This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
Time-resolved wide-angle X-ray scattering data from solubilized samples of bacteriorhodopsin. (a) Integration in rings and subtraction of ‘laser off’ images from ‘laser on’ images yields the difference scattering curves as a function of the time delay (Δt) between photoactivation and the X-ray probe. (b) Basis spectra of an intermediate and late conformational state extracted from spectral decomposition of the data shown in (a). (c) Structural interpretation of these data using a rigid-body minimization routine illustrated in terms of the bacteriorhodopsin photocycle (Andersson et al., 2009). Reproduced from Structure (Andersson et al., 2009), copyright (2009), with permission from Elsevier.