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J Clin Microbiol. 2010 March; 48(3): 946–948.
Published online 2010 January 13. doi:  10.1128/JCM.02075-09
PMCID: PMC2832449

16S rRNA Gene Sequencing in Routine Identification of Anaerobic Bacteria Isolated from Blood Cultures[down-pointing small open triangle]

Abstract

A comparison between conventional identification and 16S rRNA gene sequencing of anaerobic bacteria isolated from blood cultures in a routine setting was performed (n = 127). With sequencing, 89% were identified to the species level, versus 52% with conventional identification. The times for identification were 1.5 days and 2.8 days, respectively.

Anaerobic bacteria isolated from blood cultures can originate from multiple locations, e.g., the oral cavity, lower intestine, or female genital tract. If species identification is not performed, important information concerning the recognition of the primary site of infection could be lost and appropriate treatment, e.g., surgical drainage, may be delayed. Also, the diagnosis of recurrent bacteremia will be more difficult and may result in unnecessary reconsideration of the primary site of infection. Conventional phenotypic identification of anaerobic bacteria to the species level can be difficult and laborious in the clinical microbiology laboratory. Commercial phenotypic systems like the Rapid ID 32A or Vitek 2 ANC card (bioMérieux, Marcy-l'Etoile, France) are faster but limited by the taxa included in the system database and, in most cases, only reliable to the genus level (6, 8, 11, 12, 14). In the last few years, the use of 16S rRNA gene sequencing as an alternative method for species identification has increased, while the time and costs (≈$35 to $40) have been reduced (7, 8, 14, 15). The method has been applied for anaerobic bacteria, including isolates from blood cultures, in some studies but not in a routine prospective design (8, 10, 15, 16). To decide in which specific diagnostic situations 16S rRNA gene sequencing could be of benefit in a routine setting with anaerobic bacteria isolated from blood cultures, we performed a comparison between conventional identification and 16S rRNA gene sequencing. The foci of the study were specificity, time, and material cost of the identification.

(The preliminary results from this study were presented at the 48th Annual Interscience Conference on Antimicrobial Agents and Chemotherapy, Washington DC, 25 to 28 October, 2008 [8a].)

During a 1-year period, 16S rRNA gene sequencing was applied to pure culture material from blood cultures (BACTEC Plus anaerobic/F bottle and BACTEC 9240 automated instrument; Becton Dickinson Diagnostic Instrument Systems, Franklin Lakes, NJ) as soon as anaerobic bacteria, including Lactobacillus spp. and Actinomyces spp., were suspected (no corresponding aerobic growth). The MicroSeq 500 system (Perkin-Elmer, Applied Biosystems Division, Foster City, CA) was used for the entire period (8, 11, 14). Conventional anaerobic identification was performed simultaneously, using the identification tables in the Manual of Clinical Microbiology, and was based on Gram stain and wet mount characteristics; hemolysis; fluorescence; susceptibility to metronidazole, vancomycin, kanamycin, colistin, and bile; and biochemical testing, including fermentation of carbohydrates and enzyme tests using commercially available substrates and diagnostic tablets/discs (Statens Serum Institut Diagnostica, Copenhagen, Denmark, and Rosco Diagnostica, Taastrup, Denmark) (13). All isolates were tested, including if the same anaerobic bacteria were isolated from more than one blood culture bottle from the same patient (double isolate). The primary outcome parameter was the specificity of the result, i.e., at what level the method was capable of identifying the isolate (species, genus, descriptive, or no result). Identification to the species level was accepted if there was a ≥99% match with the 16S rRNA gene sequence and at the genus level if there was a ≥97 to 99% match according to CLSI guideline MM18-A (3). Consensus sequences were primarily compared with the MicroSeq ID 2.0 500-bp library. If there were no matches at the species level, the sequences were compared with the EzTaxon server, followed by the NCBI BLAST search engine (2). Secondary conventional identification was done retrospectively in cases of incomplete or conflicting primary conventional identification to determine to what extent conventional identification was in accordance with 16S rRNA gene sequence identification. Secondary conventional identification was supplemental testing and/or retesting of isolates based on the same tests as the primary conventional identification. The secondary outcome parameter was the use of resources, i.e., time and material cost. Standardized time and costs were calculated for 16S rRNA gene sequencing, and the time and material costs of conventional identification were estimated using the worksheets from our local microbiology laboratory database. Bacteroides spp., B. fragilis, Clostridium spp., and Propionibacterium acnes were also evaluated separately, as they usually represent the majority of anaerobic blood culture isolates.

One hundred and twenty-seven isolates (36 double isolates), corresponding to 91 unique isolates, were identified in 84 patients (Table (Table1).1). With 16S rRNA gene sequencing, 113 isolates (89%) were identified to the species level with a ≥99% match and another 7 (5.5%) with a ≥97% match. Three isolates were characterized as Clostridium sp. with a match of <97% but were reclassified after the end of the study to a newly described genus, Robinsoniella (4). Four isolates had no match of ≥97%. With primary conventional identification, 66 (52%) isolates were identified to the species level and another 34 (26.8%) to the genus level, including the three Robinsoniella spp. Fourteen isolates (11%) were only characterized by morphology and Gram stain. Thirteen isolates (10.2%) were misidentified (wrong genus or species) as compared to the results of 16S rRNA gene sequencing. The corresponding results without double isolates (n = 91) were as follows: with 16S rRNA gene sequencing, 87.9% identification to species and 6.6% to genus, and with conventional identification, 51.6% to species and 27.5% to genus, 12.1% descriptive, and 8.8% misidentified.

TABLE 1.
Anaerobic bacteria identified to the species or genus level by 16S rRNA gene sequencing (n = 123)a

After retrospective secondary conventional identification, another 30 isolates were identified to the species level in accordance with the results of 16S rRNA gene sequencing. Only one isolate, which was suspected to be anaerobic, was misclassified. An isolate primarily identified as a Peptostreptococcus sp. by conventional identification was identified as Staphylococcus caprae (usually considered to be facultative) with 16S rRNA gene sequencing (data not included) (13). Time to identification was 1.5 days with 16S rRNA gene sequencing and a mean (range) of 2.8 days (1 to 7 days) with conventional identification. Material costs were $35 to $40 for 16S rRNA gene sequencing and a mean (range) of $10 ($2 to $23) for conventional identification. Only P. acnes could be identified to the species level faster (1.3 days) (100% specificity) and at a lower cost ($4) with conventional identification. Time and material costs were 2.4 days and $12.70 for Bacteroides spp., 2.2 days and $12.70 for B. fragilis, and 3.2 days and $15.70 for Clostridium spp.

The results for identification to the species and genus level by conventional methodology in this study are comparable to those of other studies (15). In our laboratory, species like Sneathia sanguinegens, Solobacterium moorei, and Turicibacter sanguinis would not have been identified to the species level in a reasonable time with conventional methodology. The identification of Sneathia sanguinegens and Solobacterium moorei are good examples of the importance of species identification with prompt recognition of the primary site of infection (17). Sneathia sanguinegens (female genital tract) was isolated from a patient with postpartum fever, and Solobacterium moorei (oral cavity) from a patient with a dental abscess (5, 9). The recognition of contaminants is also important to avoid unnecessary diagnostic testing. Propionibacterium acnes is very often considered a contaminant when isolated from blood cultures, and indeed, in our study, none of the P. acnes isolates were associated with true infection. All other isolates were considered clinically significant pathogens, although Turicibacter sanguinis and Robinsoniella have just recently been described and only a single case report exists on each species (1, 4). Hopefully, species identification with 16S rRNA gene sequencing will further clarify the clinical significance and association between the primary site of infection of both old and new anaerobic bacteria.

In the study, only material costs were calculated and not labor or equipment costs. Conventional identification material costs were lower than those of 16S rRNA gene sequencing. However, the time for identification was longer, with a decrease in specificity. Furthermore, if attempts had been made primarily to identify isolates to the species level with conventional methodology, this would have further prolonged the time, workload, and costs of identification. Undoubtedly, the clinical microbiology specialists used less time per isolate with 16S rRNA gene sequencing, and it is an attractive alternative to conventional identification of anaerobic bacteria from blood cultures. Laboratory technician workload would depend on the number of isolates that were sequenced simultaneously, as more isolates would result in less time per isolate. Only P. acnes was identified faster and at a lower cost with conventional identification. Since the end of the study, the time for 16S rRNA gene sequencing has been reduced with the use of a fast-cycle program, and it can be performed within 8 h if required.

Although material costs are relatively high, 16S rRNA gene sequencing is now applied in our laboratory for anaerobic bacteria from blood cultures and other sterile body sites, e.g., pleural fluid, bones, and joints (except for cases where P. acnes is suspected). However, it should be emphasized that the use of 16S rRNA gene sequencing of anaerobic bacteria from blood cultures and other sterile body sites alone is probably not cost effective if equipment costs are included. Consequently, we run several 16S rRNA gene sequencing tasks (other difficult-to-identify bacteria and culture-negative clinical specimens) simultaneously to maximize cost benefit.

Footnotes

[down-pointing small open triangle]Published ahead of print on 13 January 2010.

REFERENCES

1. Bosshard, P. P., R. Zbinden, and M. Altwegg. 2002. Turicibacter sanguinis gen. nov., sp. nov., a novel anaerobic, Gram-positive bacterium. Int. J. Syst. Evol. Microbiol. 52:1263-1266. [PubMed]
2. Chun, J., J. H. Lee, Y. Jung, M. Kim, S. Kim, B. K. Kim, and Y. W. Lim. 2007. EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int. J. Syst. Evol. Microbiol. 57:2259-2261. [PubMed]
3. Clinical and Laboratory Standards Institute. 2008. Interpretive criteria for identification of bacteria and fungi by DNA target sequencing. Approved standard MM18-A, 1st ed. Clinical and Laboratory Standards Institute, Wayne, PA.
4. Cotta, M. A., T. R. Whitehead, E. Falsen, E. Moore, and P. A. Lawson. 2009. Robinsoniella peoriensis gen. nov., sp. nov., isolated from a swine-manure storage pit and a human clinical source. Int. J. Syst. Evol. Microbiol. 59:150-155. [PubMed]
5. De Martino, S. J., I. Mahoudeau, J. P. Brettes, Y. Piemont, H. Monteil, and B. Jaulhac. 2004. Peripartum bacteremias due to Leptotrichia amnionii and Sneathia sanguinegens, rare causes of fever during and after delivery. J. Clin. Microbiol. 42:5940-5943. [PMC free article] [PubMed]
6. Downes, J., A. King, J. Hardie, and I. Phillips. 1999. Evaluation of the Rapid ID 32A system for identification of anaerobic Gram-negative bacilli, excluding the Bacteroides fragilis group. Clin. Microbiol. Infect. 5:319-326. [PubMed]
7. Drancourt, M., P. Berger, and D. Raoult. 2004. Systematic 16S rRNA gene sequencing of atypical clinical isolates identified 27 new bacterial species associated with humans. J. Clin. Microbiol. 42:2197-2202. [PMC free article] [PubMed]
8. Fontana, C., M. Favaro, M. Pelliccioni, E. S. Pistoia, and C. Favalli. 2005. Use of the MicroSeq 500 16S rRNA gene-based sequencing for identification of bacterial isolates that commercial automated systems failed to identify correctly. J. Clin. Microbiol. 43:615-619. [PMC free article] [PubMed]
8a. Justesen, U. S., M. N. Skov, E. Knudsen, H. M. Holt, P. Søgaard, and T. Justesen. 2008. 16S rRNA gene sequencing in routine identification of anaerobic bacteria isolated from blood cultures, abstr. D-297, p. 221. Abstr. 48th Intersci. Conf. Antimicrob. Agents Chemother., Washington, DC.
9. Kazor, C. E., P. M. Mitchell, A. M. Lee, L. N. Stokes, W. J. Loesche, F. E. Dewhirst, and B. J. Paster. 2003. Diversity of bacterial populations on the tongue dorsa of patients with halitosis and healthy patients. J. Clin. Microbiol. 41:558-563. [PMC free article] [PubMed]
10. Lau, S. K., P. C. Woo, A. M. Fung, K. M. Chan, G. K. Woo, and K. Y. Yuen. 2004. Anaerobic, non-sporulating, Gram-positive bacilli bacteraemia characterized by 16S rRNA gene sequencing. J. Med. Microbiol. 53:1247-1253. [PubMed]
11. Lau, S. K., K. H. Ng, P. C. Woo, K. T. Yip, A. M. Fung, G. K. Woo, K. M. Chan, T. L. Que, and K. Y. Yuen. 2006. Usefulness of the MicroSeq 500 16S rDNA bacterial identification system for identification of anaerobic Gram positive bacilli isolated from blood cultures. J. Clin. Pathol. 59:219-222. [PMC free article] [PubMed]
12. Mory, F., C. Alauzet, C. Matuszeswski, P. Riegel, and A. Lozniewski. 2009. Evaluation of the new Vitek 2 ANC card for identification of medically relevant anaerobic bacteria. J. Clin. Microbiol. 47:1923-1926. [PMC free article] [PubMed]
13. Murray, P. R., E. J. Baron, J. H. Jorgensen, M. L. Landry, and M. A. Pfaller (ed.). 2007. Manual of clinical microbiology, 9th ed. ASM Press, Washington, DC.
14. Petti, C. A., C. R. Polage, and P. Schreckenberger. 2005. The role of 16S rRNA gene sequencing in identification of microorganisms misidentified by conventional methods. J. Clin. Microbiol. 43:6123-6125. [PMC free article] [PubMed]
15. Simmon, K. E., S. Mirrett, L. B. Reller, and C. A. Petti. 2008. Genotypic diversity of anaerobic isolates from bloodstream infections. J. Clin. Microbiol. 46:1596-1601. [PMC free article] [PubMed]
16. Song, Y., C. Liu, M. McTeague, and S. M. Finegold. 2003. 16S ribosomal DNA sequence-based analysis of clinically significant gram-positive anaerobic cocci. J. Clin. Microbiol. 41:1363-1369. [PMC free article] [PubMed]
17. Yuan, S., M. L. Astion, J. Schapiro, and A. P. Limaye. 2005. Clinical impact associated with corrected results in clinical microbiology testing. J. Clin. Microbiol. 43:2188-2193. [PMC free article] [PubMed]

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