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A highly stereoselective synthesis of novel cyclically constrained γ-amino acid residues is presented. The key step involves organocatalytic Michael addition of an aldehyde to 1-nitrocyclohexene. After aldehyde reduction, this approach provides optically active β-substituted-δ-nitro alcohols (96–99% e.e.), which can be converted to γ-amino acid amino acid residues with a variety of substituents at the α-position. We have used these new building blocks to prepare α/γ-peptide foldamers that adopt a specific helical conformation in solution and in the solid state.
Oligomers constructed from β-amino acid residues (“β-peptides”) or from combinations of α- and β-amino acid residues (“α/β-peptides”) can adopt protein-like folding patterns.1,2 These conformational properties provide a basis for ongoing development of β- and α/β-peptides that display interesting functional properties. β-Amino acid residues can be endowed with higher intrinsic folding propensities than those of α residues by use of cyclic constraints to limit backbone torsional mobility, and this capacity for residue-based rigidification has proven to be important for both structure and function of β- and α/β-peptide foldamers.3–6 Analogous benefits should result from the use of constrained γ-amino acid residues in foldamers, but it is difficult to explore this hypothesis because only a few types of ring-containing γ-amino acids are known.7,8 The few cyclic γ residues examined to date have been found to promote sheet secondary structure,8b,c which contrasts with the helix-favoring effects of the most common cyclic β residues.1,2,5,6
Here we report a new synthetic approach that provides γ-amino acids containing a cyclohexyl constraint on the Cβ-Cγ bond and a variable side chain at Cα. All three stereocenters of the γ-amino acid skeleton are generated from achiral precursors in a single process with high diastereo- and enantioselectivity. We show that the new type of γ-amino acid residue supports helix formation by an α/γ-peptide backbone.
Figure 1 shows our synthetic approach, the key step of which is the pyrrolidine-catalyzed Michael addition of an aldehyde to 1-nitrocyclohexene. Chiral pyrrolidines have been shown to catalyze the Michael addition of aldehydes to nitroalkenes with high stereoselectivity.9,10 Most precedents involve β-aryl nitroalkenes, such as β-nitrostyrene, which lead to γ2,3-amino acids.10a–d We have reported that Michael addition of aldehydes to nitroethylene provides access to γ2-amino acids.10e Use of pyrrolidine (S)-A along with acidic co-catalyst B proved to be optimal in terms of efficiency and enantioselectivity. Wennemers et al.10f concurrently devised an effective tripeptide catalyst for nitroethylene additions. In complementary work, List et al.10g and Hayashi et al.10h have found that (S)-A catalyzes highly enantioselective Michael additions of acetaldehyde to β-substituted nitroalkenes, which provides γ3-amino acids.
Our attention was drawn to 1-nitrocyclohexene as a Michael acceptor because the adducts could be easily converted to novel cyclically constrained γ-amino acid residues. Reaction of n-butanal and 1-nitrocyclohexene (2:1 molar ratio) in the presence of 20 mol % A in toluene provided only 25% of the Michael adduct after 24 hr, and the two major diastereomers (2a and 3a) were produced in a ~1:1 ratio (Table 1). When 10 mol % B was employed as a co-catalyst, the Michael adduct yield rose to 44% yield, and 2a was favored (6:1 dr); however, the major product was 4, resulting from aldol condensation. Michael adduct yield was improved to 80% (7:1 dr) by using 5 equiv. of n-butanal. Under these conditions, replacing B with either benzoic acid or acetic acid caused a modest decline in diastereoselectivity, and replacing B with trifluoroacetic acid completely inhibited the reaction. We speculate that a key role of the acidic component is to facilitate catalyst turnover, perhaps by promoting hydrolysis of an imminium intermediate. The selectivity for 2a relative to trans diastereomer 3a may result from preferential equatorial protonation of the 2-substituted cyclohexane nitronate intermediate.11
Solvent choice proved to have a substantial impact on Michael adduct yield and diastereoselectivity (Table 2). Both parameters were optimal when the reaction was conducted in CH2Cl2 and catalyzed by 20 mol % A and 10 mol% B, starting with 0.5 M 1-nitrocyclohexene. These conditions led to high selectivity for cis adduct 2a (17:1 dr relative to 3a).
Further exploration revealed that Michael additions catalyzed by A to 1-nitrocyclohexene are highly enantioselective and that many aldehydes are compatible with the catalytic process (Table 3). For these studies, ee was determined by HPLC after γ-nitro aldehydes had been reduced to the corresponding nitro alcohols, to avoid epimerization at the α-carbon. The absolute configuration of the major diastereomer generated with n-butanal and A was determined via derivatization (Scheme 1). Nitro alcohol 5 was oxidized to the corresponding nitro acid 6, which was then coupled to L-phenylalanine methyl ester. The nitro group in the product was hydrogenated, and the resulting amino group was protected with a Boc group. A crystal structure of this α/γ-dipeptide revealed the (S,S,S) configuration for the γ-amino acid residue. The absolute configuration of other Michael adducts (Table 3) was assigned by analogy. γ-Nitro acid 6 could be easily converted to the Boc-protected γ-amino acid 7. In terms of Michael addition scope (Table 3), it is noteworthy that aldehydes bearing a branch point adjacent to nucleophilic carbon (such as 1d and 1h) are tolerated, although these examples require > 2 days to produce good yields, perhaps because steric effects diminish reactivity. The success of the aldehyde with a protected lysine-like side chain (1j) will facilitate the synthesis of oligomers that can be subjected to conformational analysis in aqueous solution.
Overall, the results in Table 3 show that we can gain rapid access to stereochemically pure γ-amino acid building blocks with a cis cyclohexyl constraint in the backbone and a variety of substituents adjacent to the carbonyl. The utility of the Michael addition-based approach is enhanced by the fact that the analogous trans diastereomers can be easily generated as well, as illustrated in Scheme 2. Thus, treating cis nitro alcohol 5 with NaHCO3 in ethanol at reflux quantitatively induces epimerization at the nitro-bearing carbon. Subsequent oxidation yields nitro acid 9, which is identical to the nitro acid obtained by oxidation of 3a (the minor product of the Michael addition, which was characterized crystallographically). Boc-protected γ-amino acid 10 can be readily prepared from 9.
The availability of cyclically constrained γ-amino acid building blocks in stereochemically pure form prompted us to begin to explore the conformational behavior of oligomers containing the corresponding subunits. Recent work suggests that oligomers constructed from α- and flexible γ-amino acid subunits can display a variety of discrete folding patterns.12 We predict that α/γ-peptide foldamers will be conformationally stabilized by γ-residues with appropriate cyclic constraints.
Simulations from Hofmann et al.13 have identified a number of helical conformations that could be adopted by oligomers with a 1:1 alternation of α- and γ-residues. The helix containing 12-atom ring C=O(i)--H-N(i+3) H-bonds, which may be designated the α/γ-peptide “12-helix”, is predicted to have the g, g local conformation about the Cα-Cβ (ζ) and Cβ-Cγ (θ) bonds. Fundamental principles lead one to expect that γ-residues derived from 7 (Figure 2) will favor this local conformation. We hypothesized that the α/γ-peptide 12-helix secondary structure would be favored by combining (R,R,R)-7 (generated with (S)-A) with D-α-amino acid residues. This hypothesis was tested by preparation and analysis of tetramer 11 and hexamer 12.
Crystal structures of both 11 and 12 reveal 12-helical conformations (Figure 3); in each case the maximum number of C=O(i)--H-N(i+3) H-bonds is formed. α/γ-Peptide 12 displayed sufficient proton resonance dispersion in CDCl3 solution to enable NOESY analysis. Among the unambiguous NOEs involving backbone protons, four strong NOEs were observed between protons from different γ-residues: Cγ H(2)--NH(4), Cγ H(2)--Cα H(4), Cγ H(4)--Cα H(6) and Cγ H(4)--NH(6) (Figure 4). The Cγ H(i)--NH(i+2) distances in the crystal structure of 12 are 2.5 and 2.7 Å, and the Cγ H(i)--Cα H(i+2) distances are 2.4 and 2.4 Å, which suggests that these two NOE patterns should be characteristic of the α/γ-peptide 12-helix in solution. Balaram et al.12b have recently suggested that 1:1 α/γ-peptides derived from exclusively achiral amino acids can adopt the 12-helix in chloroform, but in these cases only nearest neighbor NH(i)--NH(i+1) NOEs were observed.
We have developed a short and general route to γ-amino acids that feature a cyclohexyl constraint on the Cβ-Cγ bond and a variety of side chains at Cα. The key step is Michael addition of an aldehyde to 1-nitrocyclohexene, a process that is catalyzed by pyrrolidine A and that strongly favors just one of the eight possible stereoisomers. A second stereoisomer is available via epimerization at Cγ; absolute configuration is controlled by the enantiomer of catalyst A that is employed. α/γ-Peptides containing our constrained γ-residues favor a specific helical conformation. We anticipate that incorporation of these new γ-residues into other types of heterogeneous peptidic backbones will give rise to new families of foldamers, and that synthetic approaches related to those described here will provide access to γ-amino acids with complementary constraints that further broaden the foldamer realm.
This research was supported by NSF (CHE-0848847). NMR spectrometers were purchased with partial support from NIH and NS. We thank Prof. W. Seth Horne and Dr. Soo Hyuk Choi for helpful discussions, Prof. Shannon Stahl and Richard McDonald for assistance with chiral HPLC, and Galina Popova, Andrew Reidenbach, Weicheng Zhang for help with preparation of materials, and Prof. A. J. Andre Cobb for sharing unpublished results.