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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Am J Psychiatry. Author manuscript; available in PMC 2010 February 10.
Published in final edited form as:
Am J Psychiatry. 2001 August; 158(8): 1248.
PMCID: PMC2819102

Longitudinal MRI Study of Hippocampal Volume in Trauma Survivors With PTSD



The authors prospectively explored whether a reduction in the volume of the hippocampus occurs in recent trauma survivors who develop posttraumatic stress disorder (PTSD).


Thirty-seven survivors of traumatic events were assessed within a week of the traumatic event and 6 months later. The assessment included magnetic resonance imaging of the brain (including 124 coronal slices of 1.5-mm thickness), psychometric testing, and structured clinical interviews. The Clinician-Administered PTSD Scale conferred PTSD diagnoses at 6 months.


Ten subjects (27%) had PTSD at 6 months. The subjects with PTSD did not differ from those without PTSD in hippocampal volume (right or left) at 1 week or 6 months. There was no reduction in hippocampal volume in the PTSD subjects between 1 week and 6 months.


Smaller hippocampal volume is not a necessary risk factor for developing PTSD and does not occur within 6 months of expressing the disorder. This brain abnormality might occur in individuals with chronic or complicated PTSD.

Four magnetic resonance imaging (MRI) studies have shown that chronic posttraumatic stress disorder (PTSD) is associated with lower than normal hippocampal volume (14). In the first two studies, the reported differences in hippocampal volumes between PTSD and comparison subjects ranged from 5% for the left hippocampus alone (4) to over 22% bilaterally (2); the two other studies showed an 8% smaller right hippocampus (1) and 12% smaller left hippocampus (3).

The occurrence of lower hippocampal volume in human subjects with PTSD is buttressed by animal studies (e.g., references 5, 6) that show measurable hippocampal atrophy after prolonged exposure to stress. Accordingly, distressed survivors of traumatic events may progressively develop hippocampal atrophy. However, a recent study (7) failed to show low hippocampal volume in chronically maltreated children with PTSD. Furthermore, in a study of alcoholic women (8), the presence of PTSD did not add to the effect of alcohol consumption on hippocampal volume.

The MRI studies in which smaller hippocampi were found used a cross-sectional design and evaluated individuals with chronic PTSD. Consequently, they could not determine the origin of this brain abnormality. Low hippocampal volume may either precede the traumatic event, thereby increasing the likelihood of developing PTSD (a “vulnerability” hypothesis), or develop, along with PTSD, in the aftermath of traumatic events (an “acquisition” hypothesis). Smaller hippocampal volume may also be a risk factor for developing a particularly chronic and unremitting form of PTSD.

Prospective studies, starting before the traumatic event, can determine the origin of the smaller hippocampal volume in PTSD, but studying subjects before a traumatic event is daunting. A reliable approximation of subjects' neuroanatomy before the traumatic event can nevertheless be obtained by performing MRI scans of the brain within few days of the traumatic event (the relevant animal literature will be discussed). Such a longitudinal design also reduces the influence of potentially confounding variables by using each subject as his or her own control. We present the results of a longitudinal study of hippocampal volume in PTSD in which images were obtained 1 week and 6 months after a traumatic event.



Forty-four adult survivors of traumatic events who met the DSM-IV PTSD criteria A.1 and A.2 were recruited into the study after their admission to a general hospital emergency room. Subjects were not included in the study if during the traumatic event they suffered head injury or physical injury necessitating hospital admission or major surgery. In addition, subjects with a lifetime history of neurological disorder, psychotic disorder, PTSD, or substance abuse were not included. Subjects were excluded from the study if during the study's 6-month follow-up period they experienced another traumatic event or used illicit drugs or excessive (i.e., daily) amounts of alcohol. The subjects received a comprehensive description of the study and provided written informed consent.


During the first assessment session the subjects completed the Spielberger State-Trait Anxiety Inventory, state version (9), Horowitz's Impact of Event Scale—Revised (10), and the Structured Clinical Interview for DSM-IV (11). The last instrument served to identify current and lifetime disorders. After the interview, each subject had an MRI scan of the brain. A second evaluation took place 6 months later and included the previous psychometric measures, the Mississippi Scale for Combat-Related PTSD (12, 13), and the Clinician-Administered PTSD Scale (14). The Clinician-Administered PTSD Scale conferred PTSD diagnoses and severity at 6 months. Lifetime exposure to traumatic events was assessed by the Trauma History Questionnaire (15).

MRI Acquisition and Measurement

MR images were acquired on a 2-T Elscint GYREX “Prestige” MRI system. The intracranial cavity was measured by using TE= 30 and 80 msec, TR=3000 msec, field of view=24 cm, acquisition matrix=56×256, and 192 phase-encoding steps, resulting in two double-echo images at 54 different levels with 3-mm slice thickness (contiguous slices). We used 124 coronal slices of 1.5-mm thickness (TE=5 msec, TR=35 msec, 45° angle, field of view=24 cm, acquisition matrix=256×256, 192 phase-encoding steps) to evaluate the volume of the hippocampus.

An independent rater, trained and supervised by one of us (M.E.S.) at the Department of Radiology of Brigham and Women's Hospital, performed volumetric analyses according to a previously published procedure (2, 16). This rater was blind to the subjects' diagnostic status and to the time of MR image acquisition (1 week or 6 months). Two other trained raters independently performed volumetric analyses on 12 randomly selected cases. Inter-rater reliability was evaluated by intraclass correlation, which yielded R=0.89 for the hippocampus and R=0.88 for the amygdala.


Within 48 hours of subjects' admission to the general hospital emergency room, the research psychologist (D.B.) contacted each subject and confirmed 1) the occurrence of an event involving direct threat of death or injury to self or others (DSM-IV PTSD criterion A. 1.) and 2) the presence of an intense response involving fear, helplessness, or horror (DSM-IV PTSD criterion A.2). Individuals who met these criteria and whose ages were between 18 and 65 years were invited to participate in the study. Thirty-nine (89%) of 44 consenting subjects completed the study, and 37 (95%) of those had valid MRI scans at both time points.


Ten subjects (27%) (seven women and three men) met the PTSD diagnostic criteria at 6 months, and 27 subjects (12 women and 15 men) did not. The groups did not differ significantly in gender distribution (Fisher's exact test, p= 0.26). The PTSD and non-PTSD subjects had similar mean ages—33.7 (SD=8.9) and 29.8 (SD=10.1) years, respectively—and similar mean education levels—13.4 (SD=2.0) and 12.4 (SD=3.8) years in school, respectively.

Table 1 presents the mean volumes of the hippocampus, amygdala, and total cranial cavity at 1 week and 6 months, along with concurrent psychometric test scores and total scores on the Clinician-Administered PTSD Scale. At 1 week the study groups had similar scores on the State-Trait Anxiety Inventory, state version (t=1.52, df=35, p<0.15), and Impact of Event Scale (t=1.41, df=35, p<0.13). At 6 months the PTSD group had significantly higher scores on the State-Trait Anxiety Inventory, state version (t=3.07, df=35, p<0.005), Impact of Event Scale (t=4.14, df= 35, p<0.001), Mississippi Scale for Combat-Related PTSD (t=5.94, df=35, p<0.001), and total Clinician-Administered PTSD Scale (t=6.89, df=35, p<0.0001). There was no statistically significant correlation between hippocampal volume (left or right, 1 week and 6 months) and concurrent PTSD symptoms.

Psychometric Ratings and Volumes of the Hippocampus and Amygdala at 1 Week and 6 Months in Survivors of Traumatic Events Who Did or Did Not Have PTSD at 6 Months

Three-way analysis of variance (ANOVA) using diagnosis (PTSD versus non-PTSD) as a grouping factor, time (1 week and 6 months) and side (left versus right) as within-subject factors, and volume of the hippocampus as the dependent variable revealed a nonsignificant main effect of diagnosis (F=1.26, df=1, 35, p=0.27), nonsignificant within-subject effects of time and side, and no significant diagnosis-by-time, diagnosis-by-side, time-by-side, or diagnosis-by-side-by-time interactions (F<1 in all cases). Three-way ANOVAs using the volume of the amygdala as the dependent variable revealed no significant main effect of diagnosis (F=2.08, df= 1, 35, p<0.16), a significant effect of side (F=11.58, df=1, 35, p<0.02), a nonsignificant effect of time (F<1), and no significant interactions (F<1 except for the diagnosis-by-side-by-time interaction: F=3.78, F=1, 35, p<0.06). Similar results were obtained when relative volumes (absolute volume divided by total cranial cavity) were used and when female subjects were analyzed separately.


The results of this study did not show an initial difference in hippocampal volume between trauma survivors who developed PTSD and those who did not. Smaller hippocampal volume, therefore, was not a necessary risk factor for developing PTSD in this group of subjects. In addition, individuals who developed PTSD did not show a progressive reduction of the hippocampus between 1 week and 6 months.

These results are in apparent contrast to those of the aforementioned MRI studies of PTSD (14). This discrepancy can be explained in several ways. First, structural damage to the hippocampus may result from exposure to prolonged traumatization, such as in child abuse or a 1-year war experience in Vietnam. Second, it is possible that more than 6 months of expressing PTSD are required to produce discernible reduction of the hippocampus. Third, the previous results could have been due to substance abuse or alcohol consumption, not properly accounted for by retrospective questionnaires. Finally, a smaller hippocampus may confer a specific vulnerability to highly chronic PTSD. Prolonged PTSD in Vietnam veterans has, in fact, been associated with other related findings, such as lower education level and lower premilitary IQ (see, for instance, reference 17). The current study's groups did not differ significantly in level of education.

Another explanation may be linked to the age at which the traumatic event occurred. Subcortical gray matter and limbic structures (septal area, hippocampus, and amygdala) show an increase in volume until the third decade of life (18). In two of the four previous MRI studies (3, 4) the subjects were traumatized as children. The two others (1, 2) concerned Vietnam veterans, most of whom would have served in Vietnam between the ages of 18 and 21. All four studies, therefore, examined adults whose traumatic events occurred during a period of normal development of subcortical structures. The subjects in the current study, in contrast, were older, and none was younger than 20 at the time of the traumatic event. The negative results in the study of children with PTSD by De Bellis et al. (7) might also be explained by the fact that the MRI scans of those subjects were taken when the subjects were very young, that is, in the midst of hippocampal maturation. This could have masked the full effect of the psychological insult, which may not manifest itself before adulthood.

The size of the current study group, the severity of PTSD at 6 months, and the fact that the first MRI images were acquired 1 week after the traumatic event call for a discussion of potential methodological confounds. First, a type II error might have occurred such that the current results do not exclude a true reduction of hippocampal volume in PTSD. The smallest significant reduction previously reported was 5% (4). Given the current study's finding of larger hippocampi in PTSD (3% at 1 week), the probability of having missed a hypothetical reduction of 5% in hippocampal volume between 1 week and 6 months is, for the left hippocampus, less than 4% (one-tailed t=1.77, df=35) and, for the right hippocampus, less than 3% (one-tailed t=2.01, df=35).

Second, the current study group may not have had a severe form of PTSD at 6 months. Indeed, scores of patients with chronic PTSD on the Clinician-Administered PTSD Scale are usually higher. However, the mean total score of the current PTSD group (57.90) resembles scores reported for 218 trauma survivors 4 months after the traumatic events (total score=54.6) (19) and for PTSD patients 6 months after road traffic accidents (total score=60.1) (20). Moreover, a similar mean score (58.0) was associated with a smaller hippocampus in the previously mentioned study of survivors of child abuse (4). The mean score on the Mississippi Scale for Combat-Related PTSD of the current PTSD group (110.80) is similar to the scores obtained in most studies of PTSD.

Third, a reduction of hippocampal volume could have occurred between the traumatic event and the 1-week assessment, such that the initial MRI scan may not represent pretrauma anatomy. Acute stress has, in fact, a rapid (21, 22) and reversible (23, 24) effect on hippocampal cells. The data supporting this effect, however, are mainly histological and come from studies of nonprimates. However, Fuchs and Flugge (25) have shown that measurable reduction in hippocampal volume does not occur before 21 days of continuous exposure to stress. Furthermore, in this study, a hypothetical acute stress-induced reduction in hippocampal volume should have equally affected the PTSD and non-PTSD subjects (as both groups reported substantial and similar distress at 1 week). Hence, the likelihood of having missed an acute and early reduction of the hippocampus among PTSD patients in this study is minimal.

Finally, the results of this study are in line with those from published neuropsychological studies (e.g., references 26, 27), in which hippocampal functions were not impaired in PTSD following recent traumatic events, whereas frontal lobe tasks, such as attention and set shifting, were impaired. Accordingly, the hippocampus may not be the primary dysfunctional area of the brain in PTSD following recent trauma. Moreover, reduced hippocampal volume has also been found in other prolonged mental disorders, such as depression (26), alcohol abuse (8, 28), and schizophrenia (e.g., reference 29), and hippocampal reduction in primates has been observed after prolonged social stress (6). Hippocampal atrophy, therefore, may be a common outcome of several pathogenic processes that share the element of protracted distress.


Supported by grant MH-50379 from NIMH.


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