in this paper: DC, dendritic cell; VSV, vesicular stomatitis virus; VSV-OVA, VSV expressing OVA; LCMV, lymphocytic choriomeningitis virus; TSLP, thymic stromal lymphopoietin.

Disclosures

The authors have no financial conflict of interest.

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