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Logo of hhmipaAbout Author manuscriptsSubmit a manuscriptHHMI Howard Hughes Medical Institute; Author Manuscript; Accepted for publication in peer reviewed journal
 
Nature. Author manuscript; available in PMC 2010 June 24.
Published in final edited form as:
PMCID: PMC2835771
HHMIMSID: HHMIMS157065

Dynamics of nucleosome remodelling by individual ACF complexes

Abstract

The ATP-utilizing chromatin assembly and remodelling factor (ACF) functions to generate regularly spaced nucleosomes, which are required for heritable gene silencing. The mechanism by which ACF mobilizes nucleosomes remains poorly understood. Here we report a single-molecule FRET study that monitors the remodelling of individual nucleosomes by ACF in real time, revealing previously unknown remodelling intermediates and dynamics. In the presence of ACF and ATP, the nucleosomes exhibit gradual translocation along DNA interrupted by well-defined kinetic pauses that occurred after approximately 7 or 3 – 4 base pairs of translocation. The binding of ACF, translocation of DNA, and exiting of translocation pauses are all ATP-dependent, revealing three distinct functional roles of ATP during remodelling. At equilibrium, a continuously bound ACF complex can move the nucleosome back-and-forth many times before dissociation, indicating that ACF is a highly processive and bidirectional nucleosome translocase.

The packaging of DNA into chromatin represses essential nucleic acid transactions, such as transcription, replication, repair and recombination. This repression is in part regulated by chromatin remodelling enzymes, which couple the energy of ATP hydrolysis to the assembly and mobilization of nucleosomes. ATP-dependent chromatin remodelling enzymes can be classified into several subfamilies, SWI/SNF, ISWI, CHD/Mi2 and INO80, depending on their composition and function15 . Despite possessing a conserved superfamily 2 ATPase subunit that facilitates DNA translocation6,7, different subfamilies exhibit divergent remodelling activities. For example, the ISWI enzymes have been shown to translocate the histone octamer along DNA and generate a repositioned nucleosome with a canonical structure811, whereas the SWI/SNF enzymes generate a variety of products including repositioned nucleosomes, alternative nucleosome structures containing DNA loops, and nucleosomes with altered histone composition15. The kinetic intermediates and pathways through which the nucleosome structure evolves during remodeling, however, remain largely elusive. Single molecule experiments are ideally suited to probe these dynamics. Recently, optical and magnetic tweezers have been used to study individual SWI/SNF remodelers, providing direct measurements of DNA translocation and loop formation by these enzymes1214. In this work, we established a single-molecule Förster resonance energy transfer (FRET)1517 assay to characterize the structural dynamics and kinetic intermediates of nucleosomes during remodelling. Human ACF1822, a representative member of the ISWI family remodelers, was investigated using this approach.

Probing nucleosome translocation by FRET

For FRET characterizations, we labelled histone octamers with a donor dye (Cy3) on histone H2A23 and reconstituted mononucleosomes with the Cy3-labeled octamer and a double-stranded DNA that contained an acceptor dye (Cy5) and a biotin at opposite ends. Unless otherwise indicated, we used the 601 nucleosome positioning sequence24 to place the octamer 3 base pairs (bp) away from the Cy5-labelled exit end of the DNA, leaving 78 bp of linker DNA on the entry side (Fig. 1a, Supplementary Fig. 1a, n = 3 bp). The nucleosomes were then anchored to a microscope slide via a biotin-streptavidin linkage and imaged by a total-internal-reflection-fluorescence (TIRF) microscope25. The presence of two H2A subunits in each octamer led to a heterogeneous population of nucleosomes with three different labelling configurations: 1) donor on the H2A subunit proximal to the acceptor, 2) donor on the H2A subunit distal to the acceptor, 3) donor on both H2A subunits. Single-molecule detection allowed these configurations to be discriminated. Three distinct peaks centred at FRET = 0. 88, 0.75 and 0.58 were observed in the FRET distribution (Fig. 1b). The assignment of these peaks to the three labelling configurations was further confirmed by individual FRET time traces, which showed one- or two-step photobleaching for nucleosomes bearing one or two donor dyes, respectively (Supplementary Fig. 2). In the following, we focus our analyses on nucleosomes containing a single donor on the proximal H2A (FRET = 0.88) to maximize the dynamic range in our experiments.

Figure 1
Monitoring ACF-catalyzed nucleosome remodelling by single-molecule FRET

Recombinant ACF, comprised of a catalytic ATPase subunit, SNF2h, and an accessory subunit, Acf11922, was added to the surface-anchored nucleosomes to induce remodelling. FRET decreased substantially upon addition of ACF and ATP (Fig. 1b), whereas incubation with ACF alone resulted in no significant change in FRET (data not shown). The observed decrease in FRET is consistent with the ability of ACF to centre mononucleosomes on DNA10,11,23,26,27 (Fig. 1a). The average remodelling rate measured from nucleosomes anchored to the surface was quantitatively similar to that determined from measurements of nucleosomes in solution, indicating that surface-anchoring of nucleosomes did not inhibit the activity of ACF (Supplementary Fig. 3).

In order to correlate the observed FRET value to the octamer position quantitatively, we measured FRET for a series of nucleosome constructs with different linker DNA lengths (n) on the exit side (Fig. 1c). The FRET value decreased monotonically with increasing exit linker length in a manner similar to the distance-dependence of FRET observed between donor and acceptor dyes attached to a DNA duplex (Supplementary Fig. 4). To further test whether the ACF-induced FRET change was indeed due to translocation of the histone octamer on DNA, we designed nucleosomes with stall sites defined by single-stranded (ss) DNA gaps. It has been shown that the ATPase domain of ISWI remodellers contacts a DNA region two helical turns (~20 bp) from the dyad axis of the nucleosome, and that ssDNA gaps located in this region inhibit nucleosome translocation2830. We thus prepared a series of nucleosomes with the same linker DNA lengths (78 bp on the entry side and 3 bp on the exit side), each possessing a two-nucleotide ssDNA gap at a specified distance (m bp) away from the dyad axis (Fig. 1d). While the initial FRET values of these constructs were similar to that observed for the construct without the ssDNA gap, the final FRET values after remodelling showed a strong dependence on the position of the ssDNA gap (Fig. 1d), with little FRET change for the construct with m = 20 bp and a FRET versus m slope identical to that observed for the exit linker length dependence shown in Fig 1c. These results demonstrate that the observed ACF-induced FRET changes can be quantitatively interpreted in terms of nucleosome translocation along DNA, though we cannot formally exclude the possibility that other alterations in nucleosome structure could also make a minor contribution. An interesting consideration is the spontaneous site exposure due to fraying DNA ends previously reported to occur in the 0.01 – 0.05 s time scale31, which should not cause significant fluctuations in FRET observed here with 0.1 – 2 s time resolution.

Multiple ATP-dependent remodelling steps

Next we characterized the remodelling kinetics by adding ACF and ATP to the nucleosomes in situ during data acquisition. After the addition of ACF and ATP, individual nucleosomes exhibited a “waiting” period prior to any detectable change in FRET, followed by a “translocation” period, during which FRET decreased to the background level (Fig. 2a). The duration of the waiting period (twait) depended on both ACF and ATP concentrations (Fig. 2b). The distributions of twait obtained at various ACF and ATP conditions suggest that the waiting phase included at least two steps, one depending on the ACF concentration and the other on ATP (Supplementary Fig. 5). To determine the order of these two steps, we performed a three-colour experiment with dye-labelled ACF, in which signal from the Alexa 488 dye on ACF directly reported the binding of the enzyme, while the FRET pair on the nucleosome reported the nucleosome position on the DNA. Notably, the binding of ACF preceded the onset of FRET decrease (Fig. 2c). Both the time before ACF binding (tbind) and the time lag (tlag) from ACF binding to the onset of FRET decrease depended on the ATP concentration (Fig. 2c), indicating that the waiting phase consisted of an ATP-dependent ACF binding step followed by an additional ATP-dependent step after the enzyme bound.

Figure 2
Real-time dynamics of ACF-catalyzed nucleosome translocation

In contrast to the waiting phase, the duration of the translocation phase (ttranslocate) was only dependent on ATP, but not on ACF, concentration (Fig. 2d and Supplementary Fig. 6), suggesting that binding of additional ACF molecules was not required during this phase. Consistent with this notion, when we prebound nucleosomes with ACF and then removed unbound ACF with a buffer containing ATP to initiate remodeling, the majority (86%) of the remodelled nucleosomes showed a complete decrease in FRET to below 0.1, indicating that the translocation phase did not require binding of additional ACF from the solution.

Translocation pauses during remodelling

Notably, translocation of the nucleosome did not proceed at a constant rate. Instead, the translocation phase exhibited periods of gradual decrease in FRET interrupted by translocation pauses (Fig. 3). For nucleosomes with the initial exit linker length n = 3 bp, the first pause occurred at a FRET value of 0.53 ± 0.03 (Fig. 3a, b), corresponding to an increase of linker length to 9.9 ± 0.6 bp and thus nucleosome translocation by 6.9 ± 0.6 bp. The pause position appeared to be independent of the initial linker length: for nucleosome constructs with four different linker DNA lengths (n = −3, 0, 3 and 6 bp), the first pause all occurred after approximately 7 bp of DNA translocation (Supplementary Fig. 7a).

Figure 3
ACF-catalyzed nucleosome translocation is interrupted by well defined kinetic pauses

In addition, we tested the dependence of the pause position on DNA sequence using a weaker positioning sequence “A-100” (Supplementary Fig. 1b), which has ~100 fold lower affinity than the 601 sequence32. The first pause of these nucleosomes again occurred after approximately 7 bp of translocation (Supplementary Fig. 7b). While we cannot formally rule out the possibility that the positioning sequences contributed to the position of this initial pause, the observation that nucleosomes with two substantially different DNA sequences exhibit the same initial pause position suggests a potentially general feature of remodelling by ACF.

In addition to the first pause, subsequent translocation pauses were observed at lower FRET values (Fig. 3a, c). For nucleosomes with initial exit linker length n = 3 bp, a second and third pause preferentially occurred at FRET = 0.34 ± 0.03 and 0.17 ± 0.03, corresponding to 3.8 ± 0.6 bp and 3.3 ± 0.6 bp of translocation prior to pausing, respectively (Fig. 3a, b). Similar pauses were also observed for the n = −3 bp nucleosomes, except that the shorter exit linker length after the third pause allowed detection of a fourth pause, which occurred after 3.6 ± 0.8 bp of translocation from the third pause (Figs. 3c, d). Taken together, these results indicate that the nucleosomes were translocated by a shorter distance (3 – 4 bp) between the subsequent pauses. Both the dwell time of the pauses and the duration of the translocation phases in between pauses depended on the concentration of ATP, indicating that ATP binding was required in both phases (Fig. 3e and Supplementary Fig. 8). The dwell times of the subsequent pauses were similar to each other but substantially shorter than that of the first pause.

We note that the sum of a 7 bp and a 3 – 4 bp step and the sum of three 3 – 4 bp steps are both close to the 10 bp periodicity of DNA-histone contacts within the nucleosome33. Interestingly, the remodelling intermediates at a fraction of the periodicity (7 bp and 3 – 4 bp) were not stable in the absence of the remodelling enzyme: upon removal of ACF, these intermediates collapsed to nucleosomal states in which the histone octamer was repositioned by a multiple of ~10 bp from the pre-remodelling position (Supplementary Fig. 9). These collapsed states, consistent with the previously observed accumulation of remodelling products at ~10 bp intervals of nucleosome translocation28,29, are likely imposed by structural constraints of the nucleosome.

Processive and bidirectional translocation

The above experiments with end-positioned nucleosomes provide quantitative analyses of remodelling kinetics and intermediates. The limited dynamic range of FRET, however, made it difficult to characterize the equilibrium state(s) after remodelling using these substrates. Considering that ACF tends to centre the nucleosome on the DNA, we reasoned that a centre-positioned nucleosome with an initial FRET value within the dynamic range of FRET would facilitate the analysis of equilibrium remodelling dynamics. To this end we constructed a centre-positioned mononucleosome with the 601 sequence flanked by 78 bp of DNA on each side and an internal acceptor label (Fig. 4a, Supplementary Fig. 1a)). The initial FRET distribution showed a narrow peak at FRET = 0.3 (Supplementary Fig. 10). After equilibration with ACF and ATP, the FRET distribution broadened substantially (Supplementary Fig. 10) and the time traces of individual nucleosomes exhibited large-amplitude oscillations in FRET (Fig. 4b), indicating that the histone octamer was translocated back-and-forth along the DNA by the remodelling enzyme. Bidirectional remodelling was observed to be the predominant behaviour (> 70% of remodelled nucleosomes), even at sub-saturating conditions in which a low concentration (1 nM) of ACF was added to induce remodelling of only a small fraction (<10%) of the nucleosomes. Autocorrelation analysis of these FRET time traces revealed a characteristic oscillation time that was dependent on the ATP concentration but independent of the ACF concentration (Fig. 4b), suggesting that the observed bidirectional translocation was accomplished by continuously bound ACF without requiring dissociation and rebinding of ACF from the solution. To further test this notion, we performed three-colour experiments with Alexa 488-labelled ACF and FRET-labelled nucleosomes, in which signal from the Alexa 488 dye directly reported the binding of ACF. Repeated back-and-forth movement of the nucleosomes was observed within individual ACF binding events (Supplementary Fig. 11), further confirming that the bidirectional nucleosome translocation was accomplished by a continuously bound ACF complex.

Figure 4
ACF catalyzes processive and bidirectional nucleosome translocation

To further quantify the processivity of ACF, we performed buffer exchange experiments in which ACF and ATP were added and unbound ACF (but not ATP) was subsequently removed in situ as the position of individual nucleosomes was monitored. Remarkably, the ACF-induced bidirectional movement persisted for a long period of time after unbound ACF was removed from the solution (Fig. 4c). The nucleosomes were translocated with an average speed of approximately 2 bp/s. The lower-bound estimate of the cumulative distance travelled by the nucleosome after removal of unbound ACF exhibits a broad distribution with a mean of 200 bp (Fig. 4c). Taken together, these results indicate that ACF is a highly processive and bidirectional nucleosome translocase. The observed processivity is consistent with the strong commitment of ISWI enzymes to nucleosomal templates once chromatin assembly and remodelling are initiated34,35.

It is striking that an ACF complex remaining bound to the nucleosome could cause such a highly processive, back-and-forth nucleosome movement. Such a demanding task could be accomplished if ACF preferentially binds the nucleosome as a dimer, in which two ACF monomers, particularly their corresponding ATPase domains, are bound on opposite sides of the nucleosome and oriented for translocation in opposing directions. Coordinated action of the two monomers would then allow processive back-and-forth translocation of the nucleosome. This hypothesis is supported by our three-colour experiments with Alexa 488-labelled ACF and FRET-labelled nucleosomes. To determine the number of ACF bound to the nucleosome, we performed statistical analyses of the Alexa 488 intensity and the number of Alexa 488 photobleaching steps associated with each ACF binding event. These analyses suggest that the binding events leading to bidirectional nucleosome remodelling preferentially contained two ACF monomers, whereas the binding events leading to unidirectional remodelling preferentially contained a single ACF monomer (Supplementary Fig. 12). Further supporting this model, electron microscopy and biochemical data showed cooperative binding of two SNF2h proteins to a single nucleosome with each SNF2h occupying one side of the nucleosome in an activated ATP state (Racki et al manuscript). The diffusion coefficient of ACF bound to DNA is also consistent with a complex of two Acf1 and two SNF2h subunits36. Interestingly, the SWI/SNF subfamily enzymes can also reversibly create and retract DNA loops12,13, but it is unclear whether the bidirectional nucleosome translocation by ACF and the reversible DNA loop formation by SWI/SNF share a common mechanism.

Discussion

We have developed a single-molecule assay to monitor the remodelling of individual nucleosomes by chromatin remodelling enzymes in real time. This assay allowed quantitative characterization of the structural dynamics and kinetic intermediates of nucleosomes during remodelling. Using this approach, we showed that the human ACF enzyme induced gradual translocation of nucleosomes along DNA interrupted by well-defined kinetic pauses. ATP plays multiple functional roles in the remodelling process. The three distinct steps during remodelling, namely binding of ACF, translocation of the nucleosome, and translocation pauses, were all ATP dependent, revealing a versatile usage of ATP by an enzyme with only one type of ATP binding site.

Quantification of the FRET traces of end-positioned nucleosomes showed that the first kinetic pause occurred after approximately 7 bp of nucleosome translocation whereas subsequent pauses were separated by only 3 – 4 bp. Although it is currently unclear whether these remodelling intermediates occur only at the beginning of remodelling or continue into the processive remodelling phase, similar translocation pauses were also observed during the continuous remodelling process of centre-positioned nucleosomes (Fig. 4b) and thus may represent a fundamental property of ACF-induced remodelling. One possible origin of these intermediates is an ATP-dependent conformational change of the remodelling enzyme that prepares the nucleosome for the next round of DNA translocation (for example, by forming a DNA loop for subsequent propagation around the nucleosome)30,37,38. The unique properties of the first pause, as compared to the subsequent pauses, may imply a complex initiation phase of remodelling.

On centre-positioned nucleosomes, ACF was observed to exhibit remarkable processivity and bidirectionality: an ACF complex continuously bound to a nucleosome could translocate the histone octamer back-and-forth by a total distance of more than 200 bp and switch directions more than 20 times on average before dissociation. Statistical analyses suggest that the bidirectional remodelling is most probably caused by ACF dimers. The processive and bidirectional translocation of nucleosomes potentially allows ACF to rapidly sample the DNA on both sides of the nucleosome to generate regular inter-nucleosomal spacing.

METHODS SUMMARY

Detailed description of sample preparation and single-molecule FRET measurements are given in Online Methods. Briefly, various mononucleosome constructs, with different DNA sequences, DNA linker lengths, and ssDNA gap locations, were reconstituted using histone octamers that were labelled with a green FRET donor dye (Cy3) and double stranded DNA that was labeled with a red FRET acceptor dye (Cy5) and a biotin. The nucleosomes were then anchored to a microscope slide via a biotin-streptavidin linkage. Unlabelled ACF or ACF labelled with a blue dye (Alexa 488) were added to the surface-anchored nucleosomes together with ATP to induce remodelling. The fluorescence signals from Alexa 488, Cy3, and Cy5 were detected by a TIRF microscope, separated by dichroic mirrors, and imaged onto separate areas of an amplified-CCD camera after passing through various fluorescence emission filters. Custom-written software was used to identify single nucleosomes on the slide and to monitor the Alexa 488, Cy3 and Cy5 fluorescence at these positions for extended periods of time. The FRET value was defined as IA / (ID + IA), where ID and IA represent the fluorescence signals detected in the Cy3 and Cy5 channels, respectively.

Supplementary Material

01

Acknowledgements

We thank J. Widom (Northwestern University) for providing the plasmid containing the 601 positioning sequence and R.E. Kingston (Harvard Medical School) for the plasmids containing SNF2h and Acf1 genes. We also thank Lisa Racki and Elio Abbondanzieri for helpful discussions, and William Huang and Bryan Harada for help with some experiments. This work is supported in part by Howard Hughes Medical Institute (to X.Z.) and the National Institutes of Health (GM073767) and the Beckman Foundation (to G.J.N). X.Z. is a Howard Hughes Medical Institute investigator. M.D.S. was a NIH Ruth L. Kirschstein NSRA Fellow, G.J.N is a Leukemia and Lymphoma Society Scholar.

Footnotes

Supplementary Information is linked to the online version of the paper at www.nature.com/nature.

Author information T.R.B. performed the experiments and analysis with help from M.D.S. J.G.Y. made the enzymes and histone proteins. T.R.B., G.J.N, and X.Z. designed the experiments. X.Z. oversaw the project.

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