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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
J Neurosci. Author manuscript; available in PMC 2010 March 30.
Published in final edited form as:
PMCID: PMC2805078
NIHMSID: NIHMS155989

Mitochondrial Ca2+ Cycling Facilitates Activation of the Transcription Factor NFAT in Sensory Neurons

Abstract

Ca2+-dependent gene regulation controls many aspects of neuronal plasticity. Significant progress has been made toward understanding the roles of voltage- and ligand-gated Ca2+ channels in triggering specific transcriptional responses. In contrast, the functional importance of Ca2+ buffers and Ca2+ transporters in neuronal gene regulation is less clear despite their critical contribution to the spatio-temporal control of Ca2+ signals. Here we examined the role of mitochondrial Ca2+ uptake and release in regulating the Ca2+-dependent transcription factor NFAT that has been implicated in synaptic plasticity, axonal growth and neuronal survival. Intense stimulation of sensory neurons by action potentials or TRPV1 agonists induced rapid activation and nuclear import of NFAT. Nuclear translocation of NFAT was associated with a characteristic prolonged [Ca2+]i elevation (plateau) that resulted from Ca2+ uptake by, and its subsequent release from mitochondria. Measurements using a mitochondrial Ca2+ indicator, mtPericam, showed that this process recruited mitochondria throughout the cell body, including the perinuclear region. [Ca2+]i levels attained during the plateau phase were similar to or higher than those required for NFAT activation (200–300 nM). The elimination of the [Ca2+]i plateau by blocking either mitochondrial Ca2+ uptake via the uniporter or Ca2+ release via the mitochondrial Na+/Ca2+ exchanger strongly reduced nuclear import of NFAT. Furthermore, preventing Ca2+ mobilization via the mitochondrial Na+/Ca2+ exchanger diminished NFAT-mediated transcription. Collectively, these data implicate activity-induced Ca2+ uptake and prolonged release from mitochondria as a novel regulatory mechanism in neuronal excitation-transcription coupling.

Keywords: Ca2+, NFAT, mitochondria, TRPV1, mtPericam, DRG neurons

INTRODUCTION

Activity-dependent gene regulation underlies many forms of neuronal plasticity, including structural and functional changes that take place during development, synaptic remodeling associated with learning and memory formation, and long-term alteration of neuronal function in neurological disorders (Deisseroth et al., 2003; Cohen and Greenberg, 2008). Ca2+ plays the central role in these processes, by coupling electrical activity to the activation of various Ca2+-dependent transcription factors. One of the fundamental questions of Ca2+-dependent gene regulation is how the spatio-temporal organization of neuronal Ca2+ signals determines the transcriptional outcome. Recent studies established the importance of the Ca2+ entry mechanisms (e.g., via NMDA receptors versus the L-type Ca2+ channels) in triggering specific transcription pathways (Deisseroth et al., 2003; Greer and Greenberg, 2008). In contrast, the role of Ca2+ pumps, exchangers and Ca2+-transporting organelles in neuronal gene regulation is poorly understood, despite the fact that these systems profoundly affect the amplitude, duration and subcellular localization of Ca2+ signals produced by neuronal activity (Miller, 1991; Thayer et al., 2002). In this study we examined how Ca2+ uptake and release by mitochondria contributes to neuronal regulation of the transcription factor NFAT (nuclear factor of activated T-cells).

NFAT is a Ca2+/calcineurin (CaN)-dependent transcription factor that has been implicated in the development and function of various organ systems, including the immune, endocrine and cardiovascular systems (Crabtree and Olson, 2002; Hogan et al., 2003). Recent studies have highlighted the importance of NFAT in neurons, where it is activated by electrical activity and neurotrophins, and is involved in the control of synaptic plasticity, axonal growth and neuronal survival (Graef et al., 1999; Graef et al., 2003; Groth and Mermelstein, 2003; Benedito et al., 2005; Oliveria et al., 2007). NFAT is exquisitely sensitive to the duration of the Ca2+ signal (Dolmetsch et al., 1997), which is explained by its activation mechanisms. In resting cells, phosphorylated NFAT resides in the cytosol. It is dephosphorylated by CaN in response to an increase in [Ca2+]i, resulting in unmasking of its nuclear localization signal (NLS) and its import into the nucleus. There, NFAT partners with other transcription factors to initiate transcription. Once [Ca2+]i returns to basal levels, GSK3β and several other protein kinases rephosphorylate NFAT, which promotes Crm-1-mediated NFAT export from the nucleus (Crabtree and Olson, 2002; Hogan et al., 2003). Thus, NFAT-mediated transcriptional responses depend on the balance between the activities of CaN and NFAT kinases. Consequently, retaining NFAT in the nucleus requires continuous CaN activity and persistent [Ca2+]i elevation.

In lymphocytes and other non-excitable cells, the prolonged [Ca2+]i elevation essential for NFAT signaling is mediated by so-called Ca2+ release-activated Ca2+ channels or CRACs (Feske et al., 2006). In neurons, however, the nature of the mechanisms that sustain NFAT activation is not fully understood. Here, by simultaneously imaging [Ca2+]i changes and NFAT dynamics within single neurons, and by monitoring expression of NFAT-reporter genes, we demonstrate for the first time that prolonged Ca2+ release from mitochondria significantly contributes to NFAT activation in neurons and facilitates the function of NFAT as a nuclear integrator of repetitive electrical activity.

MATERIALS AND METHODS

Cell Cultures and Transfection

Cultures of DRG neurons were prepared from newborn Sprague-Dawley rats, as previously described (Usachev and Thayer, 1999). In brief, newborn (1–3 day-old) Sprague-Dawley rats (Charles River Laboratories, Wilmington, MA) were sacrificed according to a protocol approved by the University of Iowa Institutional Animal Care and Use Committee. DRG were dissected from the thoracic and lumbar segments and incubated in pronase E dissolved in DMEM (1 mg/ml) for 7 min at 37°C in a 10% CO2 incubator. Ganglia were washed twice in cold DMEM containing HEPES (20 mM; pH=7.4) and then mechanically dissociated by trituration with flame-constricted Pasteur pipettes of decreasing diameter. Suspensions of DRG cells were plated onto 25 mm glass coverslips coated with poly-L-ornithine and laminin. Cells were grown in DMEM medium supplemented with 5 % heat-inactivated horse serum, 5% fetal bovine serum and penicillin-streptomycin (100 U/ml and 100 μg/ml, respectively) in a 10% CO2 incubator at 37°C and used within 2–3 days after plating. Transfection of DRG neurons was performed before plating, using an Amaxa electroporation system (Program G-13; Lonza, Switzerland), as previously described (Schnizler et al., 2008), except for the experiments shown in Figure 1D, for which the cells were transfected using gene gun (Usachev et al., 2002). The EGFP-NFAT1 (c2) plasmid was a gift of Dr. Rao (Harvard University) (Aramburu et al., 1999), the EGFP-NFAT2 (c1) plasmid was a gift of Dr. Olson (University of Texas Southwestern Medical Center) (Chin et al., 1998), the GFP-NFAT4 (c3) plasmid was a gift of Dr. Iino (The Tokyo Metropolitan Institute of Medical Science) (Tomida et al., 2003), the EGFP-NFATc4 plasmid was a gift of Dr. Graef (Stanford University) (Graef et al., 1999) and was also obtained from Addgene Inc. (plasmid 10961; Cambridge, MA) and the mtPericam plasmid was a gift of Dr. Miyawaki (RIKEN, Japan) (Nagai et al., 2001).

Figure 1
Expression and pharmacological characterization of NFAT in DRG neurons

Simultaneous Imaging of [Ca2+]i and (E)GFP-labelled NFAT isoforms

DRG neurons were transfected with one of the following plasmids, EGFP-NFATc1, EGFP-NFATc2, GFP-NFATc3 or EGFP-NFATc4, and in 24–48 hrs, cells were loaded with the Ca2+-sensitive indicator Fura-2 (Fura-2/AM). Cells were placed in a flow-through chamber mounted on the stage of an inverted IX-71 microscope (Olympus, Japan) and perfused with the standard extracellular HEPES-buffered Hank’s salt solution (HH buffer) composed of: 140 mM NaCl, 5 mM KCl, 1.3 mM CaCl2, 0.4 mM MgSO4, 0.5 mM MgCl2, 0.4 mM KH2PO4, 0.6 mM NaHPO4, 3 mM NaHCO3, 10 mM glucose, 10 mM HEPES, pH 7.4 with NaOH (310 mOsm/kg with sucrose). Fluorescence was alternately excited at 340 (12 nm bandpass), 380 (12) nm and 475 (12) nm using the Polychrome IV monochromator (TILL Photonics, Germany) and focused on the cells via a 40x oil-immersion objective (NA=1.35, Olympus). Emitted fluorescence was collected at 530 (50) nm using an IMAGO CCD camera (640×480 pixels; TILL Photonics). A 2×2 binning of the camera (each pixel corresponds to ~500 nm) was used for most of the experiments, except for those shown in Supplemental Figures 3C, 3D and 4A–C, for which the CCD camera was used at its maximal spatial resolution (no binning; each pixel corresponds to ~250 nm). Series of 340, 380 and 475 nm images were sampled every 5, 10 or 20 s. Fluorescence was corrected for background at each wavelength, as determined in an area that did not contain any cell. The fluorescence ratio (R=F340/F380) was converted to [Ca2+]i according to the formula: [Ca2+]i = Kdβ(R−Rmin)/(Rmax−R) (Grynkiewicz et al., 1985). Rmin, Rmax and β were determined by applying 10 μM ionomycin in Ca2+-free buffer (1 mM EGTA) and HH buffer (1.3 mM Ca2+) (Usachev and Thayer, 1999). Translocation of (E)GFP-labelled NFAT isoforms was analyzed by calculating mean intensities of (E)GFP fluorescence in the cytosol and nucleus and expressed as the (E)GFP-NFAT nuclear/cytosolic ratio. Data were processed using TILLvisION 4.0.1.2 (TILL Photonics) software and presented as mean ± SEM.

[Ca2+]i elevation and nuclear import of NFAT isoforms were induced by trains of action potentials, TRPV1 agonists or high K+-induced depolarization. Two different depolarization protocols were used throughout the study. For the first protocol, strong depolarization was evoked by a brief (30 or 180 s) application of 90 mM KCl (K+90). Such stimulation produced large-amplitude [Ca2+]i elevations (>1 μM), which engaged mitochondria and led to the generation of prolonged [Ca2+]i plateau and robust NFAT translocation, and essentially mimicked the [Ca2+]i and NFAT responses induced by trains of action potentials or by TRPV1 agonists. The second depolarization protocol was designed to generate sustained [Ca2+]i elevations of relatively small amplitude (<300–500 nM), which are independent of mitochondria. For this weak-depolarization protocol, 10, 15, 20 or 25 mM of KCl were applied for various time periods ranging from 10 to 60 min, which, in turn, determined the duration of [Ca2+]i elevations. Indeed, no [Ca2+]i plateau was observed for such weak depolarizations, and [Ca2+]i rapidly returned toward the baseline upon repolarization. In this protocol, the L-type Ca2+ channel activator BayK8644 (1 μM) was added to the K+10, K+15, K+20 and K+25 solutions, as we found that this drug helped to stabilize [Ca2+]i at the corresponding elevated levels.

In some experiments [Ca2+]i elevations and nuclear import of NFAT were induced by the Ca2+ ionophore ionomycin (10 μM). Extracellular Ca2+ concentration ([Ca2+]o) was set to the low submicromolar levels in these experiments by mixing 5 mM EGTA with either 4.3 or 4.4 mM CaCl2 in the extracellular solution of the following composition: 140 mM NaCl, 5 mM KCl, 1 mM MgCl2, 10 mM glucose, 10 mM HEPES, pH 7.4 with NaOH (310 mOsm/kg with sucrose). The resulting [Ca2+]o was determined by fluorescent microscopy using a 100 μl droplet of the described solutions supplied with 50 μM Fura-2. Combining 5 mM EGTA and 4.3 mM CaCl2 yielded a [Ca2+]o of 171 ± 8 nM (n=5 independent measurements); a combination of 5 mM EGTA with 4.4 mM CaCl2 yielded a [Ca2+]o of 255 ± 24 nM (n=5 independent measurements).

Measurement of Mitochondrial Ca2+ concentration [Ca2+]mt

Mitochondrial Ca2+ concentration ([Ca2+]mt) was measured using the mitochondria-targeted Ca2+ indicator mtPericam (Nagai et al., 2001), as previously described (Medvedeva et al., 2008). mtPericam fluorescence was excited at 410 (12) nm via a 60x oil-immersion objective (NA=1.40, Olympus) and collected at 530 (50) nm at the sampling rate of 0.2 Hz (Fig. 2F) or 2 and 10 Hz (Fig. 2E) using the same fluorescent microscope as described above. A 2×2 digital binning was used in these experiments, which corresponds to ~330 nm/pixel (60x objective; 640×480 pixels IMAGO CCD camera; TILL Photonics). [Ca2+]mt changes were presented as −ΔF/F0=−(F−F0)/F0, where F is current fluorescence intensity and F0 is fluorescence intensity in the resting cell. Fluorescence was corrected for background, as determined in an area that did not contain any cell.

Figure 2
Bursts of action potentials and TRPV1 agonists activate NFAT in DRG neurons

Gene Reporter Assays

DRG neurons were transfected with the NFAT-luciferase (firefly) reporter plasmid (2.5μg of cDNA/reaction; pNFAT-TA-luciferase; Clontech, Palo Alto, CA) alone or together with a plasmid encoding a Renilla reniformis luciferase controlled by a constitutive HSV-TK promoter (0.25 μg of cDNA/reaction; pRL-TK; Promega, Madison, WI). On the next day, cells were stimulated with K+10, K+15, K+20 or K+25 solutions (prepared by mixing 150 mM KCl with complete DMEM) containing 1 μM BayK8644 for various durations (see Results for details). Specifically, for the experiments described in Figure 1C, which examined NFAT activation as a function of the duration of depolarization (and [Ca2+]i elevation), the periods of depolarization using K+20 were 2, 6 and 12 hrs. For the experiments described in Figures 3E and 3F, which examined NFAT activation as a function of the magnitude of depolarization (and [Ca2+]i elevation), cells were treated with K+10, K+15, K+20 or K+25 for 12 hrs. Cells were always lysed 12 hrs after beginning of the stimulation and subsequently analyzed using the Promega single- or dual-luciferase protocol.

Figure 3
Quantification of NFAT activation as a function of [Ca2+]i in DRG neurons

An EGFP-based reporter plasmid (pNFAT-TA-EGFP) was constructed by replacing the luciferase portion of the pNFAT-TA-luciferase construct (Clontech) with the EGFP coding sequence of the pEGFP-N1 construct (Clontech). DRG cultures were transfected with pNFAT-TA-EGFP, 20 hrs later stimulated with 90 mM KCl and after an additional 12 hrs in culture, were analyzed for EGFP expression in individual DRG neurons using the same fluorescence microscopy setup as described above. EGFP-positive cells were defined by setting the discrimination threshold to 115 arbitrary units (a.u.), which is more than 3 standard deviations (SD=5.6 a.u.) greater than the mean cellular autofluorescence intensity (95.2 a.u.; n=21; determined in untransfected cultures). All measurements were performed using identical configurations and parameters: 100 ms exposure; 2×2 binning; λex=475 (12) nm; λem=530 (50) nm; 40x oil-immersion objective (NA=1.35, Olympus).

RT-PCR and Real-Time RT-PCR Analysis

Enriched neuronal DRG cultures were prepared as previously described (Usachev et al., 2002). Total RNA was isolated using the RNase easy mini kit (Qiagen, Valencia, CA) and reverse transcribed and amplified using the one-step RT-PCR kit (Qiagen) according to the manufacturer’s protocols. The following sets of primers specific for rat NFATc1, NFATc2, NFATc3 and NFATc4 were synthesized (Integrated DNA Technology, Coralville, IA) and used: NFATc1, F, 5′-CAA-CGC-CCT-GAC-CAC-CGA-TAG-3′; NFATc1, R, 5′-GGC-TGC-CTT-CCG-TCT-CAT-AGT-3′; NFATc2, F, 5′-ACA-TCC-GGG-TGC-CCG-TGA-AAG-T-3′; NFATc2, R, 5′-CTC-GGG-GCA-GTC-TGT-TGT-TGG-ATG-3′; NFATc3, F, 5-CTA-CTG-GTG-GCC-ATC-CTG-TTG-T-3; NFATc3, R, 5-AGC-TCT-TGA-GCA-GAT-CGC-TGA-GAG-CAC-TC-3; NFATc4, F, 5-AAC-ATG-GCT-GCC-AAC-ATT-GA-3; NFATc4, R, 5-CCC-GCT-TGT-TGC-TGT-ACT-CA-3. RT-PCR reactions were performed (Hybaid PCR Sprint Thermal Cycler, MA, USA) under the following conditions: 50°C for 30 min for reverse transcription, followed by95°C for 15 min for initial PCR activation, then 95°C for 1 min, 55°C (50°C for NFATc2) for 1 min, 72°C for 1 min at 30cycles, and 72°C for 10 min. Amplified transcripts were separated by electrophoresis in 1% gel agarose and detected using ethidium bromide.

To determine the absolute copy number of each NFAT isoform, we performed SYBR green based real-time RT-PCR. The SYBR green I nucleic acid gel stain (Cat. # S7563, Invitrogen, Carlsbad, CA) was added to RT-PCR reactions (one-step RT-PCR kit, Qiagen), which were carried out using the Bio-Rad CFX96 system (Bio-Rad, Hercules, CA). In order to construct standard curves, the amount of NFAT plasmid DNA (GFP-NFATc1, Addgene plasmid 11101; EGFP-NFATc2, provided by Dr. Rao; GFP-NFATc3, provided by Dr. Iino; EGFP-NFATc4, Addgene plasmid 10961) was measured using a NanoDrop-1000 spectrophotometer (Thermo Scientific, Wilmington, DE), after which each plasmid was serially diluted to copy numbers ranging from 9.4 × 102 to 1.0 × 108 per reaction. The NFAT plasmid dilutions and the NFAT transcripts in the total RNA isolated from neuron-enriched cultured rat DRG were amplified with the following isoform specific primer sets: NFATc1, F, 5′-CAT-CAA-CGC-CCT-GAC-CAC-3′, R, 5′-GTG-GTG-CCC-AGG-TCT-TCC-3′, NFATc2, F, 5′-TCT-GCT-GTT-CTC-ATG-GAT-GC-3′, R, 5′-TCA-GGA-CTG-GTC-TTC-CAT-ATC-3′, NFATc3, F, 5′-GAT-CAA-GCT-GCC-ATA-CTA-CCA-3′, R, 5′-CTG-AGA-TCC-AAG-GCC-ATC-ATC-3′, NFATc4, F, 5′-GAG-CAG-CTG-GAG-CTG-AGG-3′, R, 5′-TGT-AGC-CTA-GGA-GCT-TGA- C-3′. The program for the real-time RT-PCR was 50°C for 30 min for reverse transcription, 95°C for 15 min for PCR polymerase activation, then 94°C for 30 s, 55°C for 30 s, 72°C for 30 s at 30 cycles. The cycle threshold (Ct) value of each NFAT plasmid dilution was plotted against the logarithm of the corresponding plasmid copy number. Then the standard curves for each NFAT isoform were constructed by a linear regression with plotted points. The absolute copy number of each NFAT isoform was calculated by relating its Ct value to the corresponding standard curve. The specificity of the amplicons was determined by both a melting curve analysis and a 3 % agarose gel analysis. The PCR efficiency was 1.87 ± 0.01 for NFATc1, 1.91 ± 0.02 for NFATc2, 1.85 ± 0.02 for NFATc3 and 1.89 ± 0.01 for NFATc4, as determined by the slope of the standard curve according to the equation: Efficiency = 10(−1/slope).

Reagents

Fura-2/AM was obtained from Invitrogen (Carlsbad, CA); capsaicin, CGP37157, cyclosporin A and NADA were from Tocris (Ellisville, MO); ionomycin was from Calbiochem (San Diego, CA); FK-506 and oligomycin were from Alomone Labs (Israel); and leptomycin B was from LC Laboratories (Woburn, MA). All other reagents were purchased from Sigma (St. Louis, MO).

RESULTS

Characterization of NFAT signaling in rat DRG neurons

Using RT-PCR, we detected the transcripts for NFATc1, NFATc2, NFATc3 and NFATc4 isoforms in DRG neurons (Fig. 1A). Further analysis using quantitative real-time RT-PCR showed that NFATc3 transcript predominates among the NFAT isoforms expressed in DRG neurons (Fig. 1B and Supplemental Fig. 1). We also examined the transcriptional activity of endogenous NFAT using an NFAT-luciferase expression reporter (Clontech, CA). Depolarization (20 mM KCl) for 2, 6 and 12 hrs induced a marked increase in the expression of the reporter gene, which depended on the duration of depolarization and was completely abolished by addition of the CaN inhibitors cyclosporin A (CsA; 1 μM; Fig. 1C) or FK-506 (200 nM; Fig. 1C).

When activated, NFAT translocates to the nucleus. To further characterize NFAT regulation in DRG neurons, we transfected cells with a plasmid encoding NFATc3 (major NFAT isoform in DRG neurons) fused with a green fluorescent protein (GFP-NFATc3), as previously described (Jackson et al., 2007). Subsequently, DRG neurons were loaded with the Ca2+ indicator Fura-2, and [Ca2+]i and GFP-NFATc3 dynamics were monitored simultaneously. Nuclear translocation of NFATc3 was quantified by calculating the ratio of the mean nuclear/cytosolic GFP fluorescence (Fig. 1E–G). This approach permits repeated quantitative measurement of NFATc3 dynamics in real time, and enables one to relate NFAT localization to changes in [Ca2+]i. GFP-NFATc3 was excluded from the nuclei in the resting state (Fig. 1D). Depolarization using 90 mM KCl (K+90) induced a rapid [Ca2+]i elevation throughout the cell body, which was followed by nuclear translocation of GFP-NFATc3. Nuclear import of NFATc3 was essentially complete within ~10 min. The return of [Ca2+]i to baseline levels induced NFATc3 export from the nucleus, and the protein relocated to the cytosol within ~15 min (Fig. 1E–G and Movie 1).

The CaN inhibitor FK-506 (200 nM) completely blocked depolarization-induced NFAT activation, but had no effect on the [Ca2+]i transients (Fig. 1E; n=6). The effect of another CaN inhibitor, CsA (5 μM), was virtually identical (n=5; not shown) to that of FK-506. These data once again emphasize the key role of CaN in mediating the Ca2+-dependent activation and nuclear import of NFAT in DRG neurons. Previous studies showed that activated CaN remains associated with NFAT in the nucleus, which prevents binding of the exportin Crm-1 to NFAT, thereby promoting nuclear retention of NFAT (Zhu and McKeon, 1999). These findings also imply that sustained NFAT activation requires the spread of [Ca2+]i elevation to the nucleus. It is likely that similar mechanisms are involved in NFAT regulation in DRG neurons. Indeed, we found that the selective Crm-1 inhibitor leptomycin B (100 nM) prevented nuclear export of NFAT even after [Ca2+]i recovered to baseline levels (n=4; Supplemental Fig. 2).

A sustained activation of CaN requires superoxide dismutase (SOD) to protect CaN from inactivation by superoxide radicals (O2) (Wang et al., 1996) that are produced in mitochondria in response to strong Ca2+ entry (Bito et al., 1996; Hongpaisan et al., 2004). Consistent with this idea, we found that the inhibition of SOD by diethyldithiocarbamic acid (DETC) significantly reduced depolarization-induced nuclear import of NFATc3 in DRG neurons (n=11; Fig. 1F). A free radical scavenger, N-acetylcysteine (NAc, 5 mM), prevented the inhibitory effect of DETC on NFATc3 translocation (n=7; Fig. 1G). Interestingly, NAc did not significantly affect depolarization-induced NFATc3 dynamics when applied alone (n=9), suggesting that O2 levels were already low in DRG neurons under control conditions, most likely due to the activity of SOD.

Intense electrical stimulation and TRPV1 agonists activate NFAT in DRG neurons

Next we examined NFAT activation in response to the firing of action potentials. DRG neurons were stimulated using a single train (10 Hz for 10 s) or four trains (15 Hz for 10 s, 60 s apart) of action potentials (Fig. 2A, B and Movie 2). These protocols are consistent with the firing patterns observed in primary afferent fibers in response to thermal or mechanical stimulation in vivo (Meyer and Campbell, 1981; Slugg et al., 2000). In addition, similar stimulation protocols were used by others for studying transcription responses in sensory neurons (Fields et al., 1997; Brosenitsch and Katz, 2001; Eshete and Fields, 2001). Action potential stimulation elicited large and long- lasting elevations of [Ca2+]i in both the cytosol ([Ca2+]cyt) and the nucleus ([Ca2+]nuc), which were accompanied by a rapid nuclear translocation of NFATc3 (Figs. 2A, B). Notably, there was only a short (30–40 s) delay between the [Ca2+]i rise and the initiation of NFAT translocation. The nuclear/cytosolic ratio of GFP-NFATc3 fluorescence was 0.57 ± 0.03 (n=16) in resting cells and increased to the peak values of 1.54 ± 0.13 (n=13; range 0.82 – 2.11) and 2.53 ± 0.69 (n=3; range 1.32 – 3.72) in response to a single and four bursts of action potentials, respectively (Fig. 2A, B).

A subpopulation of nociceptive sensory neurons express the vanilloid receptor TRPV1, which serves as a molecular integrator of pain-producing stimuli, such as noxious heat, protons and lipid-derived endovanilloids (Caterina and Julius, 2001; Bhave and Gereau, 2004; Lu et al., 2006; Szallasi et al., 2007). The TRPV1 agonist capsaicin (1 μM, 30 s) induced a robust and prolonged increase of both [Ca2+]cyt and [Ca2+]nuc in 12 out of 27 medium- and small-size DRG neurons transfected with GFP-NFATc3. In each of the capsaicin-responsive neurons, the [Ca2+]i elevation was followed by nuclear import of NFATc3 (Fig. 2C). The nuclear/cytosolic GFP-NFATc3 fluorescence ratio was 0.45 ± 0.02 in resting cells and reached the level of 1.84 ± 0.15 (n=12; range 1.25 – 2.74) after the capsaicin treatment. An endogenous TRPV1 agonist, N-arachidonoyl-dopamine or NADA (Huang et al., 2002), also induced a large and long-lasting [Ca2+]i increase and the nuclear translocation of NFATc3 (n=3; Fig. 2D). Thus, action potentials and TRPV1 agonists rapidly activate NFATc3 in DRG neurons.

A common feature of the described Ca2+ responses is the secondary prolonged [Ca2+]i plateau phase that follows the initial [Ca2+]i peak (Fig. 2A–D). The [Ca2+]i plateau induced by large Ca2+ loads in peripheral and central neurons is mediated by mitochondria that buffer Ca2+ influx during stimulation via the uniporter mechanism and subsequently release accumulated Ca2+ back into the cytosol via the mitochondrial Na+/Ca2+ exchanger (Miller, 1991; Pivovarova et al., 1999; Friel, 2000; Thayer et al., 2002). During the plateau phase, [Ca2+]i remained elevated in all parts of the cell body, suggesting a wide-spread engagement of mitochondria in Ca2+ transport (Fig. 2A–D). We confirmed this using the mitochondria-targeted Ca2+ indicator mtPericam (Nagai et al., 2001) to monitor mitochondrial Ca2+ concentration ([Ca2+]mt) in response to action potentials (n=11; Fig. 2E and Movie 3) and capsaicin (n=5; Fig. 2F), respectively. Notably, Ca2+ uptake by, and Ca2+ release from, mitochondria were observed throughout the cell body, including sub-plasma membrane areas and the perinuclear region.

Does the described wide-spread and prolonged [Ca2+]i plateau contribute to NFAT activation in sensory neurons? In order to answer this question, we first performed a quantitative analysis of NFAT activation as a function of [Ca2+]i in DRG neurons.

Quantification of NFAT activation as a function of [Ca2+]i in DRG neurons

[Ca2+]i changes and GFP-NFATc3 dynamics were studied simultaneously following a stepwise depolarization by raising extracellular K+ concentration ([K+]o) from 5 to 10, 15, 20 and 25 mM, respectively (Fig. 3A). As a result, [Ca2+]i also increased in a stepwise manner. Elevation of [Ca2+]i to above 200–300 nM triggered NFATc3 import into the nucleus, whereas a decline in [Ca2+]i to below approximately 300 nM (n=5) upon repolarization initiated NFATc3 export from the nucleus (Fig. 3A).

In order to perform a more detailed examination of nuclear NFAT import as a function of [Ca2+]i, we used a modified protocol in which neurons were treated for 40–60 min with one of the following high-K+ solutions: K+10, K+15, K+20 or K+25 (Fig. 3B). The magnitude and the rate of NFAT translocation in each neuron were subsequently analyzed. The results of these experiments are summarized in Figure 3 and in Supplemental Figure 3. Specifically, we found that K+10-induced depolarization triggered nuclear translocation of GFP-NFATc3 in 4 of 6 DRG neurons tested, whereas K+15 evoked GFP-NFATc3 translocation in 7 of 8 neurons (Supplemental Fig. 3E). The corresponding [Ca2+]i peak elevations were 143 ± 30 nM (n=6) and 475 ± 84 nM (n=8) for the K+10- and K+15-induced depolarizations, respectively. Stronger stimulations, such as those induced by K+20- or K+25-containing solutions, evoked NFAT nuclear import in all DRG neurons examined (n=7 for K+20 and n=5 for K+25). Plotting the magnitude of NFATc3 translocation as a function of [Ca2+]i peak elevation for each individual neuron, revealed that [Ca2+]i increases above ~220 nM were sufficient to induce NFATc3 translocation in all the neurons tested (Supplemental Fig. 3E). Given that the introduction of an exogenous mobile high-affinity Ca2+ buffer such as Fura-2 could potentially alter the spatio-temporal characteristics of [Ca2+]i signals, we repeated analysis of NFATc3 translocation in response to K+10, K+15, K+20 and K+25-induced depolarizations in DRG neurons that had not been loaded with the indicator. Overall, we found that the magnitudes and the rates of NFATc3 nuclear translocations were similar between Fura-2-loaded neurons and those that had not been loaded with the indicator (Fig. 3C, D).

In smooth muscles, sustained highly localized [Ca2+]i elevations (persistent Ca2+ sparklets) near the sites of Ca2+ entry through the L-type Ca2+ channels were shown to activate NFATc3 (Nieves-Cintron et al., 2008). Although we have not detected Ca2+ sparklets in response to depolarization (Supplemental Figs. 3 and 4), the possibility that such local events took place in DRG neurons cannot be completely ruled out. If microdomains of highly elevated [Ca2+]i near the plasma membrane are important for NFAT activation in DRG neurons, then the described high-K+ depolarization experiments might have overestimated the [Ca2+]i sensitivity of NFAT. Given these considerations, we further inspected [Ca2+]i dependence of NFAT activation by using two additional alternative approaches to elevate [Ca2+]i. For the first approach, we examined the Ca2+ dependence of GFP-NFATc3 nuclear translocation by inducing Ca2+ leak from the endoplasmic reticulum (ER) Ca2+ stores with a specific SERCA (sarco-endoplasmic reticulum Ca2+-ATPase) inhibitor, cyclopiazonic acid (CPA; 5 μM) (Usachev and Thayer, 1999; Lu et al., 2006; Usachev et al., 2006). CPA-induced Ca2+ leak from the ER Ca2+ stores is a slow process (compared to Ca2+ release mediated by ryanodine or inositol triphosphate receptors) (Camello et al., 2002) and is unlikely to be associated with the generation of [Ca2+]i “hot spots”, which requires both high-conductance Ca2+ channels and a steep Ca2+ gradient. Indeed, CPA induced a gradual and homogenous [Ca2+]i elevation throughout the cell (Supplemental Fig. 5A; see also (Usachev et al., 2006)). We found that 5 μM CPA induced nuclear translocation of GFP-NFATc3 in 12 of 21 DRG neurons tested (Supplemental Fig. 5). For the responding neurons in which CPA triggered nuclear import of NFAT, the peak [Ca2+]i elevation was 130 ± 10 nM (n=12; range: 100 – 210 nM); for non-responding neurons, the peak of the CPA-induced [Ca2+]i rise was 86 ± 5 nM (n=9; range: 55 – 105 nM).

For the second approach, we examined nuclear translocation of NFATc3-GFP in response to [Ca2+]i increases induced by the Ca2+ ionophore ionomycin (10 μM; Supplemental Fig. 6). In order to limit the [Ca2+]i rise anywhere in the cell, we set the extracellular Ca2+ concentration to either ~170 or 255 nM by mixing 5 mM EGTA with either 4.3 or 4.4 mM CaCl2, respectively, in the extracellular buffer (see Materials and Methods for details). We also added 1 mM La3+ to the extracellular solution to block Ca2+ extrusion from the cells (Usachev et al., 1993). Since ionomycin can also release Ca2+ from the ER Ca2+ stores, DRG neurons were pretreated with 5 μM CPA for at least 30 min to deplete intracellular Ca2+ stores prior to the application of ionomycin and La3+. Under these conditions, the protonophore p-trifluoromethoxyphenylhydrazone (FCCP; 1 μM) did not induce any increase in [Ca2+]i (n=6, not shown), suggesting that mitochondria were nearly empty. As shown in Supplemental Figure 6A and 6B, treating neurons with the combination of ionomycin/La3+ essentially clamped [Ca2+]i at the expected levels set by the extracellular Ca2+ concentration. More importantly, these ionomycin-induced [Ca2+]i elevations were sufficient to trigger nuclear import of NFATc3 in all DRG neurons tested (n=17; Supplemental Fig. 6).

Thus, three different approaches to increase [Ca2+]i - high-K+ depolarization, CPA application or ionomycin/La3+ treatment - produced similar results, demonstrating the [Ca2+]i elevation to 200–300 nM is sufficient to evoke nuclear translocation of NFAT in DRG neurons.

In order to examine the [Ca2+]i dependence of the transcriptional activity mediated by the endogenous NFATc proteins, we transfected DRG neurons with the NFAT-luciferase (firefly) reporter gene. Cells were stimulated by elevating [K+]o to 10, 15, 20 or 25 mM for 12 hours, and luciferase activity was subsequently analyzed (relative light units; Firefly RLU). The activity of firefly luciferase was normalized to the activity of co-transfected constitutively expressed Renilla luciferase (Renilla RLU). [K+]o elevation to 15 mM and higher produced a robust increase in the expression of the NFAT-luciferase reporter (Fig. 3E). In parallel experiments, we also measured the corresponding changes in [Ca2+]i induced by prolonged elevation of [K+]o under conditions similar to those used for the NFAT-luciferase reporter assays (Fig. 3E and Supplemental Fig. 4). [Ca2+]i measurements were made at 2, 7 and 12 hrs after the beginning of high-K+ stimulation. Subsequently, NFAT-luciferase activity was plotted as a function of [Ca2+]i, which revealed a very steep [Ca2+]i dependence of the NFAT-mediated transcription response in sensory neurons (Fig. 3F).

Taken together, these data indicate that NFAT activation in DRG neurons requires an elevation of [Ca2+]i to above 200–300 nM, which is similar to or lower than the [Ca2+]i levels attained during the [Ca2+]i plateau phase described in Figure 2.

Depolarization-induced NFAT activation depends on mitochondrial Ca2+ cycling

Next, we examined the role of mitochondrial Ca2+ uptake/release in the regulation of NFAT. DRG neurons were stimulated by two 30 s pulses of 90 mM KCl separated by a 90 min interval, which enabled eliciting highly reproducible [Ca2+]i responses with a characteristic [Ca2+]i plateau phase, and also allowed repeated activation of NFAT in the same cell (Fig. 4A). First, we used this paired-pulse protocol to examine the effect of the electron chain inhibitor antimycin A1 (1 μM) that depolarizes mitochondria and blocks mitochondrial Ca2+ uptake (Nicholls and Budd, 2000). In order to prevent ATP depletion via the reversal mode of the mitochondrial F1, F0 ATP-synthase (Nicholls and Budd, 2000), we always used antimycin together with the ATP-synthase inhibitor oligomycin (5 μM). In the presence of oligomycin, glycolysis maintains the ATP levels in cultured neurons for tens of minutes after the dissipation of electrochemical gradient (Nicholls and Budd, 2000) without impairment of ATP-dependent Ca2+ transport (Usachev et al., 2002) or cell viability (Budd and Nicholls, 1996).

Figure 4
Mitochondria facilitate NFAT activation induced by strong depolarization

Combined treatment with antimycin and oligomycin (Ant/Ol) increased the amplitude of the [Ca2+]i response and eliminated the [Ca2+]i plateau phase, in both the cytosol and the nucleus (Fig. 4A, B). More importantly, this blockade of mitochondrial Ca2+ uptake markedly decreased nuclear translocation of NFATc3 (Fig. 4B). To quantify the Ant/Ol effect on NFATc3 activation, we calculated the magnitude of GFP-NFATc3 translocation induced by K+90 as ΔRNFAT=Rpeak−Rrest, where Rrest and Rpeak are the nucleus/cytosol ratios of GFP-NFATc3 at rest and at the maximum of NFATc3 translocation, respectively. For each paired-pulse experiment, the degree of NFATc3 translocation induced by the second K+ 90 application (ΔR2NFAT) was normalized to that induced by the first K+ 90 pulse (ΔR1NFAT). We found that ΔR2NFAT was 81 ± 5 % ofΔR1NFAT (n=7) under control conditions (Fig. 4A), and that it was dramatically decreased by the Ant/Ol treatment (ΔR2NFAT/ΔR1NFAT=32 ± 7 %; n=5; p<0.01; Fig. 4B, D).

We also performed control experiments (Supplemental Fig. 7), in which the duration of a small (<300–500 nM) [Ca2+]i elevation was set by the duration of a mild depolarization (15 mM KCl, 20 min), independent of mitochondria. Indeed, [Ca2+]i rapidly returned to the basal levels upon the wash-out of the K+15 solution (Supplemental Fig. 7A), and the duration of the [Ca2+]i response was not changed by the Ant/Ol treatment (Supplemental Fig. 7B). Notably, for this protocol NFATc3 translocation was not affected by the combined addition of 1 μM antimycin and 5 μM oligomycin (Supplemental Fig. 7C), suggesting that these drugs did not affect NFAT transport itself, downstream of the [Ca2+]i elevation.

Another way to disrupt mitochondrial Ca2+ cycling is to block Ca2+ release from mitochondria, which is mediated by the mitochondrial Na+/Ca2+ exchanger in neurons (David, 1999; Colegrove et al., 2000; Thayer et al., 2002; Garcia-Chacon et al., 2006; Medvedeva et al., 2008). Therefore we tested the effect of the exchange inhibitor CGP37157 on NFATc3 translocation in DRG neurons using the paired-pulse protocol described above. By monitoring [Ca2+]mt in mtPericam-transfected neurons, we confirmed the ability of CGP37157 to effectively and reversibly block Ca2+ release from mitochondria in our system (Supplemental Fig. 8). Given that CGP37157 can interfere with depolarization-induced Ca2+ influx (Baron and Thayer, 1997), this agent was applied after washing out the K+90-containing solution. Treatment with 10 μM CGP37157 eliminated the [Ca2+]i plateau phase, in both the cytosol and the nucleus, and markedly suppressed nuclear translocation of NFATc3 (Fig. 4C, D). The ΔR2NFAT/ΔR1NFAT ratio was reduced from 81 ± 5 % (n=7) in control to 55 ± 9 % (n=7; p<0.05; Fig. 4D).

We also examined the role of mitochondria in the regulation of other NFAT isoforms expressed in DRG neurons, such as NFATc1, NFATc2 and NFATc4 ((Fig. 1A, B) and (Jung and Miller, 2008)), using approaches similar to those described for the NFATc3 isoform. Supplemental Figure 9 shows simultaneous recording of [Ca2+]i and EGFP-NFATc1 (A–C), EGFP-NFATc2 (D–F) or EGFP-NFATc4 (G) in individual DRG neurons. K+90-induced depolarization (30 s) triggered rapid nuclear translocation of NFATc1 and NFATc2 (Supplemental Fig. 9A, D). However only minute nuclear import of NFATc4 was observed in response to depolarization, even after stimulating cells by three 3-min applications of 90 mM KCl (Supplemental Fig. 9G). It is possible that NFATc4 is preferentially activated by NGF in DRG neurons (Groth et al., 2007). As for NFATc3, blockade of mitochondrial Ca2+ uptake or release strongly reduced nuclear translocation of both NFATc1 and NFATc2 (Supplemental Fig. 9).

Taken together, these data demonstrate that disruption of mitochondrial Ca2+ cycling by blocking either Ca2+ uptake or release strongly suppresses the depolarization-induced nuclear import of NFAT in DRG neurons. These experiments also emphasize the capability of mitochondria to adjust the size and duration of the Ca2+ response to suit the activation requirements of calcineurin and the transcription factor NFAT.

Mitochondria facilitate transcription mediated by the endogenous NFATc proteins in DRG neurons

Having established that mitochondria contribute to the nuclear translocation of NFAT isoforms, we tested whether transcription mediated by the endogenous NFATc proteins also depends on these organelles. In order to rule out a potential contribution of non-neuronal cells that are also present in DRG cultures, a single-cell NFAT reporter assay was performed in these experiments. For this method, cells were transfected with an NFAT-EGFP reporter that enables monitoring NFAT-dependent expression of EGFP in single identified DRG neurons. DRG cultures were stimulated using three applications of K+90 (90 mM KCl, 3 min, 40 min between applications), and 12 hrs later, EGFP expression was quantified in individual DRG neurons (Fig. 5A, B) by two methods: 1) counting the number of EGFP-positive cells (Fig. 5C, black bars), and 2) calculating the total EGFP fluorescence in all EGFP-positive cells (ΣFEGFPi; Fig. 5C, green bars). We chose to use CGP37157 to disrupt mitochondrial Ca2+ cycling in these experiments because, in contrast to the effects of Ant/Ol, those of CGP37157 are rapidly reversed upon drug removal ((Medvedeva et al., 2008) and Supplemental Fig. 8), which enables treatments of specified duration. CGP37157 (3 μM) was applied for 32 min immediately after K+90 removal. K+90-depolarization increased the number of EGFP-positive DRG neurons and the total EGFP fluorescence by approximately 2.8-fold compared to those in control (unstimulated) cells (Fig. 5C). Inhibition of mitochondrial Ca2+ release by CGP37157 significantly reduced both the number of EGFP-positive neurons and the total EGFP fluorescence (Fig. 5C; n=6 independent experiments). In contrast, CGP37157 had no effect on the number of EGFP-expressing neurons transfected with constitutive EGFP that is controlled by the CMV promoter (n=3 independent experiments; not shown), indicating that the CGP37157 effect is not due to toxicity.

Figure 5
Mitochondrial Ca2+ release facilitates transcriptional response mediated by endogenous NFAT

The effect of mitochondria on NFAT activation depends on the Ca2+ extrusion mechanisms in DRG neurons

Next, we tested the involvement of mitochondria in NFAT activation by bursts of action potentials or TRPV1 agonist using a paired-pulse protocol similar to that described in Figure 4. Repeated stimulation by either a train of action potentials (10 Hz, 10 s; Fig. 6A, B) or the TRPV1 agonist capsaicin (200 nM, 30 s; Fig. 6C, D) evoked [Ca2+]i and NFATc3 responses of reproducible size and duration (Fig. 6A, C). Treating cells with 1 μM antimycin and 5 μM oligomycin eliminated the [Ca2+]i plateau and increased the peak [Ca2+]i elevation. Blockade of mitochondrial Ca2+ uptake also markedly reduced the nuclear translocation of NFATc3 for both stimulation protocols (Fig. 6A–E).

Figure 6
Mitochondria facilitate NFATc3 activation by action potentials and TRPV1 agonist

The height and the duration of [Ca2+]i plateau are defined by the balance between mitochondrial Ca2+ release and Ca2+ clearance (Colegrove et al., 2000). It is conceivable that factors that affect the Ca2+ extrusion machinery also modulate the plateau duration and the degree of NFAT activation in neurons. To test this idea, we blocked plasma membrane Na+/Ca2+ exchange (NCX) by removing extracellular Na+ (Herrington et al., 1996; Lu et al., 2006) and the activity of the plasma membrane Ca2+-ATPase (PMCA) by raising the extracellular pH to 8.8 (Schwiening et al., 1993; Usachev et al., 2002). This combined treatment essentially stalled [Ca2+]i recovery for as long as Ca2+ extrusion remained inhibited (Fig. 6F), and return to the normal extracellular buffer rapidly restored [Ca2+]i recovery to the baseline level. Notably, the extension of the [Ca2+]i plateau achieved by inhibiting NCX and PMCA also markedly enhanced nuclear import of NFATc3 (n=5), as predicted by our hypothesis.

Mitochondria facilitate the function of NFAT as a nuclear integrator of repetitive neuronal activity

The nuclear export of NFAT occurs with a substantial delay following termination of the [Ca2+]i signal. As a result, repetitive [Ca2+]i spikes can lead to temporal summation of NFAT responses within the nucleus, enabling NFAT to function as “a working memory of Ca2+ signals” (Tomida et al., 2003). In sympathetic neurons, [Ca2+]i transients induced by repetitive frequent bursts of action potentials (50 s between the bursts) led to summation and accumulation of NFATc1 in the nucleus (Hernandez-Ochoa et al., 2007). We observed a similar summation phenomenon for NFATc3 in DRG neurons in response to trains of action potentials separated by 60 s (Fig. 2B). To further test the longevity of NFAT “memory” DRG neurons were stimulated by trains of action potentials (10 Hz for 10 s) spaced 15 min apart (Fig. 7A, B). Under control conditions, [Ca2+]i responses showed the characteristic mitochondria-dependent hump during the recovery phase. The nuclear level of NFATc3 rapidly increased after the first stimulus and remained elevated between the stimuli. In contrast, a combined treatment with antimycin and oligomycin eliminated the [Ca2+]i hump and markedly suppressed the NFATc3 response, so that the nuclear/cytosolic ratio fully recovered before each subsequent stimulation (Fig. 7B). Thus, mitochondria contribute to the temporal summation of NFAT responses induced by repetitive neuronal activity.

Figure 7
Mitochondria facilitate temporal summation of repetitive electrical activity by NFAT

To gain further insight into NFAT regulation in neurons, we plotted the extent of NFATc3 nuclear translocation as a function of the duration of the [Ca2+]i response for all the data obtained using various protocols and treatments (Figs. 2, ,44 and and6).6). As shown in Figure 7C, this relationship was well described by the linear function (correlation coefficient r2=0.96) across the broad range of stimulation protocols and pharmacological treatments, suggesting that the duration of NFAT residence in the nucleus is determined by the duration of [Ca2+]i elevation. This also indicates that the mitochondrial inhibitors and the treatments targeting Ca2+ extrusion acted selectively at the level of Ca2+ signal generation, but that they did not directly affect downstream signaling steps such as nuclear import or export of NFAT.

DISCUSSION

Ca2+ is the central regulator of excitation-transcription coupling in neurons (West et al., 2002; Fields et al., 2005). To date, most research has focused on the role of Ca2+ entry mechanisms in controlling specific transcription responses triggered by neuronal activity (reviewed in (Deisseroth et al., 2003; Cohen and Greenberg, 2008)). We now present evidence that an additional level of transcriptional control is provided by systems that continue to shape Ca2+ signals even after Ca2+ entry is terminated. These systems include Ca2+ pumps and exchangers and Ca2+-transporting organelles. Although all of these systems were known to significantly influence the spatial and temporal characteristics of neuronal Ca2+ signals (Miller, 1991; Thayer et al., 2002), their specific involvement in the control of neuronal gene expression remained largely unexplored.

Here we demonstrate for the first time that prolonged Ca2+ release from mitochondria facilitates activation of the transcription factor NFAT in neurons. Moreover, we show that this effect additionally depends on Ca2+ extrusion via the plasma membrane Ca2+ pump and Na+/Ca2+ exchanger. Based on our findings, we propose a model that incorporates mitochondrial Ca2+ cycling acting in concert with other Ca2+ transporters as a novel regulatory mechanism of excitation-transcription coupling in neurons (Fig. 7D). According to this model, the nuclear import of NFAT is triggered by Ca2+ influx via voltage- or ligand-gated Ca2+ channels. Ca2+ entering the cell is rapidly buffered by mitochondria and then is slowly released back to the cytosol via the mitochondrial Na+/Ca2+ exchanger. The resulting prolonged [Ca2+]i plateau, which is set by the balance between mitochondrial Ca2+ release and PMCA- and NCX-mediated Ca2+ extrusion, significantly extends NFAT residence within the nucleus, thereby facilitating NFAT transcriptional responses in neurons.

The critical role of NFAT in the nervous system is supported by a number of recent studies. NFAT is involved in the regulation of axonal growth, dendritic branching and presynaptic differentiation (Yoshida and Mishina, 2005; Nguyen and Di Giovanni, 2008; Schwartz et al., 2009), and combined deletion of either NFATc3/NFATc4 or NFATc2/NFATc3/NFATc4 isoforms leads to a marked deficiency in axonal development (Graef et al., 2003). Moreover, NFATc2/NFATc4 double-knockout mice show pronounced cognitive, behavioral and neuromuscular defects that are reminiscent of the Down’s syndrome phenotype (Arron et al., 2006). NFATc3 and NFATc4 have also been implicated in the regulation of neuronal survival (Benedito et al., 2005; Jayanthi et al., 2005; Luoma and Zirpel, 2008). Another likely function of NFAT is the long-term regulation of neuronal excitability. Indeed, NFAT regulates the expression of several K+ channels, including voltage-gated Kv1.5, Kv2.1, Kv4.2 and Kv4.3 channels and the small conductance Ca2+-activated KCa2.3 (SK3) channel (Sun et al., 2001; Amberg et al., 2004; Rossow et al., 2004), which are involved in the control of excitability and spike-frequency adaptation in the central and peripheral neurons (Gutman et al., 2005). Notably, the expression of Kv1.5, Kv2.1, Kv4.2 and Kv4.3 appears to be controlled specifically by the NFATc3 isoform, at least in cardiac muscles (Rossow et al., 2004). In sensory neurons, NFAT mediates the expression of several pro-nociceptive genes, including COX-2, BDNF and the chemokine receptor CCR2 (Groth et al., 2007; Jackson et al., 2007; Jung and Miller, 2008). COX-2 and BDNF have been linked to inflammatory pain (Svensson and Yaksh, 2002; McMahon et al., 2006), whereas CCR2 upregulation may contribute to the development of neuropathic pain (Jung and Miller, 2008). Therefore, the regulation of nociceptive plasticity is yet another function of NFAT.

Despite the importance of NFAT in the central and peripheral nervous systems, many questions remain about the mechanisms of NFAT regulation in neurons. By utilizing simultaneous monitoring of [Ca2+]i and NFAT translocation, we now provide new information on several important aspects of NFAT signaling in neurons. First, we found that an action potential-induced [Ca2+]i increase triggers nuclear import of NFAT in sensory neurons with a relatively short delay of 30–40 s (Figs. 2A, B). This is consistent with the finding that more than 50% of NFAT becomes dephosphorylated within one minute of stimulation with Ca2+ (Tomida et al., 2003). Second, we quantified NFAT activation as a function of [Ca2+]i increase. We found that rather small [Ca2+]i elevations (~200–300 nM) were sufficient to activate NFAT in DRG neurons. Importantly, several independent assays revealed similar [Ca2+]i dependences. This includes examination of NFAT nuclear import in response to [Ca2+]i elevations elicited by depolarization, cyclopiazonic acid or ionomycin (Fig. 3A–D and Supplemental Figs. 3, 5 and 6), and the analysis of NFAT-luciferase expression mediated by endogenous NFATc proteins (Fig. 3E, F). The observed very steep, threshold-like [Ca2+]i-dependence of NFAT activation (Fig. 3F) is in good agreement with the highly cooperative Ca2+ dependence of CaN activation by calmodulin (Stemmer and Klee, 1994). Third, we found that induction of NFAT response depended on the activity of superoxide dismutase (Fig. 1F), an enzyme that protects CaN from superoxide radicals produced by mitochondria during a prolonged [Ca2+]i elevation (Bito et al., 1996; Wang et al., 1996; Hongpaisan et al., 2003, 2004).

Most importantly, we found that prolonged Ca2+ release from mitochondria facilitates NFAT activation in sensory neurons. NFAT is exquisitely sensitive to the duration of the Ca2+ signal (Dolmetsch et al., 1997; Feske et al., 2001), which is imposed by the competition between CaN and NFAT kinases in controlling nuclear-cytosolic shuttling of NFAT. Ca2+/CaN-dependent dephosphorylation of NFAT exposes the NLS and facilitates nuclear import of NFAT (Crabtree and Olson, 2002; Hogan et al., 2003). Upon termination of the [Ca2+]i response, CaN dissociates from NFAT, allowing GSK3β and other NFAT kinases to rephosphorylate the transcription factor, thereby facilitating Crm-1-dependent nuclear export of NFAT (Beals et al., 1997; Chow et al., 1997; Okamura et al., 2000). Thus, a prolonged [Ca2+]i elevation is required to sustain CaN activation and to induce the NFAT-mediated transcriptional response.

In immune and other non-excitable cells, prolonged [Ca2+]i elevation is enabled by a capacitative Ca2+ influx via CRAC channels (Feske et al., 2006; Putney, 2007). Our work now demonstrates that in neurons, a similar role in the NFAT activation process can be attributed to the mitochondrion. By steadily releasing Ca2+ accumulated during neuronal stimulation, this organelle markedly extends the elevation in [Ca2+]i beyond the short duration of an action potential train or TRPV1 activation, and the [Ca2+]i levels during the mitochondria-dependent plateau phase are well within the range of those required for NFAT activation. Interestingly, in T-lymphocytes mitochondria can also contribute to the regulation of NFAT, although through a different mechanism (Hoth et al., 2000; Chen et al., 2007). Whereas NFAT activation in sensory neurons is facilitated directly by the release of Ca2+ from mitochondria (Figs. 4 and and5),5), in T-lymphocytes, the underlying mechanism seems to be Ca2+ buffering by mitochondria and, consequently, a prevention of Ca2+-dependent inactivation of CRAC channels (Hoth et al., 2000). In addition, it was reported that genetic disruption of the mitochondrial respiratory chain prevents NFAT-dependent heart formation in Xenopus laevis and inhibits NFAT signaling in HeLa and Jurkat cells, most likely as a result of ATP depletion (Chen et al., 2007). It is unlikely that the ATP depletion contributed appreciably to our findings, however. First, in our work the electron transport inhibitor antimycin was always applied in the presence of the ATP synthase inhibitor oligomycin. Such combined treatment produces mitochondrial depolarization without a loss of ATP, as ATP can be maintained in cultured neurons via glycolysis (Nicholls and Budd, 2000) (see also Supplemental Figure 7). Second, we found that an inhibitor of mitochondrial Na+/Ca2+ exchange, CGP37157, that is known to increase cellular ATP production (Visch et al., 2004), also eliminated the Ca2+ plateau and inhibited NFAT activation in DRG neurons, similarly to the effects of antimycin/oligomycin treatment.

Our mtPericam [Ca2+]mt measurements indicated that mitochondria throughout the cell body were engaged in Ca2+ storage and release, including mitochondria adjacent to the nucleus (Fig. 2E, F). Ca2+ released from perinuclear mitochondria was the likely source of the prolonged [Ca2+]i elevation within the nucleus, which could be important for maintaining the CaN-NFAT interaction, and thus, for preventing Crm-1-mediated nuclear export of NFAT (Zhu and McKeon, 1999). Pharmacological disruption of mitochondrial Ca2+ transport eliminated the [Ca2+]i plateau in both the cytosol and the nucleus, and significantly diminished NFAT nuclear import and NFAT-mediated transcription. Furthermore, mitochondrial inhibitors prevented the temporal summation of nuclear NFAT responses induced by repetitive bursts of action potentials (Fig. 7).

Taken together, our data suggest that Ca2+ cycling by mitochondria promotes NFAT activation in neurons and facilitates the function of NFAT as a nuclear integrator of repetitive electrical activity. Given the ability of mitochondria to markedly reduce the magnitude and prolong the duration of Ca2+ signals, and to affect propagation of Ca2+ elevation to the nucleus (Park et al., 2001), it is likely that the described here mechanism is of general importance for neuronal gene regulation, and that many other Ca2+-dependent transcription factors (e.g., CREB, CaRF and MEF2) will also be differentially modulated by mitochondria. The net result will ultimately be defined by the sensitivity of a specific transcription factor to the amplitude and duration of Ca2+ signals, and by its proximity to mitochondria and other competing Ca2+ buffers.

Supplementary Material

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Acknowledgments

This work was supported by National Institutes of Health grant NS054614 and American Heart Association National Scientist Development Grant to Y.M.U. M.S.K. is a predoctoral fellow of the American Heart Association, Midwest Affiliate. We thank Dr. Thayer and Dr. Mermelstein for their support and help in the initiation of this work, Dr. Hell and Dr. Strack for helpful comments on this manuscript, Dr. Rao for the EGFP-NFAT1 (c2) plasmid, Dr. Olson for the EGFP-NFAT2 (c1) plasmid, Dr. Iino for the GFP-NFAT4 (c3) plasmid, Dr. Graef for the EGFP-NFATc4 plasmid and Dr. Miyawaki for the mtPericam plasmid.

References

  • Amberg GC, Rossow CF, Navedo MF, Santana LF. NFATc3 regulates Kv2.1 expression in arterial smooth muscle. J Biol Chem. 2004;279:47326–47334. [PubMed]
  • Aramburu J, Yaffe MB, Lopez-Rodriguez C, Cantley LC, Hogan PG, Rao A. Affinity-driven peptide selection of an NFAT inhibitor more selective than cyclosporin A. Science. 1999;285:2129–2133. [PubMed]
  • Arron JR, Winslow MM, Polleri A, Chang CP, Wu H, Gao X, Neilson JR, Chen L, Heit JJ, Kim SK, Yamasaki N, Miyakawa T, Francke U, Graef IA, Crabtree GR. NFAT dysregulation by increased dosage of DSCR1 and DYRK1A on chromosome 21. Nature. 2006;441:595–600. [PubMed]
  • Baron KT, Thayer SA. CGP37157 modulates mitochondrial Ca2+ homeostasis in cultured rat dorsal root ganglion neurons. Eur J Pharmacol. 1997;340:295–300. [PubMed]
  • Beals CR, Sheridan CM, Turck CW, Gardner P, Crabtree GR. Nuclear export of NF-ATc enhanced by glycogen synthase kinase-3. Science. 1997;275:1930–1934. [PubMed]
  • Benedito AB, Lehtinen M, Massol R, Lopes UG, Kirchhausen T, Rao A, Bonni A. The transcription factor NFAT3 mediates neuronal survival. J Biol Chem. 2005;280:2818–2825. [PubMed]
  • Bhave G, Gereau RWt. Posttranslational mechanisms of peripheral sensitization. J Neurobiol. 2004;61:88–106. [PubMed]
  • Bito H, Deisseroth K, Tsien RW. CREB phosphorylation and dephosphorylation -a Ca2(+)- and stimulus duration-dependent switch for hippocampal gene expression. Cell. 1996;87:1203–1214. [PubMed]
  • Brosenitsch TA, Katz DM. Physiological patterns of electrical stimulation can induce neuronal gene expression by activating N-type calcium channels. J Neurosci. 2001;21:2571–2579. [PubMed]
  • Budd SL, Nicholls DG. A reevaluation of the role of mitochondria in neuronal Ca2+ homeostasis. J Neurochem. 1996;66:403–411. [PubMed]
  • Camello C, Lomax R, Petersen OH, Tepikin AV. Calcium leak from intracellular stores--the enigma of calcium signalling. Cell Calcium. 2002;32:355–361. [PubMed]
  • Caterina MJ, Julius D. The vanilloid receptor: a molecular gateway to the pain pathway. Annu Rev Neurosci. 2001;24:487–517. [PubMed]
  • Chen Y, Yuen WH, Fu J, Huang G, Melendez AJ, Ibrahim FB, Lu H, Cao X. The mitochondrial respiratory chain controls intracellular calcium signaling and NFAT activity essential for heart formation in Xenopus laevis. Mol Cell Biol. 2007;27:6420–6432. [PMC free article] [PubMed]
  • Chin ER, Olson EN, Richardson JA, Yang Q, Humphries C, Shelton JM, Wu H, Zhu W, Bassel-Duby R, Williams RS. A calcineurin-dependent transcriptional pathway controls skeletal muscle fiber type. Genes Dev. 1998;12:2499–2509. [PubMed]
  • Chow CW, Rincon M, Cavanagh J, Dickens M, Davis RJ. Nuclear accumulation of NFAT4 opposed by the JNK signal transduction pathway. Science. 1997;278:1638–1641. [PubMed]
  • Cohen S, Greenberg ME. Communication between the synapse and the nucleus in neuronal development, plasticity, and disease. Annu Rev Cell Dev Biol. 2008;24:183–209. [PMC free article] [PubMed]
  • Colegrove SL, Albrecht MA, Friel DD. Dissection of mitochondrial Ca2+ uptake and release fluxes in situ after depolarization-evoked [Ca2+]i elevations in sympathetic neurons. J Gen Physiol. 2000;115:351–369. [PMC free article] [PubMed]
  • Crabtree GR, Olson EN. NFAT signaling: choreographing the social lives of cells. Cell. 2002;109(Suppl):S67–79. [PubMed]
  • David G. Mitochondrial clearance of cytosolic Ca2+ in stimulated lizard motor nerve terminals proceeds without progressive elevation of mitochondrial matrix [Ca2+] J Neurosci. 1999;19:7495–7506. [PubMed]
  • Deisseroth K, Mermelstein PG, Xia H, Tsien RW. Signaling from synapse to nucleus: the logic behind the mechanisms. Curr Opin Neurobiol. 2003;13:354–365. [PubMed]
  • Dolmetsch RE, Lewis RS, Goodnow CC, Healy JI. Differential activation of transcription factors induced by Ca2+ response amplitude and duration. Nature. 1997;386:855–858. [PubMed]
  • Eshete F, Fields RD. Spike frequency decoding and autonomous activation of ca2+-calmodulin-dependent protein kinase ii in dorsal root ganglion neurons. J Neurosci. 2001;21:6694–6705. [PubMed]
  • Feske S, Giltnane J, Dolmetsch R, Staudt LM, Rao A. Gene regulation mediated by calcium signals in T lymphocytes. Nature Immunology. 2001;2:316–324. [PubMed]
  • Feske S, Gwack Y, Prakriya M, Srikanth S, Puppel SH, Tanasa B, Hogan PG, Lewis RS, Daly M, Rao A. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature. 2006;441:179–185. [PubMed]
  • Fields RD, Lee PR, Cohen JE. Temporal integration of intracellular Ca2+ signaling networks in regulating gene expression by action potentials. Cell Calcium. 2005;37:433–442. [PubMed]
  • Fields RD, Eshete F, Stevens B, Itoh K. Action potential-dependent regulation of gene expression: temporal specificity in Ca2+, cAMP-responsive element binding proteins, and mitogen-activated protein kinase signaling. J Neurosci. 1997;17:7252–7266. [PubMed]
  • Friel DD. Mitochondria as regulators of stimulus-evoked calcium signals in neurons. Cell Calcium. 2000;28:307–316. [PubMed]
  • Garcia-Chacon LE, Nguyen KT, David G, Barrett EF. Extrusion of Ca2+ from mouse motor terminal mitochondria via a Na+-Ca2+ exchanger increases post-tetanic evoked release. J Physiol (Lond) 2006;574:663–675. [PubMed]
  • Graef IA, Mermelstein PG, Stankunas K, Neilson JR, Deisseroth K, Tsien RW, Crabtree GR. L-type calcium channels and GSK-3 regulate the activity of NF-ATc4 in hippocampal neurons. Nature. 1999;401:703–708. [PubMed]
  • Graef IA, Wang F, Charron F, Chen L, Neilson J, Tessier-Lavigne M, Crabtree GR. Neurotrophins and netrins require calcineurin/NFAT signaling to stimulate outgrowth of embryonic axons. Cell. 2003;113:657–670. [PubMed]
  • Greer PL, Greenberg ME. From synapse to nucleus: calcium-dependent gene transcription in the control of synapse development and function. Neuron. 2008;59:846–860. [PubMed]
  • Groth RD, Mermelstein PG. Brain-derived neurotrophic factor activation of NFAT (nuclear factor of activated T-cells)-dependent transcription: a role for the transcription factor NFATc4 in neurotrophin-mediated gene expression. J Neurosci. 2003;23:8125–8134. [PubMed]
  • Groth RD, Coicou LG, Mermelstein PG, Seybold VS. Neurotrophin activation of NFAT-dependent transcription contributes to the regulation of pro-nociceptive genes. J Neurochem. 2007;102:1162–1174. [PubMed]
  • Grynkiewicz G, Poenie M, Tsien RY. A new generation of Ca2+ indicators with greatly improved fluorescence properties. J Biol Chem. 1985;260:3440–3450. [PubMed]
  • Gutman GA, Chandy KG, Grissmer S, Lazdunski M, McKinnon D, Pardo LA, Robertson GA, Rudy B, Sanguinetti MC, Stuhmer W, Wang X. International Union of Pharmacology. LIII. Nomenclature and molecular relationships of voltage-gated potassium channels. Pharmacol Rev. 2005;57:473–508. [PubMed]
  • Hernandez-Ochoa EO, Contreras M, Cseresnyes Z, Schneider MF. Ca2+ signal summation and NFATc1 nuclear translocation in sympathetic ganglion neurons during repetitive action potentials. Cell Calcium. 2007;41:559–571. [PMC free article] [PubMed]
  • Herrington J, Park YB, Babcock DF, Hille B. Dominant role of mitochondria in clearance of large Ca2+ loads from rat adrenal chromaffin cells. Neuron. 1996;16:219–228. [PubMed]
  • Hogan PG, Chen L, Nardone J, Rao A. Transcriptional regulation by calcium, calcineurin, and NFAT. Genes Dev. 2003;17:2205–2232. [PubMed]
  • Hongpaisan J, Winters CA, Andrews SB. Calcium-dependent mitochondrial superoxide modulates nuclear CREB phosphorylation in hippocampal neurons. Mol Cell Neurosci. 2003;24:1103–1115. [PubMed]
  • Hongpaisan J, Winters CA, Andrews SB. Strong calcium entry activates mitochondrial superoxide generation, upregulating kinase signaling in hippocampal neurons. J Neurosci. 2004;24:10878–10887. [PubMed]
  • Hoth M, Button DC, Lewis RS. Mitochondrial control of calcium-channel gating: A mechanism for sustained signaling and transcriptional activation in T lymphocytes. Proc Natl Acad Sci U S A. 2000;97:10607–10612. [PubMed]
  • Huang SM, Bisogno T, Trevisani M, Al-Hayani A, De Petrocellis L, Fezza F, Tognetto M, Petros TJ, Krey JF, Chu CJ, Miller JD, Davies SN, Geppetti P, Walker JM, Di Marzo V. An endogenous capsaicin-like substance with high potency at recombinant and native vanilloid VR1 receptors. Proc Natl Acad Sci U S A. 2002;99:8400–8405. [PubMed]
  • Jackson JG, Usachev YM, Thayer SA. Bradykinin-induced NFAT-dependent transcription in rat dorsal root ganglion neurons. Mol Pharmacol. 2007;72:303–310. [PubMed]
  • Jayanthi S, Deng X, Ladenheim B, McCoy MT, Cluster A, Cai NS, Cadet JL. Calcineurin/NFAT-induced up-regulation of the Fas ligand/Fas death pathway is involved in methamphetamine-induced neuronal apoptosis. Proc Natl Acad Sci U S A. 2005;102:868–873. [PubMed]
  • Jung H, Miller RJ. Activation of the nuclear factor of activated T-cells (NFAT) mediates upregulation of CCR2 chemokine receptors in dorsal root ganglion (DRG) neurons: a possible mechanism for activity-dependent transcription in DRG neurons in association with neuropathic pain. Mol Cell Neurosci. 2008;37:170–177. [PMC free article] [PubMed]
  • Lu SG, Zhang X, Gold MS. Intracellular calcium regulation among subpopulations of rat dorsal root ganglion neurons. J Physiol (Lond) 2006;577:169–190. [PubMed]
  • Luoma JI, Zirpel L. Deafferentation-induced activation of NFAT (nuclear factor of activated T-cells) in cochlear nucleus neurons during a developmental critical period: a role for NFATc4-dependent apoptosis in the CNS. J Neurosci. 2008;28:3159–3169. [PubMed]
  • McMahon SB, Bennett DL, Bevan S. Inflammatory mediators and modulators of pain. In: McMahon SB, Koltzenburg M, editors. Textbook of Pain. London: Elsevier; 2006. pp. 49–72.
  • Medvedeva YV, Kim MS, Usachev YM. Mechanisms of prolonged presynaptic Ca2+ signaling and glutamate release induced by TRPV1 activation in rat sensory neurons. J Neurosci. 2008;28:5295–5311. [PMC free article] [PubMed]
  • Meyer RA, Campbell JN. Myelinated nociceptive afferents account for the hyperalgesia that follows a burn to the hand. Science. 1981;213:1527–1529. [PubMed]
  • Miller RJ. The control of neuronal Ca2+ homeostasis. Prog Neurobiol. 1991;37:255–285. [PubMed]
  • Nagai T, Sawano A, Park ES, Miyawaki A. Circularly permuted green fluorescent proteins engineered to sense Ca2+ Proc Natl Acad Sci U S A. 2001;98:3197–3202. [PubMed]
  • Nguyen T, Di Giovanni S. NFAT signaling in neural development and axon growth. Int J Dev Neurosci. 2008;26:141–145. [PMC free article] [PubMed]
  • Nicholls DG, Budd SL. Mitochondria and neuronal survival. Physiol Rev. 2000;80:315–360. [PubMed]
  • Nieves-Cintron M, Amberg GC, Navedo MF, Molkentin JD, Santana LF. The control of Ca2+ influx and NFATc3 signaling in arterial smooth muscle during hypertension. Proc Natl Acad Sci U S A. 2008;105:15623–15628. [PubMed]
  • Okamura H, Aramburu J, Garcia-Rodriguez C, Viola JP, Raghavan A, Tahiliani M, Zhang X, Qin J, Hogan PG, Rao A. Concerted dephosphorylation of the transcription factor NFAT1 induces a conformational switch that regulates transcriptional activity. Mol Cell. 2000;6:539–550. [PubMed]
  • Oliveria SF, Dell’Acqua ML, Sather WA. AKAP79/150 anchoring of calcineurin controls neuronal L-type Ca2+ channel activity and nuclear signaling. Neuron. 2007;55:261–275. [PMC free article] [PubMed]
  • Park MK, Ashby MC, Erdemli G, Petersen OH, Tepikin AV. Perinuclear, perigranular and sub-plasmalemmal mitochondria have distinct functions in the regulation of cellular calcium transport. EMBO Journal. 2001;20:1863–1874. [PubMed]
  • Pivovarova NB, Hongpaisan J, Andrews SB, Friel DD. Depolarization-induced mitochondrial Ca accumulation in sympathetic neurons: spatial and temporal characteristics. J Neurosci. 1999;19:6372–6384. [PubMed]
  • Putney JW., Jr Recent breakthroughs in the molecular mechanism of capacitative calcium entry (with thoughts on how we got here) Cell Calcium. 2007;42:103–110. [PMC free article] [PubMed]
  • Rossow CF, Minami E, Chase EG, Murry CE, Santana LF. NFATc3-induced reductions in voltage-gated K+ currents after myocardial infarction. Circ Res. 2004;94:1340–1350. [PubMed]
  • Schnizler K, Shutov LP, Van Kanegan MJ, Merrill MA, Nichols B, McKnight GS, Strack S, Hell JW, Usachev YM. Protein kinase A anchoring via AKAP150 is essential for TRPV1 modulation by forskolin and prostaglandin E2 in mouse sensory neurons. J Neurosci. 2008;28:4904–4917. [PMC free article] [PubMed]
  • Schwartz N, Schohl A, Ruthazer ES. Neural activity regulates synaptic properties and dendritic structure in vivo through calcineurin/NFAT signaling. Neuron. 2009;62:655–669. [PubMed]
  • Schwiening CJ, Kennedy HJ, Thomas RC. Calcium hydrogen exchange by the plasma membrane Ca-ATPase of voltage-clamped snail neurons. Proc R Soc Lond. 1993;253:285–289. [PubMed]
  • Slugg RM, Meyer RA, Campbell JN. Response of cutaneous A- and C-fiber nociceptors in the monkey to controlled-force stimuli. J Neurophysiol. 2000;83:2179–2191. [PubMed]
  • Stemmer PM, Klee CB. Dual calcium ion regulation of calcineurin by calmodulin and calcineurin B. Biochemistry. 1994;33:6859–6866. [PubMed]
  • Sun G, Tomita H, Shakkottai VG, Gargus JJ. Genomic organization and promoter analysis of human KCNN3 gene. J Hum Genet. 2001;46:463–470. [PubMed]
  • Svensson CI, Yaksh TL. The spinal phospholipase-cyclooxygenase-prostanoid cascade in nociceptive processing. Annu Rev Pharmacol Toxicol. 2002;42:553–583. [PubMed]
  • Szallasi A, Cortright DN, Blum CA, Eid SR. The vanilloid receptor TRPV1:10 years from channel cloning to antagonist proof-of-concept. Nat Rev Drug Discov. 2007;6:357–372. [PubMed]
  • Thayer SA, Usachev YM, Pottorf WJ. Modulating Ca2+ clearance from neurons. Front Biosci. 2002;7:D1255–1279. [PubMed]
  • Tomida T, Hirose K, Takizawa A, Shibasaki F, Iino M. NFAT functions as a working memory of Ca2+ signals in decoding Ca2+ oscillation. EMBO J. 2003;22:3825–3832. [PubMed]
  • Usachev Y, Shmigol A, Pronchuk N, Kostyuk P, Verkhratsky A. Caffeine-induced calcium release from internal stores in cultured rat sensory neurons. Neuroscience. 1993;57:845–859. [PubMed]
  • Usachev YM, Thayer SA. Ca2+ influx in resting rat sensory neurones that regulates and is regulated by ryanodine-sensitive Ca2+ stores. J Physiol (Lond) 1999;519:115–130. [PubMed]
  • Usachev YM, DeMarco SJ, Campbell C, Strehler EE, Thayer SA. Bradykinin and ATP accelerate Ca2+ efflux from rat sensory neurons via protein kinase C and the plasma membrane Ca2+ pump isoform 4. Neuron. 2002;33:113–122. [PubMed]
  • Usachev YM, Marsh AJ, Johanns TM, Lemke MM, Thayer SA. Activation of protein kinase C in sensory neurons accelerates Ca2+ uptake into the endoplasmic reticulum. J Neurosci. 2006;26:311–318. [PubMed]
  • Visch HJ, Rutter GA, Koopman WJ, Koenderink JB, Verkaart S, de Groot T, Varadi A, Mitchell KJ, van den Heuvel LP, Smeitink JA, Willems PH. Inhibition of mitochondrial Na+-Ca2+ exchange restores agonist-induced ATP production and Ca2+ handling in human complex I deficiency. J Biol Chem. 2004;279:40328–40336. [PubMed]
  • Wang XT, Culotta VC, Klee CB. Superoxide dismutase protects calcineurin from inactivation. Nature. 1996;383:434–437. [PubMed]
  • West AE, Griffith EC, Greenberg ME. Regulation of transcription factors by neuronal activity. Nat Rev Neurosci. 2002;3:921–931. [PubMed]
  • Yoshida T, Mishina M. Distinct roles of calcineurin-nuclear factor of activated T-cells and protein kinase A-cAMP response element-binding protein signaling in presynaptic differentiation. J Neurosci. 2005;25:3067–3079. [PubMed]
  • Zhu J, McKeon F. NF-AT activation requires suppression of Crm1-dependent export by calcineurin. Nature. 1999;398:256–260. [PubMed]