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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
J Am Coll Cardiol. Author manuscript; available in PMC 2011 February 9.
Published in final edited form as:
PMCID: PMC2833093

Evaluation of Cuff-Induced Ischemia in the Lower Extremity by Magnetic Resonance Oximetry

M. C. Langham, PhD,* T. Floyd, MD,** E. R. Mohler, III, MD,** J. F. Magland, PhD,* and F. W. Wehrli, PhD*



The aim of this study was to evaluate vascular function in the lower extremities by making direct time-course measurement of oxygen saturation in the femoral/popliteal arteries and veins during cuff-induced reactive hyperemia with MRI-based oximetry.


MRI-based oximetry is a new calibration-free technique taking advantage of the paramagnetic nature of blood that depends on the volume fraction of deoxyhemoglobin in red blood cells.


We compared postocclusive blood oxygenation time-course of femoral/popliteal vessels in (1) young healthy subjects (YH, N =10; mean ABI 1.0 ± 0.1, mean age 30 ± 7 y), (2) peripheral arterial disease patients (PAD, N = 12; mean ABI 0.6 ± 0.1, mean age 71 ± 9 y) and 3) age-matched healthy controls (AHC, N = 8; mean ABI 1.1 ± 0.1, mean age 68 ± 9 y). Blood oxygenation was quantified at 3T field strength with a field mapping pulse sequence yielding the magnetic susceptibility difference between blood in the vessels and surrounding muscle tissue from which the intravascular blood oxygen saturation is computed as %HbO2.


Significantly longer washout time (42 ± 16 vs. 14 ± 4 s; p < 0.0001) and lower upslope (0.60 ± 0.20 vs. 1.32 ± 0.41 %HbO2/s; p = 0.0008) were observed for PADs compared to healthy subjects (YH and AHC combined). Further, greater overshoot was observed in YH than in AHC (21 ± 8 vs. 10 ± 5 %HbO2; p = 0.0116).


Postocclusive transient changes in venous blood oxygenation may provide a new measure of vascular competence, which was found to be reduced in subjects with abnormal ABI, manifesting in prolonged recovery during the early phase of hyperemia.

Keywords: peripheral arterial disease, magnetic resonance oximetry, blood oxygen saturation, reactive hyperemia, phase image

In United States the number of adults affected by peripheral artery disease (PAD) is as large as 8 million (1), a number expected to rise as the elderly population grows. PAD reduces the quality of life of individuals, but more importantly, the relative risk of cardiovascular events significantly increases. For the above reasons, a noninvasive method of detecting of early manifestations of atherosclerosis in the lower extremity is desirable. Specifically, a quantitative prognostic tool to identify individuals more susceptible to the major cardiovascular risk factors (CRF) is desirable since the Framingham score fails to account for 50% of the mortality and morbidity (2). The initial test used to diagnose PAD is the ankle-brachial index (ABI) measurement, which is calculated as the ratio between systolic pressures of ankle and brachial artery. The ABI is simple, inexpensive, painless, reproducible, and can be easily performed in a physician’s office. However, the ABI measurement has some limitations as there is a high specificity but low sensitivity in population-based cohorts (no evidence of pre-existing PAD) (3). In addition, the ABI may be falsely elevated in patients with medial artery calcification. Additional diagnostic tools are needed that provide early assessment of lower extremity PAD prior to development of flow limiting lesions.

The assessment of vascular function at rest is difficult because metabolic rate and capillary blood flow in the extremities is not significantly different between PAD and healthy subjects. Even with multiple levels of hemodynamically significant stenoses, nutritive requirements can be maintained via establishment of collateral circulation. However, the high peripheral resistance seen with occlusive PAD cannot accommodate greater transient flow rates induced by a physiological challenge, such as a cuff-compression or exercise paradigm.

In order to reoxygenate hypoxic tissue after a period of ischemia, the blood flow is markedly increased through reduction of microvascular resistance. The resulting increase in shear stress to the endothelium after cuff release leads to vasodilation of the conduit artery mainly via NO release (4). Thus, reactive hyperemia offers an opportunity to assess micro- and macrovascular function. Non-invasive imaging modalities that have been used to quantify reactive hyperemia include near-infrared spectroscopy (NIRS) (58), single photon emission computed tomography (SPECT)(9), MRI (10,11), and ultrasound(12). NIRS and ultrasound are most commonly used due to their portability. NIRS is relatively inexpensive, has excellent temporal resolution and is less hampered by subject motion. However, the spatial resolution of NIRS is low (on the order of centimeters) and can only provide regional information. The separation of the source-detector probes determines the depth of the layer being probed and the true path of the infrared light is not known (5). Further, tissue response to the light depends on skin color, body fat and muscle layers, leading to large inter-subject variations and inability to focus on specific vessels or distinguish between arterial and venous saturation. Thus, NIRS allows for the examination of microvascular function in relatively superficial tissues only. Ultrasound measurements of flow-mediated dilatation (FMD) in the brachial artery have generated substantial interest in clinical research since its introduction (13). Several studies have since demonstrated the prognostic value of brachial artery FMD (14,15), however, the methodology suffers from substantial inter- and intra-observer variability (e.g. transducer placement) (4,16) and limited spatial resolution. Both effects are magnified since the relative change of FMD averages less than 5% (17). Similar to ultrasound quantitative MRI flow velocimetry can be performed with high spatial and temporal resolution as demonstrated in a recent study in which post-occlusion hyperemia in the femoral artery was evaluated in PAD patients (11).

We evaluated vascular function with a time-course measurement of oxygen saturation in the femoral/popliteal vessels with the aid of a new quantitative magnetic resonance technique that makes use of deoxyhemoglobin’s paramagnetism (18). MRI oximetry (MRIO) quantifies blood oxygen saturation by spatially mapping the magnetic susceptibility of blood, which scales linearly with (1-HbO2). Blood oxygen saturation is obtained by modeling the vessel as a long paramagnetic cylinder immersed in an external field, and relies on the quantification of the induced field of the blood in the vessel relative to surrounding muscle tissue, achieved by measuring the phase of the MR signal. Figure 1 illustrates the basic principles of MRIO for quantification of blood oxygen saturation. The feasibility of this approach as well as the method’s accuracy and precision have recently been demonstrated, in phantoms and in vivo in the authors’ laboratory (1921). The present study extends the technique to quantify blood oxygenation during cuff-induced reactive hyperemia in the femoral/popliteal artery and vein.

Figure 1
Principle of MR oximetry

We hypothesize that washout time, upslope and overshoot derived from blood oxygenation time-course will provide quantitative assessment of the vascular competence during the hyperemic period following cuff-induced ischemia. The washout time refers to the time required to observe oxygen-depleted blood in the tissue to pass through the imaging slice once flow is restored. The upslope is the subsequent reoxygenation rate and the overshoot represents the transient increase in venous saturation relative to the baseline value. To test the hypothesis, the above parameters were derived in a pilot study to determine whether PAD patients, diagnosed on the basis of abnormal ankle-brachial index (ABI), differ from age-matched controls (AHC), and whether the latter differ from young healthy (YH) subjects. The latter comparison is motivated by the fact that aortic stiffness is known to increase with age (22) and there is a growing evidence relating increased pressure pulsatility to reduced peripheral reactive hyperemia (23).


Patient Selection

Three groups of subjects were compared: 1) PAD (N = 12, mean ABI 0.6 ± 0.1, mean age 71 ± 9, 3 females) and 2) AHC (N = 8, mean ABI 1.1 ± 0.1, mean age 68± 9, 2 females), 3)YH (N = 10, mean ABI 1.0 ± 0.1, mean age 30 ± 7, 4 females). Additional patient data are summarized in Table 1. Young healthy subjects (ages 20 – 40) and age-matched controls were recruited based on their ABI (> 0.90 in either leg) and medical history (normotensive and without prior cardiovascular events). PAD subjects, males and females with a history of PAD, were defined by having ABI < 0.80. Patients with a history of myocardial infarction or stroke within three months prior to study enrollment, or vascular surgery on leg selected for scanning were excluded. The ABI of each leg was calculated by dividing the pressure of the dorsalis pedis artery by the higher of the two brachial artery blood pressures. Blood pressure was recorded using Doppler ultrasound when the pulse became audible after deflating the cuff at 20 mmHg above the last audible pulse. Written informed consent was obtained prior to all examinations following an institutional review board-approved protocol.

Table 1
Patient characteristics.

Reactive hyperemia in response to cuff-induced ischemia

In preparation for the examinations, subjects were asked to fast for 12 hours (except for water), during which period they were also asked to abstain from the following: 1) smoking, 2) taking of vasoactive medications (including over-the-counter medications such as high-dose niacin, decongestants, vitamins C and E), and 3) vigorous physical activity. Ischemia was induced in the lower limb by blood pressure cuff (Aspen Labs A.T.S 1500 Tourniquet System) applied on the upper-most part of the thigh to minimize perturbation of the imaging region during cuff inflation or deflation, and inflated to 75 mmHg above systolic pressure, but not to exceeding 250 mmHg. Data were collected with two different occlusion paradigms of either 3 minutes or 5 minutes, which were preceded by 2 minutes of baseline scanning, followed by a 6-minute recovery period (Figure 2). The two different paradigms were performed sequentially, with the 5-minute cuff occlusion following 9 minutes after deflation of the cuff at the end of the 3-minute occlusion period.

Figure 2
Cuff paradigm

MRI Protocol

All MR scans were performed on a 3T Siemens Trio scanner using an eight-channel knee array coil (Invivo Inc., Pewaukee, WI). Images were acquired with a spoiled multi-echo gradient-recalled echo (GRE) pulse sequence (Figure 3) that was programmed with SequenceTree v. 3.1(24). The pulse sequence included fat suppression and flow compensation along the slice direction. Axial images of the lower thigh (approximately 10 cm distal to the distal boundary of the cuff) were acquired with the following parameters: voxel size and resolution = 1 × 1 × 5 mm3, FOV = 128×128 mm2, BW = 488 Hz/pixel, four echoes (TE1 = 4.5ms), echo spacing of 2.32ms, temporal resolution of 5, 10 or 20s achieved with TR = 39, 78 or 156 ms and radiofrequency pulse flip angles = 13, 19 or 26°, respectively. Baseline scanning was performed with TR of 156 ms (20 s temporal resolution), subsequently switched to 78 ms (10 s resolution) during cuff occlusion (Figure 2). TR was lowered further to 39 ms about 10s before and the first 120 seconds after cuff deflation to capture the rapid transient changes during hyperemia, providing a temporal resolution of 5s. During the remaining recovery period, data was collected first at 10s, and subsequently at 20s temporal resolution. In this manner data size could be minimized during this relatively quiescent period without risking loss of essential information. Prior to execution of the cuff-inflation paradigm, 20 contiguous scout images were collected at the same spatial resolution, but under conditions ensuring maximum vessel visibility (i.e. at high flip angle to maximize flow-related enhancement) for location of the optimum slice as well as to estimate vessel tilt angle (21).

Figure 3
Field-mapping pulse sequence

Data Analysis

The raw k-space data were saved and processed offline. From the four complex images (each being associated with an echo in the pulse sequence of Figure 3), two phase difference images were constructed(25) and the phase from each pixel averaged to yield:


where Zi represents complex value of a particular pixel from the image of the ith echo and the asterisk denotes complex conjugate. The most significant source of error results from low-frequency modulations of static magnetic field produced by the interface between air and tissue or between adjacent tissue types. The slowly-varying modulation was fit to a 2nd-order polynomial(20) and subtracted from Δ[var phi] map before measuring the phase difference between the intravascular phase and average phase of nearby tissue region (Figure 1). The absolute %HbO2 in both vein and artery then was computed using

%HbO2=[12[mid ]Δ[var phi][mid ]/ΔTEγΔχdoHct·Bo(cos2θ1/3)]×100

where Δ[var phi] is the average intravascular phase relative to surrounding tissue, ΔTE is the echo spacing between the equal-polarity echoes, γ is the gyromagnetic ratio of water protons, Δχ do = 4π (0.27 ± 0.02) ppm (26) is the susceptibility difference between fully deoxygenated and fully oxygenated erythrocytes, and θ is the vessel tilt angle relative to the main field Bo. Since the femoral and popliteal vessels are not exactly parallel to Bo, the local vessel tilt was measured from the scout images from the coordinates of the vessel’s centroid in slices separated by approximately 3 cm. Hematocrit (Hct) was determined from a sample of blood drawn from each subject, in the institution’s Clinical and Translational Research Center.

To quantify vascular competence during reactive hyperemia washout time, upslope and overshoot, defined in Figure 4, were derived from the venous oxygenation time-course. The washout time refers to the time required for the deoxygenated blood from the hypoxic tissue to reach the scan location, the upslope is the reoxygenation rate and the overshoot is the peak venous saturation during hyperemia relative to the average baseline value. Results of upslope, washout and overshoot were analyzed (SAS Institute Inc. JMP 7.0) to determine statistical significance between groups using a Kruskal-Wallis test (non-parametric one-way ANOVA) (p < 0.05). For simplicity, we performed pair-wise post hoc Wilcoxon tests to evaluate group differences but using a more stringent significance criterion of p < 0.025.

Figure 4
Parameters of reactive hyperemia

Accuracy and precision of MR oximetry has previously been investigated by some of the present authors (21). In phantoms the effect of vessel non-circularity was found to be negligible for tilt angles <30°, in agreement with the theory and the coefficient of variation in repeat measurements was <2%, achieved with tilt correction alone. In vivo, repeated measurements of %HbO2 in the femoral vessels performed at multiple levels (where vessel segments differed in geometry such as eccentricity and tilt angle) yielded an average coefficient of variation from three subjects of less than 5%.


Representative magnitude and phase difference images (after correction for regional field inhomogeneity) are shown in Figure 5. Greater phase (corresponding to lower oxygen saturation) in the femoral vein is seen compared to the artery. The phase contrast between artery and surrounding reference tissue is virtually imperceptible, as expected, since muscle tissue and fully oxygenated blood have approximately equal magnetic susceptibility. The average baseline %HbO2 values of venous and arterial oxygenation for all non-PAD subjects were 64 ± 8 and 95 ± 3 %, respectively, and did not differ significantly from those of PADs, 67 ± 9 % and 94 ± 4 %. These observations are not surprising since it is well known that the oxygenation levels at rest do not differ between healthy subjects and PAD patients. This is also in agreement with near-infrared muscle oxygenation studies (8,27).

Figure 5
Sample images

Figure 6 shows the time course of arterial and venous oxygen saturation in three representative subjects (one from each group), before, during and after cuff-induced ischemia. The average values of the washout time, upslope and overshoot for each group are summarized in Table 2. The overshoot after 5-minute occlusion was the only quantity that was significantly different between YH and AHC (21 ± 8 vs. 10 ± 5 %HbO2; p = 0.0116). Based on this result, YH and AHC were combined as a “healthy” group (Table 2 Column 4) who were compared to PAD subjects. The latter were characterized by significantly longer washout time and smaller upslope, after both 3- and 5-minute cuff occlusions, when compared to subjects with normal ABI. Following 5 minutes of cuff-compression, the average washout time for PAD subjects was 42 ± 16 s compared to 14 ± 4 s (p < 0.0001) for healthy subjects. Washout time in PAD subjects varied widely (SD ± 16 s), reflecting the complexity of PAD, in contrast to healthy subjects where this parameter was narrowly distributed (SD ± 4 s). Finally, the average upslope in the normal group was more than twice that of those with PAD (1.32 ± 0.41 vs. 0.60 ± 0.20 %HbO2/s; p = 0.0008).

Figure 6
Representative time-course of the blood oxygenation – For each group a sample 3- and 5-min (right panel) cuff occlusion time-course is shown.
Table 2
Summary of study results.

Arterial blood oxygenation was found to remain essentially unchanged during the entire time course since no oxygen is extracted from the artery at the location of measurement (oxygen extraction takes place in the capillary bed). On the other hand, disruption of arterial supply during cuff occlusion is expected to result in gradual depletion of the tissue oxygen stores. Therefore, upon cuff deflation there is rapid drainage of the oxygen-depleted capillary blood, resulting in a large transient reduction in venous saturation (%SvO2) at the observation point, a phenomenon observed in all subjects. This short, post-occlusion dip is followed by above-baseline saturation from transient increase in blood flow and subsequent gradual decrease toward baseline values after SvO2 has reached a broad maximum. The large difference in minimum venous saturation between YH and AHC shown in Figure 6 is not a consistent finding. The average relative difference in venous saturation with respect to the baseline value (ΔSvO2)max did not differ (18 ± 8 vs 19 ± 9 %HbO2) between the two groups.


Blood oxygen saturation is an important physiological parameter that can provide valuable information on a variety of clinical conditions including congenital heart defects associated with arterial-venous mixing, hypoxemic conditions, and ischemic diseases affecting various organs such as the heart, brain, kidneys and gut. In this study, we used of oxygen saturation as an endogenous tracer to evaluate peripheral vascular function. A cuff-induced ischemic paradigm was used to assess peripheral arterial disease, because normal blood oxygenation as well as nutritive need can be maintained at rest even in the presence of multiple levels of stenoses through collateral circulation (28). We examined the hypothesis that the rate at which blood in hypoxic tissue is replaced reflects vascular competency. Support for this hypothesis has been provided with the distinguishing behavior of the temporal changes in SvO2 between PAD subjects and their healthy controls, using a quantitative magnetic resonance technique dubbed “MR oximetry.” From the time-course, three parameters characterizing different temporal phases of reactivity were derived: washout time, upslope and overshoot.

Most prior studies examining the effect of occlusion-induced ischemia and its hyperemic response utilized measurement of blood flow and FMD in the conduit vessels with ultrasonography, or oxygenation and perfusion in tissue using NIRS. In the present study, we used a novel method to “label” the blood in the capillary bed via cuff occlusion and followed the fate of the oxygen-depleted blood upon restoration of flow and its subsequent replacement by monitoring the temporal changes in oxygen saturation in the femoral vein. The results reveal that at least two of the derived quantities -- washout time and upslope -- differ significantly between healthy subjects (YH and AHC combined) and those with PAD (Table 2), but do not appear to depend on the duration of cuff occlusion. Neither washout time nor upslope differed significantly between the two healthy subject groups. However, the difference in the post-ischemic overshoot of SvO2 was significant between YH and AHC (21 ± 8 vs. 10 ± 5 %HbO2; p = 0.0116) after 5-minute occlusion but not after a 3 minutes of occlusion (16 ± 9 vs. 9 ± 8 %HbO2; p < 0.16). Nishiyama et al (29) found that the peak post-ischemic blood flow rate in the popliteal artery occurs less than 10s after a 5 minutes of occlusion regardless of age (average age of 26 vs. 72) in healthy subjects but the magnitude was significantly greater in young compared to old subjects and the noticeable difference in the flow rate was maintained for approximately a minute. In a study by Abramson et al (30), the flow rate in the forearm of healthy young subjects was evaluated in response to 2.5-and 5-minute cuff occlusion. The authors found the longer period of tissue hypoxia in the forearm to lead to increased duration of hyperemia as described above and the magnitude of the peak flow, which occurred within 10 – 15s, increased only marginally after a 5-minute occlusion compared to 3 minute. A direct comparison cannot be made between our data and the results of Nishiyama et al(29) and Abramson et al (30) because we are quantifying venous blood oxygenation instead of arterial blood flow rate. However, it is intuitive that the washout time and upslope – parameters that characterize the early phase of hyperemia - are most likely related to the magnitude and time required to achieve peak post-ischemic flow rate.

A common finding among healthy subjects studied is that the early phase of hyperemia is rather brief, i.e. the blood flow rate reaches its peak and then falls down to half the value within approximately 20s (29). This is essentially the time we find in our study for oxygen-depleted blood to reach the imaging region. This explains comparable washout time and upslope among the healthy subjects. Nevertheless, it is conceivable that washout time and upslope are both shorter and greater, respectively, in YH but that the difference is too small to be detected with 5s temporal resolution. Similarly, the observation that a longer occlusion period did not cause increased (ΔSvO2)max among healthy subjects may also reflect the limited temporal resolution.

Finally, the overshoot depends on the blood flow rate because at higher flow rate the tissue has less time to extract oxygen. Nishiyama et al (29) found the blood flow rate during the recovery period to be much greater in young healthy subjects and, according to the work by Abramson et al (30), is maintained longer in proportion to the cuff-occlusion period. Similarly, we found a greater overshoot in the younger compared to the older healthy age group, an effect that was significant for 5 minutes (p = 0.0116) but not for 3 minutes of occlusion (p < 0.16). The age-related reduction overshoot may reflect decline in microvascular function associated with age(31).

The upslope, washout and overshoot in PAD subjects did not appear to depend on the duration of the ischemic period. The effect of PAD on vascular reactivity is complex and not fully understood currently. The combination of microvessel stiffness, collateral circulation, change in endothelial function (NO release (32)), and possibly reduced capillary density (which may occur from chronic ischemia among more serious cases of PAD), may result in “critical damping” of reactive hyperemia, so that longer cuff-compression does not lead to an observable difference in vascular reactivity. The severity of PAD can be classified in terms of three types of vascular reactivity(28) responses following exercise-induced ischemia. In the type-I response the peak blood flow is attained within the first two minutes but the recovery is significantly delayed. Patients showing such a response are characterized by an extensive network of collateral circulation with a single level of stenosis. In terms of blood oxygenation time-course, we project that these patients would exhibit a relatively short washout time and large upslope among PAD patients but shorter-lasting hyperemia. The type-II response is found in patients with stenoses at two levels and, while the post-occlusive blood flow is elevated compared to baseline, the peak flow rate is significantly delayed. This would correspond to longer washout time and lower upslope. Due to markedly shortened hyperemia or delayed peak blood flow, the overshoot may not offer additional information among PAD patients. Finally, patients with severe PAD exhibit abnormal flow patterns that include loss of pulsatility and significantly reduced peak blood flow (33) and some cases, normal nutritive need cannot be maintained even at rest. None of the PAD subjects in this study belonged to the type-III group and no attempt was made to classify the patients’ hyperemic response in terms of the types described above since information regarding the severity of PAD was not collected for this pilot study. Although we did not measure arterial flow, post-occlusive flow rate was related to washout time and may thus explain why we observed a larger range of washout times among PAD patients compared to YH and AHC (Table 2).

In the present study cuff-compression was chosen over an exercise paradigm for the same reasons stated in the work by Lederman et al (10). Cuff-induced ischemia is straightforward to implement and the examination can be done with the patient immobilized as in a conventional MRI study. Further, reactive hyperemia is induced over the entire lower extremity instead of a specific muscle group. Targeting a specific muscle group may be advantageous if the exact location of the stenosis is known but requires patient compliance, which can affect intra- and intersubject reproducibility. The femoral/popliteal vessel group was chosen over the brachial artery (which has been studied extensively using similar paradigms (34,35)) because PAD predominantly occurs in lower extremity (36) and may translate to greater sensitivity in assessing disease, in particular, possible evaluation of the early stages of atherosclerosis, the ultimate goal of a vascular prognostic tool. In Nishiyama et al’s work significantly greater attenuation in FMD was observed in the popliteal than in the brachial artery in older healthy subjects (71 vs. 38%) (29). When FMD was normalized to shear stress, significant attenuation was no longer observed in the brachial artery, i.e. FMD measurements in the brachial artery may falsely signal vascular dysfunction.

Currently, the differential clinical utility of macro- versus microvascular functional assessment is not fully understood and further studies are required. Recent work by Dhindsa et al suggests only modest correlation between micro- and macrovascular parameters in healthy subjects (ages 19 – 68) (37). However, among stable patients with advanced peripheral vascular disease macrovascular function was a stronger indicator for predicting cardiovascular events (38). On the other hand, findings by Mitchell et al (39) indicate a stronger association between cardiovascular risk factors and shear stress or hyperemic velocity, the latter being a measure of microvascular function. Based on the respective cohorts of (38,39) it has been conjectured that macrovascular function (e.g. FMD) is more sensitive to overt cardiovascular disease whereas evaluation of microvascular function may be more appropriate for assessment of early stages of atherosclerosis (40), e.g. age-related effects in healthy subjects as observed by Nishiyama et al (29).

In this pilot study, only a limited number of subjects were recruited and no angiographic assessment of macrovascular architecture was performed to locate the sites of stenoses and collateral circulation in patients with PAD. Additional physiologic measurements such as quantification of the time-course of the blood flow rate (11,41) during the hyperemic response as well as a systemic assessment of arterial compliance as a part of single integrated examination may provide a more detailed vascular assessment among subjects at risk but without symptoms of PAD. MRI in the form of MR angiography (MRA) has been used routinely for at least a decade in the clinic (having virtually supplanted plain-film angiography) for grading of stenoses and assessment of collateralization (42). However, as a functional tool, MRI is still in the realm of research; such as for quantifying tissue perfusion (43,44). Our study results show MR technology has potential to provide, in a single session, both structural and physiologic parameters to aid in assessing PAD.


The work was supported by the grants NIH T32 EB000814, NIH R21 HL88182 and NIH RO1 HL 075649. The authors gratefully acknowledge Helen Peachey for her excellent work in research coordination.

Grants: NIH T32 EB000814, NIH R21 HL88182 and NIH RO1 HL 075649.

No relationships with industry.




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