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[Cover image of issue]

Cover photograph (Copyright © 2010, American Society for Microbiology. All Rights Reserved.): (Right) CTCF and PARP-1 colocalization: in situ proximity ligation assay (ISPLA). The ISPLA was carried out in MCF7 cells using both CTCF and PARP-1 antibodies. The red signal observed, as shown in the ISPLA image (center), indicates that both CTCF and PARP-1 are in close proximity to each other, either with a 40-nm maximum separation or overlapping. The blue DAPI (4′,6-diamidino-2-phenylindole) staining (top) and merge (bottom) images illustrate the extent of CTCF and PARP-1 nuclear colocalization. (Left) CTCF and PARP-1 colocalization: immunofluorescence staining. MCF7 cells were fixed and analyzed by dual immunofluorescence staining using both CTCF (green) and PARP-1 (red) antibodies. Nuclei are visualized by DAPI (blue); the merge of the green and red colors is shown in the bottom image. (See related article on p. 1199.)

Mol Cell Biol. 2010 March; 30(5): Cover