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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Neurosci. Author manuscript; available in PMC 2010 July 29.
Published in final edited form as:
PMCID: PMC2811167
NIHMSID: NIHMS168915

Cyclin D2 is Critical for Intermediate Progenitor Cell Proliferation in the Embryonic Cortex

Abstract

Expression of cyclins D1(cD1) and D2 (cD2) in VZ and SVZ respectively suggests a switch to cD2 could be a requisite step in the generation of cortical intermediate progenitor cells (IPCs). However, direct evidence is lacking. Here, cD1 or cD2 was seen to co-label subsets of Pax6 expressing radial glial cells (RGCs), while only cD2 co-labeled with Tbr2. Loss of IPCs in cD2−/− embryonic cortex and analysis of expression patterns in mutant embryos lacking cD2 or Tbr2 indicate that cD2 is used as progenitors transition from RGCs to IPCs and is important for the expansion of the IPC pool. This was further supported by the laminar thinning, microcephaly and selective reduction in the cortical SVZ population in the cD2−/− cortex. Cell cycle dynamics between E14-E16 in knockout lines showed preserved parameters in cD1 mutants that induced cD2 expression, but absence of cD2 was not compensated by cD1. Loss of cD2 was associated with reduced proliferation and enhanced cell cycle exit in embryonic cortical progenitors, indicating a crucial role of cD2 for the support of cortical IPC divisions. In addition, knockout of cD2, but not cD1, affected both G1-phase and also S-phase duration, implicating the importance of these phases for division cycles that expand the progenitor pool. That cD2 was the predominant D-cyclin expressed in the human SVZ at 19–20 weeks gestation indicated the evolutionary importance of cD2 in larger mammals for whom expansive intermediate progenitor divisions are thought to enable generation of larger, convoluted, cerebral cortices.

INTRODUCTION

The cerebral cortex arises through a precisely timed and orchestrated series of cell divisions to generate an astonishing array of neuronal and glial cell types, ordered in a highly stereotyped laminar organization (Takahashi et al., 1995b; Caviness et al., 2003; Tarui et al., 2005). Not only does progenitor proliferation set the size of the cerebral cortex, but it also determines cortical morphogenesis, whether brain contours will be smooth or convoluted and the cerebral ventricles large or small (Chenn and Walsh, 2003; Kriegstein et al., 2006). Cortical divisions are subject to external factors and intrinsically programmed transcriptional subroutines that impact proliferation behavior of neural progenitors in a determination-stage specific manner (Noctor et al., 2004; Englund et al., 2005; Hevner et al., 2006; Shen et al., 2006; Noctor et al., 2008).

The cortical germinal epithelium was once considered relatively homogeneous, with neurogenesis occurring in the pseudostratified ventricular zone (VZ) and gliogenesis in the later arising subventricular zone (SVZ) or secondary proliferative population (Levison and Goldman, 1993; Takahashi et al., 1995a). It has since been established that neurons may arise from either the VZ or SVZ, resulting from asymmetric neurogenic divisions (primarily in the VZ) in which a neuronal daughter and a replenished progenitor result, or from symmetric neurogenic divisions (primarily in the SVZ) in which both daughters are neurons (Noctor et al., 2008). Thus, the prevailing outcomes of cytokinesis in the VZ (asymmetric daughter cells) and SVZ (symmetric daughter cells) suggest differences in the molecular machinery implementing these divisions.

We showed that two G1-phase active cyclins, cyclin D1 (cD1) and cyclin D2 (cD2), are differentially expressed in neural progenitor cells of the VZ (cD1) and SVZ (cD2) (Glickstein et al., 2007a; Glickstein et al., 2007b). Absence of either cyclin is associated with different histogenetic outcomes. Mice lacking cD2 are microcephalic despite normal body mass and parvalbumin expressing interneurons are disproportionately missing in cerebellum and cerebral cortex (Huard et al., 1999; Glickstein et al., 2007b). In contrast, mice lacking cD1 are runted but brain size is essentially normal, with no selective deficits in specific neuronal subsets (Chen et al., 2005; Glickstein et al., 2007b) and present study). Although cD2 expression in the SVZ cells suggested that cD2 may be important for the establishment of intermediate progenitors (Glickstein et al., 2007a), there is no direct evidence that cD2 is indeed expressed in transit amplifying progenitors or indication of its impact on cycling in either cortex or ganglionic eminence populations. Here, the relationships between cD1 or cD2 protein expression and radial glial cells (RGCs) or intermediate progenitor cells (IPCs) are explored. The data indicate that cD2 is critically important for establishing a full complement of IPCs, while cD1 expression is not found in this expansive population. cD2 deficiency alters cell cycle parameters in the VZ, primarily affecting G1 and S phases, but cD1 deficiency leaves cell cycle measures unchanged. Thus, cD2 is a major determinant of brain size and is more essential than cD1, due to the ability of cD2 to promote amplifying divisions.

MATERIALS AND METHODS

Animals

Mice were housed in climate-controlled Thoren units with 12h light-dark cycle. The cD2 gene was inactivated by disruption of exons I and II (Sicinski et al., 1996). The cD1 gene was inactivated by the deletion of exons I, II, and III (Sicinski et al., 1995). Heterozygous cD1+/− and cD2 +/− mice, maintained on a congenic C57BL/6J background, were bred to produce wildtype (WT) and homozygous null littermates for all studies. Tbr2 was conditionally inactivated in cortex starting at E9.5 by Foxg1-Cre mediated excision of Tbr2 exon 3 that was flanked by loxP (fx) sites (Sessa et al., 2008). Tbr2fx/+::Foxg1-Cre and Tbr2fx/fx mice were mated to produce Tbr2fx/fx::Foxg1-Cre conditional knockout (CKO) and Tbr2fx/fx control sibling embryos harvested at E13 and E14.5 (kindly provided by C-A. Mao and W. Kline, University of Texas, M. D. Anderson Cancer Center). All procedures involving animals were performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee.

Genotyping

Genomic DNA was extracted from tail snips using standard protocols and examined by PCR-based genotyping using primers for WT and null alleles as previously published (Sicinski et al., 1995; Sicinski et al., 1996; Sessa et al., 2008).

Human Tissues

Fetal brain tissue was obtained from the NICHD Brain and Tissue Bank for Developmental Disorders under contracts N01-HD-4-3368 and N01-HD-4-3383. Tissue was collected with proper consent and with the approval of ethics committees. There was no evidence of disease or developmental abnormality. Samples (n=2) were obtained from Caucasian males at 19 and 20 gestational weeks after post-mortem intervals of 1h and 4h, respectively, and storage for 11–12 years. Tissues were paraffinized, coronally sectioned (10μm) and immunostained as described below for mouse tissues.

Immunohistochemistry

Adult animals were anesthetized with ketamine/xylazine and perfused transcardially with 4% paraformaldehyde (PFA) in 0.1M phosphate buffer (pH 7.4). Embryo heads were drop-fixed in 4% PFA overnight at 4°C. Brains tissues were processed (Tissue Tech 2000, Miles Laboratories) for paraffin embedding in the coronal plane. Brains were sectioned at 4–10 μm (embryo - adult), mounted on adhesive-coated capillary gap slides (Fisher Scientific, Pittsburgh, PA) and immunostained on a TechMate 500 semi-automated stainer (Ventana Medical Systems, Tuscon, AZ) after antigen retrieval by steam in Reveal buffer (pH5.9; Biocare Medical, Concord, CA) for 30 min and cooling for 10 min. Sections were treated with 3% H2O2 for 10 min, blocked in Sniper (Biocare Medical) for 30 min and then incubated with primary antibody overnight at +4°C. Sections were then sequentially incubated in species/isotype-appropriate secondary antibody and peroxidase conjugated tertiary antibody (Signet Rabbit or Murine kit, Covance, Dedham MA) or Vectastain Elite Kit (Vector Laboratories, Burlingame, CA) for 30 min each, followed by reaction with 3, 3-diaminobenzidine (DAB) (Covance). When comparing between experimental groups or across genotypes, slides were processed together, thereby minimizing variability.

Primary antibodies included: rabbit anti-cD1 (α-cD1) (1:500, LabVision, RM-9104, Fremont, CA), mouse α-cD2(1:1000, LabVision, MS-221), rabbit α-Tbr2 (1:1000, Millipore Corp, Temecula, CA), rabbit α-cleaved caspase-3 (1:1000, Millipore), mouse α-Pax-6 (1:400, Iowa Hybridoma Bank, Iowa City, IA), rabbit α-Ki67 (1:1000, LabVision, SP6), mouse anti-BrdU (1:50, Amersham Biosciences, Piscataway, NJ), mouse α-p27 (1:10,000, Sigma-Aldrich, St. Louis, MO), rabbit α-pRb (1:200, Cell Signaling Technology, Danvers, MA), rabbit α-phosphohistone 3 (PH3) (1:1K, Millipore), mouse α-reelin (Rln) (1:500, Millipore), rabbit α-Tbr1 (1:2000, courtesy of Robert F. Hevner, University of Washington, Seattle); mouse α-Tuj1 (β–III tubulin, 1:2K, Covance), mouse α-NeuN (Millipore, 1:5K). A TUNEL labeling kit (S7101, Millipore) was used for in situ labeling of nuclei with DNA strand breaks. The specificity of the α-cD2 and α-cD1 antibodies used here has been established (Glickstein et al., 2007a).

For dual-labeling, standard reaction for cD1 or cD2 immunoperoxidase labeling was followed by Sniper block and incubation with primary antibodies against Tbr2 or Pax6 at +4°C overnight. After washing, sections were incubated in Alexa Fluor-conjugated secondary antibodies (1:500, Invitrogen Corp, Carlsbad, CA) for 1h, washed and cover-slipped with Vectashield (Vector Laboratories). At least 20 tissue sections from multiple embryos were examined to confirm or refute the co-expression of cDs with Pax6 or Tbr2 at E12.5, E14.5 and E16.5. Images of DAB-labeled nuclei were inverted and pseudocolored and subsequently overlaid with immunofluorescent images. Antibodies for BrdU and Tbr2, PH3 or Ki67 were combined for traditional dual-immunofluorescent labeling. Fluorescently labeled sections were counterstained with DAPI (Vector Labs) and immunoperoxidase-only labeled sections were counterstained with hematoxylin. Sections were photographed digitally at 4×, 10× and 20× magnification using a SPOT camera (Diagnostic Instruments, Sterling Heights, MI). Images were not modified in any way, except for adjustments of brightness and contrast.

BrdU pulse-labeling

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) was administered (50 mg/Kg, i.p.) to pregnant mice 1h or 24h before harvest date to assess the S-phase labeling index and the proliferative fraction, respectively, as enumerated in the Results. A cumulative BrdU labeling protocol was followed to determine the growth fraction (GF; fraction of the cell population that is proliferative), the time to reach the growth fraction (Tc-Ts), and subsequently, to estimate the length of the cell cycle (Tc) and the duration of S-phase (Ts) and G1-phase (TG1) (Takahashi et al., 1995b). Pregnant mice were injected with BrdU (50 mg/Kg, i.p.) starting at E14.5–E15 with survival times of 0.5h & 3.5h (after single injection at t=0h), 6.5h & 9.5h (after doses at t=0, 3h), 12.5h & 15.5h (after doses at t=0,3,6h) and 18h (after doses at t=0,3,6,9h). Other mice were sacrificed at 1.5, 2 and 2.5h following BrdU injection to estimate the duration of G2-M phase (TG2+M).

Quantification of Cell Cycle Dynamics and Statistics

All quantification was performed blind to genotype and using a standard field of view that consisted of 100 μm linear distance of the dorsomedial cortex at the mid-hemispheric level. Three fields of view for each case were averaged and all groups contained sets of WT and null littermates (tissues from 3 or more litters were examined for each parameter). Genotype averages were compared using a Student’s T-test (p<0.05 level of significance). As detailed below, some quantification paradigms used SVZ-located PH3-labeled nuclei to define the abventricular limit of the VZ equivalently in all genotypes. (1) Distribution of PH3-labeled nuclei. PH3-immunopositive nuclei were counted in the VZ (those at the ventricular wall) or SVZ (those labeled cells positioned abventricularly, typically >70 μm from ventricle). (2) S-phase labeling index. Tissues were labeled for BrdU and PH3 dual-immunofluorescence. The fraction of DAPI-labeled cells having BrdU labeling (1h following BrdU application) was calculated for the VZ (ventricular wall to one cell’s diameter ventral to PH3-labeled cells) or the SVZ (tissue above the VZ). (3) Proliferative fraction. Determined 24h following BrdU injection, this was the fraction of BrdU-positive cells that dual immunolabel for Ki67. Thus, the ‘in-cycle fraction’ represents the cells that remain in the cell cycle over a 24 hour period post-BrdU pulse. (4) The quiescent fraction. The reciprocal of the ‘in-cycle fraction’, the ‘cycle-exited fraction’ evaluates the cells labeled with BrdU only and not Ki67 and represents the proportion of cells leaving the cycle over a 24 hour period following BrdU injection, (5) Cell cycle duration. The average fraction of Nissl-labeled cells having BrdU labeling in the VZ (as defined above using PH3-labeling) was calculated for each group/genotype and the linear least squares algorithm for linear regression analysis (Excel, Microsoft Corp, Seattle, WA) was performed to estimate Tc-Ts and Ts for each genotype. This analysis was carried out as described in detail by others (Takahashi et al., 1993; Takahashi et al., 1995b; Lu et al., 2007). The fraction of PH3-labeled cells having BrdU labeling in the VZ in dual-labeled sections was calculated and the least squares algorithm was used to estimate TG2+M. TG1 was then deduced and estimates of Tc, Ts, TG1 and TG2+M and their standard errors (TG1 standard error estimated as described (Lu et al., 2007)) were statistically compared using a Z-test statistic (significance at p<0.05 if Z>1.96 on two-sided test). Cell cycle parameters estimated here were in line with those reported for C57BL/6J mice by others (i.e., (Hodge et al., 2004; Chakrabarti et al., 2007). (6) Proportion of Tbr2-immunopositive cells labeled with BrdU. Tissue from the cumulative BrdU labeling experiment (pulses at t=0,3 & harvest at t=6.5h) was labeled for BrdU and Tbr2 dual-immunofluorescence. The fraction of all Tbr2-labeled cells having BrdU-labeling was calculated. (7) Quantification of Tbr2 & Pax6 labeled cells. Single- and dual-labeled nuclei were counted in Tbr2 and Pax6 dual- immunofluorescently labeled tissue sections in a field of view that consisted of 50 μm linear distance of the dorsomedial cortex at the mid-hemispheric level in three sections per subject, which were then averaged for each case.

Quantitative Stereology

Two-dimensional and stereological counting methods were used to estimate neuron numbers and density in adult cD2−/−, cD1−/− and WT littermates using the optical fractionator (West et al., 1991) and Stereoinvestigator software (MicroBrightField Inc, Williston, VT). Nissl-labeled neurons in the somatosensory barrel field cortex of 40 μm sections (collected from fixed brains using a sliding microtome) were assessed by morphological criteria that characterize neuronal nuclei (Peters et al., 1991) and were counted in superficial (layers II-III) or deep (layers IV-VI) cortical layers. The barrel fields were analyzed in one hemisphere for each section in the series in which it was present (6–8 sections collected every 200 μm). The barrel fields were easily identifiable by the densely packed cell columns of granule cells in layer IV characteristic of this cortical region and as described previously (Glickstein et al., 2005). The medial and lateral limits included only those regions of somatosensory cortex having clearly visible barrels. Optical dissector frame and counting grid sizes of 30 and 250 μm2, respectively, permitted systematic random sampling of three to five neurons within an 8 μm depth focusing range for each sampling field and more than 200 total neurons for each subregion per case. Intra-sample coefficients of error (CE) (Schmitz and Hof, 2000) were always less than 0.05 and were equivalent across genotypes. All regions were sampled at 63× magnification in Koehler illumination conditions. The volume of the different laminar domains of interest in each subregion was estimated using the Cavalieri principle. The estimated total neuron numbers were expressed as the density per subregion, thereby controlling for the reduced brain volume in cD2−/− mice. cD2−/− and cD2+/+ littermates (from 3 litters) were examined in adulthood (8–10 wks) and cD1−/− and cD1+/+ littermates (from 4 litters) were examined at P21 due to premature death of cD1−/− mice, thought due to starvation (Sicinski et al., 1995). In WT mice, the average barrel field volumes were 1.58±0.05 mm3 (8–10wk) and 1.50±0.14 mm3 (3 wk) and the average estimated number of neurons was 2.73×105±1.8×104 (8–10 wk) and 2.67×105±2.6×104 (3 wk). The diameters of 20 randomly selected neurons per case were measured to compare neuronal size between genotypes and no differences in neuronal diameter between genotypes in any group were detected. Genotype averages were compared using a Student’s T-test (p<0.05).

RESULTS

Aberrant development of cyclin D2 (cD2−/−), but not cyclin D1 (cD1−/−) null neocortex

Developmental defects in mice deficient for cD2 result in microcephaly and selective parvalbumin interneuron deficits in forebrain and cerebellum (Huard et al., 1999; Glickstein et al., 2007b). In contrast, mice lacking cD1 are normocephalic and have no interneuronopathies (Chen et al., 2005; Glickstein et al., 2007b). The volume and neuronal packing density within the somatosensory barrel field cortex was stereologically analyzed in cD2−/−, cD1−/− and WT littermates. The cortex was thinner in cD2−/− mice as compared with cD2+/+ (Fig. 1A,B) and volume reductions were greater in the superficial (↓42%) than in the deeper (↓31%) cortical layers (Fig. 1E). In contrast, the volume of cD1−/− barrel field cortex was only modestly reduced with no exceptional shrinkage in superficial cortical layers (Fig. 1C,D,E). Neuronal packing density was comparable in cD2−/− and cD2+/+, therefore glutamatergic neuron numbers must be diminished to account for the loss in volume in cD2 nulls, since they make up the great majority of cortical neurons. Interneurons make up only a small fraction of the cortical cell population and interneuron loss alone would not be sufficient to account for a marked cortical volume reduction (Glickstein et al., 2007b). In contrast, neuronal packing density in cD1−/− was mildly elevated relative to cD1+/+ mice (Fig. 1F). Therefore, while cD1 loss was largely compensated for developmentally, cD2 deficiency was not. Moreover, the considerable deficit in superficial cortical layers suggested that cD2 was needed to maintain the progenitor pool and/or support later neurogenic events.

Figure 1
Cortical volume and neuronal density in the somatosensory barrel field cortex of cD2−/−, cD1−/− and wild-type mice

Selectively disturbed cell cycle regulation in cD2−/− embryonic neocortex

Cyclins D1 and D2 have distinct expression patterns in the embryonic forebrain with limited overlap (Glickstein et al., 2007a). Across the neurogenic period, cD1 cells were mainly localized to the ventricular zone (VZ), whereas cD2-labeled cells were more prevalent in the subventricular zone (SVZ) (Fig. 2A–F). In cD2−/− mice, there were fewer cD1-immunoreactive nuclei abventricularly with paler labeling of nuclei at the ventricular wall (Fig. 2B). In cD1−/− cortex, the distribution of cD2-labeled nuclei was more extensive in the VZ relative to that observed in cD1+/+ (Fig. 2E). In WT, these labeling patterns were consistent in nearly all cortical regions except rostromedial (nascent cingulate) cortex, where the SVZ is less distinct at this age and cD2 immunoreactivity was less apparent. Due to cD2’s SVZ-biased expression pattern, the remaining analyses were focused on the E14.5 dorsomedial cortex (mid-hemispheric level), where the SVZ is well-established; although other times in the neurogenic period were examined as noted.

Figure 2
The expression of cD2 is characteristic of intermediate progenitor cells in the cortex

Cortical progenitor specification toward glutamatergic neurons proceeds from Pax6 to Tbr2 expression (Hevner et al., 2006; Quinn et al., 2007). In the developing cortex, Pax6 expressing radial glia can divide asymmetrically to yield an RGC and an early SVZ progenitor, which is thought to undergo terminal symmetric division (Noctor et al., 2004; Gotz and Barde, 2005; Noctor et al., 2008). The transition to intermediate progenitor (transit amplifying) cells is characterized by dwindling expression of Pax6 and burgeoning expression of Tbr2 (Englund et al., 2005; Hodge et al., 2008; Pontious et al., 2008). There is not an abrupt switch from Pax6+ to Tbr2+ expression, but rather a gradient, with a subset of cells producing both Pax6 and Tbr2 simultaneously. This may account for why conditional inactivation of Tbr2 starting at E9.5 affects both superficial and deep cortical layers, though outer layers are more severely affected (Sessa et al., 2008). Nuclei expressing cD2, but not cD1, also expressed Tbr2 (Fig. 2G–L), indicating that cD2 was selectively used in IPCs. Both cD2 and cD1 immunolabeling overlapped with Pax6 immunofluorescence (Fig. 2M–R), which allowed for both cDs to have a role in radial glia. Alternatively, cD2 and Pax6 dual-labeled nuclei might represent cells that were about to or had just become intermediate progenitors, which themselves comprise a minority of cells in the VZ (Hevner et al., 2006; Noctor et al., 2008). Whether nuclei in transition (i.e., those that co-expressed Pax6 and Tbr2) selectively utilized cD2 was explored, but conclusions regarding triple labeling could not be drawn due to the transient nature of cyclin expression (Sherr, 1994; Sakaue-Sawano et al., 2008) and confidence in the detection of slight immunoreactivity. Notably, no cD1 and Tbr2 co-labeled nuclei were observed in cD2−/− mice (Supplementary Fig. 1), which suggested that cD1 could not compensate for cD2 in intermediate progenitor cell cycling.

Although their population was reduced, cD2 was not an absolute requirement for the emergence of glutamatergic neurons, which were present in the cortices of cD2 mutants. However, in E14.5 cortical neuroepithelium lacking cD2, numbers of Pax6+, Tbr2+ and Pax6/Tbr2 dual-labeled cells were significantly reduced compared to wildtype (Fig. 3A,B,E, Supplementary Fig. 2). In contrast, embryonic cortex lacking cD1 showed no reduction and indeed tended toward increased numbers of Pax6+, Tbr2+ or Pax6/Tbr2 labeled cells compared to normal siblings (Fig. 3C,D,E). Despite the differences in cell numbers in the cD2−/− embryos, the proportions of Pax6+, Tbr2+ and Pax6/Tbr2 dual labeled cells were essentially the same in wildtype, cD2−/− and cD1−/− brains (Fig. 3F) and compared well with the distribution previously observed in wildtype mice (Englund et al., 2005). Therefore, loss of cD2 reduces Pax6+, Pax6+/Tbr2+ dual labeled, and Tbr2+ progenitors proportionately, while cortical progenitor numbers are unchanged by the loss of cD1 expression. If so, the numbers of intermediate progenitors (Tbr2+ cells that pulse-label with BrdU) should also be diminished in cD2 nulls. This latter hypothesis was confirmed by significant reductions (↓38%) in the fraction of Tbr2 cells that dual labeled with BrdU 6.5h following the application of BrdU in cD2−/− mice (Fig. 3G); this pulse duration (two pulses were administered 3.5 and 6.5h prior to harvest) allowed for labeling of transitioning cells.

Figure 3
Evidence that cD2 functions in cortical progenitors transitioning from radial glia into IPCs

The proportional loss of Pax6+ only, Tbr2+ only and Pax6+/Tbr2+ cells in cD2 null embryos raises the question as to where cD2 is induced during the temporal progression of cortical progenitor development. Gene expression arrays validated by real-time PCR studies have previously shown that in the absence of Pax6, E15 mouse cortex displays a two-fold induction of cD2 compared to controls (Holm et al., 2007). Thus, Pax6 is not required to induce cD2 expression in cortical progenitors. We therefore tested whether Tbr2 expression is required for cD2 expression to appear in embryonic cortex (Fig. 3H–K). Conditional inactivation of Tbr2 in Tbr2fx/fx::Foxg1-Cre (Tbr2-CKO) embryonic cortex was compared with littermate Tbr2fx/fx (Tbr2+/+) controls at E13 and E14.5. Immunostaining for cD2 was equivalent to control at E13, but appeared to label fewer cells in the E14.5 cortex. This cD2 pattern in the Tbr2CKO parallels an enhanced cell cycle exit documented in the conditional Tbr2 mutant (Arnold et al., 2008; Sessa et al., 2008) and suggests an earlier peak in the transitioning population. Diminished cD2 expression in the E14.5 Tbr2CKO could result if Tbr2 promoted cD2 expression or simply because, as previously shown, by this age there were fewer basal progenitor cells to express cD2 (Arnold et al., 2008; Sessa et al., 2008). Thus, while it could not distinguish whether Tbr2 has a negative or positive modulatory effect on cD2, the data clearly showed that Tbr2 expression was not required for cD2 expression in cortical progenitors. Together, the data in figures 2 and and33 positioned the timing of cD2 expression in cortical progenitors as they transition from Pax6+ RGCs into IPCs (Fig. 3L).

To understand the impact of cD2 deficiency upon the cell cycle, three G1-S regulatory proteins, the cyclin-dependent kinase inhibitors p27Kip1 and p57Kip2, and phosphorylated (ser807/811) retinoblastoma (pRb) were examined in the cortex of cD2−/− and cD1−/− and WT littermates (Fig. 4). No p57-immunoreactive nuclei were observed in the cortical plate (data not shown) suggesting the importance of p27 in the cortical wall during neurogenesis. Compared with cD2+/+ embryos, p27 immunoreactivity was elevated in cD2−/− cortex across the neurogenic period (Fig. 4A,B,E,F,I,J), whereas p27 was reduced in the SVZ/VZ of cD1−/− embryos as compared with cD1+/+ or cD2+/+ tissues (Fig. 4M,N,Q,R). pRb immunolabeling was reduced in cD2−/−, most notably in the SVZ (Fig. 4C,D,G,H,K,L) and unchanged in cD1−/− (Fig. 4O,P,S,T) from E12.5 through E16.5. The increased expression of p27 and decreased expression of pRb in cD2−/− cerebral wall were consistent with slower progression through G1 phase in the absence of cD2. Overexpression or knockdown of Rb and p27 has selective effects on cell cycle duration in G1-S phase and neuron production (Sherr, 2000; Geng et al., 2001; Mitsuhashi et al., 2001; Ferguson et al., 2002). The immunolabeling results presented here indicated that cD1 and cD2 are poised to differentially regulate cell cycle progression and exert distinct effects on cortical development.

Figure 4
Cdk-inhibitor p27 and cD-Cdk effector retinoblastoma (pRb) in the cortex of cD2−/− and cD1−/− mice and WT littermates

The expression of cell cycle markers was compared in mutant and WT cortex. In cD2−/− mice, numbers of PH3-positive, M-phase nuclei were unchanged at the ventricular surface at E12.5, E14.5 and E16.5 but were substantially reduced (as much as −58%) abventricularly at E12.5 and in the SVZ at E14.5 (Fig. 5A,B,E,F,I,J & 6A). In contrast, PH3 labeled cell numbers were unaffected in cD1−/− (Fig. 5M,N & 6A). Ki67 labeling (S-G2-M-phase cells) was also reduced abventricularly in cD2−/− at E12.5, E14.5 and E16.5 (Fig. 5C,D,G,H,K,L), but was mildly increased in cD1−/− (Fig. 5O,P). Thus, the proliferating progenitor pool was diminished in the cD2−/− cortex, notably in the SVZ. Despite this reduction in the cD2−/− progenitor pool, no qualitative changes with respect to wild type siblings were observed at these embryonic ages in the distribution of postmitotic cells expressing Tbr1, a transcription factor expressed in glutamatergic neurons (Supplementary Fig. 3) or reelin, (Supplementary Fig. 4), expressed in Cajal-Retzius neurons. Thus, the output of postmitotic neurons from the neuroepithelium was likely increased in the early-mid neurogenic period. TUNEL and activated caspase-3 immunolabeling detected no differences in apoptosis among cD2−/−, cD1−/− and WT cortices during the neurogenic period (Supplementary Fig. 5), or postnatally (Glickstein et al., 2007b).

Figure 5
Proliferation markers phosphohistone 3 (PH3) and Ki67 are altered in the cortex of cD2−/− relative to cD1−/− and WT littermates
Figure 6
Reduced proliferation and early progenitor cell cycle exit is observed in cD2−/− cortex at E14.5

Cell cycle dynamics were further explored (Fig. 6, Tables 1, ,2,2, Supplementary Tables 1&2). At E14.5, the number of PH3-labeled, M-phase cells in the VZ did not significantly differ in cD2−/− and cD1−/− compared with wildtype siblings (Fig. 6A). However, compared to WT and cD1−/− mice, the number of abventricular M-phase cells was sharply decreased in the cD2 nulls, showing almost 60% fewer PH3+ cells in the SVZ, but no significant decrease in VZ mitoses (Fig. 6A). In addition, fewer BrdU pulse-labeled, S-phase nuclei (1h after BrdU administration) were present in both the VZ and the SVZ in cD2 with respect to either wildtype controls or cD1 nulls (Fig. 6B, Supplementary Table 1). Compared to wildtype, the S-phase labeling index was reduced more severely in the cD2−/− SVZ than VZ (43% vs. 29%) (Fig. 6B). Therefore, although cell cycle dynamics were affected in both VZ and SVZ compartments of cD2 nulls, the SVZ was more severely impacted by the loss of cD2 expression.

Table 1
Duration of cell cycle phases in the VZ of cD2−/−, cD1−/− and WT littermates (cD2+/+, cD1+/+).
Table 2
Summary of G1-modulator expression and cell cycle analyses in cD2−/− and cD1−/− cortex as compared with WT littermates.

To explore whether there was an enhanced rate of terminal divisions to account for depletion of the progenitor pool in the cD2−/− cortex, we measured the fraction of BrdU+ progenitors expressing Ki67 and therefore remaining in cycle 24h following BrdU administration. The in-cycle fraction was reduced in cD2−/− but was unchanged in cD1−/− as compared with WT at all times examined, (reductions in P: E16.5[dbl greater-than sign]E12.5, E14.5) (Fig. 6C & Supplementary Table 2). Conversely, the cycle-exited fraction was increased, indicating that more progenitor cells exited the cell cycle in cD2−/− than in WT or cD1−/−, consistent with premature terminal differentiation of progenitors in the absence of cD2, but not cD1. The expression of neuroblast and neuron markers Tuj1 and NeuN, which encroach upon the VZ in cD2−/− as compared with WT and cD1−/−, further supported this conclusion (Supplementary Fig. 6).

In order to further examine neocortical cell cycle dynamics in the absence of D2 or D1 cyclins, cell cycle phase-durations were analyzed in cD2 and cD1 null mutants (Table 1). Measuring the duration of individual phases of the cell cycle is not possible in the SVZ (Takahashi et al., 1995a). However, in the VZ the length of the cell cycle phases was estimated between E14.5 and E15.5 in cD2−/−, cD1−/− and WT using a cumulative BrdU pulse-labeling paradigm (Takahashi et al., 1995b). There were no significant differences in the growth fraction (GF) between genotypes, which supported that delayed migration in any genotype did not unduly influence results. The duration of each phase did not significantly differ in cD1−/− as compared with cD1+/+ or cD2+/+. In contrast, the duration of G1 (TG1) was substantially prolonged and, the duration of S phase (Ts) was significantly reduced in cD2−/− tissues (Table 1). As a result, the duration of the cell cycle (Tc) was mildly, yet non-significantly increased in cD2−/− VZ. The prolonged G1-phase duration (Table 1) was consistent with the observation of enhanced cell cycle exit in Fig. 6C and Supplementary Table 2, since lengthening of G1 has been associated with progenitor exit from the cell cycle (Calegari and Huttner, 2003; Tarui et al., 2005).

cD2 is the predominant cyclin D in the human SVZ

The SVZ is thought to serve a fundamental role in the massive expansion of the cerebral hemispheres, the development of upper cortical layers and potentially, in gyrification in the second trimester of human fetal gestation (Kriegstein et al., 2006). We explored whether cD2 was also the predominant D-type cyclin in the human SVZ at 19 gestational weeks. At this age, the VZ is marginal with respect to the substantially expanded SVZ, which is bisected into an inner and outer SVZ by incoming fibers (Zecevic et al., 2005) (Fig. 7B,C). cD1 showed scant labeling throughout cerebrum and the few nuclei observed were always in the inner SVZ (Fig. 7D). In contrast, immunolabeling for cD2 was robust in both the VZ and the SVZ, in both medial and lateral SVZ and was observed in both the outer and the inner SVZ (inner SVZ [dbl greater-than sign] outer SVZ) (Fig. 7E). The same areas showing robust cD2 immunoreactivity were co-labeled for Tbr2 (Fig. 7F). cD2 may thus serve an important evolutionary role in brain development, as it plays a vital role in the cycling of Tbr2-immunopositive cells that can modulate human brain size (Baala et al., 2007).

Figure 7
cD2 is the predominant cyclin D in the human SVZ

DISCUSSION

Previous histological data show that cD1 and cD2 predominate in distinct neuroepithelial populations and that cD2 localizes to SVZ where transit-amplifying divisions occur (Glickstein et al., 2007a; Glickstein et al., 2007b). The present study provides a number of further insights into D-cyclin regulation of cortical histogenesis. First, it was formerly possible that cD2 is expressed only in IPCs. The present paper shows that cD2 is expressed as cells transition from RGCs to IPCs. Second, cD1 is not expressed in IPCs. Third, it was formerly possible that no IPC could arise without cD2. The present study shows that cD2 is not an absolute requirement for Tbr2+/BrdU+ IPCs to appear.

Fourth, cD2 is important for the expansion of IPC numbers. The location of cD2 labeling, dual expression with Tbr2, and cD2-deficit effects on the size of the precursor pool subsets including Pax6+/Tbr2+ and Tbr2+/BrdU+ cells indicate that cD2 is the D-type cyclin used in these expansive IPC divisions. This assertion is further supported by the degree of microcephaly, greater thinning of superficial cortical layers and significant reduction in the SVZ population in the cD2−/− compared to cD1 nulls (Figs 3, ,6).6). The additional loss of Pax6+ progenitors in cD2 nulls, the continued expression of cD2 in Pax6−/− cortex (Holm et al., 2007) and in Tbr2−/− cortex (Fig. 3) further localizes cD2 function to cells transitioning between RGCs and IPCs, and is consistent with the effect of cD2 loss on both progenitor pools. It is also consistent with the more pronounced impact of cD2 loss on IPCs, in which abventricular mitoses are significantly diminished in cD2 null embryonic cortex at E14.5, while ventricular mitoses, the majority of which occur in cell cycles that use cD1, are no different from wildtype siblings. The effect of cD2 loss on IPCs is no less dramatic than that of Insm1 (insulinoma-associated-1) protein that was recently reported to have a role in expansion of basal progenitors in developing neocortex (Farkas et al., 2008), yet Insm1 inactivation only reduces the Tbr2+ IPC population (Tbr2+/PH3 dual labeled cells across both basal and apical locations) by approximately 50% (Farkas et al., 2008). This compares well with our data that IPCs still arise in the absence of cD2 but with a nearly 40% decrease in Tbr2+/BrdU+ dual labeled cells. Together, data presented here indicate that cD2 is not necessary in order for IPCs to emerge, but that cD2 is used in progenitors as they transition into and expand their IPC numbers.

Fifth, the present manuscript shows that cD2 regulates not only the length of G1 phase but also influences the length of S-phase (Ts) which has been postulated to be important in the regulation of primate cortex lamination. Sixth, the Tbr2CKO expresses cD2 even in the absence of Tbr2, suggesting that cD2 emerges temporally before Tbr2. However, since a smaller population of Tbr2+ cells still arises in cD2−/− cortex, there must be cD2-independent routes to Tbr2 expression, and cD2 is important in cortex primarily to promote the expansion of the IPC pool. Finally, cD2 expression predominates in the human SVZ at 19–20 weeks gestation, supporting a significant role for this protein in the expansion of brain size over the course of species evolution.

Cell cycle parameters estimated here were in line with those reported for C57BL/6J mice (Hodge et al., 2004; Chakrabarti et al., 2007), but longer than reported in CD1 mice (Takahashi et al., 1995b). Both estimates of TG2+M and Tc were calculated using independent groups within each genotype and were increased to a similar extent (~20%) in both wildtype strains compared with CD1 mice at the same age (Takahashi et al., 1995b). Strain dependent factors may account for the longer estimates of cell cycle times in C57BL/6J mice observed here and by others. Regardless of differences in wildtype values compared with the literature, cD2+/+ and nulls showed fundamental differences in cell cycle parameters determined in a well-controlled study.

The cumulative BrdU labeling experiments were instructive. Despite the relatively small proportion of cD2-expressing cells in the apical progenitor pool, loss of cD2 expression had a measurable impact on cell cycle dynamics in the VZ. Cell cycle length (Tc) tended to be prolonged in the cD2 nulls but not in cD1−/− mice. G1-phase (TG1) was prolonged a striking 4 hours in embryos without cD2, confirming lack of cD1 induction in the absence of cD2. Conversely, the unchanged TG1 or Ts in cD1 nulls supported the effective compensation of cD1 loss by cD2 induction. TG1 lengthening was consistent with an unopposed increase in expression of the cdk inhibitor p27, just as acute induction of p27 prolonged TG1 in transgenic mice (Mitsuhashi et al., 2001). However, subsequent direct measurement of cell cycle phases in p27 overexpressors and knockouts, has led to the consensus that modulation of p27 levels increases the rate of cell cycle exit during neurogenesis without significantly changing Tc, TG1, or Ts (Tarui et al., 2005). Thus, the observed effect of cD2 loss on G1 may result from combinatorial changes in p27, phosphorylated Rb and/or other cell cycle components yet to be identified. One caveat is that previous data investigating p27 overexpression was gathered between E12–E14, while the present experiments in cD2 nulls examined the interval between E14.5–E16, when p27 effects on cell cycle parameters may be more robust. The increased fraction of cells leaving the cycle in cD2 nulls was consistent with the measured lengthening of TG1, since pharmacological inhibition of G1-active cdks prolongs G1, pushing cortical progenitors out of cycle (Calegari and Huttner, 2003; Calegari et al., 2005).

A striking insight from the BrdU cumulative labeling was the contraction of S-phase (Ts) in cD2 nulls. This was unexpected, since G1-active cD2 is not known to have effects on S-phase. Loss of cD2 resulted in a ‘catch up’ so that Tc in the cD2 nulls was close to wildtype. One possible mechanism for shortening of Ts could be that a cyclin E-cdk2 buildup needed to overcome the higher set-point created by elevated p27 (unopposed by D-cyclin) served to propel the cell through S-phase, once the G1-S checkpoint was traversed. Although immunohistochemical staining for cyclin E was attempted in the cD2 nulls, localization with the available anti-cyclin E antibodies did not permit a conclusive determination. Similarly, existing phospho-cdk2 antibodies cross react with other cdks (e.g. cdk5). Thus tests of this hypothesis await more specific/sensitive reagents. Nevertheless, the determination that absence of cD2 significantly lengthens TG1 and shortens Ts in neural progenitors is unique in the examination of cell cycle mouse mutants and suggests that events in both G1 and S phases may be important to the influence exerted by cD2. Indeed, changes in Ts across the period of cortical neurogenesis have been observed in primates, where biphasic modulation of Ts has been related to cortical expansion and “laminar elaboration” (Kornack and Rakic, 1998; Dehay and Kennedy, 2007). That cD2 is the predominant D-cyclin expressed in the human SVZ at 19–20 weeks gestation indicates the evolutionary importance of cD2 in larger mammals for whom expansive intermediate progenitor divisions have been proposed to enable the generation of larger, convoluted, cerebral cortices (Noctor et al., 2008; Pontious et al., 2008). It may be that molecular programs associated with cD2 allow for changes in Tc and Ts sufficient to be detected in cD2 deficient mouse cortex and that are essential in primate corticogenesis.

These data fit a model in which cyclin D2 is required for expansive cortical cell divisions (Fig. 7G–I). In Pax6 expressing apical progenitors, a relatively small number of cells using cD2 undergo symmetric stem divisions to enlarge the VZ progenitor pool (Fig 7G). Asymmetric divisions driven by cD1 maintain the population by generating a daughter that will leave the VZ to become a neuron, glial, or intermediate progenitor cell and another daughter that replenishes the radial glial pool (Fig 7H,I). Cells becoming Tbr2 expressing intermediate progenitors use cD2 for that critical symmetric transit amplifying division (Fig 7H). Without either cD1 or cD2, progenitors exit the cell cycle and differentiate (Fig 7H, I), evident in the increased cell cycle exit in cD2−− cortex. The preponderance of cD2 expression in human embryonic SVZ strongly supports this hypothesized requirement of cD2 to promote expansive transit amplifying divisions. The fact that double knockout of cD2 and cD1 is not compensated by cD3 expression in brain (Ciemerych et al., 2002) and that cortical neuroepithelial cells still proliferate in the absence of all D-cyclins (Kozar et al., 2004) suggest that cD3 does not play a significant role in either cD2 or cD1 null embryonic cortex. The disparity in cD2 expression between the VZ and SVZ is consistent with the volume differences between the ventricles and convoluted cortex, and cell number differences from the deep to superficial cortical layers needed to construct a ‘gyral’ brain (Kriegstein et al., 2006; Noctor et al., 2008). Thus, cD2 may be a key evolutionary determinant of cortical size and organization among species.

Supplementary Material

Supplementary Data

Acknowledgments

We are indebted to Dr. Chai-An Mao and Dr. William Klein, University of Texas, M. D. Anderson Cancer Center for generously providing fixed embryo brains from Tbr2fx/+::Foxg1-Cre x Tbr2fx/fx intercrosses used in this study. Supported by NIH grant # PO1NS048120 to MER.

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