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A high-sensitivity DNA microarray platform requiring nanograms of RNA input facilitates the application of transcriptome analysis to individual skeletal muscle (SM) tissue samples. Culturing myotubes from SM-biopsies enables investigating transcriptional defects and assaying therapeutic strategies. This study compares the transcriptome of aneurally cultured human SM cells versus that of tissue biopsies.
We used the Illumina expression BeadChips to determine the transcriptomic differences between tissue and cultured SM samples from five individuals. Changes in the expression of several genes were confirmed by QuantiGene Plex assay or reverse transcription real-time PCR. In cultured myotubes compared to the tissue, 1216 genes were regulated: 583 down and 633 up. Gene ontology analysis showed that downregulated genes were mainly associated with cytoplasm, particularly mitochondria, and involved in metabolism and the muscle-system/contraction process. Upregulated genes were predominantly related to cytoplasm, endoplasmic reticulum, and extracellular matrix. The most significantly regulated pathway was mitochondrial dysfunction. Apoptosis genes were also modulated. Among the most downregulated genes detected in this study were genes encoding metabolic proteins AMPD1, PYGM, CPT1B and UCP3, muscle-system proteins TMOD4, MYBPC1, MYOZ1 and XIRP2, the proteolytic CAPN3 and the myogenic regulator MYF6. Coordinated reduced expression of five members of the GIMAP gene family, which form a cluster on chromosome 7, was shown, and the GIMAP4-reduction was validated. Within the most upregulated group were genes encoding senescence/apoptosis-related proteins CDKN1A and KIAA1199 and potential regulatory factors HIF1A, TOP2A and CCDC80.
Cultured muscle cells display reductive metabolic and muscle-system transcriptome adaptations as observed in muscle atrophy and they activate tissue-remodeling and senescence/apoptosis processes.
Oligonucleotide microarrays can reveal gene expression profiles of SM tissue and provide valuable insight into molecular pathways involved in pathogenesis or abnormally regulated in disease. Various human disorders that affect the SM tissue have been analyzed using microarray technology, including disuse atrophy , myositis [2,3], Duchenne muscular dystrophy (DMD) [4-6] and others [7-9].
One of the limitations of applying microarray analyses to such context is the amount of tissue material needed . For SM studies, genome-wide expression profiling has in some cases been applied to pooled samples from multiple patients [4,7]; in others, microarrays with a restricted number of genes were used [2,6,8,9]. The possibility of performing whole transcriptome microarray analysis on small amounts of tissue material facilitates its application to tissue samples from individual subjects.
The culturing of myotubes from SM biopsies opens an alternative to examine transcriptional defects with microarrays and enables therapeutic strategies to be assayed. SM cell cultures can be established by the explant technique , which is based on the presence of myogenic progenitors, the satellite cells . Satellite cells are not committed to fiber type lineages [13,14]. When satellite cells are stimulated with growth factors, they generate myoblasts, which can replicate a limited number of times and be induced to fuse and form multinucleated myotubes. The drawback of cell cultures in general is that cells change their phenotype, which may alter the expression of certain genes and compromise the phenotypic expression of the disease. Indeed, aneurally cultured human SM cells remain relatively immature as shown in studies of the protein isoform pattern [14-16] and microarray analysis of the transcriptome .
There were no data on transcriptome differences between cultured and tissue human SM cells. We deployed an automated high-sensitivity microarray platform to identify genes differentially expressed between aneurally cultured myotubes derived from human SM biopsies by the explant technique and the SM tissue samples. We provide insight into the phenotype of the cultured human SM, which is a valuable cell model for pathogenesis studies and therapeutic assays.
Paravertebral muscles biopsies were obtained from five subjects: females of age 12-15 years devoid of neuromuscular disease during surgery for idiopathic scoliosis. Informed consent and approval from the Ethics Committee of the Hospital Sant Joan de Déu (Barcelona) was obtained. Biopsies were embedded into RNAlater for RNA extraction or into cell culture medium. Cultures were prepared through an explant technique [11,17]. Briefly, SM biopsy pieces were dissected under a stereomicroscope; fat, connective tissue and blood were removed; and the pieces were frozen in DMEM medium with 25% fetal bovine serum (FBS) and 6% DMSO. To set up the culture, first the biopsy pieces were spaced embedded in a semi-solid matrix, composed of 5 ml of DMEM/M-199 medium (3:1) with 37.5% FBS and 1.25 ml of human plasma (Sigma-Aldrich, St. Louis, MO, USA), overspread onto tissue culture plates, which were then incubated for 6 to 8 days to permit fibroblast outgrowth. The biopsy pieces were then removed from the matrix with forceps under the stereomicroscope, dissected and embedded in a matrix, composed of 1 ml of human plasma and 2 ml of 1.5% gelatin, spotted and stuck onto new dishes and overlaid with DMEM/M-199 medium (3:1) with 10% FBS, 10 μg/ml insulin, 4 mM glutamine, 25 ng/ml fibroblast growth factor, and 10 ng/ml epidermal growth factor, for 5 to 7 days to permit myoblast proliferation and migration. After myoblast generation, the biopsy pieces were removed, and eventually re-explanted, and myoblast monolayers were dissociated with trypsin and subcultured. Myogenic cells from the same explant were subjected to limited consecutive subcultures to avoid differences resulting from senescence . Myotubes were derived from confluent myoblast cultures; immediately after initiation of myoblast fusion, medium was replaced by DMEM/M-199 medium (3:1) with 10% FBS and 10 μg/ml insulin, to further stimulate differentiation. Myotubes were used 7 days later. Myotube preparations were labeled B19, B22, B24, B25 and B26.
Cells grown on coverslips were fixed in 4% paraformaldehyde in PBS for 15 min, then washed in PBS, and incubated for 10 min in PBS containing 50 mM NH4Cl, 10 min in PBS containing 20 mM glycine, 10 min in PBS containing 0.1% Triton X-100 and 30 min in PBS containing 10% FBS. Subsequently coverslips were incubated for 1 h at room temperature with a rabbit anti-desmin antibody (1:100; AB907 Millipore, Billerica, MA, USA). Primary antibody was detected with an Alexa Fluor-488 goat anti-rabbit antibody (1:500; Molecular Probes, Eugene, OR, USA). Both primary and secondary antibodies were diluted in blocking solution. Nuclei were stained with Hoescht (1 μg/ml) during secondary antibody incubation. After staining, samples were mounted on Mowiol mounting medium and analyzed with a Leica TCS SP2 confocal microscope. Images were processed using Photoshop CS software (Adobe Corp, San Jose, CA, USA). From 200 to 500 nuclei in each of the 5 myotube cultures were analyzed with ImageJ (Rasband WS, ImageJ, National Institutes of Health, Bethesda, MD, USA, http://rsb.info.nih.gov/ij/ and the mean ± SD values of the percentage of nuclei located in desmin-labeled myotubes were calculated.
Ten samples, 5 from cultured SM cells, and 5 from SM biopsies were analyzed by DNA microarrays. Total RNA was extracted from SM biopsies with RNeasy fibrous tissue kit and homogenized using a TissueLyser, and from cultured myotubes with RNeasy kit (Qiagen, Valencia, CA, USA), with the same DNase treatment than in the RNeasy fibrous tissue kit, and homogenized with a Polytron. RNA samples were quantified with the RiboGreen RNA Quantification Kit (Molecular Probes), and monitored with the Agilent 2100 Bioanalyzer, to check high-quality RNA (RNA integrity = 8). We used HumanRef-8 v2.0 Expression BeadChips (Illumina, San Diego, CA, USA), which comprise probes to interrogate 22200 transcripts, based on the curated content of the NCBI Reference Sequence database, release 17.
An aliquot of 150 ng of total RNA was used to produce double-stranded cDNA, followed by transcription in vitro, and by cRNA labeling with biotin using the TotalPrep RNA Amplification kit (Applied Biosystems/Ambion, Austin, TX, USA) recommended by Illumina. This method is based on the protocol developed in J. Eberwine's laboratory . The procedure consists of reverse transcription with an oligo(dT) primer bearing a T7 promoter using a reverse transcriptase engineered to produce high yields of first strand cDNA. The reverse transcriptase catalyzes the synthesis of full-length cDNA which then undergoes second strand synthesis and clean-up to become a template for in vitro transcription (IVT) with T7 RNA Polymerase. The IVT, along with biotin UTP, is used to generate hundreds to thousands of biotinylated, antisense RNA copies of each mRNA. These procedures were performed on an automated system primarily developed for the preparation of samples for Affymetrix arrays , which we adapted for the Illumina procedure. It is composed by a Microlab Star liquid handling system (Hamilton, Bonaduz, Switzerland) for reagent and sample pipetting and mixing, coupled with a thermocycler TRobot (Biometra, Goettingen, Germany) for incubations, and a microtiter plate reader SpectraMax M2 (Molecular Devices, Sunnyvale, CA, USA) for nucleic acids quantification with RiboGreen assay. The 10 samples analyzed in the present experiment were processed in a single robotic session. The labeled-cRNAs thus produced were measured with the Agilent 2100 Bioanalyzer to control for length. All cRNA sizes were close to 1200 nt and therefore eligible for hybridization onto the microarrays. Then, 750 ng of labeled-cRNAs was added to the hybridization mix, which contained control oligonucleotides in hybridization buffer. Then, 15 μl of each hybridization mix was dispensed on the BeadArrays. After hybridization (16 h, 58°C), the arrays were washed to remove non-hybridized material and were stained with streptavidin-Cy3, which bound to biotin. Scanning was performed using the BeadArray Reader, which provided intensity values for all transcripts. Bead redundancy allowed the calculation of a detection p-value which served at declaring the transcripts "significantly detected" or not. Signal intensities were extracted and summarized in the BeadStudio software. Data were expressed as absolute intensities, to which we applied a background correction. Background was calculated for each array by the average signal of the negative control probes. All microarray experiments were run at the same time. The Illumina HumanRef-8 BeadChip is a glass slide comprising 8 identical microarrays. We therefore used 2 slides for this experiment. To minimize possible batch effect that can occur between the two glass slides, we randomized the sample distribution in the following order: in one slide A (B19 in vitro), B (B26 in vitro), C (B25 in vivo), D (B24 in vitro), E (B24 in vivo), F (B22 in vivo), G (B26 in vivo) and H (B25 in vitro) and in the other slide A (B19 in vivo) and B (B22 in vitro). "In vitro" stands for cultured myotubes and "in vivo" means SM tissue biopsies. As shown in a recent study , randomization on Illumina slides greatly improves the results reducing the number of possible false positive occurrences that would lead to a misinterpretation of the data. Microarray data files have been deposited in GEO Omnibus [http://www.ncbi.nlm.nih.gov/geo/ GEO accession number GSE17503].
QuantiGene Plex 2.0 assay (Panomics/Affymetrix, Fremont, CA, USA) was used for confirmation of the microarray analysis. The advantage of this assay is that no RNA amplification is needed, unlike microarrays or quantitative PCR. A comparison to other gene expression platforms in the frame of the MAQC project demonstrated the sensitivity and accuracy of QuantiGene techniques . This technology uses three sets of pooled oligonucleotides that function to capture, provide binding sites for signal amplification molecules, and stabilize the mRNA of interest. The capture extender (CE) pool contains oligonucleotides that are complementary to the mRNA of interest and to capture probes (CPs) covalently linked to carboxylated fluorescent-encoded micro spheres (Luminex, Austin, TX, USA). The label extender (LE) pool has oligonucleotides that contain sequences complementary to the mRNA of interest and to the amplifier molecules which carry a streptavidin phycoerythrin (SAPE) conjugate. The blocker (BL) pool contains oligonucleotides that are complementary to the mRNA where neither CE nor LE bind and it protects the mRNA by maintaining an intact RNA-DNA hybrid. The micro spheres (or beads) thus prepared are analyzed with a Luminex reader, which utilizes two lasers, the first for bead identification, measuring the fluorescent signal specific to each bead type, and the second for mRNA quantification, determining the fluorescence level of SAPE and therefore the expression level of the transcript. The assay was performed according to the manufacturer's instructions, accessible at: http://panomics.com/downloads/UM13075_RevB_QGP2_080430.pdf. Briefly, the RNAs extracted from tissue and cell samples were deposited on a 96-well plate along with the QuantiGene hybridization solution (containing RNase-free water, lysis mixture, blocking reagent, proteinase K, capture beads and probe set). The hybridization plate was then sealed and placed in a shaking incubator (Vortemp 56 Shaker/Incubator, Labnet International, Inc., Woodbridge, NJ, USA) at 54°C and 600 rpm for overnight hybridization (18-22 h). After centrifugation to remove condensation, the hybridization mixture was transferred to a pre-wetted filter plate, where the mixture was filtered and washed three times in wash buffer. All washing steps were performed with a MultiScreen Vacuum Manifold (Millipore) at low pressure, and after the final wash, the filter plate bottom was blotted to remove any remaining liquid to prevent leakage. The samples were then incubated successively in three working solutions: pre-amplifier and amplifier, biotinylated probes and SAPE. Two washes were performed between each incubation to remove unbound reagents. The plate was covered with aluminum foil during SAPE incubation to prevent photo bleaching. For Luminex measurement, after a calibration of the Luminex, the plate was placed in the instrument. Prior to the measurement, each bead type was identified in the Luminex software in order to specify to which transcripts the beads corresponded. At the start of the measurement, the content of each well was aspirated and pushed towards the lasers. As occurs in a flow cytometer, the beads passed successively in front of the two lasers. Beads were identified, and fluorescent signals were measured. The final outcome was a raw data file containing signal intensity and background level for each gene in each sample. Data were analyzed using the transferrin receptor (TFRC) gene as the reference gene, since its expression was similar within the two examined groups, cultured myotubes and SM biopsies.
For reverse transcription (RT) and real-time PCR, an aliquot of 0.5 μg of total RNA was retro-transcribed (RT) with TaqMan reverse transcription reagents from Applied Biosystems (Foster City, CA, USA) using random hexamers and in a 25 μl total volume reaction. Real-time PCR was performed on 1 μl of the RT reaction mixture with the TaqMan universal PCR master mix and TaqMan Gene Expression Assays using the ABI PRISM 7700 sequence detection system. Probes for PYGM (Hs00194493_m1), UCP3 (Hs00243297_m1), THY1 (Hs00264235_s1) and B2M (Hs99999907_m1) were from Applied Biosystems. The threshold cycle (Ct) values of the reference probe beta-2 microglobulin (B2M) were subtracted from each target probe Ct values (ΔCt), the ΔCt values of the SM biopsies were subtracted from the ΔCt values of myotube cultures. Data were expressed as mean values of 2-ΔΔCT for upregulated genes and 1/2-ΔΔCT for downregulated ones and the significance of differences was estimated by a paired t-test.
After the scanning of the microarrays, BeadStudio generated a dendrogram to control for the sample clustering. Subsequent steps of quality control (QC), statistical analysis and filtering of microarray data were carried out with GeneSpring GX software v10.0.2 (Agilent Technologies, Santa Clara, CA, USA). Raw data with background subtracted were loaded from Illumina BeadStudio to GeneSpring GX. This step was followed by quantile normalization and log2 transformation. The samples were then grouped into two conditions which we called "in vitro" and "in vivo", corresponding respectively to the SM cultures and the tissue biopsies. GeneSpring GX was used as the first step for QC of data on all the samples with two unsupervised methods, Pearson correlation and Principal Component Analysis (PCA). The PCA vector space transformation was used to reduce multidimensional data sets to lower dimensions for analysis to assess the behavior of the samples and to identify or confirm possible outliers. Within the quality control step, Genespring GX enables to compute the 3 first principal components. The percentage of total variance that each of these 3 principal components capture is shown in the legend of Additional File 1.
To assess which genes were differentially expressed between the two conditions examined, we performed a corrected paired t-test on all probes. Because of the small number of biological replicates, we used the Benjamini-Hochberg false discovery rate (FDR) method controlling for false positives, in order to select genes with the lowest FDR. This method is less stringent than other corrections such as the Bonferroni, and provides a good balance between discovery of statistically significant genes and limitation of false positive occurrences. A corrected p-value cutoff of 0.01 was used to select the regulated genes with the lowest FDR. A fold change value was also computed by GeneSpring GX to assess the level and the direction of the gene regulation. Fold change was calculated as the absolute ratio of normalized intensities between the mean values of the samples in the two conditions, cultured myotubes and SM tissue biopsy, grouped.
Data from QuantiGene Plex were also loaded into GeneSpring GX software. Originally GeneSpring GX was designed for gene expression microarray experiments and uses the main commercially available arrays (Affymetrix, Illumina, Agilent and CodeLink). However, it is possible to configure a "custom" array. The QuantiGene Plex assay was therefore configured in GeneSpring GX. After normalization to the TFRC control gene, data were log2 transformed, and significance of differential expression was assessed by a paired t-test.
Functional annotation and classification analysis was also carried out with GeneSpring GX program, running a Gene Ontology (GO) analysis on the gene set selected with the statistical analysis described above. GeneSpring GX computed a p-value to quantify the significance in the GO analysis. This p-value is the probability that a random subset of x genes drawn from the total set of n genes will have y or more entities containing a given GO term. This probability is described by a standard hypergeometric distribution. GeneSpring GX uses the hypergeometric formula from first principles to compute this probability. Since a large number of hypotheses will be tested, some form of correction is required. GeneSpring GX addresses this issue using the Benjamini-Yekutieli correction, which takes into account the dependency among the GO terms. In the GO analysis done in GeneSpring GX, a low p-value therefore implies that the given GO term is enriched. The Gene Ontology data in GX 10.0.2 is compiled from version 1.2 of OBO (Open Biomedical Ontologies).
Data were interpreted using Ingenuity Pathways Analysis (IPA) (Ingenuity Systems, Redwood City, CA, USA) http://www.ingenuity.com. The list of significantly regulated genes selected by the microarray analysis described above was loaded in IPA with the following criteria: Reference set: HumanRef-8 v2.0; Direct and Indirect relationships included; filtered by species (human), and in a second round, by tissue (skeletal muscle). Then IPA computed the data to generate significant networks of genes that are associated with particular biological functions, diseases, and molecular processes. It showed also the canonical pathways stimulated by the experiment. IPA used the right-tailed Fisher's exact test to extract significant pathways. IPA canonical pathways are well-characterized metabolic and cell-signaling pathways coming from articles, reviews, books, and KEGG Ligand.
Gene cluster analysis was performed with the REEF (REgionally Enriched Features in genomes) program, which scans the genome using a sliding window approach, and calculates the statistical significance of each window using the hypergeometric distribution and the false discovery rate .
Microarray analyses were performed on the 5 SM cultures and the 5 SM biopsies. We confirmed that myotubes were the prevalent cell type in each of the SM cultures by immunostaining for desmin, a muscle-specific intermediate filament protein  (Additional file 1, Figure S1). Most of the Hoescht-stained nuclei were located in desmin-labeled myotubes: B19 (78.3 ± 3.12%), B22 (61.4 ± 6.60%), B24 (61.8 ± 8.36%), B25 (83.6 ± 14.50%) and B26 (79.7 ± 5.05%). The microarray analysis showed that in the cultured myotubes compared to the tissue biopsies 1260 transcripts, which correspond to 1216 nuclear genes, were differentially expressed (selection criteria: absolute fold-change value > 2 with a corrected p-value of p < 0.01). Of these, 583 were downregulated and 633 were upregulated. The complete list of regulated genes is shown in Additional file 2, Table S1. On the basis of the correlation analysis performed, Additional file 3, Figure S2 shows that the samples were clearly separated based on their condition cultured myotubes or SM biopsy. The dendrogram also illustrates that the paired samples were differently distributed in the two conditions. PCA and correlation matrix procedures show that the two experimental conditions were well separated and there were no particular outliers in the experiment. Moreover, they highlight that the variability was higher within the SM culture group (in vitro). Pearson coefficients are also provided; the average Pearson coefficient was 0.97 for the SM biopsies and 0.93 for the SM cultures. PCA, correlation matrix and dendrogram also confirmed that there was no significant batch effect between the two Illumina slides in our experiment. In an attempt to restrict differentially expressed genes to those expressed in SM, microarray data were filtered according to the IPA knowledge-base, so that genes expressed in SM were selected. SM-specificity is shown in Additional file 2, Table S1. IPA knowledge about mRNA expression in tissues is based on two sources: the GNF body atlas , and literature findings. Among the downregulated genes, 384 passed this filter along with 318 of the upregulated genes. The filtering served as a means for identifying genes that are expressed in other cell types, present in the SM tissue, within the most regulated genes. For instance, hemoglobin HBB and HBA2 genes, which are mainly expressed in erythroid cells , from the most downregulated group and the THY1 gene, which is mainly expressed in human fibroblasts, neurons and endothelial cells , from the most upregulated group. Remarkably, the ratios between the normalized signal of the THY1 and desmin gene markers in the SM cultures were in a narrow range: B19 (1.94), B22 (1.12), B24 (1.42), B25 (1.28) and B26 (1.44), again indicating that fibroblast presence did not vary widely within SM cultures. However, the THY1/desmin ratios were from 1.3- to 3.2-fold higher in the SM cultures than in the SM biopsies.
The complete set of differentially expressed genes were classified, separately for downregulated and upregulated genes, according to GO (Additional file 4, Table S2A). With respect to the cellular component, genes that were downregulated in cultured myotubes were mainly associated with cytoplasm and enriched in mitochondria. The over-represented biological processes were metabolism, with the largest group of regulated genes, and muscle-system/contraction. With respect to metabolism, genes were mainly involved in the generation of precursor metabolites and energy, cellular respiration and oxidative phosphorylation; quinone cofactor metabolism was also affected. The most augmented molecular function was oxidoreductase activity. Among the genes that were upregulated in cultured myotubes, the enriched cellular components were cytoplasm (with the largest group associated with endoplasmic reticulum (ER)) and the extracellular matrix. The enriched molecular function here was oligosaccharyl transferase activity. The augmented biological process was modification of an amino acid residue in a protein: N-linked posttranslational glycosylation at asparagine residues. GO annotations of the SM-expressed subset of downregulated genes (Additional file 4, Table S2B) did not differ greatly from those of the whole gene set, except for the occurrence of the following over-represented GO annotations: cytoskeletal protein binding (molecular function); fatty acid beta-oxidation and organic acid metabolism-related terms (metabolic processes); and muscle development (biological process). There was no GO annotation enrichment within the upregulated SM-expressed subset for molecular function and biological process categories (Additional file 4, Table S2B).
The most regulated SM-expressed genes on the basis of the fold-change are listed in Table Table1.1. This selection was performed on the whole set of 1260 regulated transcripts (data not shown) before the replicated genes were removed. In Additional file 5, Table S3, the standard deviation for each of these genes, based on the normalized signals, is shown. A closer examination of the downregulated group reveals several muscle-system genes encoding proteins of myofilaments MYL3; associated/regulatory proteins TMOD4, MYBPC1, MYOZ3, MYOZ1, NRAP and XIRP2; the regulator of the muscle sarcomere CAPN3 ; and calcium-related proteins CASQ1 and S100A1. Genes encoding metabolic proteins are included in this group: LPL, CPT1B, PYGM and AMPD1. Potential regulatory factors are also present: members of the ankyrin repeat- and SOCS (suppressor of cytokine signaling) box-containing protein (ASB) family, ASB10 and ASB12; members of the muscle ankyrin repeat protein (MARP) family, ANKRD2 and ANKRD23; and the transcription factor MYF6. The ion channels CLIC5 and KCNA7, the transporters AQP4 and MB, and the neurite outgrowth inhibitor RTN4 are included. Other genes such as those encoding NIPSNAP3B, HHATL and MYOC have no defined function in SM.
Among the most upregulated genes detected in this study (Table (Table1)1) are well-known regulatory factors such as CDKN1A/p21, TOP2A and HIF1A. Several genes are related to the extracellular matrix, such as those encoding: the Ca(2+)-binding proteoglycan SPOCK1; isoforms of lysyl hydroxylase (PLOD3, PLOD2) , an enzyme that catalyzes the formation of hydroxylysine in collagens; CSGlcA-T, which is involved in the synthesis of chondroitin sulphate as a glucuronyltransferase ; the metalloproteinase MMP14; TIMP2, an inhibitor of the metalloproteinase type IV collagenolytic activity  and the protease inhibitor SERPINE2 . The NFASC gene, which promotes axon subcellular targeting and synapse formation , was another highly induced gene, suggesting stimulation of neuronal signaling. Two genes are related to stress: the gene encoding the putative glutathione peroxidase GPX8, an enzyme involved in protection against oxidative stress , and the DNAJC10/ERDJ5 gene. The PTX3 gene is related to inflammation . Other genes are related to sphingomyelin metabolism such as those encoding: GBA, a lysosomal enzyme that catalyzes glucosylceramide breakdown , and SGMS2, an enzyme that produces sphingomyelin . Finally, a group of genes encodes proteins that either have an undefined (i.e. TMEM200A), or an unknown function in the SM tissue (KIAA1199, PSD3, CCDC80/URB/SSG1/DRO1 and LPIN2).
We assessed the changed expression for 17 genes that were selected as follows. Within the 10% most regulated genes, including both downregulated and upregulated groups, we selected genes that were involved in various regulated biological functions (metabolism, muscle-system/contraction and apoptosis), the GIMAP4 family member, the transcription factors MYF6 and HIF1A and other potential regulators such as TOP2A and CCDC80, and THY1. We used the QuantiGene Plex assay to corroborate most of the genes. This assay does not require any RNA amplification, unlike microarrays or quantitative PCR, and is both sensitive and accurate . However, 3 of these genes were validated by the customary, readily accessible RT and real-time PCR technique . Differential expression was confirmed for all of the selected genes (Table (Table2).2). Differences in downregulated genes that were assessed with the QuantiGene Plex assay were equivalent to or greater (less than 6-fold) than those assessed with the microarray, as were the differences assessed with RT and real-time PCR (12- or 20-fold greater). In the latter case, increased expression of the B2M control gene in SM cultures compared to SM tissue (Additional file 2, Table S1), which was confirmed by RT real-time PCR, may have contributed to increasing the differences. In contrast, differences in upregulated genes measured with the QuantiGene Plex assay were smaller than those measured with the microarray (1.7- to 7-fold). However, with THY1, the difference assessed by RT and real-time PCR was similar.
Since members of the same gene family or genes that are coordinately expressed might form clusters on chromosomes, we tested the possibility that coregulated genes showed spatial clustering. We applied the GeneSpring GX program to the entire set of 1216 differentially expressed genes to identify the chromosome and genomic position. Of these genes, 983 had chromosome annotations that were distributed throughout all chromosomes (Figure (Figure1A),1A), although the highest number was on chromosome 1. We applied the REEF program to the whole set of 983 differentially expressed chromosome-annotated genes to search for local clustering. Three gene clusters were recognized using the REEF program (Figure (Figure1B).1B). The cluster on chromosome 7 (Figure (Figure1C)1C) contained the largest number of genes and included five members of the GTPase of the immunity-associated protein (GIMAP) gene family, plus ASB10 and CSGlcA-T. Except the CSGlcA-T gene, all genes in the cluster are highly downregulated. The genes encoding GIMAP4, GIMAP5, ASB10 and CSGlcA-T are expressed in SM (Additional file 2, Table S1). No gene families were detected in clusters on chromosomes 1 and 20.
To reveal regulated metabolic and signaling pathways the complete set of differentially expressed genes was analyzed by IPA. According to IPA, 10 signaling and 13 metabolic canonical pathways are regulated in myotubes versus SM tissue (Table (Table3).3). The most regulated metabolic pathways are related to mitochondria: the citrate cycle, oxidative phosphorylation and ubiquinone biosynthesis. Consequently, mitochondrial dysfunction is the most regulated signaling pathway. Network analysis of this pathway illustrates known interactions between the identified genes and overall pathway downregulation (Figure (Figure2).2). Included genes are those encoding: components of the oxidative phosphorylation system (most of which are in complex I, some are components of complexes II, III and IV and cytochrome c CYCS); UCP3, an inner mitochondrial membrane transporter that dissipates the proton gradient ; CPT1B, which is associated with the outer mitochondrial membrane and facilitates the mitochondrial import of long chain fatty acids, and ACACB, which converts acetylCoA into malonylCoA to inhibit CPT1B ; MAOB, an enzyme involved in the degradation of biogenic amines ; the pyruvate dehydrogenase E1-alpha subunit (PDHA); and alpha-ketoglutarate dehydrogenase (KGDH). All these genes are expressed in SM except the NDUFAB1, NDUFA4, COX10 and UQCRB genes according to the IPA knowledge-base. Additional regulated metabolic pathways involve glucose, amino acid and monocarboxylic acid metabolism. Results were similar when the subset of SM-expressed genes was analyzed. However, the pathways One carbon pool by folate and Biosynthesis of steroids were not enriched, whereas the pathways Fatty acid metabolism, Phenylalanine metabolism and Glyoxylate, dicarboxylate metabolism were enriched with -log(p-values) of 2.38, 2.13 and 1.81, respectively.
Other regulated signaling pathways in the complete set of the differentially expressed genes relate to cell interaction with the extracellular matrix (integrin signaling, hepatic fibrosis), cell communication with its environment (caveolar-mediated endocytosis) and actin-cytoskeleton signaling. In addition, circadian rhythm signaling was altered, with five genes all downregulated. Finally, apoptosis and the signaling of phosphatase and tensin homolog (PTEN) (Figure (Figure3)3) were regulated. PTEN dephosphorylates the signaling lipid phosphatidylinositol (3,4,5)-trisphosphate and affects cellular processes related to cell proliferation, apoptosis and muscle contractility . All signaling pathways except the p53-pathway were significantly and similarly regulated when the subset of SM-expressed genes was analyzed (data not shown).
Using the high-sensitivity Illumina microarray platform, we identified changes in the human SM cell transcriptome that were induced by tissue biopsy culture based on the explant technique. More than one thousand genes displayed differential expression between cultured myotubes and SM tissue. A similar number of genes were upregulated and downregulated: 65% and 50% of the genes in each group, respectively, were expressed in SM according to the IPA knowledge-base.
Ontology analysis of downregulated genes in SM cultures revealed the following. In terms of their associated cellular components, there was an over-representation of genes encoding proteins located in mitochondria. In terms of their associated cell process, the genes that were enriched were involved in energy metabolism and the muscle-system. In line with this, the genes that had the most reduced expression included those encoding key regulators of glycogenolysis (PYGM), fatty acid oxidation (CPT1B) and preservation of ATP formation (AMPD1). Various genes involved in glycolysis, energy consumption (UCP3) and oxidative phosphorylation (complexes I, II, III and IV) were also repressed. IPA pathway analysis consistently revealed that the majority of regulated metabolic genes are involved in mitochondrial pathways of energy production and the most regulated signaling pathway is mitochondrial dysfunction. The highly downregulated genes also included those that encode proteins associated with myofilaments, calcium-binding proteins and CAPN3, a muscle-specific calcium-activated nonlysosomal cysteine protease that contributes to sarcomere homeostasis . Downregulated genes are slow-type isoforms such as MYL3, MYBPC1 and MYOZ1 and isoforms that are predominantly expressed in fast-type muscle fibers such as TMOD4, CASQ1 and MYOZ3. Noteworthy, the lumbar paravertebral muscles in humans are described as containing fiber types, type I (slow) and type II (fast), in similar proportions [41,42]. The downregulation found in the metabolic and muscle-system profile in SM cultures is consistent with findings in several myopathies. For instance, energy metabolism genes were the major group of genes downregulated in human muscle by disuse atrophy , DMD [6,7], α-sarcoglycan deficiency  and X-linked myotubular myopathy (XLMTM) . Decreased expression of genes encoding muscle-system/contraction associated or regulatory proteins was observed in DMD  and XLMTM patients . In muscles of immobilized legs in patients , major categories of downregulated genes were involved in sarcomere structure and protein turnover (including the CAPN3 gene). According to our data, the full phenotype of several muscle pathologies may not be expressed in cultured muscle as the genes are markedly downregulated. This includes PYGM-deficiency (McArdle disease) ; lack of AMPD1, which causes metabolic myopathy in humans ; and loss-of-function mutations in the CAPN3 gene that have been associated with limb-girdle muscular dystrophy type 2A (LGMD2A) .
We identified potential regulators of SM function among the most downregulated genes. Myf6/MRF4 is a member of the family of myogenic regulatory factors that includes MyoD, Myf5 and myogenin . MRF4 expression is sufficient for conversion of fibroblasts to myogenic lineage . However, MRF4 did not stimulate myogenic differentiation in C2C12 cells, but cell proliferation, in contrast to MyoD . In addition, it was inefficient at trans-activating contractile protein genes, unlike MyoD and myogenin . Various genes encoding proteins that contain the ankyrin repeat structural motif were strongly downregulated, namely, the ASB family members ASB10 and ASB12. ASBs are implicated in various biological processes. For example, ASB15 promotes muscle cell growth and stimulates protein synthesis , while ASB6 regulates components of the insulin signaling pathway in adipocytes . Two members of the MARP family are also included: ANKRD2 and ANKRD23. ANKRD2/Arpp appears to be involved in sensing stress signals and linking these to muscle gene regulation , and ANKRD23/DARP is upregulated in insulin-resistant animals . Abrogation of the three MARP family members CARP, ANKRD2 and DARP in mice indicated a role in passive mechanical behavior and in the regenerative response in muscle .
In myotubes versus SM tissue, we found coordinated reduced expression of five members of the GIMAP gene family GIMAP1, GIMAP4, GIMAP5, GIMAP6 and GIMAP7, which form a cluster on chromosome 7 and participate in cell survival/death. Seven GIMAP genes are functional: GIMAP1, GIMAP2, GIMAP4, GIMAP5, GIMAP6, GIMAP7 and GIMAP8 . The GIMAPS are also called immune-associated nucleotide-binding proteins and are considered cell survival regulators, with a crucial role in lymphocytes: GIMAP5 is a key genetic factor for lymphopenia in spontaneous BioBreeding rat insulin-dependent diabetes and GIMAP4 has been shown to accelerate programmed cell death in T-cells . In humans, the GIMAP family genes are expressed in several tissues , although the highest expression of most of these genes is in immune tissues. However, to the best of our knowledge, this is the first description of coordinate regulation of the expression of the genes in this family, which indicates the participation of common mechanisms of transcriptional regulation: shared control regions or the same control elements in each gene.
In contrast, most of the upregulated genes in culture were located in the ER and extracellular matrix on the basis of the gene ontology analysis. Induced genes included those involved in tissue remodeling, such as genes encoding TIMP2, PLOD2 and PLOD3, CSGlcAT, SERPINE2 and MMP14, all of which were among the 10% most regulated. In addition, induced genes included those involved in the formation of the extracellular matrix, such as those encoding collagens, laminins, fibronectin, integrins and proteoglycans. This increase may be partially due to the enrichment of SM cultures in fibroblasts compared to the tissue. This is indicated by the increased expression, albeit less than that of the 10% most regulated, of the THY1 gene, which is expressed in fibroblasts, neurons and endothelial cells. Myotubes may also contribute to this increase in expression. In this regard, IPA analysis revealed alteration of signaling pathways related to cell interaction with the extracellular matrix/environment in either the whole set or the subset of SM-filtered differentially expressed genes. Remarkably, the proteoglycan SPOCK1/SPARC/OSTEONECTIN gene was highly induced. Upregulation of genes of the extracellular matrix are common findings in muscle from DMD [5-7], LGMD2A  and XLMTM patients , where it is considered to reflect dystrophic changes. In some cases, the SPARC gene is also included [5-7,9]. In our SM cultures, the strong induction of DNAJC10/ERDJ5 gene expression suggests ER stress. DNAJC10/ERDJ5 is abundant in secretory cells and induced during ER stress . It resides in ER, where secreted proteins are translocated and encounter the folding machinery; it has disulfide reductase activity and is involved in the retro-translocation of misfolded proteins into cytosol for degradation .
One of the most induced genes in culture is that encoding CDKN1A/p21, an inhibitor of cell cycle progression, involved in the p53-dependent cellular senescence, possibly mediating apoptosis execution . Another highly induced gene encodes KIAA1199, a protein of undefined function that was identified as an induced transcript in replicative senescent-induced renal carcinoma cells by transfer of human chromosome 3 , and as a candidate for hearing loss . These data suggest that cultured muscle cells have activated a senescence process that was not established in the muscle tissue. Accordingly, the IPA analysis detected apoptosis as a regulated signaling pathway.
Among the most induced genes in SM cultures were potential transcriptional regulators. These included the gene encoding TOP2A, a DNA topoisomerase that is associated with the RNA polymerase II holoenzyme and is a required component of chromatin-dependent coactivation , and the HIF1A/MOP1 gene, which encodes the oxygen-sensitive alpha subunit of the transcription factor hypoxia inducible factor-1 (HIF-1) that forms a heterodimer with the beta subunit . During hypoxia the HIF1A protein is stabilized and activated. The HIF1A gene is expressed constitutively in some cell types, in others it is upregulated by hypoxia  or obesity , although downregulation of mRNA levels by hypoxia has also been reported in human fat cells . With respect to hypoxia, we found that the MB gene encoding the oxygen-binding myoglobin is one of the most repressed in cultured SM cells. Among the targets of HIF-1 , several genes showed increased expression in the SM cultures, such as the gene encoding the protein related to vascular tone, ADM; to angiogenesis, VEGFC; to glucose metabolism, ENO1; and the homolog of the pro-apoptotic BNIP3 protein BNIP3L/NIX gene, which suggests increased activity of the HIF-1 transcription factor. However, other HIF-1 gene targets involved in glucose metabolism  were not upregulated. The overexpression of HIF1A factor is related to local invasion and metastatic spread of tumor cells . Thus, in SM cultures it may contribute to the activation of the tissue remodeling process. Moreover, the HIF-1 factor has been related  to resistance to anoikis, a programmed cell death induced by the loss of or inappropriate cell adhesion, in transformed epithelial cells and it might also have this role in the SM cultures.
Two genes with potential involvement but no clear role in metabolic control also showed increased expression in culture. One is the gene encoding CCDC80/URB/SSG1/DRO1, which was identified as an upregulated transcript in the adipose tissue of bombesin receptor subtype-3 (BRS-3)-deficient mice displaying mild late-onset obesity  and sensitizes cells to anoikis and apoptosis . The other is the gene encoding LPIN2, which is a member of the lipin protein family that possesses phosphatidate phosphatase activity converting phosphatidate to diacylglycerol. The function of the latter has not yet been defined and mutations of the gene have been found in patients with Majeed syndrome, an auto-inflammatory disorder .
In summary, our data show a reduction in the metabolic and muscle-system transcriptome in the SM cultures that may be due to inappropriate stimuli, including the lack of innervation, the limited extracellular matrix molecules in SM cultures or other less-defined stimuli such as circadian rhythm. The fact that the tissue remodeling transcriptome is increased and signaling pathways related to cell interaction with the extracellular matrix/environment are regulated indicates that a lack of appropriate matrix stimuli may be involved, at least partly. The hypoxia response pathway, which appears to be activated in SM cultures, may to some extent trigger the tissue remodeling process. Finally, the atrophic phenotype of cultured myotubes is associated with the induction of genes related to apoptosis or anoikis and activation of the apoptosis signaling pathway.
We show that a high-sensitivity microarray platform enables analysis of SM tissue samples from individual donors and derived cultures at whole transcriptome level. Microarrays deliver the differential transcriptome. The main findings are that SM cultures show reductive metabolic and muscle-system transcriptome adaptations as observed in muscle atrophy. The metabolic reduction strongly affects key genes of the catabolism of glucose and lipids and moderately and extensively affects genes involved in mitochondrial energy production. In contrast, SM cultures show augmented tissue remodeling transcriptome and induction of genes involved in the apoptosis or anoikis process. Finally, the hypoxia-response pathway appears to participate in this adaptation. Biological pathway analysis by IPA provides insights into processes driven by the culturing of SM such as mitochondrial dysfunction and pathways related to cell interaction with the extracellular matrix and apoptosis. This study contributes to the definition of the phenotype of the SM primary culture, which is a valuable cell model that has key implications for the study of muscle pathogenesis and therapeutic assays.
FR contributed to statistical analysis, validation of gene expression, pathway analysis and manuscript drafting. SM contributed to microarray sample processing and validation of gene expression analysis. MK contributed to study design and manuscript drafting. JC and AN contributed to conception of the study and sample recruitment. EM contributed to sample processing and validation of gene expression analysis. CGM contributed to conception and design of the study and SM cultures. AMGF contributed to conception and design of the study, data analysis and manuscript drafting. All authors have read and approved the final manuscript.
Figure S1. Immunostaining of muscle cultures with the muscle-specific marker desmin. Cultured cells were immunostained with desmin antibody. Then, to highlight nuclei, cells were stained with Hoescht. Representative immunofluorescence micrographs of SM cultures (A) B19, (B) B22, (C) B24, (D) B25 and (E) B26 are shown. Bar represents 50 μm. Most of the Hoescht-stained nuclei are observed in desmin-labeled myotubes.
Table S1. Complete list of differentially expressed genes after skeletal muscle culture. List of the complete set of genes with differential expression in cultured myotubes compared to SM tissue. Replicated genes, which occur more than once in the microarray, were filtered and the first one appearing in the list was arbitrarily chosen. For each gene, the fold change in gene expression was calculated as the ratio between mean values in cultured myotubes versus SM biopsy. The significance of differences was estimated by a Benjamini-Hochberg corrected paired t-test (p < 0.01). The first column lists the ID for Entrez Gene, the second column the gene name, the third column the description of the gene, the fourth column the quality of expression (1) or not (0) in skeletal muscle tissue (M), according to the IPA knowledge-base, and the fifth column the fold change (FC) with a negative symbol for downregulated genes and no symbol for upregulated genes.
Figure S2. Cluster and correlation analysis for microarray data. A) Principal component analysis plot: X = principal component 1, Y = principal component 2 and Z = principal component 3. The percentage of total variance that each principal component captures is 95.9% for component 1, 2.15% for component 2 and 1.90% for component 3. B) Absolute correlation dendrogram. C) Pearson correlation matrix of all samples based on whole gene expression profiles. D) Pearson correlation coefficients between all samples. A value of 1 would mean a perfect correlation. In panels A and C, the samples from the cultured myotube group (in vitro) are represented in red and the samples from the SM tissue biopsies (in vivo) are represented in blue.
Table S2. Most significantly regulated gene ontologies. List of gene ontologies annotated from (A) the whole set or (B) the subset filtered by expression in SM, on the basis of the IPA knowledge-base, of downregulated and upregulated transcripts in cultured myotubes compared to the SM tissue. We used the GO database http://www.geneontology.org with the GeneSpring GX software. GeneSpring GX calculated enrichment scores for GO terms based on the list of regulated genes, and used enrichment scores and Benjamini-Yekutieli (False Discovery Rate) corrected p-values to filter the set of genes. GO terms that are enriched with a p-value cut-off of 0.1 are shown. Less specific nodes in the GO hierarchy that contained the same annotated genes as the stated most-specific nodes are not shown. The first column lists the GO symbol, the second the GO term, the third the corrected p-value and the fourth the count in the selection.
Table S3. Microarray data variability of the 10% most skeletal muscle-filtered differentially expressed transcripts. Log2 normalized intensities for each significantly regulated transcript (p < 0.01 and fold change > 2) in each sample. The standard deviation for each condition, in vitro and in vivo, is shown. A normalized intensity value of 0 means that the raw signal was below the background and an arbitrary value of 1 was assigned to enable the log2 transformation (log2(1) = 0).
We thank Anna Orozco (Bioquímica i Biologia Molecular, Universitat de Barcelona) for technical assistance. This work was supported by grants SAF2006-07228 from the Ministerio de Ciencia y Tecnología (MCyT), SAF2009-07559 from the Ministerio de Ciencia e Innovación and CIBER de Diabetes y Enfermedades Metabólicas Asociadas which is an ISCIII project, Spain. CGM was the recipient of a Ramon y Cajal fellowship from the Ministerio de Educación y Ciencia, Spain. We thank Enrique Blanco (Departament de Genètica, Facultat de Biologia, Universitat de Barcelona) for assistance with the bioinformatic analysis. We thank Manel Bosch and Nieves Hernández from the Unitat de Microscòpia Confocal of the Serveis Cientificotècnics (Facultat de Biologia, Universitat de Barcelona) for assistance with the immunocytochemistry analyses.