Time-resolved wide-angle X-ray scattering data from solubilized samples of bacteriorhodopsin. (a
) Integration in rings and subtraction of ‘laser off’ images from ‘laser on’ images yields the difference scattering curves as a function of the time delay (Δt
) between photoactivation and the X-ray probe. (b
) Basis spectra of an intermediate and late conformational state extracted from spectral decomposition of the data shown in (a
) Structural interpretation of these data using a rigid-body minimization routine illustrated in terms of the bacteriorhodopsin photocycle (Andersson et al.
). Reproduced from Structure
(Andersson et al.
), copyright (2009), with permission from Elsevier.
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