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Logo of bmcgenoBioMed Centralsearchsubmit a manuscriptregisterthis articleBMC Genomics
 
BMC Genomics. 2009; 10: 626.
Published online Dec 23, 2009. doi:  10.1186/1471-2164-10-626
PMCID: PMC2805700
Generation and analysis of expression sequence tags from haustoria of the wheat stripe rust fungus Puccinia striiformis f. sp. Tritici
Chuntao Yin,1 Xianming Chen,1,2 Xiaojie Wang,3 Qingmei Han,3 Zhensheng Kang,3 and Scot H Hulbertcorresponding author1
1Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA
2US Department of Agricultural Research Service, Wheat Genetic, Quality, Physiology and Disease Research Unit, Pullman, WA 99164-6430, USA
3College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaanxi, 712100, PR China
corresponding authorCorresponding author.
Chuntao Yin: chunto_yin/at/wsu.edu; Xianming Chen: xianming/at/wsu.edu; Xiaojie Wang: sealover2001/at/gmail.com; Qingmei Han: hanqm9/at/163.com; Zhensheng Kang: kangzs/at/nwsuaf.edu.cn; Scot H Hulbert: scot_hulbert/at/wsu.edu
Received June 17, 2009; Accepted December 23, 2009.
Abstract
Background
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most destructive diseases of wheat (Triticum aestivum L.) worldwide. In spite of its agricultural importance, the genomics and genetics of the pathogen are poorly characterized. Pst transcripts from urediniospores and germinated urediniospores have been examined previously, but little is known about genes expressed during host infection. Some genes involved in virulence in other rust fungi have been found to be specifically expressed in haustoria. Therefore, the objective of this study was to generate a cDNA library to characterize genes expressed in haustoria of Pst.
Results
A total of 5,126 EST sequences of high quality were generated from haustoria of Pst, from which 287 contigs and 847 singletons were derived. Approximately 10% and 26% of the 1,134 unique sequences were homologous to proteins with known functions and hypothetical proteins, respectively. The remaining 64% of the unique sequences had no significant similarities in GenBank. Fifteen genes were predicted to be proteins secreted from Pst haustoria. Analysis of ten genes, including six secreted protein genes, using quantitative RT-PCR revealed changes in transcript levels in different developmental and infection stages of the pathogen.
Conclusions
The haustorial cDNA library was useful in identifying genes of the stripe rust fungus expressed during the infection process. From the library, we identified 15 genes encoding putative secreted proteins and six genes induced during the infection process. These genes are candidates for further studies to determine their functions in wheat-Pst interactions.
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