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J Cell Biol. 2009 December 14; 187(6): 752.
PMCID: PMC2806321
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Membranes enter transfer negotiations

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Osh2p (green) is enriched where the ER (red) is close to the plasma membrane.

A family of yeast proteins can bridge adjacent membranes and transfer sterol lipids between them, say Schulz et al. The process may allow one organelle to regulate the lipid composition of another.

To maintain the correct level of sterols that they need to function, organelles exchange the lipids through vesicular and nonvesicular pathways. In yeast, the latter mechanism requires the Osh family of sterol-binding proteins and is thought to occur at membrane contact sites, where organelles are positioned extremely close to each other. How Osh proteins facilitate lipid transfer is unknown, however.

Schulz et al. discovered that Osh proteins can bind two different organelles simultaneously, due to the existence of distinct membrane-binding domains on either side of their structure. One of these domains is next to the protein's sterol-binding pocket, and probably positions the pocket so it can easily extract a sterol molecule or deliver it to a target membrane. But the other site may regulate this process by interacting with phospholipids in another membrane close by. When the second site was mutated in Osh4p, the protein could still transfer sterols between liposomes in vitro but—unlike the wild-type protein—the rate of exchange wasn't enhanced by the presence of PI(4,5)P2. Moreover, Osh4p lacking the second membrane-binding site couldn't transfer sterol in cells.

The researchers found that most Osh proteins localize to membrane contact sites, where they likely transfer sterols between closely apposed organelles. The direction of transfer might be controlled by the phospholipids in each membrane, says senior author William Prinz, if the protein prefers to release sterol when its second membrane-binding site is contacting a particular phosphoinositide.

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