Forced expression of Runx2 leads to activation of metastatic markers
(A) Expression of MMP9, MMP13, MMP2, VEGF, OP and OC in PC3 sublines by qRT-PCR. GAPDH was used for internal control for qRT-PCR. Data shown are mean ± SD of samples analyzed in triplicates (B) Expression levels of metastasis related genes in PC3-M, PC3-L and LNCaP cells after viral transduction are shown and were normalized to GAPDH. Runx2 regulation of MMP2, MMP9, MMP13, OC and OP is shown. Data from one representative of n=4 experiments is shown. Values are mean ± SD.