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J Bacteriol. 2010 February; 192(4): 1160–1164.
Published online 2009 December 4. doi:  10.1128/JB.01374-09
PMCID: PMC2812974

Noncoding RNA of Glutamine Synthetase I Modulates Antibiotic Production in Streptomyces coelicolor A3(2)[down-pointing small open triangle]

Abstract

Overexpression of antisense chromosomal cis-encoded noncoding RNAss (ncRNAs) in glutamine synthetase I resulted in a decrease in growth, protein synthesis, and antibiotic production in Streptomyces coelicolor. In addition, we predicted 3,597 cis-encoded ncRNAs and validated 13 of them experimentally, including several ncRNAs that are differentially expressed in bacterial hormone-defective mutants.

In many diverse organisms, noncoding RNA (ncRNAs) have been identified as major regulators of translation efficiency and message stability (9, 23). Recent screens for ncRNAs in human predict approximately 30,000 structured RNAs (22), and many have been characterized as having a variety of regulatory functions and thus are not simply transcriptional-by-products or “transcriptional noise” (2, 5). However, the number of ncRNAs identified in bacteria is lagging behind those found in eukaryotes. Since the first publication of a fully sequenced bacterial genome, a number of genomewide computational screens for ncRNAs in microorganisms have been conducted, predicting 50 to 2,000 ncRNAs (11). ncRNAs are grouped into cis-encoded or trans-encoded classifications, depending on whether the ncRNA structure is located in the transcribed sequence of its target or found at a distance, for instance, in an intergenic region. The former type of ncRNA is reported mainly in bacterial plasmids, whereas the latter class accounts for most of the ncRNAs reported in bacterial chromosomes. These trans-encoded ncRNAs require helper proteins such as Hfq to bind to the target mRNA. They can either degrade or stabilize the target mRNA, and they are supposed to be involved in the regulation of metabolic pathways due to environmental change or stress responses (23). Recently, cis- and trans-encoded ncRNAs have been identified in mycobacterium, and the function of a trans-encoded ncRNA has been demonstrated (1). Several groups or researchers (12, 18, 20) have predicted and partially validated the existence of ncRNAs in Streptomyces, a genus of Gram-positive soil bacteria with high biotechnological relevance due to their diverse secondary metabolite production, including the production of a large variety of antibiotics of commercial importance. However, in all of these cases where trans-encoded ncRNAs were identified, their function (if any) or mode of action remained completely unknown.

In the model streptomycete Streptomyces coelicolor, no functional analysis of ncRNA has been conducted, nor are there any homologues to the main components of the RNA silencing system in the eukaryote, or the main ncRNA processing enzymes in prokaryotes, apart from homologues to enzymes, which are able to degrade double-stranded RNA. To explore a potential role of ncRNA in S. coelicolor, we first focused on the glnA gene (SCO2198), which encodes for glutamine synthase I (GSI) (6), a key regulatory target of central nitrogen metabolism and major player in the link of nitrogen assimilation to antibiotic production. An intragenic ncRNA in glnA called “cnc2198.1” was predicted (Fig. (Fig.1).1). The cnc2189.1 ncRNA was detected in early exponential phase for the wild-type and two signaling molecule-impaired strains tested by reverse transcription-PCR (RT-PCR) and tagged-primer-specific quantitative RT-PCR (qRT-PCR; data not shown) (14). Primer-specific cDNA synthesis was performed with tagged primer to retrotranscribe the antisense transcript and then used as a template for qRT-PCR analysis (see Table S2 and other supplementary material). We went on to test whether this ncRNA would also be functional, despite the absence of any previously characterized RNA-binding proteins involved in the processing of ncRNA identified in eukaryotes or prokaryotes. An antisense 88-bp fragment that overlaps with cnc2198.1 (cnc2198.1as; 120 bp) was cloned into the multicopy plasmid pIJ8781 under the control of the thiostrepton-inducible promoter tipAp (see the supplemental material). The resulting plasmid pTE313, as well as the vector pIJ8781, was introduced into S. coelicolor M145. The two strains were grown to an optical density at 450 nm (OD450) of 0.7 in SMM (19), and the expression of cnc2189.1as was induced with thiostrepton in dimethyl sulfoxide (DMSO; 5 μg/ml). Pure DMSO was used as a negative control. When cnc2198.1as expression was induced, GSI signal detected by Western analysis with a GSI antibody (dilution, 3:10,000) using 100 μg of total protein extract, decreased to 72% ± 9% and 63% ± 6% at 1 and 5 h, respectively, in comparison to M145 harboring the vector alone (Fig. (Fig.2).2). (The values are averages of three independent growth curves.) This shows that cnc2198.1as indeed affects protein expression levels of its target gene glnA by perhaps interacting with the few nucleotides of glnA mRNA (Fig. (Fig.3)3) inducing the unfolding and the complementary annealing of the two transcripts that would lead to a stall of translation and/or degradation, possibly through a mechanism independent of the classical ncRNA processing enzymes.

FIG. 1.
Location of ncRNA loci in SCO2198. SCO2198 (genomic coordinates 2364905 to 2366314) is represented in gray, while the predicted 121-bp ncRNA locus cnc2198.1 (genomic coordinates 2364929 to 2365049) and 161-bp ncRNA locus cnc2198.2 (genomic coordinates ...
FIG. 2.
Western blot analysis of GSI. Protein levels were analyzed by Western hybridization with anti-GSI specific antibodies at 1 and 5 h after induction of cnc2198.1as (M145/pTE313) expression (+) or addition of DMSO (−) in M145/pIJ8781 or M145/pTE313. ...
FIG. 3.
Predicted secondary structure of glnA mRNA and cnc2198.1as RNA. The web-based RNAfold software was used to predict the secondary structure of the glnA and cnc2198.1as transcripts. The single-stranded regions in the structures are indicated as the most ...

This molecular effect also had important and wide-ranging regulatory consequences at the phenotypic level. Not only did growth decrease (Fig. (Fig.4),4), but there was also a substantial effect on antibiotic production, the major regulated physiological process of Streptomyces. The red pigmented antibiotic undecylprodigiosin production of M145/pTE313 decreased 4-fold after induction of cnc2198.1as expression compared to the negative control and ~3.5-fold compared to the level seen in M145/pIJ8781 when measured as reported (19) (Fig. (Fig.55).

FIG. 4.
Growth curve of M145/pIJ8781 and M145/pTE313. M145/pIJ8781 and M145/pTE313 growth in liquid SMM supplemented with apramycin (1 μg/ml) was determined. Samples induced for cnc2198.1as expression by thiostrepton (+) are depicted as black ...
FIG. 5.
Antibiotic production by M145/pIJ8781 and M145/pTE313. (A) Cell pellets of M145/pIJ8781 or M145/pTE313 treated with thiostrepton (+) or DMSO (−) were collected at the time of induction (0 h) and after 3, 4.5, or 6 h. (B) The same cells ...

To explore the general relevance of ncRNAs in Streptomyces, we also conducted a computational genomewide prediction of ncRNAs by using RNAz v1.01 on the 54% of the genome of S. coelicolor that aligned with S. avermitilis as reported previously in yeast (17; see also the supplemental material). The presence of ncRNA was predicted where the RNA classification confidence value was >0.5. Of the 3,895 ncRNA elements predicted, 3,597 overlapped with 2,965 annotated coding sequences, and the remaining 298 were located in the intergenic regions. The predicted ncRNAs included all tRNAs and rRNAs annotated in RFAM and TIGR (see Table S1 in the supplemental material). Our predictions also overlapped to a large extent with those found in previous studies: 28 out 32 predictions by Pánek et al. (12) and 55 of 114 predictions by Swiercz et al. (18) were confirmed.

Total RNA was extracted as reported previously (19), and ncRNA transcripts were analyzed by primer-specific qRT-PCR (see Table S2 and other supplemental material). For each transcript, the signal was normalized to the specific transcriptional background. Signals above the negative control cnc2966 (no ncRNA predicted) were considered validated. Of the 18 predicted cis-encoded ncRNA elements that were analyzed, 13 were shown to have antisense ncRNA present either in the wild type or in two signaling molecule (γ-butyrolactone) mutants (Table (Table1).1). These 18 ncRNAs correspond to those genes which were differentially expressed in a γ-butyrolactone mutant (E. Takano et al., unpublished data). The signaling molecules are bacterial hormones that regulate antibiotic production in Streptomyces. Interestingly, two ncRNAs (cnc3669.2 and cnc3893.2) were detected only in the two signaling molecule impaired mutants and were not present in the wild type. This, for the first time, suggests that these signaling molecules are involved in the regulation of ncRNA expression in S. coelicolor.

TABLE 1.
Confirmation of predicted antisense ncRNAs

This is the first report to elucidate a cis-encoded antisense ncRNA in S. coelicolor and to show that it has a regulatory effect on cellular physiology: the overexpression of the ncRNA of the GSI gene yielded surprising growth and antibiotic production defects. This suggests that the abundant ncRNAs we identified may also play a crucial physiological role in Streptomyces. A decrease in growth by overexpression of artificial GSI antisense ncRNAs has been observed in Mycobacterium tuberculosis (8). The decrease in glutamine synthase activity led to the reduced poly-l-glutamate/glutamine in the cell wall, which in turn affects mycobacterial growth. Poly-l-glutamate has been recently identified from Streptomyces cell wall teichoic acids (16), and this may also be affected by GS activity, explaining our observed decrease in growth. Although the effect of decreased growth and attenuated antibiotic production obviously are not biotechnologically useful traits, we believe that our data demonstrate the general possibility of gene knockdown of essential and or relevant target genes by antisense ncRNA in bacteria that was thus far restricted to eukaryotes.

cis-Encoded ncRNAs in bacteria have been identified previously in a few cases (3). These were mostly restricted to plasmids, and only a handful were observed in the bacterial chromosome. For such cis-encoded ncRNAs, RNase III degrades the double-stranded RNA complex formed by binding to the target sequence (3). Although the genome of S. coelicolor does not contain obvious homologues of Hfq, which is thought to be required for the processing of trans-encoded ncRNA, it does contain an RNase III (AbsB, SCO5572) homologue. AbsB was first identified by its effect in antibiotic production (13) and was later shown to degrade double-stranded RNA (4). Recently, the deletion of the RNase III resulted in impaired antibiotic production and altered morphological differentiation (7, 15). Another possible mechanism for the effect of the overexpressed antisense ncRNA could be that the ncRNA inhibits transcription or translation (23). S. coelicolor (and probably related bacteria as well) may use these specific machinery of cis-encoded ncRNA as a novel and general regulatory mechanism, far beyond the limited plasmid-encoded examples reported earlier.

ncRNAs in eukaryotes are far more common than in bacteria and may reflect the complex regulation needed in multicellular organisms (24). The apparent abundance of functional ncRNAs we found in S. coelicolor could reflect its increased regulatory needs, given its unusually large genome (similar to yeast), an ability to adapt to a wide variety of environments and nutritional sources, and a highly dynamic secondary metabolism, including the finely tuned sequence of antibiotic production pathways. These complex regulatory networks may be mediated via signaling molecules influencing ncRNA levels. Lenz et al. (10) have shown that the expression of the trans-encoded sRNAs Qrr1 to Qrr5 in Vibrio cholerae is mediated by Hfq and dependent on LuxO (the regulator of the lux operon), whose activity is controlled by the quorum-sensing autoinducers CAI-1, Hal-1, and AI-2. Qrr1 to Qrr5 in turn repress the expression of LuxR, the main transcriptional regulator of the quorum-sensing regulon (21). ncRNA therefore seems to play a much larger role in this group of bacteria than previously suspected.

Supplementary Material

[Supplemental material]

Acknowledgments

We are grateful to Martha Merrow for the use of the ABI Prism 7500FAST system and to Wolfgang Wohlleben for the GSI and GSII antibodies. We thank Wolfgang Wohlleben and Rainer Breitling for critical reading of the manuscript.

D.D. was funded by a University of Groningen GBB bursary, and E.T. was funded by a University of Groningen Rosalind Franklin Fellowship.

Footnotes

[down-pointing small open triangle]Published ahead of print on 4 December 2009.

Supplemental material for this article may be found at http://jb.asm.org/.

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