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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
From:
Steroids. Author manuscript; available in PMC 2010 April 1.
Published in final edited form as:
Steroids. 2009 Apr–May; 74(4-5): 463–473.
Published online 2009 January 13. doi: 10.1016/j.steroids.2008.12.015

Table 2

Intra- and inter-assay precision of GC-MS analysis of neuroactive steroids.

Neuroactive steroidIntra-assay (% CV)Inter-assay (% CV)

30 pg/ml300 pg/ml3000 pg/ml30 pg/ml300 pg/ml3000 pg/ml
3α,5α-THP11.105.609.6817.026.475.95
3α,5β-THP7.455.219.864.8711.215.11
3α,5α-THDOC6.923.986.885.885.248.52
3α,5β-THDOC4.794.077.353.957.667.69
3α,5α-A7.197.412.9014.728.428.05
3α,5β-A8.476.125.156.546.882.50
3α,5α-A-diol10.295.434.5210.316.303.85
3α,5β-A-diol12.646.475.4716.107.362.53
Pregnenolone7.692.657.4810.516.418.58

For intra-assay variability, five replicates of quality control samples (human charcoal-stripped serum spiked with 30, 300 and 3000 pg/ml) were assayed along with the calibration standards in the same run. Inter-assay variability was obtained by comparing five different analytical runs of quality control samples (human charcoal-stripped serum spiked with 30, 300 and 3000 pg/ml). Coefficients of variation (CV) were calculated as standard deviation*100, divided by the mean.