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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Nature. Author manuscript; available in PMC 2010 February 27.
Published in final edited form as:
PMCID: PMC2809436
NIHMSID: NIHMS162582

EBV induced molecule-2 mediates B cell segregation between outer and center follicle

Abstract

B cell follicles are specialized microenvironments that support events necessary for humoral immunity 1, 2, 3. Following antigen encounter, activated B cells initially seek T cell help at the follicle-T zone boundary and then move to interfollicular and T-zone distal (outer) regions of the follicle 4, 5, 6, 7, 8, 9, 10. Subsequently, some cells move to the follicle center, become germinal center (GC) B cells and undergo antibody affinity maturation 1, 2, 11. Although germinal ‘centers’ within follicles were described in 1885 12, the molecular cues mediating segregation of B cells between outer and center follicle have remained undefined. Here we establish a role for the orphan G-protein coupled receptor, Epstein Barr Virus-induced molecule-2 (EBI2) 13, in this process. EBI2 is expressed in mature B cells and increases in expression early after activation before being down-regulated in GC B cells. EBI2 deficiency led to a reduction in the early antibody response to a T-dependent antigen. EBI2-deficient B cells failed to move to the outer follicle at day 2 of activation and instead were found in the follicle center, whereas EBI2 over-expression was sufficient to promote B cell localization to the outer follicle. In mixed bone marrow chimeras, EBI2-deficient B cells phenocopied GC B cells in preferentially localizing to the follicle center. When down-regulation of EBI2 in wild-type B cells was antagonized, participation in the GC reaction was impaired. These studies identify an important role for EBI2 in promoting B cell localization in the outer follicle, and show that differential expression of this receptor helps position B cells appropriately for mounting T-dependent antibody responses.

The propensity of B cells to migrate to outer versus center follicle at different stages of the antibody response, together with the established roles of G-protein coupled receptors (GPCRs) in controlling lymphocyte positioning events, led us to ask whether novel GPCRs differentially expressed between early activated and GC B cells may be involved in this subcompartmentalization. These criteria focused our attention on EBI2 (GPR183), a Gαi-coupled orphan receptor 13,14 abundantly expressed in EBV infected and activated human B cells and down-regulated in GC B cells 15,16. To explore the expression pattern of mouse Ebi2 we generated a reporter mouse line carrying the EGFP gene inserted in place of the Ebi2 open reading frame (Suppl. Fig. 1). Analysis of Ebi2GFP/+ mice showed that EBI2 is upregulated during B cell maturation in the bone marrow (BM), and is expressed in mature recirculating B cells in BM, spleen and lymph nodes (Fig. 1a). Expression of the GFP reporter tracked closely with changes in Ebi2 transcript abundance (Fig. 1b). GFP levels were further upregulated after BCR engagement with anti-IgM, or combined anti-IgM and anti-CD40 stimulation (Fig. 1c). To examine expression following receipt of T cell help we employed an adoptive transfer system in which B cells from C57Bl/6 (B6) Ebi2GFP/+ mice were transferred to the coisogenic strain, bm12, that bears a three amino acid difference in the I-Ab MHC class II molecule and contains a high frequency of I-Ab responsive helper T cells 6,17. This approach permits tracking of an activated B cell population in non-transgenic mice at early time points after receiving T cell help. Two days after adoptive transfer of anti-IgM stimulated B cells we found higher GFP expression in B cells transferred to bm12 compared to B6 recipients, indicating that interaction with helper T cells promoted EBI2 upregulation (Fig. 1d). EBI2 expression was maintained in plasma cells but was markedly (~25-fold) down-regulated in GC B cells (Fig. 1a and b). Most CD4 T cells and a smaller fraction of CD8 T cells also expressed EBI2, though at lower levels than B cells (Fig. 1a and b). In sections, GFP was detectable throughout the follicle and T zone but was almost undetectable within GCs, identified by their expression of GL7, making these structures appear as EBI2-deficient islands in a ‘sea’ of EBI2-expressing cells (Fig. 1e).

Figure 1
Ebi2 upregulation in activated B cells and down-regulation in GCs

An initial analysis of lymphoid tissues from EBI2-deficient mice showed the presence of organized follicles and T cell compartments, and the mice had normal numbers of B and T cells (Suppl. Fig. 2 and data not shown). Movement of activated B cells to the follicle-T zone boundary within six hours of BCR stimulation occurred similarly for EBI2-deficient and wild-type cells (Fig. 2a) suggesting that BCR mediated EBI2 induction (Fig. 1) is not required for this CCR7-dependent relocalization event 8. We then asked whether the next stage(s) of activated B cell migration that occur during T-dependent responses, movement to the outer follicle and interfollicular regions, were EBI2-dependent. To permit in situ tracking of activated B cells responding to T cell help, we used the bm12 adoptive transfer approach introduced above. Littermate control B cells that had received anti-IgM stimulation were able to respond to T cell help within bm12 recipients and relocalized to the outer follicle at day 2 (Fig. 2b), as did cotransferred wild-type Igha B cells (Fig. 2b), consistent with earlier studies using Ig-transgenic B cells 6. By contrast, EBI2-deficient B cells were unable to localize to this region and instead favored the central area of the follicle (Fig. 2b). To determine whether upregulation of EBI2 could be sufficient to control B cell localization to the interfollicular and outer follicle regions within lymphoid tissues we transduced B cells with Ebi2-encoding or control retroviruses and transferred them to wild-type recipients. One day later the Ebi2-overexpressing cells, identified by expression of a hCD4 reporter, were situated in interfollicular regions and in the outer follicle (Fig. 2c). This contrasted with the distribution of B cells transduced with the control retrovirus, where the cells distributed uniformly within the follicle (Fig. 2c). Thus EBI2 appears to be both necessary and sufficient to promote positioning of activated B cells in the outer follicle and interfollicular regions. Consistent with an essential role for EBI2 in the correct positioning of B cells during the early phases of T-dependent humoral responses, EBI2-deficient mice mounted a reduced day 7 IgG1 antibody response to nitrophenyl-chicken gamma globulin (NP-CGG) in alum (Fig. 2d). The IgM response was not affected (Fig. 2d).

Figure 2
EBI2 promotes localization of activated B cells in the outer follicle

As another approach to examine the role of EBI2 in determining B cell localization, we examined cell distribution in 20:80 mixed BM chimeras that contained a minority of EBI2-deficient cells (20% Ighb EBI2-deficient or littermate control and 80% Igha wild-type). Remarkably, EBI2-deficient B cells selectively localized in a GC-like location at the center of follicles in spleen, lymph nodes and Peyer's patches of the BM chimeras (Fig. 3a). These foci of cells were not GCs as they maintained high expression of IgD and lacked expression of GL7 (Fig. 3a and Supp. Fig. 3). In contrast, EBI2-deficient and wild-type T cells appeared intermingled throughout the T zone (Suppl. Fig. 4). Analysis of 90:10 mixed BM chimeras containing a majority of EBI2-deficient (or littermate control) cells showed that EBI2-deficient B cells colocalized with the CD35+ follicular dendritic cell (FDC) network at the center of follicles whereas the minor population of wild-type B cells in these mice was partially excluded from this area and enriched in interfollicular regions or outer follicles (Fig. 3b). Similar findings were made in lymph nodes and Peyer's patches (Suppl. Fig. 5). It seems possible that in mice where most B cells lack EBI2, there is increased EBI2 ligand availability and wild-type B cells predominate at sites of ligand production. In control mixed BM chimeras reconstituted with a minority (20:80) or majority (90:10) of Ebi2+/+ BM, the two types of wild-type B cells were intermingled in both the follicle center and periphery (Fig. 3a, b and Suppl. Figs. 3 and 5). FDCs are dependent on LTα1β2 for their maintenance 18. To test whether the segregation of wild-type and EBI2-deficient B cells was dependent on FDCs, we treated mixed BM chimeras with LTβR-Ig, a LTα1β2 antagonist 19, for 2-3 weeks. The CD35+ FDC networks were depleted after treatment (Fig. 3c) as expected 19,20. Under these conditions, wild-type and EBI2-deficient cells no longer showed a segregated distribution in splenic B cell areas (Fig. 3c) suggesting a role for FDCs or other LTα1β2-dependent cells in this process. In mice that lack CXCL13, a chemokine made broadly by the follicular stromal cell network 20,21, B cell localization in the outer splenic white-pulp (where follicles would normally be located) is reduced but not completely blocked 22 (Fig. 3d). In CXCL13-deficient mice reconstituted with a mixture of EBI2-deficient and wild-type BM, EBI2-deficient B cells were selectively diminished within the white-pulp cords and instead accumulated in the marginal zone that surrounds the white-pulp (Fig. 3d). These findings provide further evidence for EBI2-mediated attraction of B cells to the outer white-pulp.

Figure 3
EBI2-deficient B cells localize to the follicle center in a LTα1β2- and CXCL13-dependent manner

The above findings suggested that EBI2 down-regulation in GC B cells may promote their positioning at the follicle center. To directly test the significance of EBI2 down-regulation during GC development, we enforced constitutive EBI2 expression in hen egg lysozyme-specific Ig-transgenic B cells using retroviral gene transduction and then tested their ability to participate in GC and plasma cell responses following short-term adoptive transfer to hen egg lysozyme immunized hosts (Fig. 4a). The frequency of Ebi2 or control vector transduced B cells amongst the GC and plasma cell populations was tracked using the hCD4 reporter. By flow cytometric analysis, Ebi2-transduced cells showed a reduced ability to participate in GC responses compared to vector transduced cells, while participating with wild-type efficiency in the plasma cell response (Fig. 4a and b). Although some Ebi2-transduced cells could take on a GC phenotype (Fig. 4a), the cells were unable to position in GCs (Fig. 4c and Suppl. Fig. 6). Cells transduced with the control vector were readily detected within GCs (Fig. 4c and Suppl. Fig. 6). Ebi2 transduced cells contained Ebi2 transcripts in amounts within two-fold of those present in follicular B cells and at least 25-fold higher than in GC B cells (Suppl. Fig. 6). These experiments suggest that EBI2 down-regulation is necessary for localization of developing GC B cells to the follicle center.

Figure 4
Maintained Ebi2 expression impairs participation in GC response

In summary, we establish that EBI2 is upregulated in B cells after BCR and CD40 engagement and is necessary to promote positioning of activated B cells to interfollicular regions and the outer follicle. Cognate B cells interact with helper T cells and undergo proliferation in these regions 7,10. Defects in these processes are likely responsible for the diminished ability of EBI2-deficient mice to mount an early T-dependent IgG antibody response. Our findings suggest EBI2 is needed within B cells for these events but we do not exclude that it also plays a role in directing activated CD4 helper T cells and possibly other cell types to these regions. GC B cells markedly down-regulate EBI2, a change that appears necessary to favor localization of activated B cells at the follicle center, in association with the antigen-presenting and GC-supportive 23 FDC network. Bcl6, a transcription factor required for GC development, negatively regulates EBI2 expression 15. We suggest that by favoring appropriate niche occupancy, negative regulation of EBI2 represents an important component of the Bcl6 gene expression program directing GC over plasma cell fate. Although the ligand for EBI2 remains undefined, we speculate that it is more concentrated in the outer compared to center follicle as well as in interfollicular regions. We propose that while CXCR5 is sufficient to promote B cell localization in follicles 22,24, cells expressing EBI2 are more strongly attracted to the outer follicle compared to cells lacking this receptor. An additional FDC-derived cue may favor positioning in the center follicle, and when cells lose responsiveness to EBI2 ligands, positioning in response to this cue is dominant. The activity of EBI2 provides a possible explanation for why CXCR5-deficient B cells continue to localize in interfollicular areas and in regions corresponding to the outer follicle 22,24. Our findings may also help explain the niche preferences of certain B cell lymphomas, particularly follicular center lymphoma 25. Indeed, expression array studies demonstrate that EBI2 is down-regulated in follicular and GC lymphomas 16,26. Moreover, it seems possible that the marked EBI2 induction observed early following EBV infection 13 serves as a mechanism used by the virus to promote positioning in niches that favor survival of the infected B cells.

Methods Summary

EGFP was inserted in place of the Ebi2 open reading frame within E14 (129) ES cells using standard procedures and Ebi2GFP/+ mice were backcrossed to C57Bl/6 for six generations. 6-12 week old C57BL/6 were from either the National Cancer Institute or Jackson Laboratories. B6(C)-H2-Ab1bm12/KhEgJ (bm12) mice and B6.Cg-IghaThy1aGPi1a/J (IgMa) mice were from Jackson Laboratories. MD4 mice 27 and CXCL13-deficient mice 22 were from an internal colony. Bone marrow chimeras were generated as described 11 and analyzed after 6-12 weeks. NP-CGG immunizations were performed using 50 μg of NP-CGG (Solid Phase Sciences, USA) in Alum (Accurate Chemical & Scientific Corp., USA). The retroviral construct was made by inserting the mouse EBI2 open reading frame, with a preprolactin-FLAG leader sequence 28 in place of the ATG, into the MSCV2.2 retroviral vector containing cytoplasmic-domain truncated human CD4 as an expression marker downstream of the internal ribosomal entry site 8. B cells were isolated and in some cases labeled with 2.5 μM of 5(and 6)-carboxy-fluorescein diacetate succinimidyl ester (CFSE, Molecular Probes) as described 29. For in vivo analysis of Ebi2 expression, 5-10×106 purified B cells as in 11, were transferred into bm12 experiments. Transduced cells were adoptively transferred one day after spin-infection for transfers to non-immunized hosts or immediately after spin-infection for transfers to immunized hosts. For GC experiments, B6 mice received 105 MD4 B cells and 105 OTII CD4+ T cells at day -1, were intraperitoneally immunized with 50μg HEL-OVA in RIBI adjuvant system (Sigma) at day 0 and received approximately 106 EBI2 or control vector transduced cells at day 1. Mice were analyzed on day 5. Flow cytometry, ELISA, immunohistochemsitry and immunofluorescence microscopy were by standard techniques and are detailed in Full Methods.

Supplementary Material

Acknowledgments

We thank Chris Allen for help with microscopy and discussions, Jesse Green, Betsy Gray and Kazuhiro Suzuki for helpful advice and Jinping An for expert assistance with the mouse colony. We also thank Nigel Killen and Jay Dietrich for help with gene targeting. J.P. is an Associate and J.G.C. an Investigator of the Howard Hughes Medical Institute. This work was supported in part by grants from the NIH.

Footnotes

Author Contributions: J.P.P. and J.G.C. designed and conceptualized the research; J.P.P., L.K. and Y.X. did the experiments. J.P.P., L.M.K. and J.G.C. analyzed the data, prepared the figures and wrote the manuscript.

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