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BMC Genomics. 2010; 11: 88.
Published online Feb 3, 2010. doi:  10.1186/1471-2164-11-88
PMCID: PMC2829512
Whole-genome sequencing of a laboratory-evolved yeast strain
Carlos L Araya,1 Celia Payen,1 Maitreya J Dunham,1 and Stanley Fieldscorresponding author1,2,3
1Department of Genome Sciences, University of Washington, Box 355065, Seattle, Washington, 98195 USA
2Department of Medicine, University of Washington, Box 355065, Seattle, Washington, 98195 USA
3Howard Hughes Medical Institute, University of Washington, Box 355065, Seattle, Washington, 98195 USA
corresponding authorCorresponding author.
Carlos L Araya: araya/at/u.washington.edu; Celia Payen: payen/at/u.washington.edu; Maitreya J Dunham: maitreya/at/u.washington.edu; Stanley Fields: fields/at/u.washington.edu
Received September 29, 2009; Accepted February 3, 2010.
Background
Experimental evolution of microbial populations provides a unique opportunity to study evolutionary adaptation in response to controlled selective pressures. However, until recently it has been difficult to identify the precise genetic changes underlying adaptation at a genome-wide scale. New DNA sequencing technologies now allow the genome of parental and evolved strains of microorganisms to be rapidly determined.
Results
We sequenced >93.5% of the genome of a laboratory-evolved strain of the yeast Saccharomyces cerevisiae and its ancestor at >28× depth. Both single nucleotide polymorphisms and copy number amplifications were found, with specific gains over array-based methodologies previously used to analyze these genomes. Applying a segmentation algorithm to quantify structural changes, we determined the approximate genomic boundaries of a 5× gene amplification. These boundaries guided the recovery of breakpoint sequences, which provide insights into the nature of a complex genomic rearrangement.
Conclusions
This study suggests that whole-genome sequencing can provide a rapid approach to uncover the genetic basis of evolutionary adaptations, with further applications in the study of laboratory selections and mutagenesis screens. In addition, we show how single-end, short read sequencing data can provide detailed information about structural rearrangements, and generate predictions about the genomic features and processes that underlie genome plasticity.
Despite the wealth of knowledge that comparative genomics has provided about the evolution of past and current life forms, the process of adaptation is still poorly understood. Predictive insight into how adaptation will occur -- which adaptive mutations are likely to arise and fix during selection -- is a daunting challenge. Progress in this area will further efforts to deter cancer progression, the emergence of new pathogens, and antibiotic resistance. As a simple model for analyzing adaptation, experimental evolution of microorganisms provides the unique opportunity to catalog, monitor the dynamics, and measure the reproducibility of adaptation in real time. In the past decade, the characterization of laboratory evolution outcomes beyond phenotypic analysis has been advanced by the use of DNA microarrays. In bacteria and yeast, these approaches have been applied to expose adaptive point mutations and structural rearrangements in response to carbon source replacement as well as carbon source and nutrient limitation [1-4]. Furthermore, studies have begun to unveil the effect of population dynamics on the outcomes of evolution, providing direct evidence of parallel evolution and clonal interference and support for historical contingencies [4-7]. However, these studies are limited in that the precise nature of the adaptive point mutations or large-scale sequence rearrangements in the evolved organisms has not been defined systematically.
In recent years, however, a series of high-throughput DNA sequencing technologies capable of producing gigabases of DNA sequence information in a single experiment have been developed. These technologies are transforming biological research, allowing the rapid identification of genetic variation intrinsic to diseases, behavior and other traits. Indeed, these technologies have been applied to a wide host of biological research problems, from enhancing our understanding of laboratory strains of bacteria by pinpointing suppressor mutations [8] and mutational biases [9], to identifying the genetic variation within all known serotypes of a major viral pathogen [10]. In yeasts, they have been applied to analyze the genetic variation within and between wild and domestic populations [11], the spectrum of spontaneous mutations in Saccharomyces cerevisiae [12] and the adaptive mutations in a Pichia stipilis strain efficient in xylose fermentation [13]. In bacteria, these sequencing technologies have recently been applied to study the rate of evolutionary adaptation over 40,000 generations [14]. Furthermore, they have launched a personal genomics era that is revealing the breadth of genetic variation in the small number of individuals fully sequenced thus far [15-18], and across targeted subgenomic portions in many individuals [19,20], pinpointing disease causative alleles in exomes of affected individuals [21,22].
Here, we use whole-genome sequencing to reveal the repertoire of point mutations and copy number polymorphisms in an evolved S. cerevisiae strain. We sequenced an adapted strain isolated after ~188 generations of a continuous haploid culture under sulfate limitation, and compared this analysis to mutational profiling data obtained using array-based technologies [4]. We generated high-depth sequencing data for the evolved and parental genomes, applied a heuristic approach to uncover single nucleotide polymorphisms (SNPs) and a recursive segmentation algorithm to discover breakpoints and refine copy-number estimates, and devised a general approach to extract precise breakpoint sequence information from single-end short reads. These breakpoints allowed us to delineate the structural rearrangement underlying a 5× copy number amplification of a ~11 kb genomic segment.
We constructed DNA sequencing libraries from genomic DNA of the yeast strain DBY11331, evolved in a sulfate-limited chemostat, and its haploid ancestor strain DBY10147 [4]. We collected 13,555,852 and 13,901,121 single-end, 36 bp reads from the evolved and parent genomes, respectively, on an Illumina Genome Analyzer II platform (using two lanes per strain). More than 75% of the reads (12,274,183 for the evolved genome; 10,441,548 for the parental genome) were aligned to the S. cerevisiae reference genome using Maq [23]. Following quality-filtering, these reads yielded a coverage of ~99.8% of the mappable nuclear genome of both strains with an average read-depth of 35.0× and 28.2× in the evolved and ancestor genomes, respectively [Additional File 1: Supplementary Table S1]. The majority of reads (>80%) had a mapping quality score ≥30.
A heuristic approach to point mutation detection
In sequencing the adapted strain, our aim was to reveal the full complement of genetic adaptations that had occurred during its evolution under sulfate limitation. Copy number variation and point mutation analysis of this strain assessed by use of ORF array and genomic tiling array approaches, respectively, provided a data set against which to compare the efficacy of whole-genome sequencing [4]. To identify point mutations, we examined base calls using criteria that allowed comparisons between nucleotide positions in the evolved and parental short read data sets over the great majority of the genome. We performed SNP calling following two heuristic approaches, one that monitors commonly applied filters for read-depth and various quality scores, and one that is a simplified approach that monitors read-depth and the frequency of base-calls at each position. For the simplified approach, we required at least 6 reads per position with ≥80% concordant SNP calls in the evolved genome, and at least 5 reads per position with ≥70% concordant calls for a different base in the parental genome. This approach recapitulated known features in the evolved genome while maximizing breadth, yielding a high-confidence set of SNPs and that allowed us to examine up to 91% of the mappable bases in the yeast genome. We obtained modest gains in coverage following the simplified SNP calling approach [Additional File 1: Supplementary Tables S2 and S3].
Applying these heuristics, we detected four single-point differences between the laboratory evolved strain and that of its parent: the previously-described point mutations at PBP2 (Y127X), SGF73 (E294X), and UPF3 (G6W) [4], and an additional point mutation intergenic to RRN3 and YPK1 (chr11: 207,469:A>C) which we confirmed by Sanger sequencing (Figure (Figure1).1). The function of the RNA polymerase I-specific transcription initiation factor RRN3 is conserved between yeast and humans [24,25]. In humans, TIF-IA (the RRN3 ortholog) modulates ribosomal gene expression in response to nutrient limitation via the mTOR pathway [26]. The expression of RRN3 is less than half the level in the evolved strain compared to its ancestor, suggesting that this SNP may be regulatory and part of the adaptive response in this lineage. The finding of an additional SNP in the sequencing data but missed by array-based comparative genome sequencing is consistent with the estimated 15% false negative rate for the SNPScanner algorithm [3]. In addition, we searched for small insertions and deletions via gapped alignment of reads unmapped in the Maq alignment, requiring that candidate indel coordinates were sequenced as wildtype in the comparison strain. However, no small insertion or deletion differences between the evolved and parental genomes could be detected [Additional File 1: Supplementary Table S4].
Figure 1
Figure 1
Point mutation discovery in an evolved yeast genome. SNPs that are supported by a read depth of ≥6 and ≥5 in the evolved and ancestor strain sequence data, respectively, are shown. These SNPS are also supported by ≥80% of the base (more ...)
Segmentation-based detection of copy number polymorphisms
Previous analysis of the evolved and parental strains using comparative genome hybridizations on microarrays (array CGH) detected a genomic amplification spanning the high-affinity sulfate transporter SUL1 locus in the genome of the laboratory-evolved strain, providing a 50% fitness advantage relative to the ancestral genome [4]. SUL1 gene amplification was a common adaptation in continuous cultures under sulfate limitation, occurring in 15 of 16 adapted clones from various experimental evolutions [4]. However, the precise breakpoints and nature of the structural rearrangement has not been defined for these amplifications.
We compiled the read-depth data from evolved and parental genome sequencing data sets and calculated the ratio in read-depth for each base across the genome. We used these data to perform circular binary segmentation (CBS) with DNAcopy, a software package designed to partition the genome into segments of equal copy number from high-density array CGH data [27]. DNAcopy formulates the problem of copy-number variation (CNV) analysis as a change-point detection problem, whereby change-points correspond to the genomic locations of copy number transitions. The algorithm searches for change-points by recursively partitioning the genome and performing a maximal t-statistic test between segment ratios, joining segments with comparable ratios. We reasoned that this approach should detect transitions in ratios of sequencing read-depth as well, and applied it to search for such transitions throughout the yeast genome [Additional File 2: Supplementary Figure S1]. Indeed, segmentation of sequencing depth ratios detected an ~11 kb segment harboring SUL1 (chr2: 784,043-795,080 +/- 25 bp) with an estimated 5× amplification (Figure (Figure2A).2A). These breakpoint coordinates are in close proximity to estimated coordinates obtained from tiling array data (chr2: 784,009-795,143 +/- 50 bp) [4], but have narrower uncertainty windows (Figure (Figure2B).2B). The read depth-based copy number analysis yielded a value close to an integer, suggesting it may be a reliable approximation of genomic copy number.
Figure 2
Figure 2
A copy-number polymorphism harboring the SUL1 locus detected via whole genome sequencing. A) Circular binary segmentation of sequencing-depth ratios between evolved and parental genomes using DNAcopy. Gray dots represent the per nucleotide read-depth (more ...)
Breakpoint sequence determination and analysis of rearrangement structure
We sought to pinpoint with single-base resolution the structural rearrangements underlying the SUL1 amplification. To do so, we established a strategy to detect breakpoints in shotgun, single-end, short-read sequencing data. We unearthed breakpoint-spanning reads in the evolved genome sequencing data set by assembling unmapped reads into contigs using Velvet, a short-read de novo assembly algorithm [28]. These contigs were BLAT-aligned to the reference genome sequence in an attempt to detect signatures of novel structures in the genome. This approach yielded three contigs composed of subsequences with alignments to the mappable nuclear genome. Of these contigs, two aligned to sequences within the predicted amplification boundaries [Additional File 1: Supplementary Table S6]. Performing this analysis on the ancestor genome sequencing data yielded two contigs at distinct coordinates [Additional File 1: Supplementary Table S7], from which we estimate the likelihood of the signatures detected in the evolved genome arising independently of the amplification and found within the predicted boundaries to be very low (P = 7.81 × 10-11).
The breakpoint-matching contigs are small (~50 bp) and contain inversions of nearby genomic sequences overlapped by ≤13 bp (Figure (Figure3).3). These breakpoints occur within the CTP1 and PCA1 coding sequences, and would result in 192 and 797 amino acid truncations, respectively. In addition, we found reads spanning the wild-type sequence at these coordinates, indicating that full-length copies of these genes are retained in the genome of the adapted strain. In combination with the inversions in breakpoint-spanning contigs, the observation of reads that conform to the wild-type sequences across the predicted rearrangement boundaries suggests that the 5× amplification spanning chr2: 784,043-795,080 is structured as tandem inversions along the chromosome (Figure (Figure3).3). This arrangement was validated by Southern blot analysis of the region with three different restriction enzymes and two probes [Additional File 2: Supplementary Figure S2]. The fact that at both breakpoints we found short homologous sequences 7-13 bp long overlapping the segmental inversions suggests that short homologous nucleotide tracts may be involved in driving the large structural rearrangements.
Figure 3
Figure 3
Structural rearrangement and breakpoint information in the amplification of the SUL1 locus. Top: Sequence reads from the evolved genome spanning the breakpoints of the chromosome 2 amplification (black and white arrow with red and green ends) are shown (more ...)
Among the contigs with subsequence alignments to the mappable reference genome, the preponderance were composed of mitochondrial sequences [Additional File 1: Supplementary Table S6]. This abundance of contigs of unmapped reads composed of mitochondrial subsequences was recapitulated with the ancestor genome data, but we found little overlap between structures observed in the two mitochondrial genomes [Additional File 1: Supplementary Table S7; Additional File 2: Supplementary Figure S3]. We did not observe copy number gains or losses at these coordinates, which are also covered at high-depth with wild-type sequences. These observations may reflect mitochondrial heteroplasmy in the form of structural rearrangements.
Evolutionary adaptation is a fundamental biological phenomenon with far-reaching implications in cancer, antibiotic resistance, infectious disease management, and ecology. Cataloging the precise genetic changes that underlie an organism's responses to defined selective environments can yield insights not only into the pathways that define fitness in these environments, but also into the breadth and reproducibility of adaptive mutations. The generation of such data requires approaches that can rapidly assay entire genomes with single-base resolution to expose point mutations and at megabase-scale to define structural rearrangements. In this study, we applied whole-genome sequencing to identify the genetic changes in a laboratory-evolved yeast strain, following on similar work in bacteria and phage [14,29-31]. This analysis revealed changes in the genome corresponding to single nucleotide polymorphisms and copy number amplifications, with specific gains over array-based methodologies.
We produced high-depth genome sequences for a yeast strain evolved under nutrient limitation and its parental strain. The genome sequence of the parent was critical in allowing us to pinpoint mutations acquired during experimental evolution, and serves as a template against which to compare the genomes of other descendent strains. We foresee the practice of maintaining high-quality genome sequences for stock strains in laboratories becoming pervasive as it facilitates rapid mutational profiling in laboratory evolution and selection experiments, and is readily applicable to a wide host of organisms and conditions.
We attempted a variety of filtering schemes for SNP-calling, incorporating thresholds for consensus base quality, mapping quality of supporting reads, and consensus quality of adjacent bases. However, these approaches generally reduced the coverage of the genome that could be analyzed or introduced false positives [Additional File 1: Supplementary Table S3]. We therefore adopted heuristics that allowed us to derive a set of high-confidence SNP calls while examining the majority of the haploid genome. This approach detected a novel and potentially regulatory SNP in the evolved genome near the RRN3 locus, encoding a nutrient-responsive transcriptional regulator, which is present at high frequency in the evolving population.
Read-depth information in high-throughput sequencing data yielded clear signals of copy number variation between strains. We applied a segmentation algorithm to refine the copy number estimate for the SUL1 amplification on chromosome 2. Following detection of copy number polymorphisms, we developed a general approach for detecting breakpoint sequences from single-end, short read sequencing data. Applied to the data of the genome of the evolved strain, this approach yielded upstream and downstream breakpoint predictions with single-base resolution for the SUL1 amplification. Using the derived breakpoint sequences, we predicted a ~55 kb genomic rearrangement supporting the copy number amplification of the SUL1 locus, and we validated this rearrangement experimentally [Additional File 2: Supplementary Figure S2]. The breakpoint sequences observed share the structural features of palindrome formation triggered by double-strand breaks near short inverted segments or by incorrect cleavage by a Holliday junction resolvase at an inverted repeat-mediated cruciform structure, and subsequent ligation of hairpins [32]. Narayanan et al. [33] have previously established this later mechanism in the development of inverted head-to-tail clusters of CUP1 and SFA1 between inserted Alu repeats in yeast centromeres. Thus, the observed breakpoint sequences in reads that match to two genomic locations provide insights into the structure of the segmental duplications and further evidence for the involvement of regions of micro-homology (≤15 bp) in driving large-scale genomic rearrangements [34].
In this study, we demonstrate the ability of high-throughput sequencing to catalog the genetic changes underlying adaptation in a yeast strain evolved in a sulfate-limited environment. We generated high-depth genome sequences for evolved and parental yeast strains. We describe approaches effective at identifying single-nucleotide polymorphisms, as well as detecting the location of and quantifying copy number changes in the evolved genome with respect to that of its parental strain. Furthermore, we developed a general approach for detecting breakpoint sequences in single-end, short read sequencing data. This approach yielded breakpoint predictions with single base resolution for a ~11 kb amplification harboring the SUL1 gene, providing insights into the mechanisms that may have facilitated the expansion underlying this adaptive rearrangement. As such, this study suggests that the combination of whole-genome sequencing and experimental evolution is a powerful approach to study the features that restrain and promote genomic plasticity, defining possible routes of adaptation and outcomes of evolution. In addition, such studies should yield valuable functional information on the relationship between fitness and adaptations at both the single-gene and genomic levels [35]. Scaling these studies to population-level analysis will allow us to frame this knowledge within the context of population forces, which may yield new insights into evolutionary dynamics.
DBY11331 (referred to as S2c1 in Gresham et al.) was isolated after ~188 generations of a sulfate-limited continuous culture seeded with the prototrophic haploid S. cerevisiae strain DBY10147 (MATα, URA3), as previously described [4]. Illumina sequencing libraries were constructed from DBY11331 and DBY10147 genomic DNA following standard procedures and published recommendations [36]. Briefly, 10 μg of yeast genomic DNA were sonicated to fragment sizes below 2000 bp, concentrated and end-repaired using the End-It DNA repair kit (EPICENTRE Biotechnologies). End-repaired DNA was A-tailed with GoTaq DNA polymerase (Promega) and ligated to Illumina adapters (QuickLigase, NEB). Ligation products between 300-400 bp were excised from a 6% polyacrylamide gel, eluted and ethanol precipitated. Fragment libraries were PCR amplified, cleaned following AMPure (Agencourt) and Qiaquick PCR clean-up procedures, and submitted for sequencing. We prepared two such libraries for each strain.
We collected 13,555,852 and 13,901,121 single-end, 36 bp, quality-filtered reads from DBY11331 and DBY10147, respectively, using the Illumina Genome Analyzer II platform. Reads were aligned to the UCSC sacCer1 reference sequence using Maq [23] with default parameters for single-end reads (12,274,183 evolved strain and 10,441,548 parental strain reads), to a coverage of ≥99.8%. We filtered reads with low mapping quality (score <10) and obtained a final coverage of ≥93.5% with an average read-depth of 35× and 28× in the non-gap regions of the evolved and parental genomes, respectively [Additional File 1: Supplementary Table S1].
For SNP-calling, we settled on a approach that required a nucleotide read depth ≥6× per position, with ≥80% base-calls supporting a SNP in the evolved genome data and ≥5× read depth, with ≥70% base-calls supporting a different base in the parental genome data. These nucleotide read depth thresholds allowed us to examine 90.99% of the mappable genome for SNPs [Additional File 1: Supplementary Table S2]. A parallel analysis relying on consensus base quality, quality of adjacent bases, and read mapping quality filters yielded similar SNP calls, but the fraction of the genome compliant with the analysis criteria was slightly reduced [Additional File 1: Supplementary Table S3].
We searched for small insertions and deletions by performing gapped alignment (BLAT) of the Maq-unmapped reads to the reference genome and recovering coordinates at which multiple unmapped reads show a bipartite alignment -an alignment to flanking sequences- as the best alignment. Candidate indel coordinates were reduced to sets specific to the evolved or ancestor genome. These strain-specific, candidate indels were then refined to maintain sites at which wild-type sequences are not observed in the Maq-alignment in the corresponding genome sequencing data, but are obtained in the comparison strain [Additional File 1: Supplementary Table S4].
To detect copy-number polymorphisms (CNPs), we averaged the per-nucleotide read depth data across 25 bp bins across the unique nuclear genome and normalized by the total nuclear bases acquired. For each bin, the log2-ratio in read depth between the evolved and parental data was calculated. Circular binary segmentation was applied on the ratios using DNAcopy [27], available as an R package, to partition the genome into regions of equal copy number. Segments were smoothed by removing changes < 3 standard deviations, and only those spanning ≥1000 bp were considered for further analysis. We used only one lane of data for each strain for copy number analysis (NCBI Sequence Read Archive accessions SRX014130 and SRX014132).
Breakpoint sequences for the SUL1 amplification were discovered as follows: Unmapped reads from the evolved genome data were assembled into contigs using Velvet, a de novo assembler for short-reads [28]. Contigs of unmapped reads were BLAT-aligned against the reference genome sequence requiring ≥90% un-gapped sequence identity. Alignments were filtered to remove contigs in which shared identity to a single genomic region spans the length of the contig, and contigs whose ends fall within the unmappable portions of the genome. In addition, we filtered contigs to remove those for which the subsequence alignments do not cover ≥90% of the contig. From this filtered group (11 contigs), seven contigs are composed of mitochondrial subsequences, three of nuclear subsequences, and one shares sequence identity to nuclear and mitochondrial sequences [Additional File 1: Supplementary Table S6]. The two contigs aligned to amplification boundary coordinates were selected as candidate breakpoint sequences for the SUL1 amplification and examined in detail. We estimated the probability of these candidate breakpoint contigs arising independently of the amplification by analyzing contigs of unmapped reads derived from the ancestor genome data. Briefly, contigs of ancestor genome unmapped reads were assembled and aligned to the reference genome sequence. This yielded two contigs of unmapped reads composed of sequences with alignments to the mappable nuclear genome coordinates [Additional File 1: Supplementary Table S7]. The probability of observing such contigs was then calculated per mappable base in the nuclear genome. We limit this estimate to the nuclear genome to account for differences in the read-depth between the nuclear and mitochondrial genomes.
For Southern blot analysis, genomic DNA was digested overnight with BamHI, EcoRV or PstI (New England Biolabs). Samples were subjected to electrophoresis through 0.6% w/v agarose in 1× TBE overnight at 33 V, visualized after ethidium bromide staining and transferred to a GeneScreen™ hybridization transfer membrane (PerkinEilmer) in 10× SSC. Hybridization was performed at 65°C for approximately 20 h with 32P-labeled "SUL1" and "BamHI" probes constructed by PCR [Additional File 1: Supplementary Table S5].
Tiling array SNP analysis and ORF array CGH data were obtained as previously described [4].
Data deposition
Data are archived at NCBI Sequence Read Archive (SRA) under accession SRP001478.
Abbreviations
(SNP): Single-nucleotide polymorphism; (CNP): copy-number polymorphism; (CNV): copy-number variation; (CGH): comparative genome hybridization.
Authors' contributions
CA, MD and SF conceived of and designed the experiments and wrote the manuscript. CA performed all sequencing experiments and computational analysis. CP performed Southern blot experiments. All authors read and approved the final manuscript.
Supplementary Material
Additional file 1
Supplementary Tables. This file contains the supplementary tables with information on sequencing and mapping statistics, SNP calling, small insertion and deletion screening, Southern blot probe specifications, and alignment information for contigs of unmapped reads.
Additional file 2
Supplementary Figures. This file contains supplementary figures including a per-chromosome view of the segmentation analysis, Southern blot experimental results, and views of alignments for contigs of unmapped reads.
Acknowledgements
We thank Choli Lee and Amber Leahey for processing sequencing samples, Jay Shendure for performing image analysis for read base calling, Emily Turner for helpful guidance in library construction, Jay Hesselberth for assistance in computational analysis, and Bonita Brewer for helpful comments in the preparation of this manuscript. S.F. is an investigator of the Howard Hughes Medical Institute.
  • Dunham MJ, Badrane H, Ferea T, Adams J, Brown PO, Rosenzweig F, Botstein D. Characteristic genome rearrangements in experimental evolution of Saccharomyces cerevisiae. Proc Natl Acad Sci. 2002;99:16144–16149. doi: 10.1073/pnas.242624799. [PubMed] [Cross Ref]
  • Herring C, Raghunathan A, Honisch C, Patel T, Applebee M, Joyce A, Albert T, Blattner F, Boom D Van Den, Cantor C, Palsson B. Comparative genome sequencing of Escherichia coli allows observation of bacterial evolution on a laboratory timescale. Nat Genet. 2006;38:1406–1412. doi: 10.1038/ng1906. [PubMed] [Cross Ref]
  • Gresham D, Ruderfer DM, Pratt SC, Schacherer J, Dunham MJ, Botstein D, Kruglyak L. Genome-wide detection of polymorphisms at nucleotide resolution with a single DNA microarray. Science. 2006;311:1932–1936. doi: 10.1126/science.1123726. [PubMed] [Cross Ref]
  • Gresham D, Desai M, Tucker C, Jenq H, Pai D, Ward A, Desevo C, Botstein D, Dunham M. The repertoire and dynamics of evolutionary adaptations to controlled nutrient-limited environments in yeast. PLoS Genet. 2008;4:e1000303. doi: 10.1371/journal.pgen.1000303. [PMC free article] [PubMed] [Cross Ref]
  • Segre AV, Murray AW, Leu JY. High-resolution mutation mapping reveals parallel experimental evolution in yeast. PLoS Biol. 2006;4:e256. doi: 10.1371/journal.pbio.0040256. [PMC free article] [PubMed] [Cross Ref]
  • Blount ZD, Borland CZ, Lenski R. Historical contingency and the evolution of a key innovation in an experimental population of Escherichia coli. Proc Natl Acad Sci. 2008;105:7899–7906. doi: 10.1073/pnas.0803151105. [PubMed] [Cross Ref]
  • Kao K, Sherlock G. Molecular characterization of clonal interference during adaptive evolution in asexual populations of Saccharomyces cerevisiae. Nat Genet. 2008;40:1499–1504. doi: 10.1038/ng.280. [PMC free article] [PubMed] [Cross Ref]
  • Srivatsan A, Han Y, Peng J, Tehranchi A, Gibbs R, Wang JD, Chen R. High-precision, whole-genome sequencing of laboratory strains facilitates genetic studies. PLoS Genet. 2008;4:e1000139. doi: 10.1371/journal.pgen.1000139. [PMC free article] [PubMed] [Cross Ref]
  • Lind PA, Andersson DI. Whole-genome mutational biases in bacteria. Proc Natl Acad Sci. 2008;105:17878–17883. doi: 10.1073/pnas.0804445105. [PubMed] [Cross Ref]
  • Palmenberg AC, Spiro D, Kuzmickas R, Wang S, Djikeng A, Rathe JA, Fraser-Liggett CM, Liggett SB. Sequencing and analyses of all known human rhinovirus genomes reveal structure and evolution. Science. 2009;324:55–59. doi: 10.1126/science.1165557. [PubMed] [Cross Ref]
  • Liti G, Carter DM, Moses AM, Warringer J, Parts L, James SA, Davey RP, Roberts IN, Burt A, Koufopanou V. et al. Population genomics of domestic and wild yeasts. Nature. 2009;458:337–341. doi: 10.1038/nature07743. [PMC free article] [PubMed] [Cross Ref]
  • Lynch M, Sung W, Morris K, Coffey N, Landry CR, Dopman EB, Dickinson WJ, Okamoto K, Kulkarni S, Hartl DL, Thomas WK. A genome-wide view of the spectrum of spontaneous mutations in yeast. Proc Natl Acad Sci. 2008;105:9272–9277. doi: 10.1073/pnas.0803466105. [PubMed] [Cross Ref]
  • Smith DR, Quinlan AR, Peckham HE, Makowsky K. Rapid whole-genome mutational profiling using next-generation sequencing technologies. Genome Res. 2008;18:1638–1642. doi: 10.1101/gr.077776.108. [PubMed] [Cross Ref]
  • Barrick J, Yu D, Yoon S, Jeong H, Oh T, Schneider D, Lenski R, Kim J. Genome evolution and adaptation in a long-term experiment with Escherichia coli. Nature. 2009;461:1243–1247. doi: 10.1038/nature08480. [PubMed] [Cross Ref]
  • Wheeler DA, Srinivasan M, Egholm M, Shen, Chen, McGuire, He W, Chen YJ, Makhijani, Roth. et al. The complete genome of an individual by massively parallel DNA sequencing. Nature. 2008;452:872–876. doi: 10.1038/nature06884. [PubMed] [Cross Ref]
  • Bentley DR, Balasubramanian S, Swerdlow H, Smith G, Milton J, Brown C, Hall K, Evers D, Barnes C, Bignell H. et al. Accurate whole human genome sequencing using reversible terminator chemistry. Nature. 2008;456:53–59. doi: 10.1038/nature07517. [PMC free article] [PubMed] [Cross Ref]
  • Wang J, Wang W, Li R, Li Y, Tian G, Goodman L, Fan W, Zhang J, Li J, Guo Y. et al. The diploid genome sequence of an Asian individual. Nature. 2008;456:60–65. doi: 10.1038/nature07484. [PMC free article] [PubMed] [Cross Ref]
  • Ahn SM, Kim T, Lee S, Kim D, Ghang H, Kim D, Kim B, Kim S, Kim W, Kim C. et al. The first Korean genome sequence and analysis: Full genome sequencing for a socio-ethnic group. Genome Res. 2009;19:1622–1629. doi: 10.1101/gr.092197.109. [PubMed] [Cross Ref]
  • Tarpey PS, Smith R, Pleasance E, Whibley A, Edkins S, Hardy C, O'Meara S, Latimer C, Dicks E, Menzies A. et al. A systematic, large-scale resequencing screen of X-chromosome coding exons in mental retardation. Nat Genet. 2009;41:535–543. doi: 10.1038/ng.367. [PMC free article] [PubMed] [Cross Ref]
  • Turner EH, Lee C, Ng SB, Nickerson DA, Shendure J. Massively parallel exon capture and library-free resequencing across 16 genomes. Nat Meth. 2009;6:315–316. doi: 10.1038/nmeth.f.248. [PMC free article] [PubMed] [Cross Ref]
  • Ng SB, Turner EH, Robertson P, Flygare SD, Bigham AW, Lee C, Shaffer T, Wong M, Bhattacharjee A, Eichler E. et al. Targeted capture and massively parallel sequencing of 12 human exomes. Nature. 2009;461:272–276. doi: 10.1038/nature08250. [PMC free article] [PubMed] [Cross Ref]
  • Ng SB, Buckingham KJ, Lee C, Bigham AW, Tabor HK, Dent KM, Huff CD, Shannon PT, Jabs EW, Nickerson DA. et al. Exome sequencing identifies the cause of a mendelian disorder. Nat Genet. 2009;42:30–35. doi: 10.1038/ng.499. [PMC free article] [PubMed] [Cross Ref]
  • Li H, Ruan J, Durbin R. Mapping short DNA sequencing reads and calling variants using mapping quality scores. Genome Res. 2008;18:1851–1858. doi: 10.1101/gr.078212.108. [PubMed] [Cross Ref]
  • Bodem J, Dobreva G, Hoffmann-Rohrer U, Iben S, Zentgraf H, Delius H, Vingron M, Grummt I. TIF-IA, the factor mediating growth-dependent control of ribosomal RNA synthesis, is the mammalian homolog of yeast Rrn3p. EMBO Rep. 2000;1:171–175. doi: 10.1093/embo-reports/kvd032. [PubMed] [Cross Ref]
  • Moorefield B, Greene EA, Reeder RH. RNA polymerase I transcription factor Rrn3 is functionally conserved between yeast and human. Proc Natl Acad Sci. 2000;97:4724–4729. doi: 10.1073/pnas.080063997. [PubMed] [Cross Ref]
  • Mayer C, Zhao J, Yuan X, Grummt I. mTOR-dependent activation of the transcription factor TIF-IA links rRNA synthesis to nutrient availability. Genes & Dev. 2004;18:423–434. [PubMed]
  • Venkatraman ES, Olshen AB. A faster circular binary segmentation algorithm for the analysis of array CGH data. Bioinformatics. 2007;23:657–663. doi: 10.1093/bioinformatics/btl646. [PubMed] [Cross Ref]
  • Zerbino DR, Birney E. Velvet: algorithms for de novo short read assembly using de Bruijn graphs. Genome Res. 2008;18:821–829. doi: 10.1101/gr.074492.107. [PubMed] [Cross Ref]
  • Shendure J, Porreca GJ, Reppas NB, Lin X, McCutcheon JP, Rosenbaum AM, Wang MD, Zhang K, Mitra RD, Church GM. Accurate multiplex polony sequencing of an evolved bacterial genome. Science. 2005;309:1728–1732. doi: 10.1126/science.1117389. [PubMed] [Cross Ref]
  • Pepin KM, Wichman HA. Experimental evolution and genome sequencing reveal variation in levels of clonal interference in large populations of bacteriophage phiX174. BMC Evol Biol. 2008;8:85. doi: 10.1186/1471-2148-8-85. [PMC free article] [PubMed] [Cross Ref]
  • Beaumont H, Gallie J, Kost C, Ferguson G. Experimental evolution of bet hedging. Nature. 2009;462:90–93. doi: 10.1038/nature08504. [PubMed] [Cross Ref]
  • Haber JE, Debatisse M. Gene amplification: yeast takes a turn. Cell. 2006;125:1237–1240. doi: 10.1016/j.cell.2006.06.012. [PubMed] [Cross Ref]
  • Narayanan V, Mieczkowski PA, Kim HM, Petes TD, Lobachev KS. The pattern of gene amplification is determined by the chromosomal location of hairpin-capped breaks. Cell. 2006;125:1283–1296. doi: 10.1016/j.cell.2006.04.042. [PubMed] [Cross Ref]
  • Payen C, Koszul R, Dujon B, Fischer G, Haber JE. Segmental Duplications Arise from Pol32-Dependent Repair of Broken Forks through Two Alternative Replication-Based Mechanisms. PLoS Genet. 2008;4:e1000175. doi: 10.1371/journal.pgen.1000175. [PMC free article] [PubMed] [Cross Ref]
  • Hegreness M, Kishony R. Analysis of genetic systems using experimental evolution and whole-genome sequencing. Genome Biol. 2007;8:201. doi: 10.1186/gb-2007-8-9-r201. [PMC free article] [PubMed] [Cross Ref]
  • Quail MA, Kozarewa I, Smith F, Scally A, Stephens PJ, Durbin R, Swerdlow H, Turner DJ. A large genome center's improvements to the Illumina sequencing system. Nat Meth. 2008;5:1005–1010. doi: 10.1038/nmeth.1270. [PMC free article] [PubMed] [Cross Ref]
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