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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Neurosci. Author manuscript; available in PMC 2010 July 13.
Published in final edited form as:
PMCID: PMC2826135
NIHMSID: NIHMS172344

Blue-yellow opponency in primate S cone photoreceptors

Abstract

The neural coding of human color vision begins in the retina. The outputs of long (L), middle (M) and short (S) wavelength sensitive cone photoreceptors combine antagonistically to produce “red-green” and “blue-yellow” spectrally opponent signals (Hering, 1878; Hurvich and Jameson, 1957). Spectral opponency is well-established in primate retinal ganglion cells (Reid and Shapley, 1992; Dacey and Lee, 1994; Dacey, et al., 1996) but the retinal circuitry creating the opponency remains uncertain. Here we find, from whole cell recordings of photoreceptors in macaque monkey, that “blue-yellow” opponency is already present in the center-surround receptive fields of S cones. The inward current evoked by blue light derives from phototransduction within the outer segment of the S cone. The outward current evoked by yellow light is caused by feedback from horizontal cells that are driven by surrounding L and M cones. Stimulation of the surround modulates calcium conductance in the center S cone.

Keywords: color vision, photoreceptors, retina, primate, monkey, Macaque

Introduction

In the primate retina the L and M cones comprise about 90% of the total cone population (Ahnelt et al., 1987; Curcio, et al., 1991) and are thus relatively accessible for physiological study. L and M cones are electrically coupled to each other and to rods via gap junctions over distances of ~10-20 μm (Hornstein et al., 2004; Hornstein et al., 2005). They also receive inhibitory signals from neighboring L and M cones over much larger distances via an interneuron, the H1 horizontal cell (Dacey et al., 1996; Packer and Dacey, 2002; Verweij et al., 2003). How these signals are transmitted from horizontal cells to cones is controversial (Hirasawa and Kaneko, 2003; Fahrenfort et al., 2009; Davenport, et al., 2008), but the end target is the modulation of calcium channels in the cone synapse (Verweij et al., 1996; Verweij et al., 2003).

In contrast, primate S cones comprise only about 10% of the cones and thus physiological recordings from S cones are rare (Baylor et al., 1987; Schnapf et al., 1990; Hornstein et al., 2004). Anatomically, S cones contact both H2 horizontal cells and rods (Ahnelt et al., 1990; Dacey et al., 1996; Goodchild et al., 1996). However it’s not known if these contacts provide input to S cones.

Human psychophysical studies indicate that blue-yellow opponency is created by neurons that receive opponent signals from S cones vs. (L+M) cones (Hurvich and Jameson, 1957; Krauskopf et al., 1982). One such neuron is the small-bistratified ganglion cell (Dacey and Lee, 1994). The dendrites of the small bistratified cell stratify in both the ON and OFF portions of the inner plexiform layer, suggesting that the blue-ON and yellow-OFF responses reflect synaptic input from S-ON bipolar cells and (L+M)-OFF bipolar cells respectively (Dacey and Lee, 1994; Calkins et al., 1998). However in a recent study (Field et al., 2007), both the blue and yellow responses were abolished when the ON pathway was selectively blocked by L-AP4, suggesting that both the blue and the yellow signals arrive via the S-ON bipolar cell. Since this bipolar cell contacts S but not L or M cones, it was inferred that the (L+M) signal was already present in S cones, and that it arose from horizontal cell feedback to S cones. By contrast, a later study found that L-AP4 selectively blocked the S-ON and spared the L+M-OFF responses, consistent with the (L+M) signal arriving via the (L+M)-OFF bipolar cell (Crook et al., 2009). Thus it remains uncertain whether blue-yellow opponency in the retina arises from horizontal cell feedback to cones, from the convergence of ON- and OFF-bipolar cell inputs to the ganglion cell, or from some combination of both.

To address this question we asked whether primate S cones photoreceptors showed evidence of center-surround organization as previously found for L and M cones, and if so whether spectral opponency was present. To characterize their spatial and spectral receptive field properties we selectively targeted the minority S cones for whole-cell voltage clamp recordings (Verweij et al., 2003) in isolated macaque monkey retina.

Materials and Methods

Eyes from Old-World monkeys (Macaca nemestrina, Macaca fascicularis, and Papio cynocephalus Anubis) were obtained from tissue distribution programs at the Washington National Primate Research Center and the California National Primate Research Center. The combined retina-choroid-pigment epithelium was dissected and incubated in the dark for at least 1 hour in bicarbonate-buffered Ames medium at 36°C. Small portions of retina were isolated and placed photoreceptor-side up in a recording chamber superfused with Ames medium. Single cones were voltage-clamped with whole-cell patch electrodes (Verweij et al., 2003). In most experiments the electrode solution contained (in mM) K-gluconate (99), KCl (32), MgCl2 (4.5), HEPES (10), ATP-Na2 (3), GTP-Na3 (1), and BAPTA-K4 (5). When measuring calcium currents, K-gluconate was replaced with Cs-gluconate (99), and KCl was replaced with CsCl (22) and TEA-Cl (10). Voltages were corrected for electrode junction potential and series resistance. Light stimuli were created with a 4 channel light emitting diode (LED) based stimulator (Pokorny et al., 2004) or a 3-primary digital light projector (Crook et al., 2009). Unless indicated otherwise, stimulus radius was 1 mm. Descriptions of the optics, cone-isolating stimuli, and calculations of cone quantal catch rates are given elsewhere (Crook et al., 2009).

Results

Cone identification and “blue” receptive field center

Anatomical features were used to specifically target S cones for recording. The maximal inner diameter of S cone inner segments was smaller than that of L and M cones, and S cones tended to disrupt the regular spatial array of cones (Ahnelt et al., 1990; Curcio et al., 1991) (Fig. 1A, arrow). Cone identity was determined from the relative response amplitudes to light pulses from red, green, cyan, and blue LEDs and further confirmed with stimuli that independently modulated the photon catch rate of the S, M or L photopigment (Baylor et al., 1987; Crook et al., 2009) (Fig. 1B). Consistent with previous observations (Hornstein et al., 2004; Li and DeVries, 2004), the spectral sensitivity of S cones showed no evidence for electrical coupling to neighboring L and M cones (Fig. 1B).

Figure 1
S cone identification. A, Infrared image of retina focused on rod (small) and cone (large) inner segments. Arrow indicates S cone. B, Membrane current response to cone-isolating stimuli. Colored lines plot photoisomerization/s (R*/s) in L (red), M (green) ...

To determine if S cones were electrically coupled to neighboring S cones, the receptive field size was measured with S photopigment-isolating gratings that were modulated sinusoidally in space and drifted across the retina at 2 Hz. Assuming response linearity, peak response amplitude (A) as a function of spatial frequency (f) is given by the 2-dimensional Fourier transform of the receptive field sensitivity profile, which simplifies to a zero order Hankel transform for a radially symmetric receptive field (Bracewell, 1999). For a receptive field profile that declines exponentially with radial distance from the center

A(f)=k[(1/λ)2+(2πf)2]3/2
(1)

where λ is the exponential length constant and k is a scalar. In 13 S cones the best fitting λ was 5.3 ± 3.4 μm (mean ± SD). The choice of an exponential function was somewhat arbitrary; similarly good fits were obtained with a Gaussian function. The receptive field sizes are larger than would be expected for a single cone, however spatial resolution of the field size appears to have been limited by our optics as evidenced by the subset of 3 cones where image focus was optimized with a high numeric aperture water-immersion lens. In these cones λ ranged from 1.9 - 4.1 μm (Fig. 1C). Nevertheless given that all S cones were recorded in far peripheral retina where their average separation is 50-70 μm (Curcio et al., 1991), the receptive field sizes from all 13 cones indicate that there is no significant electrical coupling between S cones.

“Yellow” inhibitory surround arises from horizontal cell feedback

The center-surround opponency of cone receptive fields results from feedback inhibition from horizontal cells (Baylor et al., 1971; Verweij et al., 2003). In primate L and M cones, the feedback signal is enhanced by holding the membrane potential at -40 mV and by including elevated chloride (ECl = -30 mV) and calcium chelator BAPTA in the patch solution (Verweij et al., 2003). Using these conditions we observed clear blue-center yellow-surround opponency in S cones. Blue light that strongly activated S cone photopigment evoked outward currents, while yellow light that preferentially activated surrounding L and M cones evoked inward currents (Fig. 2A) (n=35). Measurements of long-wavelength sensitivity of the inward current confirmed that both L and M cones contribute to the opponent surround of S cones (n=7, data not shown). The only known pathway by which L and M cones could transmit an opponent signal to S cones is via H2 horizontal cells (Dacey et al., 1996). Since H2 horizontal cells receive input from all three cone spectral types (Dacey et al., 1996), the opponent surround of an S cone would be expected to have an S cone component as well. While none was observed, the lack of a demonstrable contribution from S cones in the surround may simply reflect our inability to resolve a small inhibitory S cone signal when superimposed on a large excitatory S cone phototransduction signal.

Figure 2
S cone chromatic opponency is created by horizontal cell feedback. A, Short wavelength light (blue) evokes outward current; long wavelength light (yellow) evokes inward current. B, Inward current is blocked by 20 μM CNQX. A-B, Membrane potential ...

If the opponent surround response is mediated by horizontal cells, it should be blocked by CNQX, an antagonist that blocks glutamate receptors on horizontal cells (Haverkamp et al., 2001a, b; Verweij et al., 2003). Figure 2 shows that the surround response was indeed reversibly blocked by superfusion of 20 μM CNQX (Fig 2B, n=2), consistent with horizontal cells mediating the response.

Calcium dependence of the inhibitory surround

The voltage dependence of the surround response (Fig. 3) indicates that surround stimulation modulates calcium conductance in S cones. To measure the calcium current, voltage-activated potassium and Ih channels and calcium-activated chloride channels were blocked by including cesium, TEA, and BAPTA in the electrode solution (Yagi and MacLeish, 1994). The remaining voltage-activated current was inward (Fig. 3A-B) and displayed the kinetics and voltage-sensitivity of L-type calcium channels (Barnes and Hille, 1989; Yagi and MacLeish, 1994; Verweij et al., 1996). Measureable currents were observed at membrane potentials positive to -55 mV. The current was sustained for membrane potentials up to -15 mV; larger depolarizations evoked inactivation (data not shown). Stimulation of the surround with yellow light increased the maximal inward current and shifted its voltage activation range to more negative potentials (Fig. 3A-B). The surround-evoked enhancement of the current was greatest at a membrane potential of ~ -40 mV (Fig. 3C) (n=6). The voltage-dependence of the surround was tested a second way: the amplitude of the response to a yellow light flash was measured as a function of steady state membrane potential. Response amplitude was maximal at -42 ± 4 mV (mean ± SD; n = 41). Thus surround activation was optimal at a voltage close to the physiological dark resting potential of ~-45 mV. These data are consistent with the idea that the surround-evoked inward current reflects the modulation of a voltage-activated calcium current (Verweij et al., 1996).

Figure 3
Opponent surround activates calcium currents. A, Voltage-activated calcium currents increased in steady yellow light. Membrane potential stepped from −70 mV to between -85 and -5 mV. Responses shown for steps to between -50 and -30 mV. Voltage ...

In addition to its modulation by horizontal cell feedback, calcium conductance in the cone synapse has also been shown in ground squirrel to be modulated by protons that are released via exocytosis of the cone’s own synaptic vesicles (DeVries, 2001). The light modulation of the calcium conductance that we observe here however cannot be accounted for by such a mechanism because the cone was voltage-clamped and hence voltage-dependent exocytosis was held constant across dark/light conditions. This does not however rule out an in vivo role for local vesicle-based modulation of calcium conductance in the S cone synapse.

Spatial properties of inhibitory surround

The spatial extent of the opponent surround was examined with annular stimuli. The annulus was composed of either yellow light that preferentially activated L and M photopigment, or an L+M cone-isolating stimulus. The annulus inner radius rI varied while the outer radius rO was fixed at 0.5-1 mm. In most cells response amplitude varied non-monotonically with radius (Fig. 4), indicating that the annulus not only stimulated the surround but also weakly stimulated a “center” mechanism whose polarity was opposite that of the surround. Assuming that center and surround sensitivity profiles decline exponentially with distance from the center, the peak response amplitude R is given by:

R=kS[erI/λS(1+rIλS)erO/λS(1+rOλS)]kC[erI/λC(1+rIλC)erO/λC(1+rOλC)]
(2)

where λC and λS are exponential length constants for the center and surround, and kC and kS are scalars. The best-fitting exponential length constant λS for the surround component was 131 ± 50 μm (mean ± SD; n = 12). This is close to the value of 144 μm found to describe the exponential sensitivity profile of a macaque H2 horizontal cell (Dacey, 2000). Hence, the spatial extent of the opponent surround we observed here is consistent with feedback inhibition from horizontal cells.

Figure 4
Size of opponent surround. A, Responses to flashed annuli; inner radius 25-500 μm, outer radius 1 mm. Flash monitor above current traces. B, Peak response amplitude vs. inner radius from 4 cones. Annular flash: long wavelength flash from darkness ...

The weak center component of the S cone receptive field (λC = 31 ± 13 μm) could result from either direct stimulation of the S cone itself or rod input through gap junctions (Ahnelt et al., 1990; Schneeweis and Schnapf, 1995). Direct stimulation of the S cone is unlikely however because the center component was present with (L+M) stimuli that modulated rod but not S cone photopigment (● in Fig. 4). That this putative rod input was not observed with the S cone grating stimulus (Fig. 1) probably reflects the fact that the rods were more light adapted, the stimulus contrast was lower for rods, and the grating strongly activated the S cone outer segment directly.

Discussion

We have determined that horizontal cell feedback to S cones creates the first stage of blue-yellow opponency in the primate retina. The maximal response amplitudes of the S cone center and (L+M) cone surround were roughly comparable when recorded at a membrane potential of -40 mV (Fig. 2A). However in the physiological range between -45 and -70 mV, center responses remained largely unchanged (data not shown) (Miller and Korenbrot, 1993) but surround responses were significantly reduced (Fig. 3C). Nonetheless, even with this reduced size the surround is expected to be a potent modulator of synaptic transmission because it directly modulates calcium current at the synapse. In contrast, only a small fraction of the current evoked by center stimulation is carried by calcium. Consequently, although surround response is proportionately small under physiological conditions, it will have a disproportionately large effect on the synaptic transmission of cone signals.

The calcium-dependence of the opponent yellow surround may help explain the paradoxical psychophysical finding that yellow light can increase sensitivity to blue light- a flash of blue light becomes less visible when a steady blue background is turned on, but becomes more visible when yellow light is added to the blue background (Pugh and Mollon, 1979; Polden and Mollon, 1980; Pugh and Larimer, 1980). The S cone responses we describe here suggest a possible explanation: the blue background hyperpolarizes S cones, reducing the slope conductance of the calcium current and thus reducing synaptic gain. The addition of yellow light activates horizontal cell feedback, increasing the calcium current and its slope conductance, and thereby restoring synaptic gain (VanLeeuwen et al., 2009).

Our results indicate that opponent spectral interactions are created at two synaptic levels in the retina. At the S cone synapse, horizontal cell feedback creates both blue-yellow chromatic opponency and center-surround spatial opponency. Surprisingly, these large opponent surrounds are not retained by S-cone-driven ganglion cells where the yellow-OFF and blue-ON fields are more comparable in spatial extent (DeMonasterio, 1978; Solomon, et al., 2005; Field et al., 2007; Crook et al., 2009). Why is the spatial opponency of S cones no longer evident in ganglion cells? Both the S-ON and the (L+M)-OFF bipolar cells that comprise the input to the S-cone-driven ganglion cell have themselves opponent (L+M) surrounds, these surrounds reflecting horizontal cell feedback to cones. When signals from the ON- and OFF-bipolar cells are combined by the ganglion cell, the surrounds would be expected to cancel, thereby creating pure chromatic opponency with little spatial opponency (Crook et al., 2009). These receptive field properties are retained by the downstream neurons of the lateral geniculate nucleus (Wiesel and Hubel, 1966; Derrington et al., 1984). Thus it appears that the main function of yellow opponency in S cones is not to create a chromatic signal, because that function is recapitulated by circuitry in the inner retina, but rather to estimate and offset average background intensity and chromaticity so as to optimize transmission of short wavelength signals across the S cone synapse. The use of presynaptic inhibition to enhance synaptic gain may be a common modulatory mechanism utilized by the nervous system to counteract synaptic saturation and to optimize synaptic transmission. For example, activation of presynaptic GABAB receptors reduces synaptic depression in auditory nerve fibers (Brenowitz et al., 1998).

Acknowledgments

This work was supported by the Paul Kayser International Award from the Retina Research Foundation (D.M.D.), the Research to Prevent Blindness Senior Investigator Award (J.L.S.), a grant from That Man May See Inc. (J.L.S.), National Institutes of Health Grants EY06678 (D.M.D.), EY07642, (J.L.S.), RR00166 (the National Primate Research Center at the University of Washington), and Vision Research Core grants EY01730 (D.M.D.) and EY02162 (J.L.S.). We thank Dr. Edward N. Pugh for insightful discussions, Julian Vrieslander for programming assistance, and Toni Haun and Beth Peterson for technical assistance.

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